| 2 | Peptide Functionalized Nanoplasmonic Sensor for Explosive Detection显示文摘In this study, a nanobiosensor for detecting explosives was developed, in which the peptide was synthesized with trinitrotoluene(TNT)-specific sequence and immobilized on nanodevice by Au–S covalent linkage, and the nanocup arrays were fabricated by nanoimprint and deposited with Au nanoparticles to generate localized surface plasmon resonance(LSPR). The device was used to monitor slight change from specific binding of 2,4,6-TNT to the peptide. With high refractive index sensing of ~10~4nm/RIU, the nanocup device can detect the binding of TNT at concentration as low as3.12×10^(-7)mg mL^(-1) by optical transmission spectrum modulated by LSPR. The nanosensor is also able to distinguish TNT from analogs of 2,4-dinitrotoluene and 3-nitrotoluene in the mixture with great selectivity. The peptide-based nanosensor provides novel approaches to design versatile biosensor assays by LSPR for chemical molecules. | Diming Zhang Qian Zhang Yanli Lu Yao Yao Shuang Li Jing Jiang Gang Logan Liu Qingjun Liu | 2016 | Nano-Micro Letters2016，8，1： | 1 |
| 4 | Supplementing N-carbamoylglutamate in late gestation increases newborn calf weight by enhanced placental expression of mTOR and angiogenesis factor genes in dairy cows显示文摘The objective of this study was to investigate whether supplementation with N-carbamoylglutamate(NCG)to cows during late gestation alters uteroplacental tissue nutrient transporters,calf metabolism and newborn weight.Thirty multiparous Chinese Holstein cows were used in a randomized complete block design experiment.During the last 28 d of pregnancy,cows were fed a diet without(CON)or with NCG(20 g/d per cow).The body weight of calves was weighed immediately after birth.Placentome samples were collected at parturition and used to assess mRNA expression of genes involved in transport of arginine,glucose,fatty acid and angiogenesis factors,as well as the mammalian target of rapamycin(mTOR)pathway.Blood samples of calves before colostrum consumption were also collected for the detection of plasma parameters,amino acids(AA)and metabolomics analysis.The newborn weight(P=0.02)and plasma Arg concentration of NCG-calves was significantly higher(P=0.05)than that of CON-calves,and the plasma concentrations of urea nitrogen tended to be lower(P=0.10)in the NCG group.The mRNA abundance of genes involved in glucose transport(solute carrier family 2 member 3[SLC2 A3],P<0.01),angiogenesis(nitric oxide synthase 3[NOS3],P=0.02),and mTOR pathway(serine/threonine-protein kinase 1[ACT1],P=0.10;euka ryotic translation initiation factor 4 B pseudogene 1[EIF4 BP1],P=0.08;EIF4 EBP2,P=0.04;and E74-like factor 2[ELF2],P=0.03)was upregulated in the placentome of NCG-supplemented cows.In addition,17 metabolites were significantly different in the placentome of NCG-supplemented cows compared to non-supplemented cows,and these metabolites are mainly involved in arginine and proline metabolism,alanine,aspartate and glutamate metabolism,and citrate cycle.In summary,the increased body weight of newborn calves from the NCG supplemented dairy cows maybe attributed to the increased angiogenesis and uteroplacental nutrient transport and to the activated mTOR signal pathway,which may result in the increased nutrient supply to the fetus,and improved AA metabolism and urea cycle of the fetus. | Fengfei Gu Luyi Jiang Linyu Xie Diming Wang Fengqi Zhao Jianxin Liu | 2021 | Animal Nutrition2021，，4： | 0 |
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