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| 1 | EGTA reduces the inflorescence stem mechanical strength of herbaceous peony by modifying secondary wall biosynthesis显示文摘The mechanical strength of inflorescence stems is an important trait in cut flowers.Calcium ions(Ca^(2+))play a pivotal role in maintaining stem strength,but little is known about the underlying molecular mechanisms.In this study,we treated herbaceous peony(Paeonia lactiflora Pall.)with ethyl glycol tetraacetic acid(EGTA),an effective Ca^(2+)chelator,and used morphology indicators,spectroscopic analysis,histochemical staining,electron microscopy,and proteomic techniques to investigate the role of Ca^(2+)in inflorescence stem mechanical strength.The EGTA treatment reduced the mechanical strength of inflorescence stems,triggered the loss of Ca^(2+)from cell walls,and reduced lignin in thickened secondary walls in xylem cells as determined by spectroscopic analysis and histochemical staining.Electron microscopy showed that the EGTA treatment also resulted in significantly fewer xylem cell layers with thickened secondary walls as well as in reducing the thickness of these secondary walls.The proteomic analysis showed 1065 differentially expressed proteins(DEPs)at the full-flowering stage(S4).By overlapping the Kyoto encyclopedia of genes and genomes(KEGG)and gene ontology(GO)analysis results,we identified 43 DEPs involved in signal transduction,transport,energy metabolism,carbohydrate metabolism,and secondary metabolite biosynthesis.Using quantitative real-time polymerase chain reaction(qRT-PCR)analysis,we showed that EGTA treatment inhibited Ca^(2+)sensors and secondary wall biosynthesis-related genes.Our findings revealed that EGTA treatment reduced the inflorescence stem mechanical strength by reducing lignin deposition in xylem cells through altering the expression of genes involved in Ca^(2+)binding and secondary wall biosynthesis. | Yuhan Tang Daqiu Zhao Jiasong Meng Jun Tao | 2019 | Horticulture Research2019，6，1： | 2 |
| 2 | Graft copolymers prepared by atom transfer radical polymerization (ATRP) from cellulose显示文摘 | Tao Meng Xia Gao Jun Zhang Jinying Yuan Yuzhu Zhang Jiasong He | 2008 | Polymer2008，，2： | 1 |
| 3 | Mating Ethogram of a Video-aided Study of Mating and Parturition in Captive Chinese Crocodile Lizards (Shinisaurus crocodilurus)显示文摘Mating behavior and parturition are important components in the life history of any species. The endangered Chinese crocodile lizard(Shinisaurus crocodilurus) is suffering dramatic population decreases in the wild. Successful captive breeding can provide individuals to be reintroduced into their natural habitat and help to restore the wild populations. Studying the mating behavior and parturition of these lizards in captivity will enable us understand how is this ancient species reproduction and optimize artificial reproduction protocol. We studied the mating behavior and parturition of Chinese crocodile lizards in captivity using video recordings at the Daguishan National Nature Reserve, China, from 2015 to 2017. We analyzed pre-copulation and copulation, which occurred in mid-March to late May from all studied years. Pre-copulation and copulation lasted on average 7.51(n=11) and 27.45(n=11) minutes, respectively. We found that large-bodied male lizards have longer copulation periods, a pattern seen in other lizard species. In the mating event, males always initiated sex, while the females were passive. The duration of parturition lasted 153.8 ± 26.8(n=46) minutes, and larger females had heavier clutches. In addition, even though Chinese crocodile lizards represent an ancient lizard clade with primitive characteristics, they still abide by the reproduction patterns seen in most other lizard species. | Chengming HUANG Zhengjun WU Shuyi LUO Jiasong HE Chunsheng YANG Meng MENG Lu YAO Zhigao ZENG | 2019 | Asian Herpetological Research2019，10，4： | 1 |
| 4 | Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress显示文摘Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance. | Zhipeng Sheng Jiasong Meng Jun Tao Daqiu Zhao | 2023 | Phyton-International Journal of Experimental Botany2023，92，11： | 0 |
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