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| 1 | Adenovirus-mediated gene delivery:Potential applications for gene and cell-based therapies in the new era of personalized medicine显示文摘With rapid advances in understanding molecular pathogenesis of human diseases in the era of genome sciences and systems biology,it is anticipated that increasing numbers of therapeutic genes or targets will become available for targeted therapies.Despite numerous setbacks,efficacious gene and/or cell-based therapies still hold the great promise to revolutionize the clinical management of human diseases.It is wildly recognized that poor gene delivery is the limiting factor for most in vivo gene therapies.There has been a long-lasting interest in using viral vectors,especially adenoviral vectors,to deliver therapeutic genes for the past two decades.Among all currently available viral vectors,adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types.The applications of adenoviral vectors in gene delivery have greatly increased in number and efficiency since their initial development.In fact,among over 2000 gene therapy clinical trials approved worldwide since 1989,a significant portion of the trials have utilized adenoviral vectors.This review aims to provide a comprehensive overview on the characteristics of adenoviral vectors,including adenoviral biology,approaches to engineering adenoviral vectors,and their applications in clinical and preclinical studies with an emphasis in the areas of cancer treatment,vaccination and regenerative medicine.Current challenges and future directions regarding the use of adenoviral vectors are also discussed.It is expected that the continued improvements in adenoviral vectors should provide great opportunities for cell and gene therapies to live up to its enormous potential in personalized medicine. | Cody S.Lee Elliot S.Bishop Ruyi Zhang Xinyi Yu Evan M.Farina Shujuan Yan Chen Zhao Zongyue Zeng Yi Shu Xingye Wu Jiayan Lei Yasha Li Wenwen Zhang Chao Yang Ke Wu Ying Wu Sherwin Ho Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Russell R.Reid Tong-Chuan He | 2017 | Genes & Diseases2017，4，2： | 19 |
| 2 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 3 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 4 | The 1 PW/0.1 Hz laser beamline in SULF facility显示文摘In this paper,we report the recent progress on the 1 PW/0.1 Hz laser beamline of Shanghai Superintense Ultrafast Laser Facility(SULF).The SULF-1 PW laser beamline is based on the double chirped pulse amplification(CPA)scheme,which can generate laser pulses of 50.8 J at 0.1 Hz after the final amplifier;the shot-to-shot energy fluctuation of the amplified pulse is as low as 1.2%(std).After compression,the pulse duration of 29.6 fs is achieved,which can support a maximal peak power of 1 PW.The contrast ratio at-80 ps before main pulse is measured to be 2.5×10^-11.The focused peak intensity is improved by optimizing the angular dispersion in the grating compressor.The maximal focused peak intensity can reach 2.7×10^19W/cm2 even with an f/26.5 off-axis parabolic mirror.The horizontal and vertical angular pointing fluctuations in 1 h are measured to be 1.89 and 2.45μrad,respectively.The moderate repetition rate and the good stability are desirable characteristics for lasermatter interactions.The SULF-1 PW laser beamline is now in the phase of commissioning,and preliminary experiments of particle acceleration and secondary radiation under 300–400 TW/0.1 Hz laser condition have been implemented.The progress on the experiments and the daily stable operation of the laser demonstrate the availability of the SULF-1 PW beamline. | Zongxin Zhang Fenxiang Wu Jiabing Hu Xiaojun Yang Jiayan Gui Penghua Ji Xingyan Liu Cheng Wang Yanqi Liu Xiaoming Lu Yi Xu Yuxin Leng Ruxin Li Zhizhan Xu | 2020 | High Power Laser Science and Engineering2020，8，1： | 2 |
| 5 | Zirconium nitride ( ZrN ) fibers prepared by carbothermal reduction and nitridation of electrospun PVP/zirconium oxychloride composite fibers显示文摘 | Li Jiayan Sun Yan Tan Yi | 2008 | Chemical Engineering Journal2008，144，1： | 1 |
| 6 | Research of boron removal from polysilicon using CaO–Al 2 O 3 –SiO 2 –CaF 2 slags显示文摘 | Jiayan Li Lei Zhang Yi Tan Dachuan Jiang Dengke Wang Yaqiong Li | 2014 | Vacuum2014，，： | 1 |
| 7 | Preparation and biological activity of quaternized carboxymethylchitosan conjugated with collagen peptide显示文摘 | Xiaoming Zhu Xiaoyu Zhou Jiayan Yi | 2014 | International Journal of Biological Macromolecules2014，70，： | 1 |
| 8 | Influence of heat treatment on microstructure and mechanical properties of Al/Al-Cu graded materials显示文摘Five-layered Al/Al-Cu functionally graded material (FGM) was prepared by powder metallurgy technology, and the subsequent heat treatment was carried out for the graded material. The microstructures and distribution of Cu element under pressure sintering (F), solution treatment (T4) and artificial aging treatment (T6) were investigated, and the Vickers hardness and flexural properties of different states were tested. The results showed that sintered compact with dense structure and compositional continuous change was obtained. The second-phase CuAl 2 was dispersively distributed along grain boundary of Al matrix. After solution treatment at 503 C for 3 h, CuAl 2 phase obviously decreased and dissolved into the Al matrix, and the flexural strength was thereupon enhanced to 228.5 MPa. With the subsequent aging treatment at 150 C for 15 h, the majority of flake shaped precipitates θ phases were uniformly distributed in the matrix. And the distribution of Cu element became gradual continuous compared to sintered compact. Meanwhile, the flexural strength increased further, which accompanied with the decline of plasticity. | Qian CONG Fumin XU Yi TAN Xiaolei SHI Wei DONG Jiayan LI Junshan ZHANG | 2011 | Acta Metallurgica Sinica(English Letters)2011，24，2： | 1 |
| 9 | Effect of the addition of TEOS on the SiC fibers fabricated by electrospinning显示文摘Polycarbosilane(PCS)has been widely used to fabricate silicon carbide(SiC)fibers via pyrolysis.In this paper,for improving the morphology of SiC fibers,tetraethyl orthosilicate(TEOS,m=1 g,3 g and 5 g,respectively)was added into the PCS precursor solution(containing 1.5 g PCS).The continuous fibers have been prepared by electrospinning,and then the SiC fibers were synthesized by calcination at 1300℃,1400℃and 1600℃for 4 h respectively with a heating rate of 10℃/min in flowing nitrogen(N_(2)).The morphologies of the fibers were investigated by the scanning electron microscope(SEM)and it could be seen that the crystallinity of the SiC fibers was lower,the length of the SiC fibers was increased,and the diameter was uniform with the increase of the addition amount of TEOS. | Jiayan LI Panpan CAO Yi TAN Lei ZHANG | 2013 | Journal of Advanced Ceramics2013，2，3： | 1 |
| 10 | Preparation and biological activity of quatemized carboxymethyl chitosan conjugated with collagen peptide显示文摘 | Zhu Xiaoming Zhou Xiaoyu Yi Jiayan | 2014 | Intemational Journal of Biological Macromolecules2014，70，8： | 1 |
| 11 | Fabrication of a turbine disk alloy by electron beam sheet lamination and investigation on the improved high temperature creep performance显示文摘A turbine disk alloy was prepared by electron beam sheet lamination(EBSL),giving a refined microstruc-ture and high purity,and the microstructure-property relationship of this alloy has been developed.The alloy showed an outstanding creep life of 1266.3 h at 953 K/500 MPa.Compared with the conventional vacuum induction melted and vacuum arc re-melted alloy,a smaller dendrite arm spacing of less than 23μm was obtained through the EBSL method,which restricted the formation of large inter-dendritic carbides.As a result,the typical intergranular failure mode was observed in the EBSL alloy,whereas the transgranular fracture mode was dominant in the conventional alloy.The inter-dendritic MC carbides formed from the reverse diffusion of Nb are believed to either impede grain boundary sliding or initi-ate cracks,depending on their size and morphology.The tiny grain boundary precipitates of theδphase in the EBSL alloy could reduce the critical stress for microvoid nucleation to 161.07 MPa by decoher-ing the particle/matrix interface,or allowing vacancy condensation near theδinterface,resulting in the formation of grain boundary microcracks.In the conventional alloy,however,the larger-sized MC car-bides which were formed during the solidification process(0.36 vol.%)nucleated the microcracks within the particles,leading to the transgranular fracture.Furthermore,the results indicated that the nanoscaleγ′andγ″precipitates contributed primarily to strengthening and coordinating intragranular deforma-tion during creep,in which the Orowan mechanism and shearing of a/6<211>partial dislocations and a/2<110>doublets were active forγ′andγ″precipitates,respectively. | Xiaogang You Yi Tan Masao Takeyama Pengting Li Yi Li Huixing Zhang Hongyang Cui Chuanyong Cui Yinong Wang Jiayan Li Zhijun Zhang Gengyi Dong Wenjia Xiao | 2023 | Journal of Materials Science & Technology2023，，12： | 0 |
| 12 | 共担与赋能:有温度的社区与住房显示文摘Introduction On Apr.15-16,2023,the 2022 Annual Conference of the Housing and Community Planning Committee of the Urban Planning Society of China(UPSC)was successfully held at the Chongqing Planning Exhibition Gallery,Chongqing.The conference was hosted by the Housing and Community Planning Committee of the UPSC. | 尹稚 王宇 江维 Yu Ying Liu Jiayan Yao Nan Wang Chenghui Fang Xiaotao Wang Ying Xi Wenqin Pan Chenyue Mou Yanchuan Wei Wei Li Yun Xiao Xiao Chen Yulin Zhao Di Liu Wei Zhang Jiuhe Deng Yi | 2023 | China City Planning Review2023，32，4： | 0 |
| 13 | Corrigendum to “The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs” [Genes & Diseases 5 (2018) 62–74]显示文摘The authors regret having an image assembly error in Figure 5Ca,in which the image for the 'Oh dBiFP-AdRFp'group was erroneously duplicated with an overlapping image from the'36h BiFP dBIFP-AdR-simH19'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C. Haydon Hue H. Luu Lan Zhou Russell R. Reid Tong-Chuan He Xingye Wu | 2023 | Genes & Diseases2023，10，2： | 0 |
| 14 | Corrigendum to “Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes (imPODs)” [Genes & Diseases 5 (2018) 137–149]显示文摘The authors regret having an image assembly error in Figure 3A,in which the image for 'imPOD Synaptopodin DAPl stain'groupwas erroneouslyduplicatedwiththe imagefrom the'tsPOD-33C SynaptopodinDAPIstain'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C. Haydon Hue H. Luu Tong-Chuan He Hua Gan | 2023 | Genes & Diseases2023，10，2： | 0 |
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