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| 1 | Temperature dependent expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis显示文摘p34cdc2 and Cyclin B1 are key components of cell cycle controlling machine and are believed to play a fundamental role in gametogenesis. It is also well known that, in scrotal mammals, spermatogenesis depends greatly on the maintenance of comparatively low temperature in the scrotum. To investigate whether the expression of cdc2 and Cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and Cyclin B1 in normal and cryptorchid testis. Results showed that the abdominal temperature had no significant influence on the transcription of cdc2 and Cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes, but it blocked the translation of them. Due to the deficiency of p34cdc2 and Cyclin B1, the spermatogonia and pachytene/diplotene primary spermatocytes were unable to form MPF, hence, they couldn’t undergo karyokinesis. The development of primary spermatocytes was arrested at the G2 to M phase transition. We also found that testosterone could regulate the Cyclin B1 expression in spermatogenic cells. Muscular injection of testosterone could recover spermatogenesis in the unilateral scrotal testis which was influenced by the contralateral cryptorchid testis, but it could not salvage the spermatogenesis block in the cryptorchid testis. | KONG WEI HUA ZHENG GU JI NING LU JIA KE TSO Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China College of Life Science, Shandong University, Jinan 250100, China | 2000 | Cell Research2000，10，4： | 12 |
| 2 | VEGF-specific siRNAs modified with 2′-deoxy effectively suppress VEGF expression and inhibit growth of nasopharyngeal carcinoma xenograft in a mouse model显示文摘Vascular endothelial growth factor (VEGF) is up-regulated in the vast majority of human tumors. The up-regulation of VEGF not only plays important roles in tumor angiogenesis, but also provides a target for tumor treatment with small interfering RNA (siRNA) that targets VEGF; however, it is unclear whether a quite high up-regulation of VEGF will affect the efficiency of RNA interference strategies targeting VEGF. A high level expression of VEGF was found in CNE cells from a nasopharyngeal carcinoma cell line. In this study, we investigate whether VEGF-specific siRNAs can effectively suppress VEGF expression in CNE cells, and study the methods for the use of VEGF-specific siRNAs as potential therapeutic agents. CNE cells with high VEGF expression induced by hypoxia were transfected with VEGF-specific siRNAs. The expression of VEGF was effectively suppressed by VEGF-specific siRNAs, measured by ELISA, Western blot analysis and RT-PCR. Furthermore, experiments in nude mice bearing nasopharyngeal carcinoma xenograft were initiated 5 d after injection of CNE cells. VEGF-specific siRNAs were modified with 2′-deoxy, then injected into the tumors, and a liposome-mediated siRNA transfection system and ultrasound exposure were used to help delivery of the siRNAs. Tumor growth was reduced significantly after 3 weeks' treatment. These studies suggest that VEGF-specific siRNAs still can effectively suppress VEGF expression even in tumor cell lines with a relatively high level of VEGF expression, such as CNE, and VEGF-specific siRNAs modified with 2′-deoxy can be used as potential agents for tumor therapy. | CHEN ShanYi1, GAO GuoFeng1, CHEN Wei1, Lü Qing2, TANG Shen2, HUA Zhong2, YE WenBin2, GU DaYong2, WANG ShaYan1 & ZHANG YaOu2 1 Shenzhen People’s Hospital, Clinical Medical College of Jinan University, Shenzhen 518020, China 2 Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China | 2008 | Science China(Life Sciences)2008，51，2： | 6 |
| 3 | High spin states in stable nucleus ^(84)Sr显示文摘High spin states of 84Sr were populated through the reaction 70Zn(18O,4n)84Sr at 75 MeV beam energy.Measurement of excitation function,γ-γ coincidences,directional correlation from oriented state (DCO) ratios and γ-transition intensities were performed using eight anticompton HPGe detectors and one planar HPGe detector.Based on the measured results,a new level scheme of 84Sr was established in which 12 new states and nearly 30 new γ-transitions were identified in the present work.The positive-parity states of the new level scheme were compared with the results from calculations in the framework of the projected shell model (PSM).One negative-parity band was extended to spin Iπ=19-and it can be found that in the high spin states,the γ-transition energies show the nature of signature staggering.The negative-parity band levels are in good agreement with deformed configuration-mixing shell model (DCM) calculations. | KANG XuZhong1,2,SHEN ShuiFa1,2,3,4*,HAN GuangBing5,PAN Feng3,4,GU JianZhong6,DRAAYER J P4,WU XiaoGuang6,ZHU LiHua6 & WEN TingDun7 1 Key Laboratory of Nuclear Resources and Environment (East China Institute of Technology),Ministry of Education,Nanchang 330013,China 2 School of Nuclear Engineering and Technology,East China Institute of Technology,Fuzhou 344000,China 3 Department of Physics,Liaoning Normal University,Dalian 116029,China 4 Department of Physics and Astronomy,Louisiana State University,Baton Rouge,LA 70803-4001,USA 5 School of Physics,Shandong University,Jinan 250100,China 6 China Institute of Atomic Energy,Beijing 102413,China 7 Department of Physics,North University of China,Taiyuan 030051,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，10： | 4 |
| 4 | Quenching rate constants for metastable molecule PC1(b^1∑^+,v' = 0)显示文摘IN this decade, great attention has been paid to PCl(b1∑+) because of its potential importance in chemical laser. Coxon et al. studied the reaction of Ar(3Po,2) with PCl3 and observed two band systems of PCl(A3Ⅱ→X3∑-) and (b1∑+→X3∑-). Beielefeld et al. and Setser et al. measured the radiative lifetime of PCl(b1∑+) and its quenching rate constants for some small molecule quenchers. Recently, we have prepared a clean source of PCl(b,υ′= O) in | LIU Chuanpu, ZOU Shengli, GUO Jingzhong, GU Yueshu, CAO Dezhao and CHU Yannan Department of Chemistry, Shandong University, Jinan 250100, China Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China | 1997 | Chinese Science Bulletin1997，42，17： | 2 |
| 5 | Shape separation of colloidal gold nanoparticles through salt-triggered selective precipitation显示文摘 | Guo, Zhirui Fan, Xu Xu, Lina Lu, Xiang Gu, Chunrong Bian, Zhiping Gu, Ning Zhang, Jinan Yang, Di | 2011 | 南京医科大学学报(自然科学版)2011，31，6： | 1 |
| 6 | Achieving high-purity colloidal gold nanoprisms and their application as biosensing platforms显示文摘 | Zhirui Guo Xu Fan Lianke Liu Zhiping Bian Chunrong Gu Yu Zhang Ning Gu Di Yang Jinan Zhang | 2010 | Journal of Colloid And Interface Science2010，，1： | 1 |
| 7 | The v-v energy transfer of highly vibrationally excitedstates (Ⅱ)──Vibrational quenching of CO(v) by H_2O显示文摘The vibrational energy transfer from highly vibrationally excited CO to H 2O molecules is studied by time-resolved Fourier transform infrared emission spectroscopy (TR FTIR). Following the 193 nm laser photolysis of CHBr 3 and O 2 the secondary reactions generate CO(v). The infrared emission of CO(v→v-1) is detected by TR FTIR. The excitation of H 2O molecules is not observed. By the method of the spectral simulation and the differential technique, 8 rate constants for CO(v)/H 2O system are obtained: (1.7±0.1), (3.4±0.2), (6.2±0.4), (8.0±1.0), (9.0±2.0), (12±3), (16±4) and (18±7) (10 -13cm 3·molecule -1·s -1). At least two reasons lead to the efficient energy transfer. One is the contributions of the rotational energy to the vibational energy defect and the other is the result of the complex collision. With the SSH and ab initio calculations, the quenching mechanism of CO(v) by H 2O is suggested. | WANG Baoshan+1, GU Yueshu 1*, LI Qiang+2 and KONG Fan’ao+2 1. Department of Chemistry, Shandong University, Jinan 250100, China 2. State Key Laboratory of Molecular Reaction Dynamics, Institute of Chemistry, Beijing 100080, China. * Corresponding aut | 1998 | Chinese Science Bulletin1998，43，19： | 0 |
| 8 | Ab Initio Study of the Oxygen Atom Abstracting Hydrogen from Difluoromethane and Dichloromethane显示文摘The three potential surfaces for reactions of the O atom to abstract H atoms from CH2F2and CH2Cl2 were studied using the ab initio method. The frequencies, geometries and energies ofall species are calculated. The best estimates of the heat of reactions are 30.92 KJ/mol and 13.01KJ/mol, and, the best potential barrier heights for both reactions are calculated to be 74.50 KJ/moland 67.22 KJ/mol, respectively. the second-order rate coefficients calculated are 2.8721×10-21 and4.2522×10-20 cm3/molecule-s at 298 K, respectively. | Jian Hua ZHOU Jing Zhong GUO Xiao Ping ZHANG Hong Ming YIN Chuan Pu LIU Yue Shu GU(Department of Chemistry, Shandong University, Jinan, 250100) | 1998 | Chinese Chemical Letters1998，9，9： | 0 |
| 9 | Studies on the Kinetics of the FO+CO Reaction显示文摘Two competitive channels of the reaction of FO with CO were calculated by the TST-RRKM theory and the classical transition state theory, respectively. The results show that thedirect channel dominates the reaction over the entire studied temperature range and the addition-elimination channel has important contributions at low temperatures. The cited reaction does notinfluence the fluorine regeneration and the sink of CO in the stratosphere. | Bao Shan WANG Hua HOU Yue Shu GU(Department of Chemistry, Shandong University, Jinan 250100) | 1998 | Chinese Chemical Letters1998，9，9： | 0 |
| 10 | COMPUTER SIMULATION OF 3-DIMENSIONAL DYNAMIC ASSEMBLY PROCESS OF MECHANICAL ROTATIONAL BODY显示文摘COMPUTERSIMULATIONOF3DIMENSIONALDYNAMICASSEMBLYPROCESSOFMECHANICALROTATIONALBODYYangJichangLiJinbanGuJinanZhangZiyuJiangsuUn... | Yang, Jichang Li, Jinban Gu, Jinan Zhang, Ziyu | 1998 | Chinese Journal of Mechanical Engineering1998，11，3： | 0 |
| 11 | Theoretical Investigation for the Abstraction Reaction of H with (CH_3)_3GeH显示文摘ion reaction of H atom with (CH3)3GeH has been studied using ab initio molecular orbital theory. The kinetics calculation has been deduced using the canonical variational transition state theory (CVT) with the small-curvature tunneling correction method (SCT) over the temperature range of 200-3000 K. The CVT/SCT rate constants exhibit typical non-Arrhenius behavior, and a three-parameter rate-temperature formula has been fitted as follows: k=6.66×10-18T2.33 exp(-60.3/T) (in units of cm3 molecule-1 s-1). | Qing Zhu ZHANG, Chun Sheng WANG, Yue Shu GU* School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 Abstract: The | 2002 | Chinese Chemical Letters2002，13，12： | 0 |
| 12 | Theoretical Studies on the Abstraction Reaction of Atomic O(^3p) with Si2H6显示文摘The hydrogen abstraction reaction of O (3P) with Si2H6 has been studied theoretically.Two transition states of 3A' and 3A' symmetries have been located for this abstraction reaction.Geometries have been optimized at the UMP2 level with 6-311G+(d) basis set. G3MP2 has been used for the final single-point energy calculation. The rate constants have been calculated over a wide temperature range of 200~3000 K using canonical variational transition-state theory (CVT)with small curvature tunneling effect (SCT). The calculated CVT/SCT rate constants match well with the experimental value. | Qing Zhu ZHANG, Chun Sheng WANG, Shao Kun WANG, Yue Shu GU* School of Chemistry and chemical engineering, Shandong University, Jinan 250100 Abstract: The hydrogen | 2002 | Chinese Chemical Letters2002，13，7： | 0 |
| 13 | The v-v energy transfer of highly vibrationally excited states (I)──Vibrational quenching of CO(v) by CO_2显示文摘The relaxation of the highly vibrationally excited CO (v=1-8) by CO\-2 is studied by time_resolved Fourier transform infrared emission spectroscopy (TR FTIR). 193 nm laser photolysis of the mixture of CHBr\-3 with O\-2 generates the highly vibrationally excited CO(v) molecules. TR FTIR records the intense infrared emission of CO(v→v-1). The vibrational populations of each level of CO(v) have been determined by the method of spectral simulation. Based on the evolution of the time resolved populations and the differential method, 8 energy transfer rate constants of CO(v=1-8) to CO 2 molecules are obtained: (5.7±0.1), (5.9±0.1), (5.2±0.2), (3.4±0.2), (2.4±0.3), (2.2±0.4), (2.0±0.4) and (1.8±0.6) (10 -14 cm 3·molecule -1·s -1), respectively. A two_channel energy transfer model can explain the feature of the quenching of CO(v) by CO 2. For the lower vibrational states of CO, the vibrational energy transfers preferentially to the υ\-3 mode of CO 2. For the higher levels, the major quenching channel changes to the vibrational energy exchange between CO(v→v-1) and the υ\-1 mode of CO 2. | WANG Baoshan\+1, GU Yueshu\+ 1*, HE Yong\+2 and KONG Fan’ao\+2 1. Department of Chemistry, Shandong University, Jinan 250100, China 2. State Key Laboratory of Molecular Reaction Dynamics, Institute of Chemistry, Beijing 100080, China. * Corresponding | 1998 | Chinese Science Bulletin1998，43，18： | 0 |
| 14 | A study of the quenching of PCl(b1∑+) by the E-V model显示文摘Using the reaction of Ar(3Po,2) + PCl3 as a source of PCl(b1 +), we have measured the quenching rate constants of PCl(b,v’=0) for more than 20 quenchers. The E-V model is used to calculate the rate constants. The comparison of the calculated values with the experimental ones shows that the quenching of PCl(b) by the reagent molecules results from the electronic to vi-brational energy near resonance transfer and has been related to the Franck-Condon factors for the PCl(b,v’=0-a,v'=m) transition and to the anharmonicities of the terminal bonds of the quenchers. In addition, the influence of the polarizability of the reagent molecule on the quenching rate constant is discussed. | LIU, Chuan-Pu ZOU, Sheng-Li GUO, Jing-Zhong GU, Yue-ShuDepartment of Chemistry, Shandong University, Jinan, Shandong 250100, ChinaCHU, Yan-Nan CAO, De-ZhaoLaser Spectroscopy Laboratory, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui 230031, China | 1997 | Chinese Journal of Chemistry1997，15，3： | 0 |
| 15 | Rapid Gas Chromatographic Assay of Diazinon:Application to the Study of Diazinon Kinetics in Rats显示文摘A rapid method was developed for the determination of diazinon in plasma using gas chromatography with electron-capture detection (GC-ECD). Following a single extraction with hexane from 100μl of plasma, diazinon was quantitated on a 3% OV-17 column. The detection limit was 10 ng/ml and linearity was obtained in the range of 25 ng/ml-2500 ng/ml.The applicability of the assay to single-dose kinetics in rats is | WU HONG-XING CL. EVREUX-GROS AND J. DESCOTES(Poisons Centre and Laboratory of Fundamental and Clinical Immunotoxicology, INSERM U80, Alexis Carrel Faculty of Medicine, 69008 Lyon, France Department of Public Health, Medical College, Jinan University, Gu | 1994 | Biomedical and Environmental Sciences1994，7，4： | 0 |
| 16 | Effect of CO (a^3Π )vibrational distribution on energy transfer reaction between CO (a^3Π )and NO (X^2Π_i )显示文摘THE metastable CO(a^3Ⅱ) molecule is known to be an important energy carrier. Its energytransfer reactions have been greatly interesting the dynamics workers. Previous studies of theenergy transfer reactions between CO(a^3Ⅱ) and NO(X^3Ⅱ_i) were mainly made in flow sys-tems. The state-to-state process in the energy transfer between them were seldomstudied. Yu et al. investigated the reaction of CO(a^3Ⅱ) with NO(X^3Ⅱ_i) under the condi- | LIU Chuanpu, LI Xiangyang, GUO Jingzhong, GU Yueshu, LI Xuechu, SHEN Guanlin and CHEN Hong1. Department of Chemistry, Shandong University, Jinan 250100, China 2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China | 1997 | Chinese Science Bulletin1997，42，13： | 0 |
| 17 | Ab initio Investigation of Decomposition of HCF_2OH显示文摘IntroductionDifluoromethanolhasbeenconsideredtobeofatmosphericimportancebecauseitistheproductofthereactionofO(1D)withCH2F2[1,... | HOU Hua, GUO Jing zhong ** , LIU Chuan pu, GU Yue shu and DENG Cong hao (Department of Chemistry, Shandong University, Jinan, 250100) | 1999 | Chemical Research in Chinese Universities1999，15，1： | 0 |
| 18 | Controlling molecular weight of naphthalenediimide-based polymer acceptor P(NDI2OD-T2)for high performance all-polymer solar cells显示文摘A widely-used naphthalenediimide(NDI) based electron acceptor P(NDI2OD-T2) with different numberaverage molecular weight(M_n) of 38(N2200_L), 56(N2200_M), 102(N2200_H) kD a were successfully prepared.The effect of molecular-weight on the performance of all-polymer solar cells based on Poly(5-(5-(4,8-bis(5-decylthiophen-2-yl)-6-methylbenzo[1,2-b:4,5-b']dithophen-2-yl)thiophen-2-yl)-6,7-difluoro-8-(5-methylthiophen-2-yl)-2,3-bis(3-(octyloxy)phenyl)quinoxaline)(P2F-DE):N2200 was systematically investigated. The results reveal that N2200 with increased Mn show enhanced intermolecular interactions, resulting in improved light absorption and electron mobility. However, the strong aggregation trend of N2200_H can cause unfavorable morphology for exciton dissociation and carrier transport. The blend film using N2200 with moderate Mn actually develops more ideal phase segregation for efficient charge separation and transport, leading to balanced electron/hole mobility and less carrier recombination. Consequently, all-polymer solar cells employing P2F-DE as the electron donor and N2200_M as the electron acceptor show the highest efficiency of 4.81%, outperforming those using N2200_L(3.07%)and N2200_H(3.92%). Thus, the Mn of the polymer acceptor plays an important role in all-polymer solar cells, which allows it to be an effective parameter for the adjustment of the device morphology and efficiency. | Yu Lei Jianxia Sun Jianyu Yuan Jinan Gu Guanqun Ding Wanli Ma | 2017 | Journal of Materials Science & Technology2017，33，5： | 0 |
| 19 | Photovoltaic devices employing ternary PbSxTe1-x nanocrystals显示文摘Colloidal quantum dots(CQDs), especially lead chalcogenide CQDs, are regarded as promising materials for the next generation solar cells, due to their large absorption coefficient, excellent charge transport,and multiple exciton generation effect. We successfully synthesized highly-crystalline, monodispersed,well-alloyed PbS_xTe_(1-x) nanocrystals via a one-pot, hot injection reaction method. Energy-filtered transmission electron microscopy suggested that the S and Te anions were uniformly distributed in the alloy nanoparticles. The photovoltaic performance of CQD solar cells based on ternary PbS_xTe_(1-x) was reported for the first time. The photovoltaic devices using PbS_xTe_(1-x) were more efficient than either the pure PbS or the PbTe based devices. In addition, the PbS_xTe_(1-x) based devices showed a significantly improved stability than that of the PbTe based devices. | Xiangxiang Zhu Zeke Liu Guozheng Shi Jinan Gu Weiwei Wang Wanli Ma | 2017 | Journal of Materials Science & Technology2017，33，5： | 0 |
| 20 | The Effect of the CO(a^3П) Vibrational Population on the CO(a^3П)+ NO(X^2П) Energy Transfer Reaction显示文摘TheEffectoftheCO(a~3П)VibrationalPopulationontheCO(a~3П)+NO(X~2П)EnergyTransferReactionTheEffectoftheCO(a~3П)VibrationalPopulat... | Chuan Pu LIU Xiang Yang LI Jing Zhong GUO Yue Shu GU (Department of Chemistry,Shandong University,Jinan 250100)Xue Chu LI Guan Lin SHEN Hong CHEN (State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of Chemical Physics CAS,P.O.Box 110, D | 1996 | Chinese Chemical Letters1996，7，9： | 0 |
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