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| 1 | Research Progress of Medicinal Secondary Metabolites and Gene Cloning of Dendrobium officinale显示文摘Dendrobium officinale is one of the most precious medicinal plants in China. Its main medicinal ingredients are its secondary metabolites. However,it has the characteristics of limited sources,low active ingredient content and high cost,limiting the use of D. officinale.Studying the network structure and rate-limiting steps of secondary metabolites of medicinal components of D. officinale,analyzing the secondary metabolic synthesis process,mastering the production rules of its medicinal components and carrying out gene cloning or biosynthesis,etc.are of great significance for the rational development and utilization of D. officinale resources. This paper briefly reviews the progress of the research on the secondary metabolites of D. officinale,including the detection and identification of metabolites and the identification and cloning of key metabolic enzymes. | Ye LIANG Caihua ZOU Jindu LI Shilong LI Tianzi LI | 2019 | Medicinal Plant2019，10，3： | 8 |
| 2 | The hybridization between peptide nucleic acid containing azobenzene and DNA labeled nanoparticle on chip surfaces studied by atomic force microscopy显示文摘Peptide nucleic acid containing azobenzene (N-PNA) was covalently bound on chip surfaces. Complementary DNA probes labeled by gold nanoparticles hybridizated with PNA on chip surfaces. The hybridization was successfully detected with AFM by the microscopy mothed. The influences of the position and the cis-trans isomerization of azobenzene unit on hybridization were investigated with AFM. | LI JinDu CHEN Miao ZHANG HaoBo LIU Sheng LIU JianXi | 2008 | Chinese Science Bulletin2008，53，19： | 1 |
| 3 | Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences显示文摘Hybridization of peptide nucleic acids probe containing azobenzene (NH2-TNT4, N-PNAs) with DNA was performed by covalently immobilizing of NH2-TNT4 in sequence on the 3-mercaptopropionic acid self-assembled monolayer modified gold electrode with the helps of N-(3-dimethylaminopropy1)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and the hybrid was coded as N-PNAs/DNA. Using [Fe(CN)6]4-/3- (1:1) as the electrochemical indicator, the electrochemical properties of the N-PNAs self-assembled monolayer (N-PNAs-SAMs) and N-PNAs/DNA hybridization system under the conditions of before and after UV light irradiation were characterized with cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectra (EIS). Results showed that the redox currents decreased with the increase of irradiation time, suggesting that the ability of the charge transfer on the electrode surface was weakened and the conformation of hybrid system had been changed, and the control of PNAs/DNA hybridization could be realized by UV light irradiation. | LIU Sheng CHEN Miao LI JinDu ZHANG HaoBo WANG JinQing YANG ShengRong | 2009 | Science China Chemistry2009，52，7： | 1 |
| 4 | Synthesis and photo-activity of peptide nucleic acids containing an azobenzene unit显示文摘We prepared the azobenzene PNA unit and synthesized various PNAs containing an azobenzene unit by a typical tBoc method. Both the azobenzene PNA unit and PNA containing an azobenzene unit (N-PNA) showed reversible photoisomerization with UV and visible light irradiation. The hybridization of N-PNA with complementary DNA resulted in a considerable decrease of the photoisomerization rate constant of the azobenzene. | YUE ShanShan LI JinDu ZHANG JunYan LU JinJun CHEN Miao | 2009 | Chinese Science Bulletin2009，54，24： | 0 |
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