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| 1 | The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells显示文摘The herbal medicine Tong Luo Jiu Nao(TLJN)contains geniposide(GP)and ginsenoside Rg1 at a molar ratio of 10:1.Rg1 is the major component of another herbal medicine,panax notoginseng saponin(PNS).TLJN has been shown to strengthen brain function in humans,and in animals it improves learning and memory.We have previously shown that TLJN reduces amyloidogenic processing in Alzheimer’s disease(AD)mouse models.Together this suggests TLJN may be a potential treatment for patients with dementia.Because chronic damage of the central nervous system by formaldehyde(FA)has been presented as a risk factor for age-associated cognitive dysfunction,in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA.FA-exposed murine N2a neuroblastoma cells were incubated with TLJN,its main ingredient GP,as well as PNS,to measure cell viability and morphology,the rate of apoptosis and expression of genes encoding Akt,FOXO3,Bcl2 and p53.The CCK-8 assay,cytoskeletal staining and flow cytometry were used to test cell viability,morphology and apoptosis,respectively.Fluorescent quantitative real-time PCR(qRT-PCR)was used to monitor changes in gene expression,and HPLC to determine the rate of FA clearance.Treatment of N2a cells with 0.09 mmol L?1 FA for 24 h significantly reduced cell viability,changed cell morphology and promoted apoptosis.Both TLJN and GP conferred neuroprotection to FA-treated N2a cells,whereas PNS,which had to be used at lower concentrations because of its toxicity,did not.Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient,GP,has a major role in this efficacy.This presents purified GP as a drug or lead compound for the treatment of AD. | CHEN JinYan SUN MengRu WANG XingHua LU Jing WEI Yan TAN Yan LIU Ying GTZ Jürgen HE RongQiao HUA Qian | 2014 | Science China(Life Sciences)2014，57，4： | 15 |
| 2 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 3 | A cluster-based power-efficient MAC protocol for event-driven sensing applications显示文摘 | LAZAROU G Y LI Jing PICONE J | 2007 | Ad hoc Networks2007，5，7： | 1 |
| 4 | Towards a structure-function analysis of bovine lactoferricin and related tryptophan and argini-necontaining peptides 显示文摘 | Vogel H J Schibli D J Jing W G | 2002 | Biochemistry and Cell Biology2002，80，1： | 1 |
| 5 | On the nonlinear dynamic behavior of a rotor-bearing system显示文摘 | JING J P MENG G SUN Y | 2004 | Journal of Sound and Vibration2004，274，35： | 1 |
| 6 | An effective continuum damage mechanics model for creep-fatigue life assessment of a steam turbine rotor显示文摘 | Jing J P Meng G Sun Y | 2003 | International Journal of Pressure Vessels and Piping2003，80，6： | 1 |
| 7 | Thioredoxin-interacting protein regulates insulin transcription through microRNA-204显示文摘 | Xu G Chen J Jing G | 2013 | Nature Medicine2013，19，9： | 1 |
| 8 | Policy burdens, firm performance, and management turnover 显示文摘 | Liao G Chen X Jing X Stin J | 2009 | China Economic Review2009，20，1： | 1 |
| 9 | Effects of Strength Mis-matching on the Fracture Behavior of Nuclear Pressure Steel A508-III Welded Joint显示文摘 | ZHU Z Q JING H Y GEA J G | 2005 | Materials Science and Engineering2005，，390： | 1 |
| 10 | On the non-linear dynamic behavior of a rotor-bearing system显示文摘 | Jing J P Meng G Sun Y | | 0，，3： | 1 |
| 11 | Influence of rapid solidification on microstructure, thermodynamic characteristic and the mechanical properties of solder/Cu joints of Sn-9Zn alloy显示文摘 | Jing Y X Sheng G M Zhao G J | 2013 | Materials & Design2013，52，： | 1 |
| 12 | A fast clustering-based feature subset selection algorithm for high-dimensional data 显示文摘 | Qin B S Jing J N Wang G T | 2013 | IEEE Transactions on Knowledge and Data Engineering2013，25，1： | 1 |
| 13 | An effective continuum damage mechanics model for creep-fatigue life assessment of a steam tur- bine rotor显示文摘 | Jing J P Meng G Sun Y | 2003 | International Journal of Pressure Vessels and Pip- ing2003，80，6： | 1 |
| 14 | 显示文摘 | Wang Y Z Yang Q B Shan G Wang C Du J Wang S Li Y Chen X Jing X Wei Y | 2005 | Materials Letters2005，59，： | 1 |
| 15 | Genome-Wide Identifi- cation of SNPs in MicroRNA Genes and the SNP Effects on MicroRNA Target Binding and Biogenesis显示文摘 | Jing G J Tong Y Zhang H M | 2012 | Human Muta- tion2012，33，： | 1 |
| 16 | Spectrophotometric Determination of Micro Amounts of Cadmium in WasteWater with Cadion and Triton X-100显示文摘 | Hsu C G Hu C S Jing J H | 1980 | Talanta1980，27，7： | 1 |
| 17 | Towards a structurefunction analysis of bovine lacoferricin and related tryptophan and argiminecontaining pepficlcs显示文摘 | VOGEL H J SCHIBLE D J JING W G | 2002 | Biochem Cell Biol2002，80，1： | 1 |
| 18 | Suppression of Magnaportheoryzae by culture filtrates of Streptomyces globisporus JK-1 显示文摘 | HUANG J LI G JING D | 2011 | Bi-ological Control2011，58，2： | 1 |
| 19 | Amorphous silicon pixel layers with cesium iodide converters for medical radiography显示文摘 | Jing T Goodman G A Drewery J | 1994 | IEEE Transactions on Nuclear Science1994，41，4： | 1 |
| 20 | Ag-graphcne hybrid conductive ink for writing electronics显示文摘 | XU L YANG G Y JING H Y WEI J HAN Y D | 2014 | Nanotechnology2014，25，05： | 1 |
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