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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam (Ruditapes philippinarum) in China显示文摘The amplified fragment length polymorphism (AFLP) technology was used to analyze the genetic diversities in four natural populations of Manila clam (Ruditapes philippinarum), distributed in four sea areas of China, i.e., the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea. Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity. The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon’s information index was from 0.256 8 to 0.275 6. By analyzing molecular variance (AMOVA), it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas. Cluster analysis by Nei’s pairwise distances grouped specimens by geographical origin, except the population of Qingdao. A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations. The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao. | LIU Xiangquan BAO Zhenmin HU Jingjie WANG Shi ZHAN Aibin LIU Hui FANG Jianguang WANG Rucai | 2007 | Acta Oceanologica Sinica2007，26，1： | 18 |
| 3 | Assessing the impacts of climate variability and human activities on streamflow in the water' source area of Baiyangdian Lake显示文摘作为在北方中国平原(NCP ) 的最大的沼泽地,拜伊昂达恩·莱克在维持水平衡和 NCP 的生态的健康起一个重要作用。在过去几十年,在与气候可变性和人的活动联系的 Baiyangdian 盆的减少的流速及流水量引起了一系列水和 eco 环境的问题。在这研究,我们确定了水里的流速及流水量上的气候可变性和人的活动的影响采购拜伊昂达恩·莱克的区域,基于从 1960 ~ 2008 的上面的 Tanghe 河集水(Baiyangdian 盆的亚盆) 的 hydrologic 变化的分析。气候弹性方法和水文学建模方法被用来区分气候可变性和人的活动的效果。结果证明年度流速及流水量显著地减少了(P > 0.05 ) 在 1.7, mm/a 和一个突然的变化在一年 1980 附近被识别。quantification 结果显示气候变化说明了减少的流速及流水量的 38%40% ,当人的活动说明了 60%62% 时。因此,人的活动的效果在拜伊昂达恩·莱克的水来源区域在流速及流水量的衰落上起了一个主导的作用。把生态系统作为拜伊昂达恩·莱克的健康,我们建议最小的生态的水需求和综合分水岭管理以后应该被保证。 | HU Shanshan LIU Changming ZHENG Hongxing WANG Zhonggen YU Jingjie | 2012 | Journal of Geographical Sciences2012，22，5： | 15 |
| 4 | Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri显示文摘The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization (FISH) with 18S—28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentric chromosome 10. After silver-staining, nucleolus organizer regions (NORs) could be observed on the telomere of the short arm of chromosome 10. However, one metaphase spread displayed an additional silver spot on the short arm of subtelocentric chromosome 12. | HUANG Xiaoting BAO Zhenmin BI Ke HU Jingjie ZHANG Can ZHANG Quanqi HU Xiaoli | 2006 | Acta Oceanologica Sinica2006，25，3： | 10 |
| 5 | Existence of Microsatellites in Expressed Sequence Tags of Common Carp (Cyprinus carpio L.) Available in GenBank dbEST Database显示文摘表示顺序标注的普通鲤鱼(EST ) 为存在 ofmicrosatellites,或简单顺序重复(SSR ) 被分析。在 NCBI dbEST 数据库, 10612sequences 的一个总数在 2004 年 12 月 31 日前被登记。2-6 nucleotidemicrosatellites 的完全的搜索导致了 513 包含 SSR EST 的鉴定,为 4.8% 总数的财务。聚合分析显示包含 SSR EST 的 73 个序列掉进 27 个组并且留下 440 EST 是独立的。467 唯一的包含 SSR EST 的一个总数被识别。这些 EST-SSRs 包含了许多简单顺序类型,并且 di-nucleotiderepeats 和 tri-nucleotiderepeats 是最丰富的,为 42.1% 全部和 27.9% 的财务分别地。thedinucleotide 重复, CA/TG 是最丰富的,由 GA/TC 列在后面。BLASTx 搜索证明 SSR loci 的 that38.1% 能与基因或蛋白质被联系知道或包含 SSR EST 的未知 function.BLASTx 搜索也在 zebrafishsequences 上显示出点击的高频率(98/179 ) 。 | HU Jingjie WANG Xiaolong HU Xiaoli BAO Zhenmin | 2006 | Journal of Ocean University of China2006，5，1： | 8 |
| 6 | In Silico Screening for Microsatellite Markers from Expressed Sequence Tags of Porphyra yezoensis (Bangiales, Rhodophyta)显示文摘The genomic resources of Porphyra yezoensis expressed sequence tags (ESTs) were utilized to identify simple sequence repeats (SSRs), or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high polymorphism. The microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis, 1 162 out of 21 954 ESTs contained microsatellites and cluster analysis indi- cated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons. A total of 290 unique SSR-containing genes were identified. The AAC SSRs were the most populous type of microsatellites. GC-rich microsatellites were predominant among all the microsatellites. | WANG Mengqiang HU Jingjie ZHUANG Yunyun ZHANG Lei LILT Wei MAO Yunxiang | 2007 | Journal of Ocean University of China2007，6，2： | 8 |
| 7 | Molecular identification of scallop planktonic larvae using species-specific microsatellites显示文摘The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However,the larvae identification by morphological characteristics is notoriously difficult,mainly due to the small size (usually being less than 150 μm) and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species,the Zhikong scallop Chlamys farreri,the noble scallop C. nobilis,the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers,which was specified by transferability analyses,assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1%~2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple,inexpensive and robust in identification analyses,and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years. | ZHAN Aibin HU Xiaoli BAO Lisui LU Wei PENG Wei WANG Mingling HU Jingjie | 2008 | Acta Oceanologica Sinica2008，27，5： | 3 |
| 8 | Fine‑mapping and association analysis of candidate genes for papilla number in sea cucumber,Apostichopus japonicus显示文摘The papilla number is one of the most economically important traits of sea cucumber in the China marketing trade.However,the genetic basis for papilla number diversity in holothurians is still scarce.In the present study,we conducted genomewide association studies(GWAS)for the trait papilla number of sea cucumbers utilizing a set of 400,186 high-quality SNPs derived from 200 sea cucumbers.Two significant trait-associated SNPs that passed Bonferroni correction(P<1.25E−7)were located in the intergenic region near PATS1 and the genic region of EIF4G,which were reported to play a pivotal role in cell growth and proliferation.The fine-mapping regions around the top two lead SNPs provided precise causative loci/genes related to papilla formation and cellular activity,including PPP2R3C,GBP1,and BCAS3.Potential SNPs with P<1E−4 were acquired for the following GO and KEGG enrichment analysis.Moreover,the two lead SNPs were verified in another population of sea cucumber,and the expressive detection of three potential candidate genes PATS1,PPP2R3C,and EIF4G that near or cover the two lead SNPs was conducted in papilla tissue of TG(Top papilla number group)and BG(Bottom papilla number group)by qRT-PCR.We found the significantly higher expression profile of PATS1(3.34-fold),PPP2R3C(4.90-fold),and EIF4G(4.23-fold)in TG,implying their potential function in papilla polymorphism.The present results provide valuable information to decipher the phenotype differences of the papilla trait and will provide a scientific basis for selective breeding in sea cucumbers. | Xinghai Zhu Ping Ni Marc Sturrock Yangfan Wang Jun Ding Yaqing Chang Jingjie Hu Zhenmin Bao | 2022 | Marine Life Science & Technology2022，4，3： | 2 |
| 9 | An Efficient Procedure for Isolating Microsatellite DNAs from Sea Cucumber(Apostichopus japonicus)显示文摘The construction of enrichment library proves to be one of the efficient approaches for isolating microsatellites in this study. The genomic DNA of sea cucumber was digested with HaeIII and size-selected DNA fragments (250-700 bp) were ligated to an adaptor. Microsatellite-containing sequences were captured by using a combination of GA and CA probes, which were attached to a nylon membrane. The microsatellite enrichment library constructed in this study consisted of approximately 700 clones. Two hun-dred and thirty-two clones reacted positively after the library screening procedure. Of the 50 clones sequenced, all contained at least one microsatellite and one duplicate clone was found. Approximately 86% of the sequenced fragments permitted to design primers for sequence tagged microsatellite site (STMS). | HU Jingjie, ZHAN Aibin, LU Wei, HU Xiaoli, and BAO Zhenmin* Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Biotechnology, Ocean University of China, Qingdao 266003, P. R. China | 2007 | Journal of Ocean University of China2007，6，1： | 2 |
| 10 | Genetic diversity and specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C.farreri显示文摘The AFLP (amplified fragment length polymorphism)technique was used to analyse the genetic diversity in four scallop species,Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and C.farreri. The genetic similarity indexes of these four species are0.841 5, 0.786 3, 0.719 0 and 0.673 1, while Shannon diversity indexes are 43.52, 58.87, 80.16 and 92.83, respectively. As analyzed,the genetic diversities in two native species, i.e., C.farreri and C. nobilis, are higher than those in other two introduced species, A.irradians and P. yessoensis. The results also showed that C. nobilis and C. farreri shared the most common loci. The genetic distanceindicated that C. nobilis and C. farreri are closely related. Moreover, out of 510 AFLP markers, 21 specific bands are found todistinguish the four species scallops and these markers may be applied to the specific germplasm characterization and molecularassistant classification in scallops. | CHEN Shengping BAO Zhenmin PAN Jie HU Jingjie | 2005 | Acta Oceanologica Sinica2005，24，4： | 2 |
| 11 | Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining显示文摘The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification. | ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie | 2008 | Journal of Ocean University of China2008，7，2： | 2 |
| 12 | Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding显示文摘Cytogenetic analysis was performed for the bay scallop(Argopecten irradians Lamarck 1819) and the Japanese scallop(Patinopecten yessoensis Jay 1857) by primed in situ labeling(PRINS) and propidium iodide(PI) banding techniques.The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A.irradians and on two submetacentric pairs in P.yessoensis.The histone H3 gene sites differed in number and location between these two species.The C-band-like patterns revealed by PI staining varied considerably between these two species.A.irradians displayed terminal bands at long arms on all chromosomes,centromeric bands on some pairs and interstitial bands on five pairs.P.yessoensis exhibited only centromeric bands on all chromosomes.These results would contribute to the better understanding of karyotype evolution in A.irradians and P.yessoensis. | ZHANG Lingling BAO Zhenmin WANG Jue WANG Shi HUANG Xiaoting HU Xiaoli HU Jingjie | 2007 | Acta Oceanologica Sinica2007，26，5： | 2 |
| 13 | Application of Live Monocells from Macroalgae to Shellfish Seed Production显示文摘 | Jixun Dai Hai Wang Baoqin Han Jingjie Hu Zhenmin Bao | 2000 | Marine Biotechnology2000，，1： | 1 |
| 14 | Mapping of ribosomal DNA and (TTAGGG)n telomeric sequence by FISH in the bivalve Patinopecten yessoensis (Jay,1857) 显示文摘 | Huang Xiaoting Hu Xiaoli Hu Jingjie | 2007 | Journal of Molluscan Studies2007，73，： | 1 |
| 15 | Methods Comparison for Microsatellite Marker Development:Different Isolation Methods,Different Yield Efficiency显示文摘Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. | ZHAN Aibin BAO Zhenmin HU Xiaoli LU Wei HU Jingjie | 2009 | Journal of Ocean University of China2009，8，2： | 1 |
| 16 | Cytoge- Argopecten techniques and fluorescence in situ hybridization 显示文摘 | Huang Xiaoting Hu Jingjie Hu Xiaoli et netic characterization of the bay scallop irradians irradians by multiple staining al | 2007 | Genes & Genetic Systems2007，82，： | 1 |
| 17 | En- ergy for Sustainable Road Transportation in China: Challenges, Initiatives and Policy hnplications 显示文摘 | HU Xiaojun CHANG Shiyan LI Jingjie | 2010 | Energy2010，35，11： | 1 |
| 18 | Chromosome rearrangements in Pectinidae (Bivalvia: Pteriomorphia) implied based on chromosomal localization of histone H3 gene in four scallops显示文摘 | Lingling Zhang Zhenmin Bao Shi Wang Xiaoting Huang Jingjie Hu | 2007 | Genetica2007，，2： | 1 |
| 19 | Genetic analysis of sweetpotato and wild relatives using inter-simple sequence repeats(ISSRs)显示文摘 | Jingjie Hu Makoto Nakatani Antonio Garcia Lalusin | 2003 | Breeding Science2003，53，： | 1 |
| 20 | New microsatellite markers developed from reported Ipomoea trifida sequences and their application to sweetpotato and its related wild species显示文摘 | Jingjie Hu Makoto Nakatani Antonio Garcia Lalusin Tatsuhito Fujimura | 2004 | Scientia Horticulturase2004，102，： | 1 |
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