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| 1 | Effect of nanopore size on poly(dT)_(30) translocation through silicon nitride membrane显示文摘The nanopore size effect on translocation of poly(dT)30through Si3N4 membrane is investigated.In this paper,we report that the speed of the poly(dT)30 transport through Si3N4 nanopores can be slowed down by half through increasing the nanopore diameter from 4.8 nm to 10.8 nm.The results are consistent with our simulation results.Besides,the current blockage induced by DNA passing through the nanopore is less obvious as pore diameter is larger,which is in good agreement with the theoretical prediction.The conclusion about DNA transport through nanopores is beneficial for the design of DNA sequencing devices. | SI Wei SHA JingJie LIU Lei QIU YingHua CHEN YunFei | 2013 | Science China(Technological Sciences)2013，56，10： | 9 |
| 2 | Integrated solid-state nanopore devices for third generation DNA sequencing显示文摘Third generation DNA sequencing relies on monitoring the ionic current blockage during the DNA molecule’s threading through a nanoscale pore.It is still really tough to attain the single base discrimination on a DNA strand by merely analyzing the ionic current due to speedy DNA translocation and low spatial resolution.More integrated configurations are pursued to present versatile comparative dissimilarities of the four bases by enhancing the spatial resolution within a DNA molecule translocation event,such as transverse tunneling current,local potential change,and capacitance oriented voltage resonance.In this mini review,the insight is provided into the status quo on several functionalized techniques and methodologies for DNA sequencing and furthermore concluding remark and outlook are presented. | WU GenSheng ZHANG Yin SI Wei SHA JingJie LIU Lei CHEN YunFei | 2014 | Science China(Technological Sciences)2014，57，10： | 4 |
| 3 | DNA sequencing technology based on nanopore sensors by theoretical calculations and simulations显示文摘DNA sequencing based on nanopore sensors is a promising tool for third-generation sequencing technology because of its special properties,such as revolutionized speed and low cost.With about two decades of nanopore technology development,the pioneering work has demonstrated the ability of nanopores to perform single-molecule detection and DNA sequencing.However,the microscopic mechanisms of DNA transport dynamics through nanopores remain largely unknown.Currently,DNA microscopic transport in a nanopore is difficult to characterize and several unexpected experimental observations are equivocal.This limitation can be resolved using theoretical calculations and simulations.These computational methods can monitor the entire dynamic process that DNA undergoes in solution at a single-atom resolution that can accurately unveil the mystery of DNA transport dynamics and predict certain unexpected phenomena.This paper mainly reports the recent applications of computational and simulation methods applied to the study of DNA transport through both biological and synthetic nanopores.We hope the theoretical calculations and simulations of DNA transport through nanopores can benefit the design of DNA sequencing devices. | Wei Si Yin Zhang Gensheng Wu Jingjie Sha Lei Liu Yunfei Chen | 2014 | Chinese Science Bulletin2014，59，35： | 3 |
| 4 | Temperature effect on translocation speed and capture rate of nanopore-based DNA detection显示文摘We study the effects of electrolyte temperature on DNA molecule translocation experimentally without and with a temperature gradient across nanopore membranes.The same temperatures on both electrolyte chambers are first considered.The DNA molecule translocation time is measured to be 2.44 ms at 2°C in both chambers,which is 1.57 times longer than at 20°C.Then the temperature difference effect is characterized in both chambers.The results show that the DNA translocation speed can be slowed down as long as one side temperature is lowered,irrespective of the temperature gradient direction.This indicates that the thermophoretic driving force generated by a temperature gradient has no obvious effect on the threading speed of DNA molecules,while the main reason for the slowed DNA translocation speed is the increased viscosity.Interestingly,the capture rate of DNA molecules is enhanced under a temperature gradient condition,and the capture rate during DNA translocation from hot side at 21°C to cold one at 2°C is 1.7 times larger than that under the condition of both chambers at 20°C.Finally,an optimized configuration is proposed to acquire higher capture rates and lower DNA translocation speeds. | ZHANGYin WU GenSheng MA Jian YUAN ZhiShan SI Wei LIU Lei SHA JingJie CHEN YunFei | 2015 | Science China(Technological Sciences)2015，58，3： | 2 |
| 5 | Glass capillary nanopore for single molecule detection显示文摘Single molecule detection based on nanopore technology is a very promising approach for medical diagnostics, drug therapy and even DNA sequencing. Compared with other biological nanopores and solid-state nanopores, the glass capillary nanopore has low cost, easy availability and stable mechanical characteristics, thus it has been widely used in the nanopore technology for single molecule detection. In this review, we will focus on the studies of the glass nanopore sensors. The popular glass nanopore fabrication methods would be introduced, and the applications of glass nanopores in the detection of nanoparticles, proteins and DNA molecules would be presented. We hope this review will help widen field of vision and promote the development of the nanopore technology based on the glass capillary nanopores. | SHA JingJie SI Wei XU Wei ZOU YiRen CHEN YunFei | 2015 | Science China(Technological Sciences)2015，58，5： | 2 |
| 6 | Retarding and manipulating of DNA molecules translocation through nanopores显示文摘Nanopores are emerging sensitive sensors that can detect and analyze single charged molecule.Nanopores present a promising approach for sequencing human genome below US$1,000 because of its superior performance,such as high throughput and low cost.However,a dominant bottleneck,that is,the high translocation speed of DNA molecules,has to be overcome.This property decreases accuracy of nanopore sensors to the single-base level.In this review,we mainly introduce the recent research works of retarding and manipulating of DNA motion through nanopores by actively control of three forces,which are the driving force,interaction force between nanopore and molecule,and exterior drag force.Lastly,conclusion and further outlook are presented on future directions of nanopore-based DNA sequencing technology. | Yin Zhang Gensheng Wu Wei Si Jingjie Sha Lei Liu Yunfei Chen | 2014 | Chinese Science Bulletin2014，59，35： | 2 |
| 7 | Investigation on the interaction length and access resistance of a nanopore with an atomic force microscopy显示文摘Nanopore devices have attracted a lot of attention for their potential application in DNA sequencing. Here, we study how an occluding object placed near a nanopore affects its access resistance by integrating an atomic force microscopy with a nanopore sensor. It is found that there exists a critical hemisphere around the nanopore, inside which the tip of an atomic force microscopy will affect the ionic current. The radius of this hemisphere, which is a bit smaller than the theoretical capture radius of ions, increases linearly with the applied bias voltage and quadratically with the nanopore diameter, but is independent of the operation modes and scanning speeds of the atomic force microscopy. A theoretical model is also proposed to describe how the tip position and geometrical parameters affect the access resistance. | SI Wei YANG HaoJie LI Kun WU GenSheng ZHANG Yin KAN YaJing XIE Xiao SHA JingJie LIU Lei CHEN YunFei | 2017 | Science China(Technological Sciences)2017，60，4： | 0 |
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