|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2显示文摘新兴的证据与肿瘤开发和前进显示出异常地表示的 microRNAs (miRNAs ) 的协会。然而,很少在胃的 carcinogenesis 对 miRNAs 的潜在的角色被知道。这里,我们执行了 miRNA microarray 屏蔽在配对的胃的癌症和他们的邻近的 nontumor 纸巾表示并且发现 miR-375 是的 miRNAs 差别极大地在胃的癌症纸巾的 downregulated。量的即时 PCR 分析证实那 miR-375 表情显著地从经历胃的切除术的病人与他们的 nontumor 对应物相比在超过 90% 主要胃的癌症被减少。miR-375 的 Overexpression 显著地在 vitro 并且在 vivo 禁止了胃的癌症房间增长。在胃的癌症房间的 miR-375 的强迫的表示显著地减少了 Janus kinase 的蛋白质水平 2 ( JAK2 )并且镇压带 JAK2 的 3 鈥? untranslated 区域的一个酶记者的活动,被预言的 miR-375-binding 地点的变化废除,显示那 JAK2 可以是 miR-375 目标基因。由由 RNAi 的 JAK2 的 AG490 或 silencing 的 JAK2 活动的任何一个抑制压制了类似于 miR-375 overexpression 的胃的癌症房间增长。而且, JAK2 的宫外的表示能部分颠倒 miR-375 引起的房间增长的抑制。最后,我们在胃的癌症发现了在 miR-375 表示和 JAK2 蛋白质水平之间的重要反的关联。因此,这些数据建议 miR-375 可以作为肿瘤 suppressor 工作由指向 JAK2 oncogene 潜在地调整胃的癌症房间增长,含有在胃的癌症的致病的 miR-375 的一个角色。 | Ling Ding Yanjun Xu Wei Zhang Yujie Deng Misi Si Ying Du Haomi Yao Xuyan Liu Yuehai Ke Jianmin Si Tianhua Zhou | 2010 | Cell Research2010，20，7： | 59 |
| 3 | Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin显示文摘LRP16 以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC ) 的雌激素和它的功能的效果的 LRP16 的应答的海角房间仍然是不清楚的。这里,我们证明 LRP16 基因的信使 rna 水平和倡导者活动被 17beta-estradiol (E2 ) 显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa 人 EC 房间。尽管 Ishikawa 细胞的生长率没被 LRP16 的宫外的表示显然影响, Transwell 试金的结果显示出 LRP16-overexpressing 细胞的侵略能力的近似 one-thirdincrease。由于分子的屏蔽,我们观察到 E-cadherin 的表示,与肿瘤转移联系的一个必要粘附分子,被 LRP16 镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那 LRP16 inhibitedE-cadherin transactivation。然而,抑制被雌激素剥夺废除,显示由 LRP16requires 的 E-cadherin 抄写的 down 规定嗯高山哈调停。染色质免疫降水分析表明到 E-cadherin 倡导者的 ERalpha 的绑定被 LRP16 反对,建议那 LRP16 能防碍嗯调停 alpha 的抄写。这些结果建议起来由雌激素的 LRP16 的规定能被在人的 EC 调整 E-cadherin 的 down 涉及侵略生长。 | Yuan Guang Meng Wei Dong Han Ya Li Zhao Ke Huang Yi Ling Si Zhi Qiang Wu Yi Ming Mu | 2007 | Cell Research2007，17，10： | 27 |
| 4 | Heat-shocked tumor cell lysate-pulsed dendritic cells induce effective anti-tumor immune response in vivo显示文摘瞄准:学习震惊热的肿瘤房间是否能提高肿瘤房间的效果在唤起反肿瘤免疫者反应的搏动 lysate 的树枝状的房间(DC ) 在活体内。方法:老鼠无差别的结肠癌房间(CT-26 ) 为 1 h 然后结冰融化在 42 度摄氏被加热。骨头导出髓的 DC 与震惊热的 CT-26 房间溶解产物(HSCT-26 DC ) 搏动了被招募使 syngeneic 免疫天真的 BALB/c 老鼠。肿瘤的细胞毒素的活动在老鼠怒气的特定的细胞毒素的 T 淋巴细胞(CTL ) 被 IFN-enzyme-linked 免疫点(ELISpot ) 和 LDH 版本试金评估。在老鼠结肠癌的 DC 建模的 HSCT-26 导致的免疫 prophylactic 效果与单个 CT-26 房间导致的那些相比肿瘤体积,腹转移和老鼠的生存时间上的搏动 lysate 的 DC (CT-26 DC ) 。结果:对待热的 CT-26 房间显示出更高的 hsp70 蛋白质表情。震惊热的 CT-26 房间溶解产物 pulsing 提高了骨头的 co-stimulatory 和 MHC-II 分子表示导出髓的 DC 以及 interleukin-12 p70 分泌物。HSCT-26 DC 导致的 IFN-gamma secreting CTL 被 CT-26 DC (P=0.002 ) 显著地多于那些导致。以前的 CTL 的特定的细胞毒素的活动在 10:1, 20:1,和 40:1 的连续 E/T 比率比后者 CTL 的家高。尽管他们之间没有统计差别,鼠标结肠癌模型比 CT-26 DC,种痘在肿瘤体积上组织证明种痘组织的 HSCT-26 DC 的肿瘤卷小(24 mm3 对 8 mm3, P=0.480 ) 。与 HSCT-26 DC 使免疫的老鼠的中部的生存时间比与 CT-26 DC 使免疫的那些的长(57 d 对 43 d, P=0.0384 ) 。结论:震惊热的肿瘤房间搏动 lysate 的 DC 能有效地唤起反肿瘤免疫者反应在活体内并且用作一支新奇基于 DC 的肿瘤疫苗。 | Jian Qiu Guo-Wei Li Yan-Fang Sui Hong-Ping Song Shao-Yan Si Wei Ge | 2006 | World Journal of Gastroenterology2006，12，3： | 20 |
| 5 | Infection with novel coronavirus(SARS-CoV-2)causes pneumonia in Rhesus macaques显示文摘The 2019 novel coronavirus(SARS-CoV-2)outbreak is a major challenge for public health.SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases,with a mortality rate of^6.4%worldwide(based on World Health Organization daily situation report).However,the dynamics of viral infection,replication and shedding are poorly understood.Here,we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2.After intratracheal inoculation,the first peak of viral RNA was observed in oropharyngeal swabs one day post infection(1 d.p.i.),mainly from the input of the inoculation,while the second peak occurred at 5 d.p.i.,which reflected on-site replication in the respiratory tract.Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals,characterized by hyperemia and edema,and infiltration of monocytes and lymphocytes in alveoli.We also identified SARS-CoV-2 RNA in respiratory tract tissues,including trachea,bronchus and lung;and viruses were also re-isolated from oropharyngeal swabs,bronchus and lung,respectively.Furthermore,we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2.The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics. | Chao Shan Yan-Feng Yao Xing-Lou Yang Yi-Wu Zhou Ge Gao Yun Peng Lian Yang Xue Hu Jin Xiong Ren-Di Jiang Hua-Jun Zhang Xiao-Xiao Gao Cheng Peng Juan Min Ying Chen Hao-Rui Si Jia Wu Peng Zhou Yan-Yi Wang Hong-Ping Wei Wei Pang Zheng-Fei Hu Long-Bao Lv Yong-Tang Zheng Zheng-Li Shi Zhi-Ming Yuan | 2020 | Cell Research2020，30，8： | 19 |
| 6 | Preventive and therapeutic effect of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage显示文摘Objective:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage.Methods:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group,model group and simvastatintreated group randomly with 20 rats in each group.Rats in the model group and simvastatintreated group were infused with autologous fresh uncoagulated blood to the right brain tissue of the basal ganglia to build the cerebral hemorrhage model,while rats in the control group were treated with the same amount of normal saline.Then,rats in the simvastatin-treated group were given a gavage of 3 mg/kg of simvastatin once a day after modeling.Rats in the three groups were given nerve dysfunction score(NDS) and wet-dry weighting method was used to detect the brain water content(BWC) of brain tissues around the lesion of the rats.Then Nissl staining was conducted and the undamaged neurons were counted.Immunohistochemical SP method was applied to count the number of NF-d the immuno fluorκB,TLR4 and IL-1escence method wasβ positive cells in brain tissues around the lesions,an employed to determine the expression levels of NF-κB,TLR4 and IL-1me points were aβ proteins.Results:The NDS results of the simvastatin-treated group at all till significantly higher than those of the model group(P < 0.05);the BWC values of the simvastatin-treated group at all time points were all significantly lower than those of the model group at the same periods(P < 0.05);the number of the undamaged neurons around the lesions of the simvastatin-treated group at all time points were all significantly higher than those of the model group(P < 0.05);seven days after treatment,the number of the NF-κB,TLR4 and IL-1β positive cells in brain tissues around the lesions of the simvastatin-treated group were all significantly lower than those of the model group(P < 0.05),and its expression levels of NF-ower than those of the model group(κB,TLR4 and IL-1P < 0.05).Conclusioβ protein were also significantly lns:Simvastatin can inhibit the expressions of NF-κB,TLR4 and IL-1β proteins in rats with cerebral hemorrhage,and protect neurons and reduce secondary inflammatory damages by down-regulating the above protein-mediated inflammatory responses. | Hong-Xia Zhou Ling-Huan Gao Ling-Li Meng Yu-Xin Zhang Zi-Feng Wei Dao-Wen Si | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，2： | 17 |
| 7 | Highly Stretchable,Elastic,and Sensitive MXene-Based Hydrogel for Flexible Strain and Pressure Sensors显示文摘Electronic skin is driving the next generation of cutting-edge wearable electronic products due to its good wearability and high accuracy of information acquisition.However,it remains a challenge to fulfill the requirements on detecting full-range human activities with existing flexible strain sensors.Herein,highly stretchable,sensitive,and multifunctional flexible strain sensors based on MXene-(Ti_(3)C_(2)T_(x)-)composited poly(vinyl alcohol)/polyvinyl pyrrolidone double-network hydrogels were prepared.The uniformly distributed hydrophilic MXene nanosheets formed a three-dimensional conductive network throughout the hydrogel,endowing the flexible sensor with high sensitivity.The strong interaction between the double-network hydrogel matrix and MXene greatly improved the mechanical properties of the hydrogels.The resulting nanocomposited hydrogels featured great tensile performance(2400%),toughness,and resilience.Particularly,the as-prepared flexible pressure sensor revealed ultrahigh sensitivity(10.75 kPa^(-1))with a wide response range(0-61.5 kPa),fast response(33.5 ms),and low limit of detection(0.87 Pa).Moreover,the hydrogel-based flexible sensors,with high sensitivity and durability,could be employed to monitor fullrange human motions and assembled into some aligned devices for subtle pressure detection,providing enormous potential in facial expression and phonation recognition,handwriting verification,healthy diagnosis,and wearable electronics. | Yao Lu Xinyu Qu Wen Zhao Yanfang Ren Weili Si Wenjun Wang Qian Wang Wei Huang Xiaochen Dong | 2020 | Research2020，，1： | 14 |
| 8 | Genesis of sandstone-type uranium deposits along the northern margin of the Ordos Basin,China显示文摘Sandstone-type U mineral resources are among the important sources for nuclear energy.The U deposits in the Ordos Basin in China form part of the northern segment of the sandstone-hosted Central Asian Uranium MegaProvince.Two types of mineralizations are recognized in this basin:'phreatic permeable type'and'interlayer permeable type',both exhibiting features equivalent to roll-front subtypes.The'interlayer permeable type'is widely accepted as the dominant mineralization type for sandstone-type uranium deposits within large-scale basins,also designated as the'interlayer oxidation zone type',based on the horizontal color zoning model representing changing redox conditions.Here we synthesize data from several drill holes within the Ordos Basin,which suggest that major Mesozoic tectonic movements controlled the evolution of the sedimentary system in the basin.These tectonic movements contributed to the formation of three angular unconformities and four parallel unconformities as inferred from the stratigraphic relationships.In addition,other features such as vertical color zoning,paleo-channel controlled tabular or lentoid ore bodies(without roll-type)and a group interlayer horizontal zoning of altered minerals are also documented.Sequence stratigraphic analysis indicates that the Ordos Basin generally witnessed four cycles of water level variations during Mesozoic.During the variations,three high water level and three low water level events were recorded.Biological characteristics imply that the Ordos Basin went through multiple arid to humid climatic evolutions during Mesozoic.Combining the newly documented features with some novel concepts on the hydrodynamic mechanism for supergene ore-forming fluids,we propose a metallogenic model which invokes the importance of tectonic movements and water level fluctuations to explain the genesis of uranium deposits along the northern margin of the Ordos Basin. | Ruoshi Jin Xueming Teng Xiaoguang Li Qinghong Si Wei Wang | 2020 | Geoscience Frontiers2020，11，1： | 13 |
| 9 | Patterns,magnitude,and controlling factors of hydraulic redistribution of soil water by Tamarix ramosissima roots显示文摘Tamarix spp.(Saltcedar)is a facultative phreatophyte that can tolerate drought when groundwater is not accessed.In addition to deep water uptake,hydraulic redistribution(HR)is another factor contributing to the drought tolerance of Tamarix spp.In this study,data on soil volumetric moisture content(θ),lateral root sap flow,and relevant climate variables were used to investigate the patterns,magnitude,and controlling factors of HR of soil water by roots of Tamarix ramosissima Ledeb.in an extremely arid land in Northwest China.Results showed evident diurnal fluctuations in θ at the depths of 30 and 50 cm,indicating 'hydraulic lift'(HL).θ increased remarkably at 10 and 140 cm but decreased at 30 and 50 cm and slightly changed at 80 cm after rainfall,suggesting a possible 'hydraulic descent'(HD).However,no direct evidence was observed in the negative flow of lateral roots,supporting HR(including HL and HD)of T.ramosissima.The HR pathway unlikely occurred via lateral roots;instead,HR possibly occurred through adventitious roots with a diameter of 2-5 mm and a length of 60-100 cm.HR at depths of 20-60 cm ranged from 0.01-1.77 mm/d with an average of 0.43 mm/d,which accounted for an average of 22% of the estimated seasonal total water depletion at 0-160 cm during the growing season.The climate factors,particularly vapor pressure deficit and soil water potential gradient,accounted for at least 33% and 45% of HR variations with depths and years,respectively.In summary,T.ramosissima can be added to the wide list of existing species involved in HR.High levels of HR may represent a considerable fraction of daily soil water depletion and substantially improve plant water status.HR could vary tremendously in terms of years and depths,and this variation could be attributed to climate factors and soil water potential gradient. | TengFei YU Qi FENG JianHua SI HaiYang XI Wei LI | 2013 | Journal of Arid Land2013，5，3： | 12 |
| 10 | Walnut-inspired microsized porous silicon/graphene core-shell composites for high-performance lithium-ion battery anodes显示文摘 | Wei Zhai Qing Ai Lina Chen Shiyuan Wei Deping Li Lin Zhang Pengchao Si Jinkui Feng Lijie Ci | 2017 | Nano Research2017，10，12： | 12 |
| 11 | Multiscale carbon foam confining single iron atoms for efficient electrocatalytic CO2 reduction to CO显示文摘Electrocatalytic CO2 reduction to CO is a sustainable process for energy conversion.However,this process is still hindered by the diffusi limited mass transfer,low electrical conductivity and catalytic activity.Therefore,new strategies for catalyst design should be adopted to solve these problems and improve the electrocatalytic performa nee for CO production.Herein,we report a multiscale carb on foam confining〔single iron atoms prepared with the assistant of S1O2 template.The pore-enriched environment at the macro-scale facilitates the diffusion of reacta nts and products.The graphe ne nano sheets at the nano-scale promote the charge tran sfer duri ng the reaction.The single iron atoms con fined in carb on matrix at the atomic-scale provide the active sites for electrocatalytic CO2 reductio n to CO.The optimized catalyst achieves a CO Faradaic efficiency of 94.9%at a moderate potential of-0.5 V vs.RHE.Furthermore,the performance can be maintained over 60 hours due to the stable single iron atoms coordi nated with four n itroge n atoms in the carb on matrix.This work provides a promising strategy to improve both the activity and stability of single atom catalysts for electrocatalytic CO2 reduction to CO. | Zheng Zhang Chao Ma Yunchuan Tu Rui Si Jie Wei Shuhong Zhang Zhen Wang Jian-Feng Li Ye Wang Dehui Deng | 2019 | Nano Research2019，12，9： | 11 |
| 12 | Recombinant adenovirus vector Ad-hIL-10 protects grafts from cold ischemia-reperfusion injury following orthotopic liver transplantation in rats显示文摘BACKGROUND:Interleukin 10(IL-10),a Th2 type cytokine,modulates inflammatory responses by inhibiting the production of proinflammatory cytokines.This study was designed to investigate the protective effects of adenovirus- mediated human IL-10(Ad-hIL-10)gene transfer on protecting grafts from cold ischemia-reperfusion injury following orthotopic liver transplantation in rats. METHODS:Adenoviruses encoding hIL-10 orβ-galactosidase (Ad-lacZ)were injected via the superior mesenteric vein into prospective donor animals.The donor liver was harvested 48 hours after transduction,and stored for 12 hours at 4℃ in lactated Ringer's solution prior to transplantation.The rats were divided into saline,Ad-lacZ,and Ad-hIL-10 groups.Liver function test,histopathological examination,reverse transcriptase-polymerase chain reaction(RT-PCR),and Western blotting were performed at 24 hours after transplantation in the three groups. RESULTS:Liver function(ALT and AST)was significantly improved,and the Suzuki score was significantly decreased in the Ad-hIL-10 group.The levels of hepatic TNF-α,MIP-2,ICAM-1 mRNA,and NF-κB protein in the Ad-hIL-10 group were significantly decreased.The expression of hIL-10 mRNA was detected by RT-PCR in Ad-hIL-10-treated grafts but not in controls treated with saline or Ad-lacZ. CONCLUSIONS:Donor pretreatment with Ad-hIL-10 down- regulates the expression of proinflammatory cytokines TNF-α,MIP-2,and ICAM-1 mRNA.hIL-10 protects against hepatic cold ischemia-reperfusion injury,at least in part,by suppressing NF-κB activation and subsequent expression of proinflammatory mediators. | Si, Zhong-Zhou Li, Jie-Qun Qi, Hai-Zhi He, Zhi-Jun Hu, Wei Li, Yi-Ning | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，2： | 11 |
| 13 | Identification and Fine Mapping of a Gene Related to Pale Green Leaf Phenotype near the Centromere Region in Rice(Oryza sativa)显示文摘A thermo-insensitive pale green leaf mutant (pgl2) was isolated from T-DNA inserted transgenic lines of rice (Oryza sativa L. subsp. japonica cv. Nipponbare). Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2 gene, an F2 population was constructed by crossing the mutant with Longtefu (Oryza sativa L. subsp. indica). The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll (Chl) content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres. | ZHU Li LiuWenzhen WU Chao LUAN Wei jiang Fu Ya ping Hu Guo cheng SI Hua min SUN Zong xiu | 2007 | Rice science2007，14，3： | 11 |
| 14 | First observation of tropospheric nitrogen dioxide from the Environmental Trace Gases Monitoring Instrument onboard the GaoFen-5 satellite显示文摘The Environmental Trace Gases Monitoring Instrument(EMI)is the first Chinese satellite-borne UV–Vis spectrometer aiming to measure the distribution of atmospheric trace gases on a global scale.The EMI instrument onboard the GaoFen-5 satellite was launched on 9 May 2018.In this paper,we present the tropospheric nitrogen dioxide(NO2)vertical column density(VCD)retrieval algorithm dedicated to EMI measurement.We report the first successful retrieval of tropospheric NO_(2) VCD from the EMI instrument.Our retrieval improved the original EMI NO_(2) prototype algorithm by modifying the settings of the spectral fit and air mass factor calculations to account for the on-orbit instrumental performance changes.The retrieved EMI NO_(2) VCDs generally show good spatiotemporal agreement with the satellite-borne Ozone Monitoring Instrument and TROPOspheric Monitoring Instrument(correlation coefficient R of ~0.9,bias<50%).A comparison with ground-based MAX-DOAS(Multi-Axis Differential Optical Absorption Spectroscopy)observations also shows good correlation with an R of 0.82.The results indicate that the EMI NO_(2) retrieval algorithm derives reliable and precise results,and this algorithm can feasibly produce stable operational products that can contribute to global air pollution monitoring. | Chengxin Zhang Cheng Liu Ka Lok Chan Qihou Hu Haoran Liu Bo Li Chengzhi Xing Wei Tan Haijin Zhou Fuqi Si Jianguo Liu | 2020 | Light(Science & Applications)2020，9，1： | 11 |
| 15 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 16 | Development of the personalized criteria for microscopic review following four different series of hematology analyzer in a Chinese large scale hospital显示文摘 | CUI Wei WU Wei WANG Xin WANG Geng HAO Ying-ying CHEN Yu LUO Dan SHOU Wei-ling ZHANG Shuo XIANG Xue-fang SI Yong-zhen CHEN Qian CAI Hao LI Tan SHEN Han SHANG Kun ZHANG Yong-qiang | 2010 | Chinese Medical Journal2010，，22： | 10 |
| 17 | Response of Populus euphratica Oliv.sap flow to environmental variables for a desert riparian forest in the Heihe River Basin,Northwest China显示文摘Being an important desert riparian forest in the lower reaches of the Heihe River Basin,Populus euphratica Oliv.forest functions as a natural barrier in maintaining and preserving the stability of local oases.Accordingly,accurately estimating the water use of P.euphratica is important for vegetation protection and water resource allocation.To date,little data are available for evaluating the hysteretic effects between sap flow and environmental variables,and for estimating the water use of desert riparian forest.In this study,we measured the sap flow velocity(V_s)of P.euphratica using the heat ratio method during the growing season of 2012.Based on the response of V_s to solar radiation(R_s)and vapor pressure deficit(VPD),we estimated the hourly Vsand daily V_s using the multivariable linear regression and a modified Jarvis-Stewart(JS)model,respectively.Hysteretic response of V_s to environmental variables was then evaluated using a sap flow model.We found the thresholds of V_s responses to R_s and VPD at both hourly and daily scales during the growing season,and successfully estimated the seasonal variations of hourly V_s and daily V_s using the JS model.At an hourly scale,the maximum V_s occurred earlier than the maximum VPD by approximately 0.5 h but later than the maximum R_s by approximate 1.0 h.At a daily scale,the maximum V_s lagged behind the maximum VPD by approximately 2.5 h while occurred earlier than the maximum R_s by approximately 2 h.However,hysteretic response of V_swas weakened when R_s and VPD were measured together using the JS model at both hourly and daily scales.Consequently,short-term and intensive field campaigns,where V_s and environmental variables can be measured,may be used to estimate short-run sap flow and stand transpiration using only two environmental variables. | LI Wei SI Jianhua YU Tengfei LI Xiaoyan | 2016 | Journal of Arid Land2016，8，4： | 9 |
| 18 | Effect of nanopore size on poly(dT)_(30) translocation through silicon nitride membrane显示文摘The nanopore size effect on translocation of poly(dT)30through Si3N4 membrane is investigated.In this paper,we report that the speed of the poly(dT)30 transport through Si3N4 nanopores can be slowed down by half through increasing the nanopore diameter from 4.8 nm to 10.8 nm.The results are consistent with our simulation results.Besides,the current blockage induced by DNA passing through the nanopore is less obvious as pore diameter is larger,which is in good agreement with the theoretical prediction.The conclusion about DNA transport through nanopores is beneficial for the design of DNA sequencing devices. | SI Wei SHA JingJie LIU Lei QIU YingHua CHEN YunFei | 2013 | Science China(Technological Sciences)2013，56，10： | 9 |
| 19 | Continuous Low-dose-rate Irradiation of Iodine-125 Seeds Inhibiting Perineural Invasion in Pancreatic Cancer显示文摘 | Zheng Lu Teng-Hui Dong Pei-Ren Si Wei Shen Yi-Liang Bi Min Min Xin Chen Yan Liu | 2016 | Chinese Medical Journal2016，，20： | 9 |
| 20 | Facile synthesis and characterization of novel pseudo-hypercrosslinked resin显示文摘一种 pseudo-hypercrosslinked 聚合物树脂第一经由连续 Friedel 被综合苯, diphenyl 和他们的 dichloromethyl 的手艺完化聚合衍生物。并且这些树脂的微形态学和吸附性质被调查。结果证明新奇树脂有高度特定的表面积(581.26 974.88 m2/g ) ,高毛孔的体积(0.56 1.65 mL/g ) ,小平均多孔的半径(1.93 3.67 nm ) 和为小非极的器官的分子的优秀吸附性质。 | Si Guo Yuan Shao Hui Zhang Wei Hua Zou Yong Hao Zhou Xiao Hui Zhou | 2008 | Chinese Chemical Letters2008，19，5： | 9 |
您所在的机构暂未开通此部分的功能，请联系管理员