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| 1 | Expert consensus on the glycemic management of critically ill patients显示文摘Introduction The incidence of hyperglycemia is 40-60%in critically ill patients and is up to 60-80%in those who have undergone car-diac surgery.[1]The results of an epidemiological study in the United States showed that 28.6%of diabetic patients and 9.3%of non-diabetic patients had elevated mean daily glucose on the day of ICU admission.[2]In critically ill patients,elevated blood glucose is primarily the result of stress,and stress-induced hy-perglycemia is an independent risk factor associated with prog-nosis,regardless of a previous diagnosis of diabetes.Nutritional therapy has become an integral treatment option for patients in the ICU,[3,4]though nearly 30%of patients with enteral nu-trition and 44-50%with parenteral nutrition(PN)experience elevated glucose.[5,6]Intensive insulin therapy(IIT)is an impor-tant treatment for controlling hyperglycemia in critically ill pa-tients,but it also carries a corresponding risk of hypoglycemia. | Zhixiong Wu Jiao Liu Dong Zhang Kai Kang Xiangrong Zuo Qianghong Xu Aijun Pan Wei Fang Fen Liu You Shang Haiyan Yin Juntao Hu Jinglun Liu Jiangquan Fu Wei Zhang Yuan Zong Min Shao Feng Zhao Mei Meng Yanfei Mao Yingchuan Li Dechang Chen | 2022 | Journal of Intensive Medicine2022，2，3： | 9 |
| 2 | Efficient transfer and expression of human clotting factor IX cDNA in neonatal hemophilia B mice mediated by VSV-G pseudotyped retrovi-rus显示文摘The feasibility of in vivo gene therapy for hemophilia B by VSV-G pseudotyped retroviral vector was introduced. The novel packaging cell line 293GPG was used to produce VSV-G/G1NaBAIX pseudotyped virus with the highest liters up to 8.5×108cfu .mL-1. In contrast to the conventional retrovirus, VSV-G pseudotyped virus was more resistant to inactivation by serum complements (P<0.001). Our results also demonstrated that VSV-G pseudotyped virus was more stable in neonatal mice serum than in adult mice serum (P<0.01). After intraperitoneal injection of different doses of virus, hFIX antigen was detected and lasted for more than 120 d, the highest level reached (72.5+6.1) ng- mL11. Moreover, the functional activity was improved to some extent in all hFIX-treated mice, the most remarkable improvement was observed in the mice treated with higher dose of virus whose clotting activity increased to (3.4±1.5)% and APTT (activated partial thromboplastin time) reduced to (43.2±7.2) s. The anti-hFIX antibody was | WANG Hongwei YE Chenbo CHEN Li WANG Xuefeng QIU Xinfang XUE Jinglun LU Daru | 2001 | Chinese Science Bulletin2001，46，18： | 2 |
| 3 | Building of hFⅨ transgenic mice by spermatogenic cells显示文摘Human FⅨ expression vector pCMVⅨ was packaged by EffecteneTM reagent and injected into mice seminiferous tubules with glass pipettes. The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies. There were 2 (4%) mice being integrated with hFⅨ gene into chromosomes. 4.6 ng/mL of hFⅨ protein was expressed in plasma of one mouse, which was tested by ELISA. We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method. Meanwhile, it has also been proved to be an alternative choice for mammary gland bioreactor. | WANG Ning, CHEN Xiaoguang, CHEN Li, YAO Jihua, CHEN Haoming, SHEN Qi & XUE Jinglun State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China Correspondence should be addressed to Xue Jinglun (e-mail: jlxue @fudan.ac.cn) | 2003 | Chinese Science Bulletin2003，48，19： | 2 |
| 4 | Adeno-associated virus Rep78 restricts adenovirus E1B55K-mediated p53 nuclear exportation显示文摘p53 的 Inactivation 在侵入人体气管粘膜的病菌类型 5 DNA 复制期间被需要。E1B55K,侵入人体气管粘膜的病菌早蛋白质,被报导了与 p53 交往并且禁止 p53 transactivation。以前的研究证明了联系 adeno 的病毒(AAV ) 类型 2 能从调停侵入人体气管粘膜的病菌的降级由 rescuing p53 减少侵入人体气管粘膜的病菌的转变潜力,但是细节还不是清楚的。我们由 co-immunoprecipitation 试金检测了 Rep78p53 相互作用。合作本地化试金表明 Rep78 禁止调停 E1B55K 的 p53 原子出口。然而, Rep78 detectably 没影响 p53 稳定性并且不能减轻 p53 的 transcriptional inactivation,当 E1B55K 不能被 Rep78 从 p53E1B55K 建筑群代替。我们的结果揭示 AAV-2 Rep78 禁止的新可能的机制由重新本地化的侵入人体气管粘膜的病菌 5 在原子核的 p53,可以在它的助手病毒上阐明 AAV-2 的规章的机制,侵入人体气管粘膜的病菌。 | Jingjing Wang Wenjuan Li Ran Wang Jinglun Xue Jinzhong Chen | 2013 | Acta Biochimica et Biophysica Sinica2013，45，2： | 2 |
| 5 | Axisymmetric-fold combination laser resonator 显示文摘 | Yude Li Jinglun Liu Mei Chen Junping Guo | 2005 | Optical Engineering v44 n62005，64，204： | 1 |
| 6 | Axisymmet- ric-fold combination laser resonator显示文摘 | Yude Li Jinglun Liu Mei Chen | 2005 | Optical Engi- neering2005，44，6： | 1 |
| 7 | Axisymmetric-fold combination laser resonator 显示文摘 | Yude Li Jinglun Liu Mei Chen | 2005 | Optical Engineering2005，44，06： | 1 |
| 8 | Axisymmetrie-fold combination laser resonator显示文摘 | Jinglun Liu | 1996 | OPtical Engineering1996，16，2： | 1 |
| 9 | Axisymmetric-fold combination laser resonator显示文摘 | Jinglun Liu Mei Chen | 2005 | Optical Engineering2005，44，6： | 1 |
| 10 | Axisymmetric-foidcombination laser resonator 显示文摘 | Yude Li Jinglun Liu Mei Chen | 2005 | Optical Engineering2005，44，6： | 1 |
| 11 | Research on near field distribution of axisymmetric folded-combined CO2 laser显示文摘 | Yude Li Junping Guo | 2006 | Optical Engineering2006，45，7： | 1 |
| 12 | Phase-locking re-sonator with annular gain media 显示文摘 | Chen Mei Li Yude Liu Jinglun | 2008 | Optics Communica-tions2008，281，13： | 1 |
| 13 | Proposal to obtain a hollow laser beam from a hollow cone-shaped resonator 显示文摘 | Jinglun Liu Yude Li | 2007 | Optical Engineering2007，46，10： | 1 |
| 14 | Human clotting factor Ⅸ (hF Ⅸ ) secretion in goat milk after direct transfer with hF Ⅸ minigene into mammary gland by using retroviral vectors显示文摘THE mammary gland bioreactor system of lactating animal, established by germ-linemicroinjection and embryo transplantation, has been one of the most exciting projects in thefield of biotechnology. However, this system is costly, laborious and tim-cosuming. In 1994,Johanna et al. reported that secretion of a foreign protein, human growth hormone (hGH),in milk had been achieved after direct introduction of the cDNA into the mammary gland ofgoats by replication-defective retroviral vector. In that work, the in vivo expression of | ZHANG Kezhong, WANG Hongwei, BAO Yun, LU Daru,XUE Jinglun (J. L. Hsueh), QIU Xinfang, HUANG Shuzhen,HUANG Ying, LI Bingyan, LI Hua and ZENG Yitao1. Institute of Genetics, Fudan University, Shanghai 200433, China 2 Shanghai Children’s Hospital, Shanghai Institute of Medical Genetics, Shanghai 200040, China | 1997 | Chinese Science Bulletin1997，42，15： | 1 |
| 15 | Synthesis and Improved Properties of Hypergolic Boronium-Based Ionic Liquids显示文摘一系列不对称的 monoimidazolium 基于 dihydroboronium 的离子的液体(IL ) 从 amine-boranes 被综合。所有产生 IL 被 1 H 和 13 C NMR,红外光谱学,元素的分析或高分辨率集中光谱。与对称的 bisimidazolium 相比,基于 dihydroboronium 的 IL,有更短的点火延期的展出改进性质预定的这些新 IL (标志) ,更高的密度,和更低的阶段转移作为绿燃料显示出有希望的申请潜力的温度。 | Xingye Li Huijie Lu Qi Wang Jinglun Huang Fude Nie Haibo Li Fu-Xue Chen | 2016 | Chinese Journal of Chemistry2016，34，7： | 1 |
| 16 | Research on near-field distributions of axisymmetric folded-combined CO2 laser 显示文摘 | Jinglun Liu Yude Li | 2006 | Optical Engineering2006，0742，： | 1 |
| 17 | Axisymmetrie-fold combination laser resonator显示文摘 | Li Yude Liu Jinglun Chen Mei | 2005 | Opt Eng2005，44，06： | 1 |
| 18 | Research on near-field distributions of axisymmetric folded-combined CO2 laser显示文摘 | Liu Jinglun Li Yude Guo Junping | 2006 | Opt Eng2006，45，07： | 1 |
| 19 | Combinatorial analysis of transcription and metabolism reveals the regulatory network associated with antioxidant substances in waxy corn显示文摘Maize is an essential source of nutrition for humans and animals and is rich in various metabolites that determine its quality.Different maize varieties show significant differences in metabolite content.Two kinds of waxy maize parental materials,S181 and 49B,created by the Chongqing Academy of Agricultural Sciences,are widely grown in China.S181 shows higher starch and sugar contents than 49B.This study generated metabolic profiles to assess the differences between the two varieties.A total of 674 metabolites that were significantly differentially expressed between the two varieties were identified by gas chromatography and untargeted metabolomics technology.These metabolites were associated with 21 categories,including antioxidant metabolites.Moreover,6415 differentially expressed genes(DEGs)were identified by RNA-seq.Interestingly,these DEGs comprised starch and sugar synthesis pathway genes and 72 different transcription factor families.Among these,six families that were reported to play an essential role in plant antioxidant action accounted for 39.2%of the transcription factor families.Using the Kyoto Encyclopedia of Genes and Genomes(KEGG)classification,the DEGs were mainly involved in amino acid biosynthesis,glycolysis/glucose metabolism,and the synthetic and metabolic pathways of antioxidant active substances.Furthermore,the correlation analysis of transcriptome and metabolomics identified five key transcription factors(ZmbHLH172,ZmNAC44,ZmNAC-like18,ZmS1FA2,ZmERF172),one ubiquitin ligase gene(ZmE25A)and one sucrose synthase gene(ZmSS1).They likely contribute to the quality traits of waxy corn through involvement in the metabolic regulatory network of antioxidant substances.Thus,our results provide new insights into maize quality-related antioxidant metabolite networks and have potential applications for waxy corn breeding. | Jianhong Ke Rui Wang Bangqian Song Jinglun Du Xiaojiao Li Ningning Song Zhirong Cai Rongli Chen Honghua Yi Xiangyin Lu Chen Jiang Zhengguo Li Baowen Huang | 2022 | Food Quality and Safety2022，6，4： | 0 |
| 20 | Multifunctional metal-polyphenol nanocomposite for melanoma targeted photo/chemodynamic synergistic therapy显示文摘Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species(ROS)by consuming H_(2)O_(2) in TME,improving the inadequate ROS generation problem of PDT.Therefore,synergistic therapy combining PDT and Fenton response-based CDT is a promising ap-proach for cancer treatment.Herein,a metal-polyphenol nanocomposite was deposited with gallic acid grafted hyaluronic acid and Fe^(3+) to contrast a Ce6 nano-delivery system(Ce6@HSF NPs)for melanoma synergistic therapy.Ce6@HSF NPs could be used as a Fenton reagent to induce the·OH production and enhance the PDT effect of Ce6 to a certain extent.After 4 h of cellular uptake,the fluorescence intensity of Ce6 in the Ce6@HSF NPs group was higher than 3 times that in the Ce6 group.The intracellular ROS generation level of the Ce6@HSF NPs(L)group combining CDT and PDT was higher than that of the Ce6 group and Ce6@HSF NPs group.In vitro and in vivo anti-melanoma studies show that Ce6@HSF NPs(L)group exhibited better anti-melanoma than other groups.Together,Ce6@HSF NPs provide a promising synergistic treatment potential for melanoma. | Di Chuan Huan Hou Yuelong Wang Min Mu Jinglun Li Yangmei Ren Na Zhao Bo Han Haifeng Chen Gang Guo | 2023 | Journal of Materials Science & Technology2023，，21： | 0 |
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