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| 1 | Interferon-α response in chronic hepatitis B-transfected HepG2.2.15 cells is partially restored by lamivudine treatment显示文摘AIM: To characterize the IFN-response and its modul- ation by the antiviral compound lamivudine in HBV- transfected HepG2.2.15 cells. METHODS: HepG2.2.15 and HepG2 cells were stimulated with various concentrations of IFN-a2a in the presence or absence of lamivudine. Then, total RNA was extracted and analysed by customised cDNA arrays and northern blot for interferon-inducible genes (ISGs). In addition, cellular proteins were extracted for EMSA and western blot. HBV replication was assessed by southern blot or ELISAs for HBsAg and HBeAg. RESULTS: Two genes (MxA, Cig5) with completely abolished and 4 genes (IFITM1, -2, -3, and 6-16) with partially reduced IFN-responses were identified in HepG2.2.15 cells. In 2 genes (IFITM1, 6-16), the response to IFN-a could be restored by treatment with lamivudine. This effect could not be explained by a direct modulation of the Jak/Stat signalling pathway since EMSA and western blot experiments revealed no suppression of Stat1 activation and ISGF3 formation after stimulation with IFN-a in HepG2.2.15 compared to HepG2 cells. CONCLUSION: These results are consistent with the assumption that chronic hepatitis B may specifically modulate the cellular response to IFN by a selective blockage of some ISGs. Antiviral treatment with lamivudine may partially restore ISG expression by reducing HBV gene expression and replication. | Shi-He Guan Mengji Lu Petra Grünewald Michael Roggendorf Guido Gerken Joerg F Schlaak | 2007 | World Journal of Gastroenterology2007，13，2： | 22 |
| 2 | Transarterial chemoembolization using degradable starch microspheres and iodized oil in the treatment of advanced hepatocellular carcinoma: evaluation of tumor response, toxicity, and survival显示文摘BACKGROUND: In a multidisciplinary conference patients with advanced non-resectable hepatocellular carcinoma (HCC) were stratified according to their clinical status and tumor extent to different regional modalities or to best supportive care. The present study evaluated all patients who were stratified to repeated transarterial chemoembolization (TACE) from 1999 until 2003 in terms of tumor response, toxicity, and survival. A moderate embolizing approach was chosen using a combination of degradable starch microspheres (DSM) and iodized oil (Lipiodol) in order to combine anti-tumoral efficiency and low toxicity. METHODS: Fourty-seven patients were followed up prospectively. TACE treatment consisted of cisplatin (50 mg/m2), doxorubicin (50 mg/m2), 450-900 mg DSM, and 5-30 ml Lipiodol. DSM and Lipiodol were administered according to tumor vascularization. Patient characteristics,toxicity, and complications were outlined. In multivariate regression analyses of pre-treatment variables from a prospective database, predictors for tumor response and survival after TACE were determined. RESULTS: 112 TACE courses were performed (2.4±1.5 courses per patient). Mean maximum tumor size was 75 (± 43) mm, in 68% there was bilobar disease. Best response to TACE treatment was: progressive disease (PD) 9%, stable disease (SD) 55%, partial remission (PR) 36%, and complete remission (CR) 0%. Multivariate regression analyses identified tumor size ≤75 mm, tumor number ≤5, and tumor hypervascularization as predictors for PR. The overall 1-, 2-, and 3-year-survival rates were 75%, 59%, and 41%, respectively, and the median survival was 26 months. Low α-fetoprotein levels (<400 ng/ml) (Odds ratio=3.3) and PR as best response to TACE (Odds ratio=6.7) were significantly associated with long term survival (>30 months, R2=36%). Grade 3 toxicity occurred in 7.1% (n=8), and grade 4 toxicity in 3.6% (n=4) of all courses in terms of reversible leukopenia and thrombocytopenia. The incidence of major complications was 5.4% (n=6). All complications were managed conservatively. The mortality within 6 weeks after TACE was 2.1% (one patient). CONCLUSIONS: DSM and Lipiodol were combined successfully in the palliative TACE treatment of advanced HCC resulting in high rates of tumor response and survival at limited toxicity. Favourable tumor response was associated with tumor extent and vascularization. TACE using DSM and Lipiodol can be considered a suitable palliative measure in patients who might not tolerate long acting embolizing agents. | Timm D Kirchhoff Joerg S Bleck Arne Dettmer Ajay Chavan Herbert Rosenthal Sonja Merkesdal Bernd Frericks Lars Zender Nisar P Malek Tim F Greten Stefan Kubicka Michael P Manns Michael Galanski | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，3： | 15 |
| 3 | Preclinical development of TLR ligands as drugs for the treatment of chronic viral infections显示文摘 | Xiaoyong Zhang Anke Kraft Ruth Broering Joerg F Schlaak Ulf Dittmer Mengji Lu | 2012 | Expert Opinion on Drug Discovery2012，，7： | 3 |
| 4 | Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array 显示文摘 | Wang SC Debbie W Kerrie F Alexandra A Shiaoman C Bevan EH Marco M Silvio S Sara GM Luigi C Anna MM Alex W Stuart S Gary B Ralf W Joerg P International Wheat Genome Sequencing Consortium Morten L Diane M Rudi A Rudy D Gina BG Abraham K Alina RA Catherine F Jerome S Michele M Curtis P Luo MC Jan DMM Jorge D Martin G Roberto T Cindy L Ivan M Colin C Keith JE Matthew H Eduard A | 2014 | Plant Biotechnol J2014，12，： | 1 |
| 5 | Germ cell transplantation in an azoospermic klinefelter bull显示文摘 | Janett F Schlatt S | 2003 | Biol Reprod2003，69，6： | 1 |
| 6 | Towards the Multimodal Transport of People and Freight: Inter connective Networks in the RheinRuhr Metropolis 显示文摘 | JOERG S ALEXANDER S ANDRE F | 2003 | Journal of Transport Geography2003，，11： | 1 |
| 7 | Generation of mature murine monocytes from heterogeneous bone marrow and de scription of their properties显示文摘 | Alexander F Joerg H Soenke W | 2011 | J Histochemistry & Cytochemis- try2011，59，9： | 1 |
| 8 | Analgesic and antihyperalgesic properties of propofol in a human pain model显示文摘 | Oliver B Joerg F Harald I | 2010 | Anesthesiology2010，113，2： | 1 |
| 9 | Filter-based active damping of voltage source converters with LCL filter显示文摘 | JOERG D MARCO L FRIEDRICH W F | | 0，，08： | 1 |
| 10 | The in vitro effect of fluor- idated milk in a bacterial biofilm- enamel model 显示文摘 | Wolfgang HA Stefan F Joerg H | 2006 | Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub2006，150，1： | 1 |
| 11 | Germ cell transplantation in an azoospermic klinefelter bull显示文摘 | Joerg H Janett F Schlatt S | | 0，，06： | 1 |
| 12 | Limitations of voltage -oriented PI current control of grid-connected PWM rectifiers with LCL filters显示文摘 | JOERG D WESSELS C FUCHS F W | 2009 | IEEE Transactions on Industrial Electronics2009，56，2： | 1 |
| 13 | Regulation of RAF activity by14-3-3proteins显示文摘 | Andreas F Angela B Joerg R | 2009 | The Journal of Biological Chemistry2009，284，5： | 1 |
| 14 | Social market economy as Europe's Social Model 显示文摘 | Joerges C Rodl F | | A European social citizenship0，200，： | 1 |
| 15 | ULTIMATE: Metallic TPS for Future RLV's-flrst results of ground& flight test campaigns 显示文摘 | Wolfgang P F Joerg B | 2006 | AIAA2006-29502006，2950，： | 1 |
| 16 | Steepest descent methods for multi-criteria optimization 显示文摘 | JoErg F Benar F S | 2000 | Mathematical Methods of Operations Research2000，51，3： | 1 |
| 17 | Nat Cell Biol:研究发现人肝细胞抵抗HCV感染的代谢过程显示文摘病毒感染是二十一世纪面临的主要医学挑战之一,从影响全球3%人口的丙肝病毒(HCV)流行病到最近爆发的西尼罗河病毒、寨卡病毒和埃博拉病毒感染。病毒缺乏进行自我复制所需的基础代谢机制。为了解决这个问题,它们劫持它们的宿主的代谢机制以便完成它们的生命周期和进行增殖。然而,科学家们仍然并没有很好地理解病毒与它们感染的有机体之间在代谢上的相互作用。这主要是因为人基因与代谢过程之间存在复杂的相互作用。 | Gahl Levy, Naomi Habib, Maria Angela Guzzardi, Daniel Kitsberg, David Bomze, Elishai Ezra, Merav Cohen, Nir Friedman Yaakov Nahmias Gahl Levy, Daniel Kitsberg, David Bomze, Merav Cohen, Nir Friedman Yaakov Nahmias Naomi Habib Maria Angela Guzzardi Elishai Ezra Basak E Uygun Korkut Uygun Martin Trippler Joerg F Schlaak Oren Shibolet Ella H Sklan Joerg Timm | 2016 | 现代生物医学进展2016，16，35： | 0 |
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