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| 1 | Dopaminergic effects on in vitro osteogenesis显示文摘Multiple growth factors(e.g., BMP2, TGF-b1, FGF2) and isolated genes have been shown to improve osteoblastic proliferation and mineralization, advancing bone tissue engineering. Among these factors, both polydopamine(PDA) and dopamine(DA) monomer have recently been reported to increase osteoblast proliferation and mineralization in vitro. Although a well-characterized neurotransmitter, DA's role in the bone is unknown. We hypothesize that DA can directly act on osteoblasts, and examined whether osteoblasts express DA receptors that respond to exogenous DA. m RNAs and protein cell lysates were obtained from MC3T3-E1 cells during osteogenic differentiation phase. Reverse transcription polymerase chain reaction and western blot analysis were used to examine the expression of DA receptors, D1–D5. Dose-response effect and time course of DA treatment on cell proliferation, mineralization, and osteogenic differentiation were investigated at pre-determined days. Real-time PCR was performed to investigate whether DA affects osteogenic gene expression(ALP, BSP, OC, OSX, RUNX2, and Collagen1a2) with or without receptor antagonists(SCH233390 and GR103691). Two-way ANOVA was used for statistical analysis. All five DA receptors(D1, D2, D3, D4, and D5) m RNAs and proteins were expressed in MC3T3-E1 cells. DA treatment increased cell proliferation for up to 7 days(P, 0.05). Osteogenic mineralization was significantly greater in the DA-treated group than control group(P, 0.05). Finally, expression of all the osteogenic genes was inhibited by DA receptor antagonists for D1, D3, and D5. Our findings suggest that MC3T3-E1 osteoblasts express functional DA receptors that enhance proliferation and mineralization. PDA is not biologically inert and has important implications in orthopedic applications. Furthermore, osteoblast differentiation might be regulated by the nervous system, presumably during bone development, remodeling, or repair. | Dong Joon Lee Henry C Tseng Sing Wai Wong Zhengyan Wang Meng Deng Ching-Chang Ko | 2015 | Bone Research2015，3，3： | 3 |
| 2 | On the twist performance of a multiple-cell active helicopter blade显示文摘 | Cesnik C E S Shin Sang Joon | 2001 | Smart Materials and Structures2001，10，1： | 1 |
| 3 | Discriminative weight training for a statistical model-based voice activity detection显示文摘 | Sang I K Jo Q H Joon H C | 2008 | IEEE Signal Processing Letters2008，15，： | 1 |
| 4 | Identification of tumor suppressor loci on the long arm of chromosome 4 in primary small cell lung cancers显示文摘 | Eun SC Joon C Kyung YC | 2002 | Yonsei Medical Journal2002，2，43： | 1 |
| 5 | Electrosteric stabilization of concentrated cement suspensions imparted by a strong anionic polyelectrolyte and a non-ionic polymer显示文摘 | Stacy G Bike Victor C Li | 2006 | Cement and Concrete Research2006，36，： | 1 |
| 6 | The chemistry of water treatment processes involving ozone, hydrogen peroxide and ultraviolet radiation显示文摘 | Willian H C Joon Wun K Douglas H C | 1987 | Ozone Sci Eng1987，9，： | 1 |
| 7 | The Sprengel deformity 显示文摘 | Tae - Joon C | 2000 | J Bone Joint Surg2000，82，： | 1 |
| 8 | Nonlinear instrumental variable estimation of an autoregression显示文摘 | Peter C B Phillips Joon Y Park Yoosoon Chang | 2004 | Journal of Econometrics2004，118，： | 1 |
| 9 | Development of a newbiomonitoring method to detect the abnormal activity ofDaphnia magna using automated Grid Counter device显示文摘 | Junho J Joon H K Byung C L | 2008 | Science of the total environment2008，389，： | 1 |
| 10 | An in-vitro evaluation of the cellular uptake and intraphagocytic bioactivity of clarithromycin, a new macrolide antimicrobial agent 显示文摘 | ANDERSON R JOONE G RENSBURG C E | 1988 | J Antimicrob Chemother1988，22，6： | 1 |
| 11 | The chemistry of water treatment process involving ozone, hydrogen peroxide, and ultraviolet radiation 显示文摘 | WILLIAM H G JOON W K DOUGLAS H C | 1987 | Ozone Science and Engneering1987，9，4： | 1 |
| 12 | Fast pyrolysis of Miscanthus sinensis in fluidized bed reactors: characteristics of product yields and biocrude oil quality显示文摘 | Jill P B Hang S C Joon W C Yeon S C | 2013 | Energy2013，60，： | 1 |
| 13 | Phase relation and dielectric properties in the Bi2P3-ZnO-Ta2O5 System显示文摘 | JOON H SOGABE T RANDALL C | 2001 | J Am Ceram Soc2001，84，11： | 1 |
| 14 | Controlled release of insulin from injectable Biodegradable triblock copolymer显示文摘 | Young J K Suna C Joon K | 2001 | Pharm Res2001，18，4： | 1 |
| 15 | Alpha-tocopherol antagonizes the multidrug resistance reversal activity of cyclosporin A,Verapamil,GF120918,Clofazimine and B669显示文摘 | Van Rensburg C E Joone G Anderson R | 1998 | Cancer letters1998，127，12： | 1 |
| 16 | Recent develop- ments of metal oxide semiconductors as photocatalysts in ad- vanced oxidation processes (AOPs) t'or treatment of dye waste-water显示文摘 | Samuel H S C Ta Y W Joon C J | 2011 | J Chem Techn Biotechn2011，86，9： | 1 |
| 17 | Genetic diversity within the Albugo candida complex (Peronosporales,Oomycota) inferred from phylogenetic analysis of ITS rDNA and COX2 mtDNA sequences显示文摘 | Young Joon C Seung-Beom H Hyeon Dong S | 2006 | Mol Phylogenet Evol2006，40，2： | 1 |
| 18 | Different clinical outcomes of acute kidney injury according to acute kidney injury network criteria in patients between ST elevation and non-ST elevation myocardial infarction显示文摘 | Seung Hwan Hwang Myung Ho Jeong Khurshid Ahmed Min Chul Kim Kyung Hoon Cho Min Goo Lee Jum Suk Ko Keun Ho Park Doo Sun Sim Nam Sik Yoon Hyun Ju Yoon Kye Hun Kim Young Joon Hong Hyung Wook Park Ju Han Kim Young keun Ahn Jeong Gwan Cho Jong Chun Park Jung C | 2011 | International Journal of Cardiology2011，，1： | 1 |
| 19 | An MCMC sampling approach to estimation of nonstationary hidden Markov models显示文摘 | DJURIC P M JOON H C | 2002 | IEEE Transactions on Signal Processing2002，50，5： | 1 |
| 20 | Use o显示文摘 | Sche//er E G Venter G J Joone C | 2011 | Onderstepoort J Vet Res2011，78，1： | 1 |
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