|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility显示文摘Objective: To examine the effect of pSer9-GSK-3β on breast cancer and to determine whether the underlying metabolic and immunological mechanism is associated with ROS/eIF2B and natural killer(NK) cells.Methods: We employed TWS119 to inactivate GSK-3β by phosphorylating Ser9 and explored its effect on breast cancer and NK cells. The expression of GSK-3β, natural killer group 2 member D(NKG2D) ligands, eIF2B was quantified by PCR and Western blot. We measured intracellular reactive oxygen species(ROS) and mitochondrial ROS using DCFH-DA and MitoSOX^(TM) probe,respectively, and conducted quantitative analysis of cellular respiration on 4T1 cells with mitochondrial respiratory chain complex Ⅰ/Ⅲ kits.Results: Our investigation revealed that TWS119 downregulated NKG2D ligands(H60 a and Rae1), suppressed the cytotoxicity of NK cells, and promoted the migration of 4T1 murine breast cancer cells. Nevertheless, LY290042, which attenuates p-GSK-3β formation by inhibiting the PI3K/Akt pathway, reversed these effects. We also found that higher expression of p Ser9-GSK-3β induced higher levels of ROS, and observed that abnormality of mitochondrial respiratory chain complex Ⅰ/Ⅲ function induced the dysfunction of GSK-3β-induced electron transport chain, naturally disturbing the ROS level. In addition, the expression of NOX3 and NOX4 was significantly up-regulated, which affected the generation of ROS and associated with the metastasis of breast cancer. Furthermore, we found that the expression of pSer535-eIF2B promoted the expression of NKG2D ligands(Mult-1 and Rae1) following by expression of pSer9-GSK-3β and generation of ROS.Conclusions: The PI3K/Akt/GSK-3β/ROS/eIF2B pathway could regulate NK cell activity and sensitivity of tumor cells to NK cells,which resulted in breast cancer growth and lung metastasis. Thus, GSK-3β is a promising target of anti-tumor therapy. | Fengjiao Jin Zhaozhen Wu Xiao Hu Jiahui Zhang Zihe Gao Xiao Han Junfang Qin Chen Li Yue Wang | 2019 | Cancer Biology & Medicine2019，16，1： | 20 |
| 2 | Value of magnetic resonance imaging including dynamic contrast-enhanced magnetic resonance imaging in differentiation between inverted papilloma and malignant tumors in the nasal cavity显示文摘 | Wang Xinyan Zhang Zhengyu Chen Xiaoli Li Jing Xian Junfang | 2014 | Chinese Medical Journal2014，，9： | 13 |
| 3 | Research and Practice of Dynamic Network Security Architecture for IaaS Platforms显示文摘Network security requirements based on virtual network technologies in IaaS platforms and corresponding solutions were reviewed.A dynamic network security architecture was proposed,which was built on the technologies of software defined networking,Virtual Machine(VM)traffic redirection,network policy unified management,software defined isolation networks,vulnerability scanning,and software updates.The proposed architecture was able to obtain the capacity for detection and access control for VM traffic by redirecting it to configurable security appliances,and ensured the effectiveness of network policies in the total life cycle of the VM by configuring the policies to the right place at the appropriate time,according to the impacts of VM state transitions.The virtual isolation domains for tenants’VMs could be built flexibly based on VLAN policies or Netfilter/Iptables firewall appliances,and vulnerability scanning as a service and software update as a service were both provided as security supports.Through cooperation with IDS appliances and automatic alarm mechanisms,the proposed architecture could dynamically mitigate a wide range of network-based attacks.The experimental results demonstrate the effectiveness of the proposed architecture. | Lin Chen Xingshu Chen Junfang Jiang Xueyuan Yin Guolin Shao | 2014 | Tsinghua Science and Technology2014，19，5： | 6 |
| 4 | Audiological evaluation in Chinese patients with mitochondrial encephalomyopathies显示文摘 | Liu Yuhe Xue Junfang Zhao Danhua Chen Li Yuan Yun Wang Zhaoxia | 2014 | Chinese Medical Journal2014，，12： | 6 |
| 5 | Tribological Behavior of Nano-ZrO2 Reinforced PTFE-PPS Composites显示文摘Polytetrafluoroethylene (PTFE) is a commonly used seal material for oil-free engine that is well known for its excellent tribological properties. In this work, the nano-ZrO2 particles were used as the friction modifiers to improve the friction and wear performance of PTFE-PPS composites. The friction and wear characteristics of PTFE/PPS-nano-ZrO2 composites were investigated by a block-on-ring tester under dry friction sliding condition. The worn surfaces, counterpart transfer films and wear debris were studied by scanning electron microscopy and X-ray photoelectron spectroscopy. It was found that the increase of nano- ZrO2 content could effectively reduce the coefficient of friction and enhance the anti-wear ability of PTFEPPS composites. Especially, the best tribological properties of the composites were obtained when the particle content of nano-ZrO2 was 10 vol%, the anti-wear performance of composite is 195 times better than that of the unfilled PTFE-PPS composite. Under different conditions, the coefficient of friction of PTFE/PPS-nano-ZrO2 composites was more affected by the applied load while the wear rate was more affected by the sliding velocity. | 曹文翰 龚俊 QI Yuan YANG Dongya GAO Gui 王宏刚 REN Junfang CHEN Shengsheng | 2019 | Journal of Wuhan University of Technology(Materials Science)2019，34，3： | 5 |
| 6 | Effects of blade sliding cutting angle and stem level on cutting energy of rice stems显示文摘Previous studies highlighted the significance of optimizing the cutting blade for crop harvesting and size reduction.This study investigated the effect of blade sliding cutting angle and stem level on cutting energy of single rice stem using a cutting apparatus that combined with texture analyzer.The cutting energy was determined for four blade angles.The results showed that the average cutting energy was the highest for cutting stem upper level and the lowest for cutting stem lower level.It was found that the peak cutting force per unit stem area decreased with blade sliding cutting angle increased.However,the least average cutting energy was 9.12 J/mm^(2) of 45°sliding angle when cutting without counter support blade and 32.3%less than that of 60°sliding angle.When cutting with counter support blade,the cutting energy per unit stem area varied from 6.57 to 12.54 J/mm 2 as the sliding angle varied from 0°to 60°,whereas the peak cutting force per unit stem area varied from 2.46 to 0.98 N/mm 2.It was concluded that the optimal sliding cutting angle was 45°without support blade and 30°with support blade,respectively.The experiments on rice stems in this study indicated that optimization of sliding cutting angle and stem level have a significant effect on cutting energy savings.Also this study emphasized the need to further investigate the effect of the case of more moisture content and cutting speed on the cutting energy to help in selection of optimum cutting speed and harvesting time. | Chunling Zhang Liqing Chen Junfang Xia Jumin Zhang | 2019 | International Journal of Agricultural and Biological Engineering2019，12，6： | 2 |
| 7 | Electronic and elastic properties of LisN under different pressure显示文摘 | Li Wei Chen Junfang Wang Teng | 2010 | Physica B2010，405，1： | 1 |
| 8 | Characterization of spatial plasma distribution in a divergence-type ECR-PECVD system显示文摘 | Chen Junfang Fu Silie | 2006 | Vacuum2006，81，1： | 1 |
| 9 | Temporal-spatial distributions of road silt loadings and fugitive road dust emissions in Beijing from 2019 to 2020显示文摘Road silt loading(sL)is an important parameter in the fugitive road dust(FRD)emissions.In this study,the improved Testing Re-entrained Aerosol Kinetic Emissions from Roads(TRAKER)combined with the AP-42 method was firstly developed to quickly measure and estimate the sLs of paved roads in Beijing,China.The annual average sLs in Beijing was 0.59±0.31 g/m^(2)in 2020,and decreased by 22.4%compared with that in 2019.The seasonal variations of sLs followed the order of spring>winter>summer>autumn in the two years.The seasonal mean road sLs on the same type road in the four seasons presented a decline trend from^(2)019 to 2020,especially on the Expressway,decreasing 47.4%-72.7%.The road sLs on the different type roads in the same season followed the order of Major arterial∼Minor arterial∼Branch road>Express road,and Township road∼Country highway>Provincial highway∼National highway.The emission intensities of PM10 and PM_(2.5)from FRD in Beijing in 2020 were lower than those in 2019.The PM10 and PM_(2.5)emission intensities at the four planning areas in the two years all presented the order of the capital functional core area>the urban functional expansion area>the urban development new area>the ecological conservation and development area.The annual emissions of PM10 and PM_(2.5)from FRD in Beijing in 2020 were 74,886 ton and 18,118 ton,respectively,decreasing by∼33.3%compared with those in 2019. | Haibin Wang Lihui Han Tingting Li Song Qu Yuncheng Zhao Shoubin Fan Tong Chen Haoran Cui Junfang Liu | 2023 | Journal of Environmental Sciences2023，，10： | 1 |
| 10 | Characterizations of GaN film growth by ECR plasma chemical vapor deposition 显示文摘 | Silie Fu Junfang Chen Hongbin Zhang | 2009 | Journal of Crystal Growth2009，311，12： | 1 |
| 11 | A Study of Diamond- Like Carbon Thin Films Prepared by Microwave Plasma Chemical Vapour Deposition显示文摘 | Lai Xiuqiong Chen Junfang Fu Silie | 2007 | Plasma Science and Technology2007，9，44： | 1 |
| 12 | Molecular mapping of a nuclear male-sterility gene in sunflower (Helianthus annuus L.) using TRAP and SSR markers显示文摘 | Junfang Chen Jinguo Hu Brady A. Vick C. C. Jan | 2006 | Theoretical and Applied Genetics2006，，1： | 1 |
| 13 | Fractional Frequency Offset Estimation for OFDM Systems in Non-Cooperative Communication显示文摘The traditional fractional frequency offset(FFO) estimation schemes for orthogonal frequency division multiplexing(OFDM) in non-cooperative communication have the problems of susceptible performance with the frequency offset values and the number of OFDM symbols,a novel fractional frequency offset blind estimation scheme based on EKF for OFDM systems is conceived.The nonlinear function of the frequency offset is calculated by employing the correlation.And then the frequency offset is estimated by means of the iterative algorithm of EKF.The finally fractional frequency offset is estimated by adopting repeated the above process.Simulation results demonstrate that the proposed scheme is robust to the frequency offset values without any requirements of a prior knowledge. | Mingqian Liu Jian Chen Bingbing Li Junfang Li | 2016 | China Communications2016，13，9： | 1 |
| 14 | Identification of a two-domain antifreeze protein gene in Antarctic eelpout Lycodichthys dearborni显示文摘 | Junfang Zhang Cheng Deng Jianshe Wang Liangbiao Chen | 2009 | Polar Biology2009，，1： | 1 |
| 15 | Quality of life and related factors in the older rural and urban chinese populations in zhejiang province显示文摘 | Zhou Biao Kun Chen Wang Junfang | 2010 | J Applied Gerontol Online first published on February 162010，2010，： | 1 |
| 16 | Risk SNP-induced lncRNA-SLCCl drives colorectal cancer through activating glycolysis signaling显示文摘Long non-coding RNAs(lncRNAs)play key roles in colorectal carcinogenesis.Here,we aimed to identify the risk SNP-induced lncRNAs and to investigate their roles in colorectal carcinogenesis.First,we identified rs6695584 as the causative SNP in 1 q41 locus.The A>G mutation of rs6695584 created a protein-binding motif of BATF,altered the enhancer activity,and subsequently activated IncSLCCl expression.Further validation in two independent CRC cohorts confirmed the upregulation of IncSLCCl in CRC tissues,and revealed that increased IncSLCCl expression was associated with poor survival in CRC patients.Mechanistically,lncRNA-SLCCl interacted with AHR and transcriptionally activated HK2 expression,the crucial enzyme in glucose metabolism,thereby driving the glycolysis pathway and accelerating CRC tumor growth.The functional assays revealed that IncSLCCl induced glycolysis activation and tumor growth in CRC mediated by HK2.In addition,HK2 was upregulated in colorectal cancer tissues and positively correlated with IncSLCCl expression and patient survival.Taken together,our findings reveal a risk SNP-mediated oncogene lncRNA-SLCCl promotes CRC through activating the glycolysis pathway. | Tingting Yan Chaoqin Shen Penglei Jiang Chenyang Yu Fangfang Guo Xianglong Tian Xiaoqiang Zhu Shiyuan Lu Bingshe Han Ming Zhong Jinxian Chen Qiang Liu Yingxuan Chen Junfang Zhang Jie Hong Haoyan Chen Jing-Yuan Fang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 1 |
| 17 | Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish 显示文摘 | Zhang Junfang Zuozhou Chen Lei Chen | 2008 | Proceedings of the National Academy of Sciences of the United States of America2008，105，12： | 1 |
| 18 | Performance of aerobic rice varieties under irrigated conditions in North China显示文摘 | B.A.M. Bouman Xiaoguang Yang Huaqi Wang Zhimin Wang Junfang Zhao Bin Chen | 2005 | Field Crops Research2005，，1： | 1 |
| 19 | Alternative splicing of the cell fate determinant Numb in hepatocellular carcinoma显示文摘 | Yinying Lu Wanping Xu Junfang Ji Dechun Feng Carole Sourbier Youfeng Yang Jianhui Qu Zhen Zeng Chunping Wang Xiujuan Chang Yan Chen Alok Mishra Max Xu Min‐Jung Lee Sunmin Lee Jane Trepel W. Marston Linehan Xinwei Wang Yongping Yang Len Neckers | 2015 | Hepatology2015，，： | 1 |
| 20 | Characterization of spatial plasma distribution in a divergence-type ECRPECVD system显示文摘 | Chen Junfang Fu Silie Lai Xiuqiong | 2006 | Science Direct2006，81，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员