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| 1 | The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility显示文摘Objective: To examine the effect of pSer9-GSK-3β on breast cancer and to determine whether the underlying metabolic and immunological mechanism is associated with ROS/eIF2B and natural killer(NK) cells.Methods: We employed TWS119 to inactivate GSK-3β by phosphorylating Ser9 and explored its effect on breast cancer and NK cells. The expression of GSK-3β, natural killer group 2 member D(NKG2D) ligands, eIF2B was quantified by PCR and Western blot. We measured intracellular reactive oxygen species(ROS) and mitochondrial ROS using DCFH-DA and MitoSOX^(TM) probe,respectively, and conducted quantitative analysis of cellular respiration on 4T1 cells with mitochondrial respiratory chain complex Ⅰ/Ⅲ kits.Results: Our investigation revealed that TWS119 downregulated NKG2D ligands(H60 a and Rae1), suppressed the cytotoxicity of NK cells, and promoted the migration of 4T1 murine breast cancer cells. Nevertheless, LY290042, which attenuates p-GSK-3β formation by inhibiting the PI3K/Akt pathway, reversed these effects. We also found that higher expression of p Ser9-GSK-3β induced higher levels of ROS, and observed that abnormality of mitochondrial respiratory chain complex Ⅰ/Ⅲ function induced the dysfunction of GSK-3β-induced electron transport chain, naturally disturbing the ROS level. In addition, the expression of NOX3 and NOX4 was significantly up-regulated, which affected the generation of ROS and associated with the metastasis of breast cancer. Furthermore, we found that the expression of pSer535-eIF2B promoted the expression of NKG2D ligands(Mult-1 and Rae1) following by expression of pSer9-GSK-3β and generation of ROS.Conclusions: The PI3K/Akt/GSK-3β/ROS/eIF2B pathway could regulate NK cell activity and sensitivity of tumor cells to NK cells,which resulted in breast cancer growth and lung metastasis. Thus, GSK-3β is a promising target of anti-tumor therapy. | Fengjiao Jin Zhaozhen Wu Xiao Hu Jiahui Zhang Zihe Gao Xiao Han Junfang Qin Chen Li Yue Wang | 2019 | Cancer Biology & Medicine2019，16，1： | 20 |
| 2 | Integrated Metabolite and Gene Expression Profiles Identify Lipid Biomarkers Associated With Progression of Hepatocellular Carcinoma and Patient Outcomes显示文摘 | Anuradha Budhu Stephanie Roessler Xuelian Zhao Zhipeng Yu Marshonna Forgues Junfang Ji Edward Karoly Lun–Xiu Qin Qing–Hai Ye Hu–Liang Jia Jia Fan Hui–Chuan Sun Zhao–You Tang Xin Wei Wang | 2013 | Gastroenterology2013，，5： | 1 |
| 3 | Ultralong organic room-temperature phosphorescence of electrondonating and commercially available host and guest molecules through efficient Förster resonance energy transfer显示文摘Ultralong organic room-temperature phosphorescence(RTP)materials have attracted tremendous attention recently due to their diverse applications.Several ultralong organic RTP materials mimicking the host-guest architecture of inorganic systems have been exploited successfully.However,complicated synthesis and high expenditure are still inevitable in these studies.Herein,we develop a series of novel host-guest organic phosphorescence systems,in which all luminophores are electron-rich,commercially available and halogen-atom-free.The maximum phosphorescence efficiency and the longest lifetime could reach 23.6%and 362 ms,respectively.Experimental results and theoretical calculation indicate that the host molecules not only play a vital role in providing a rigid environment to suppress non-radiative decay of the guest,but also show a synergistic effect to the guest through Förster resonance energy transfer(FRET).The commercial availability,facile preparation and unique properties also make these new host-guest materials an excellent candidate for the anti-counterfeiting application.This work will inspire researchers to develop new RTP systems with different wavelengths from commercially available luminophores. | Yeling Ning Junfang Yang Han Si Haozhong Wu Xiaoyan Zheng Anjun Qin Ben Zhong Tang | 2021 | Science China Chemistry2021，64，5： | 1 |
| 4 | IP-10 and fractalkine induce cytotoxic phenotype of murine NK cells显示文摘We characterized the murine NK cell subsets of the tumor microenvironment(TME)with low expressions of CD16 and NKG2D and investigated the chemokines that deter CD16~low NKG2D^low subsets.Our results demonstrated the activation of primary and KY-1 NK cell by ligands and found that exogenous CXCL10/interferon-gamma-induced protein 10(IP-10)and fractalkine(FKN)can up-regulate the expression of CD16 and NKG2D.Moreover,both IP-10 and FKN are shown to facilitate migration,adhesion and cytotoxicity of NK cell subsets of the TME,due to the up-regulated CD16 and NKG2D.Overall,our data provide a new path by which to enhance murine NK cell cytotoxic potential and improve the quality of NK cells of the TME. | Fang Liu Junfang Qin Hongyao Zhang Ning Li Meihua Shan Lan Lan Yue Wang | 2016 | Science Bulletin2016，61，3： | 0 |
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