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| 1 | Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues显示文摘BACKGROUND Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are different and depend on their tissue of origin. Understanding the factors that underlie MSC biology should constitute important points for consideration for researchers interested in clinical MSC application. AIM To characterize the biological activity of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SMMSCs), and skin (SK-MSCs). METHODS MSCs were isolated from the tissues, cultured for 10 passages, and assessed for: phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, stemness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc;27 bioactive factors were screened using the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was performed. RESULTS All MSCs showed the basic MSC phenotype;however, their expression decreased during the follow-up period, as confirmed by fluorescence intensity. The examined MSCs express CD146 marker associated with proangiogenic properties;however their expression decreased in AT-MSCs and SM-MSCs, but was maintained in BM-MSCs. In contrast, in SK-MSCs CD146 expression increased in late passages. All MSCs, except BM-MSCs, expressed PW1, a marker associated with differentiation capacity and apoptosis. BM-MSCs and AT-MSCs expressed stemness markers Sox2 and Oct4 in long-term culture. All MSCs showed a stable p53 and c-Myc expression. BM-MSCs and AT-MSCs maintained their differentiation capacity during the follow-up period. In contrast, SK-MSCs and SM-MSCs had a limited ability to differentiate into adipocytes. BM-MSCs and AT-MSCs revealed similarities in phenotype maintenance, capacity for multilineage differentiation, and secretion of bioactive factors. Because AT-MSCs fused with SM-MSCs as effectively as BM-MSCs, AT-MSCs may constitute an alternative source for BM-MSCs. CONCLUSION Long-term culture affects the biological activity of MSCs obtained from various tissues. The source of MSCs and number of passages are important considerations in regenerative medicine. | Urszula Kozlowska Agnieszka Krawczenko Katarzyna Futoma Tomasz Jurek Marta Rorat Dariusz Patrzalek Aleksandra Klimczak | 2019 | World Journal of Stem Cells2019，11，6： | 10 |
| 2 | Forming of magnesium alloy microtubes in the fabrication of biodegradable stents显示文摘Magnesium alloys have, in recent years, been recognized as highly promising biodegradable materials, especially for vascular stent applications. Forming of magnesium alloys into high-precision thin-wall tubes has however presented a technological barrier in the fabrication of vascular stents, because of the poor workability of magnesium at room temperature. In the present study, the forming processes, i.e., hot indirect extrusion and multi-pass cold drawing were used to fabricate seamless microtubes of a magnesium alloy. The magnesium alloy ZM21 was selected as a representative biomaterial for biodegradable stent applications. Microtubes with an outside diameter of 2.9 mm and a wall thickness of 0.2 mm were successfully produced at the fourth pass of cold drawing without inter-pass annealing. Dimensional evaluation showed that multipass cold drawing was effective in correcting dimensional non-uniformity arising from hot indirect extrusion. Examinations of the microstructures of microtubes revealed the generation of a large number of twins as a result of accumulated work hardening at the third and fourth passes of cold drawing, corresponding to the significantly raised forming forces. The work demonstrated the viability of the forming process route selected for the fabrication of biodegradable magnesium alloy microtubes. | Lixiao Wang Gang Fang Lingyun Qian Sander Leeflang Jurek Duszczyk Jie Zhou | 2014 | Progress in Natural Science:Materials International2014，24，5： | 2 |
| 3 | Heart rate turbulence-a new predictor of sudden cardiac death显示文摘 | Jurek S Sredniana B Musiulik-Lydka A Kalarus Z | 2007 | Pol Merkur Lekarski2007，23，133： | 1 |
| 4 | Postural imbalance and vibratory sensitivity in patients with idiopathic scoliosis:implications for treatment显示文摘 | Byl NN Holland S Jurek A | 1997 | J Orthop Sports Phys Ther1997，26，2： | 1 |
| 5 | Umbilical artery lactate level as marker of neonatal clinical condition-- preliminary results 显示文摘 | Jurek W Szymczakiewicz-Mutanowska AM Jurek G | 2001 | Przegl Lek2001，58，12： | 1 |
| 6 | Multipass cold drawing of magnesium alloy minitubes for biodegradable vascular stents显示文摘 | Gang Fang Wei-jiang Ai Sander Leeflang Jurek Duszczyk Jie Zhou | 2013 | Materials Science & Engineering C2013，，6： | 1 |
| 7 | Postural imbalance and vibratory sensitivity in patients with idiopathic scoliosis:implications for treatment显示文摘 | Byl NN Holland S Jurek A | 1997 | J Orthop Sports Phys Ther1997，26，2： | 1 |
| 8 | Liver steatosis in Polish children assessed by medicolegal autopsies 显示文摘 | Rorat M Jurek T Kuchar E | 2013 | World J Pediatr2013，9，1： | 1 |
| 9 | Selected ehiral al- cohols: Enzymic resolution and reduction of convenient sub- strates显示文摘 | JUREK O WIMMEROVA M WIMMER Z | 2008 | Coordin Chem Rev2008，252，5： | 1 |
| 10 | Heart rate turbulence - a new predictor of sudden cardiac death显示文摘 | Jurek S Sredniawa B Musialik- Lydka A | 2007 | Pol Merkur Lekarski2007，23，133： | 1 |
| 11 | The role of plasma membrane in bioreduction of two tetrazolium salts, MTT,and CTC显示文摘 | Tytus Bernas Jurek W Dobrucki | 2000 | Archives of Biochemistry and Biophysics2000，380，1： | 1 |
| 12 | Characterization of the cure reactions of bismaleimide composite matrices显示文摘 | Roger J. Morgan E. Eugene Shin Boris Rosenberg Ann Jurek | 1997 | Polymer1997，，3： | 1 |
| 13 | Biodegradation of pentachlorophenol by the white rot fungus Phanerochaete chrysosporium显示文摘 | Miteski G J Bumpns J A Jurek M A | 1988 | Applied and Environmental Microbiology1988，54，12： | 1 |
| 14 | Umbilical ar- tery lactate level as marker of neonatal clinical condition-prelimina- ry results 显示文摘 | Jurek W Szymczakiewicz-Mutanowska AM Jurek G | 2001 | Przegl Lek2001，58，12： | 1 |
| 15 | Energy efficiency in buildings through information – Swedish perspective显示文摘 | Jessica Henryson Teresa H?kansson Jurek Pyrko | 2000 | Energy Policy2000，，3： | 1 |
| 16 | Extrusion of 7075 aluminium alloy through double-pocket dies to manufacture a complex profile显示文摘 | Gang Fang Jie Zhou Jurek Duszczyk | 2008 | Journal of Materials Processing Tech2008，，6： | 1 |
| 17 | Effect of pocket design on rnetal flow through single-bearing extrusion dies to produce a thin-walled aluminium profile 显示文摘 | Gang Fang Jie Zhou Jurek Duszczyk | 2008 | Journal of Materials Processing Technology2008，199，： | 1 |
| 18 | Energy efficiency in buildings through information-Swedish perspective 显示文摘 | Jessica Henryson Teresa H?kansson Jurek Pyrko | 2000 | Energy Policy2000，28，3： | 1 |
| 19 | LDL susceptibility to oxidation and HDL antioxidant capacity in patients with renal failure显示文摘 | Jurek A Turyna B Kubit P | 2006 | Clin Biochem2006，39，： | 1 |
| 20 | Biodegradation and secondary effluent toxicity of ethoxylated suffactants显示文摘 | Jurek P Gregory W P | 1990 | Wat Res1990，24，8： | 1 |
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