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| 1 | 睾丸主要血管或睾丸网旁取精点与取精成功率不具显著相关性:以220例非梗阻性无精子症患者行传统或显微睾丸取精术为例显示文摘采用传统与显微睾丸取精术相结合方式对220例梗阻性无精症患者行精子提取术,如果传统手术方式未见精子即为阴性取精位点,然后选取2—3个阴性取精位点行显微睾丸取精术。术中通过手术显微镜评价睾丸血管系统,同时记录阳性取精位点与睾丸血供的关系。总的精子获取率为58-2%,早期应用传统手术方式精子获取率为46.8%,而后行显微睾丸取精术则精子获取率增加11.7%。故显微睾丸取精术的应用可显著增加精子获取率(P=0.017),且在睾丸网或睾丸主要血管处并不能显著增加阳性取精位点。 | J Ullrich Schwarzer Heiko Steinfatt Heiko Steinfatt Manfred Schleyer Frank M Kōhn Klaus Fiedler2, Irene von Hertwig Gottfried Krüsmann Wolfgang Würfel | 2013 | Asian Journal of Andrology2013，15，6： | 5 |
| 2 | PI 3-kinase pathway is responsible for antiapoptotic effects of atrial natriuretic peptide in rat liver transplantation显示文摘AIM:To investigate the in vivo effect of atrial natriureticpeptide(ANP)and its signaling pathway during ortho-topic rat liver transplantation.METHODS:Rats were infused with NaCl,ANP(5 μg/kg),wortmannin(WM,16 μg/kg),or a combination ofboth for 20 min.Livers were stored in UW solution(4°C)for 24 h,transplanted and reperfused.Apoptosis wasexamined by caspase-3 activity and TUNEL staining.Phosphorylation of Akt and Bad was visualized by West-ern blotting and phospho-Akt-localization by confocalmicroscopy.RESULTS:ANP-pretreatment decreased caspase-3activity and TUNEL-positive cells after cold ischemia,indicating antiapoptotic effects of ANP in vivo.The an-tiapoptotic signaling of ANP was most likely caused byphosphorylation of Akt and Bad,since pretreatment withPI 3-kinase inhibitor WM abrogated the ANP-inducedreduction of caspase-3 activity.Interestingly,analysis ofliver tissue by confocal microscopy showed translocationof phosphorylated Akt to the plasma membrane of hepa-tocytes evoked by ANP.CONCLUSION:ANP activates the PI-3-kinase pathwayin the liver in vivo leading to phosphorylation of Bad, an event triggering antiapoptotic signaling cascade inischemic liver. | Uwe Grutzner Melanie Keller Michael Bach Alexandra K Kiemer Herbert Meissner Manfred Bilzer Stefan Zahler Alexander L Gerbes Angelika M Vollmar | 2006 | World Journal of Gastroenterology2006，12，7： | 3 |
| 3 | Phylogenetic relationships among genera of Aphidiinae(Hymenoptera: Braconidae) based on DNA sequence of the mitochondrial 16S rRNA gene显示文摘 | SRINIVAS K WOLFGANG V MANFRED M | 2000 | Syst Ent2000，25，： | 2 |
| 4 | Structuralinvestigation of carbon/carbon composites by neutron scattering显示文摘 | MANFRED P GERHARD K HERWIG P | 2006 | Physica2006，，： | 1 |
| 5 | The vitamin E-binding protein afamin increases in maternal serum during pregnancy显示文摘 | Michael Hubalek Hannes Buchner Manfred G. M?rtl Dietmar Schlembach Berthold Huppertz Branka Firulovic Wolfgang K?hler Erich Hafner Benjamin Dieplinger Ludwig Wildt Hans Dieplinger | 2014 | Clinica Chimica Acta2014，，： | 1 |
| 6 | Dialysate sodium concentration and the association with interdialytic weight gain, hospitalization, and mortality显示文摘 | MANFRED H ANGELO K RAJIV S | 2012 | Clin J Am Soc Nephrol2012，7，： | 1 |
| 7 | Image fusion using thee hlers spectral characteristics preservation algorithm显示文摘 | Sascha K Manfred E | | 0，，02： | 1 |
| 8 | Environmental impact assessment including indirect effects-a case study using input-output analysis显示文摘 | MANFRED L SHAUNA A M BRITTA K | 2003 | Environmental Impact Assessment Review2003，23，3： | 1 |
| 9 | Greenbelt and Green Heart: Separating and Integrating Landscapes in European City Regions 显示文摘 | MANFRED K | 2002 | Landscape and Urban Planning2002，72，12： | 1 |
| 10 | Rapid Detection of Herpes- Simplex Virus and Varieella-Zoster Virus Infections by Real-Time PCR 显示文摘 | Manfred W Ursula M K Frank T | 2003 | J Clin Mierobiol2003，41，4： | 1 |
| 11 | DNA-based prediction of humanexternally visible characteristics in forensics:Motivations,scientific challenges,and ethical considerations显示文摘 | Manfred K Peter M S | 2009 | Forensic Sci Int:Genetics2009，3，： | 1 |
| 12 | Passive high-power microwave components 显示文摘 | Manfred K | 2002 | IEEE Transactions on Plasma Science2002，30，3： | 1 |
| 13 | An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow显示文摘 | Manfred BL Nicole K Alexandra L | 1999 | J Immunol Methods1999，223，1： | 1 |
| 14 | An efficient multiplexgenotyping approach for detecting the major worldwide humanY-chromosome haplogroup显示文摘 | Mannis V O Arwin R Manfred K | 2011 | Int J Legal Med2011，125，： | 1 |
| 15 | ^+2 ^2- : A new high-nitrogen high-energetic material显示文摘 | Gerhard H Manfred K Uldis T | 2001 | Inorg them2001，40，： | 1 |
| 16 | Mitochondrial sulfide oxidation in Arenicola marina, Evidence for alternative electron pathways显示文摘 | SUSANNE V MANFRED K G | 2004 | European Journal of Biochemistry2004，235，12： | 1 |
| 17 | Improved performance and cost reduction of RO seawater systems using UF pretreatment显示文摘 | Mark Wilf Manfred K Schierach | 2001 | Desalination2001，135，13： | 1 |
| 18 | Nanosatellite and microsystem technology capabilitis, limitation and applications显示文摘 | M Seven K Manfred L Norbert | 2005 | Acta Astron2005，39，4： | 1 |
| 19 | Greenbelt and Green Heart: separating and integrating landscapes in European city regions显示文摘 | Manfred Kühn | 2002 | Landscape and Urban Planning2002，，1： | 1 |
| 20 | Environmental impact assessment including indirect effects: A case study using input-output analysis 显示文摘 | MANFRED L SHAUNA A M BRITTA K | 2003 | Environmental Impact Assessment Re- view2003，23，： | 1 |
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