|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | Hepatic expression and cellular distribution of the glucose transporter family显示文摘Glucose and other carbohydrates are transported into cells using members of a family of integral membrane glucose transporter (GLUT) molecules. To date 14 members of this family, also called the solute carrier 2A proteins have been identified which are divided on the basis of transport characteristics and sequence similarities into several families (Classes 1 to 3). The expression of these different receptor subtypes varies between different species, tissues and cellular subtypes and each has differential sensitivities to stimuli such as insulin. The liver is a contributor to metabolic carbohydrate homeostasis and is a major site for synthesis, storage and redistribution of carbohydrates. Situations in which the balance of glucose homeostasis is upset such as diabetes or the metabolic syndrome can lead metabolic disturbances that drive chronic organ damage and failure, confirming the importance of understanding the molecular regulation of hepatic glucose homeostasis. There is a considerable literature describing the expression and function of receptors that regulate glucose uptake and release by hepatocytes, the most import cells in glucose regulation and glycogen storage. However there is less appreciation of the roles of GLUTs expressed by non parenchymal cell types within the liver, all of which require carbohydrate to function. A better understanding of the detailed cellular distribution of GLUTs in human liver tissue may shed light on mechanisms underlying disease pathogenesis. This review summarises the available literature on hepatocellular expression of GLUTs in health and disease and highlights areas where further investigation is required. | Sumera Karim David H Adams Patricia F Lalor | 2012 | World Journal of Gastroenterology2012，18，46： | 5 |
| 3 | p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival显示文摘响应 DNA 损坏的房间命运的中央仲裁人是 p53,它调整涉及房间周期拘捕,幸存和 apoptosis 的基因的表示。尽管 DNA 损坏开始的许多回答被描绘了,肌动朊细胞骨架管理者的角色大部分是未知的。我们现在显示出那 RhoC 和秘鲁利玛机场之代号 kinase (LIMK2 ) 2 是 genotoxic 代理人导致的直接 p53 目标基因。尽管 RhoC 和 LIMK2 在肌动朊细胞骨架规定有生长得很好的角色,我们的结果显示 LIMK2 的激活也有支持幸存的功能追随者 DNA 损坏。由调停 siRNA 的击倒或选择的药理学封锁的 LIMK 抑制敏化房间到无线电 -- 或化疗,以便当与 LIMK 抑制结合了时,当单身地管理了时,是尚不致命的治疗导致了房间死亡。我们的调查结果建议把 LIMK 禁止者与 genotoxic 治疗相结合能比单个代理人的管理更有效,并且加亮在肌动朊细胞骨架管理者和 DNA 之间的一个新奇连接导致损坏的房间幸存机制。 | Daniel R Croft Diane Crighton Michael S Samuel Filipe C Lourenco June Munro Jenifer Wood Karim Bensaad Karen H Vousden Owen J Sansom Kevin M Ryan Michael F Olson | 2011 | Cell Research2011，21，4： | 3 |
| 4 | Effect of isolation on fragility curves of highway bridges based on simplified approach 显示文摘 | KARIM K R YAMAZAKI F | 2007 | Soil Dynamics and Earthquake Engineering2007，27，5： | 1 |
| 5 | An adaptive middleware for contextsensitive communications for real-time applications in ubiquitous computing environments显示文摘 | Yau S S Karim F | 2004 | Real-Time Systems2004，26，1： | 1 |
| 6 | Assessing the impacts of climate change and dams on floodplaininundation and wetland connectivity in the wet-dry tropics of northern Australia 显示文摘 | KARIM F DUTTA D MARVANEK S | 2015 | Journal of Hydrology2015，522，： | 1 |
| 7 | Modification of the Phase Stability of Polymer Blends by Fillers显示文摘 | Karim A Liu D W Douglas J F | 2000 | Poly mer2000，41，： | 1 |
| 8 | Cell - cycle - dependent expression of transforming growth factor β type Ⅰ receptor correlates with differential proliferative effects if TGF - β1 in articular chondrocytes显示文摘 | Nathalie F Jean - Pierre P | 1998 | Experimental cell research1998，243，： | 1 |
| 9 | A blind source separation technique using second-order Statistics 显示文摘 | BELOOUCHRANI A KARIM A M CARDOSO J F | 1997 | IEEE Trans Signal Process1997，45，2： | 1 |
| 10 | Tumor-selective gene trans- duction and cell killing with an oncotropic autonomous parvovi- Pas-based vector显示文摘 | Dupont F Avalosse B Karim A | 2000 | Gene Ther2000，7，9： | 1 |
| 11 | Preparation and properties ofimmobilized pummelo limonoid glucosyltransferase显示文摘 | KARIM M HASHINAGA F | 2002 | ProcessBiochemistry2002，38，5： | 1 |
| 12 | Spinodal dewetting of thin polymer films 显示文摘 | Xie R Karim A Douglas J F Han C C Weiss R A | 1998 | Physical Review Letters1998，81，： | 1 |
| 13 | Recontlgurable Context - sensitive Middleware for Pervasive Computing 显示文摘 | Yau S S Karim F Wang Yu | 2002 | IEEE Pervasive Computing2002，13，： | 1 |
| 14 | Role of 5 hydroxytryptamine3 antagonists in the prevention o{ emesis caused by anticancer thera- py 显示文摘 | Karim F Roerig S C Saphier D | 1996 | Biochem Pharmacol1996，52，： | 1 |
| 15 | Fabrication of a non-ce- ment binder using slag, palm oil fuel ash and rice husk ash with so- dium hydroxide 显示文摘 | Karim M R Zain M F M Jamil M | 2013 | Construction and Building Materials2013，49，： | 1 |
| 16 | 显示文摘 | Karim F Isabelle C Jean PP | 1998 | Cancer1998，82，6： | 1 |
| 17 | Traffic shaping for MPEG video transmission over the next generation Internet显示文摘 | ALAM M F ATIQUZZAMAN M KARIM M A | 2000 | Computer Communications2000，23，1415： | 1 |
| 18 | A QTL affecting milk yield and composition maps to bovine chromosome 20: A confirmation 显示文摘 | Arranz J J Coppieters W Berzi P Cambisano N Grisart B Karim L Marcq F Moreau L Mezer C Riquet J Simon P Vanmanshoven P Wagenaar D and Georges M | 1998 | Animal Genetics1998，29，： | 1 |
| 19 | Role of 5-hydrox- ytrptamine(5-HT3) antagonists in the prevention of emeisi caused by anticancer therapy显示文摘 | Karim F Roerig SC Saphier D | 1996 | Biochem Pharmacol1996，52，5： | 1 |
| 20 | Regionalization of alarge-scale crop growth model for sub-Saharan Africa:Modelsetup,evaluation,and estimation of maize yields显示文摘 | Christian F Thomas G Karim C A | 2012 | Agriculture Ecosystems&Environment2012，151，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员