|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | New combination test for hepatitis C virus genotype and viral load determination using Amplicor GT HCV MONITOR test v2.0显示文摘AIM: To develop a new sensitive and inexpensive hepatitis C virus (HCV) combination test (HCV Guideline test) that enables the determination of HCV genotypes 1, 2 and 3,and simultaneous determination of HCV viral load using commercial Amplicor GT HCV MONITOR test v2.0 (microwell version).METHODS: The HCV Guideline test used the PCR product generated in commercial Amplicor GT HCV Monitor test v2.0 for viral load measurement using microwell plate version of Amplicor HCV Monitor and also captured on separate plates containing capture probes and competitive oligonucleotide probes specific for HCV genotypes 1, 2 and 3, The HCV genotype was subsequently determined using the biotin-labeled PCR product and five biotin-labeled HCV-specific probes.RESULTS: The sensitivity of the HCV Guideline test was 0.5 KIU/mL. Specificity of the HCV Guideline test was confirmed by direct sequencing of HCV core region and molecular evolutionary analyses based on a panel of 31 samples. The comparison of the HCV Guideline test and an in-house HCV core genotyping assay using 252 samples from chronic hepatitis C patients indicated concordant results for 97.2% of samples (59.5% genotype 1, 33.7% genotype 2, 6.0% genotype 3, and 0.8% mixed genotypes).Similarly, the HCV Guideline test showed concordance with a serological test, and the serological test failed to assign any serotype in 12.7% of the samples, indicating a better sensitivity of the HCV Guideline test.CONCLUSION: Clinically, both viral load and genotypes (1, 2 and 3) have been found to be major predictors of antiviral therapy outcome regarding chronic hepatitis C based on guidelines and they are, in normal circumstances,performed as separate stand-alone assays. The HCV Guideline test is a useful method for screening large cohorts in a routine clinical setting for determining the treatment regimen and for predicting the outcome of antiviral therapy of chronic hepatitis C. | Motokazu Mukaide Yasuhito Tanaka Hirokazu Kakuda Kei Fujiwara Fuat Kurbanov Eturo Orito Kentaro Yoshioka Kiyotaka Fujise Shoji Harada Takazumi Kozaki Kazuo Takemura Kazumasa Hikiji Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，4： | 3 |
| 2 | 中国内蒙自治区马饲育环境土壤中马红球菌的分布(英文)显示文摘对中国内蒙古自治区饲育马土壤环境中马红球菌的分布知之甚少;为此,在中国内蒙古通辽近郊、内蒙古南部锡林郭勒盟大草原、内蒙古东部呼伦贝尔大草原等马场共收集了108份土壤样品进行了马红球菌的检测。结果,锡林郭勒盟大草原和呼伦贝尔大草原的马红球菌分离率为25.9%~30.0%,通辽近郊土壤中分离率高达82.3%。这说明在通辽近郊马红球菌的分离菌数是草原土壤中马红球菌分离菌数的10倍。应用PCR技术检测了488株的毒力相关基因,相对分子质量分别为15000~17000(VapA)和20000(VapB)。所有的分离株都未检出毒力相关基因。这些无毒力分离株的质粒资料显示,各种大小的潜在质粒的发生率为13.3%~21.5%。本研究结果截然不同于我们新近对蒙古国的调查结论:马红球菌在蒙古国乌兰巴托的马群中不存在。造成这一差异的原因可能在于蒙古国的游牧生活和内蒙古的非游牧生活。 | 丁壮 Shinji TAKAI Hiroo MADARAME 常爽 黄海楠 霍晓伟 高明华 谭忠田 高双成 Fumiko HATORI Yukako SASAKI Tsutomu KAKUDA Shiro TSUBAKI | 2008 | 中国兽医学报2008，28，1： | 3 |
| 3 | Evaluation of a new histologic staging and grading system for primary biliary cirrhosis in comparison with classical systems显示文摘 | Yuko Kakuda Kenichi Harada Seiko Sawada-Kitamura Hiroko Ikeda Yasunori Sato Motoko Sasaki Hirofumi Okafuji Eishiro Mizukoshi Shuichi Terasaki Hajime Ohta Satomi Kasashima Atsuhiro Kawashima Yasuharu Kaizaki Shuichi Kaneko Yasuni Nakanuma | 2013 | Human Pathology2013，，6： | 2 |
| 4 | 施硅对稻田土壤氮矿化的影响(英文)显示文摘供试土壤分别采自日本山形县农业试验场 (土壤A)和鹤冈市吉田农户的水稻田 (土壤B)。将两种水稻土分别与硅酸钙和硅酸混合 ,在密闭条件下恒温培养 4周 ,测定施硅对土壤氮矿化的影响。在培养土壤中加入15N标记硫酸铵以评价施硅对加入土壤中氮去向的影响。结果表明 :在培养 1周内 ,施用硅酸钙 1g·kg- 1,2g·kg- 1与不施硅相比 ,土壤pH增加 0 .2~ 0 .3,NH4 + N总积累量分别增加 2 0 % ,2 6% (土壤A)和 9% ,11% (土壤B) ,土壤氮净矿化速率分别增加 2 6% ,35%(土壤A)和 13% ,13% (土壤B)。处理间差异在培养开始后的第 1周大于第 4周。硅酸对土壤pH及氮矿化速率没有影响。两种硅肥对施入土壤中氮的去向均无明显影响。研究结果揭示 ,硅酸钙促进土壤氮矿化的原因不是硅元素本身 。 | 符建荣 Ho Ando Kenichi Kakuda | 2001 | 浙江农业学报2001，13，3： | 2 |
| 5 | Effect of Milk Fat on the Stability of Vitamin A in Ultra-High Temperature Milk 显示文摘 | LAU B L T KAKUDA Y ARNOTT D R | 1986 | J Dairy Sci1986，69，： | 1 |
| 6 | Electrochemical performance of Al-doped LiMn2O4 prepared by different methods in solld-state reaction 显示文摘 | Teruaki Kakuda Kazuyoshi Uematsu Kenji Toda | 2007 | Journal of Power Sources2007，167，： | 1 |
| 7 | Expression and function of the Hnk-1 carbohydrate显示文摘 | Morita I Kizuka Y Kakuda S | 2008 | J Biochem2008，，143： | 1 |
| 8 | Optimal definition for PWI/DWI mismatch in acute ischemicstroke patients Journal of Cerebral Blood Flow&Metabolism显示文摘 | Wataru Kakuda Maarten G Lansherg Vincent N Thijs | 2008 | 28:8872008，28，： | 1 |
| 9 | Brain perfusion and upper limb motor function:A pilot study on the correlation between evolution of asymmetry in cerebral blood flow and improvement in FuglMeyer Assessment score after rTMS in chronic post-stroke patients显示文摘 | Takekawa T Kakuda W Uchiyama M | 2014 | J Neuroradiol2014，41，3： | 1 |
| 10 | Non-de-structive thermal property measurements of an APS TBCon an intact turbine blade 显示文摘 | KAKUDA T LIMARGA A VAIDYA A | 2010 | Surface & Coatings Tech-nology2010，205，44： | 1 |
| 11 | Comparison of the effects of high and low-frequency repetitive transeranial magnetic stimulation on upper limb hemiparesis in the ear- ly phase of stroke显示文摘 | Sasaki N Mizutani S Kakuda W | 2013 | J Stroke Cerebrovasc Dis2013，22，41： | 1 |
| 12 | Electrochemical performance of A1- doped LiMn204 prepared by different methods in solid-state reaction显示文摘 | Kakuda Teruaki Uematsu Kazuyoshi Toda Kenji | 2007 | Journal of Power Sources2007，167，： | 1 |
| 13 | Anti-spastic effect of low-frequency rTMS applied with occupational therapy in poststroke patients with upper limb hemipa- resis显示文摘 | Kakuda W Abo M Kobayashi K | 2011 | Brain Inj2011，25，5： | 1 |
| 14 | 查看详情显示文摘 | Susaki W Kakuda S Inada T Igawa T Tomioka A | | 0，，： | 1 |
| 15 | Hypoperfusion and vasoreactivity in the thalamus and cerebellum after stroke 显示文摘 | Sakashita Y Matsuda H Kakuda K | 1993 | Stroke1993，24，： | 1 |
| 16 | Short-term effect of pitavastatin treatment on glucose and lipid metabolism and oxidative stress in fasting and postprandial state using a test meal in Japanese men显示文摘 | Kakuda H Kobayashi J Nakato M | 2013 | Cholesterol2013，，31： | 1 |
| 17 | Effect of Storage Temperature on Age Gelation of Ultra High Temperature Milk Processed by Direct and Indirect Heating Systems显示文摘 | MANJI B KAKUDA Y ARNOTT D R | 1986 | J Dairy Sci1986，69，： | 1 |
| 18 | Highly Efficient and Versatile Acylation of Alcohols with Bi,(OTf)3 as Catalyst显示文摘 | ORITA A TANAHASHI C KAKUDA A | | 0，，16： | 1 |
| 19 | Comparison of the effects of high- and low-frequency repetitive transcrani- al magnetic stimulation on upper limb hemiparesis in the early phase of stroke显示文摘 | Sasaki N Mizutani S Kakuda W | 2013 | Stroke Cerebrovasc Dis2013，22，41： | 1 |
| 20 | Combined thera-peutic application of botulinum toxin type A,low-frequen-cy rTMS,and intensive occupational therapy for post-stroke spastic upper limb hemiparesis显示文摘 | Kakuda W Abo M Momosaki R | 2012 | Eur J Phys Re-habil Med2012，48，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员