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1Serum biomarker tests are useful in delineating between patients with gastric atrophy and normal,healthy stomach显示文摘AIM:To study the value of serum biomarker tests to differentiate between patients with healthy or diseased stomach mucosa:i.e.those with Helicobacter pylori(H pylori)gastritis or atrophic gastritis,who have a high risk of gastric cancer or peptic ulcer diseases.METHODS:Among 162 Japanese outpatients,pepsinogen-(Pg-)and(Pg)were measured using a conventional Japanese technique,and the European GastroPanel examination(Pg and Pg,gastrin-17 and H pylori antibodies).Gastroscopy with gastric biopsies was performed to classify the patients into those with healthy stomach mucosa,H pylori non-atrophic gastritis or atrophic gastritis.RESULTS:Pg-and Pg assays with the GastroPanel and the Japanese method showed a highly significant correlation.For methodological reasons,however,serum Pg-,but not Pg,was twice as high with the GastroPanel test as with the Japanese test.The biomarker assays revealed that 5%of subjects had advanced atrophic corpus gastritis which was also verified by endoscopic biopsies.GastroPanel examination revealed an additional seven patients who had either advanced atrophic gastritis limited to the antrum or antrum-predominant H pylori gastritis.When compared to the endoscopic biopsy findings,the GastroPanel examination classified the patients into groups with 'healthy' or 'diseased' stomach mucosa with 94% accuracy,95% sensitivity and 93% specifi city.CONCLUSION:Serum biomarker tests can be used to differentiate between subjects with healthy and diseased gastric mucosa with high accuracy.Katsunori Iijima Yasuhiko Abe Ryosuke Kikuchi Tomoyuki Koike Shuichi Ohara Pentti Sipponen Tooru Shimosegawa 2009World Journal of Gastroenterology2009,15,7:25
2Usefulness of noninvasive transient elastography for assessment of liver fibrosis stage in chronic hepatitis C显示文摘AIM:To evaluate the method of noninvasive transient elastography for assessment of histological stage of liver fibrosis in patients with chronic hepatitis C (CHC). METHODS: Two hundred and thirty-seven patients with CHC were included in this study. Liver biopsy was performed under ultrasonography on 217 of the patients, excluding twenty with clear clinical evidence of liver cirrhosis. Fifty subjects without liver disease were enrolled as a control group (stage 0). Twenty- five patients with sustained virological response (SVR) to interferon (IFN) therapy were also enrolled. These patients underwent liver biopsy before IFN therapy. Examination of liver stiffness (LS) was performed by elastography. RESULTS: Medians (50% levels) of LS were 4.1 (3.5-4.9), 6.3 (4.8-8.5), 8.8 (6.8-12.0), 14.6 (10.5-18.6), and 22.2 (15.4-28.0), respectively, in the fibrosis stages 0-4 (P < 0.001). LS was significantly correlated with four serum fibrosis markers. LS values in patients with SVR were 3.8 (3.5-5.6), 5.2 (4.4-6.8), 6.8 (6.1-7.6), and 6.1 (3.6-7.9), respectively, in the fibrosis stages 1-4. In all stages, LS for patients with SVR was significantly lower than that for patients who did not undergo IFN therapy. LS was significantly correlated with serum concentrations of hyaluronic acid, type Ⅳ collagen, type Ⅳ collagen 7S, and type Ⅲ procollagen N peptide. CONCLUSION: LS correlated well with the histologicalstage of fibrosis. Changes in liver fibrosis stage may thus be estimated noninvasively using transient elastography.Tadashi Takeda Takahiro Yasuda Yuji Nakayama Mika Nakaya Megumi Kimura Mariko Yamashita Ayumi Sawada Koji Abo Setsuko Takeda Hiroki Sakaguchi Susumu Shiomi Hitoshi Asai Shuichi Seki 2006World Journal of Gastroenterology2006,12,48:16
3Long-term outcome of percutaneous ethanol injection therapy for minimum-sized hepatocellular carcinoma显示文摘AIM: To evaluate long-term follow-up of minimum-sized hepatocellular carcinoma (HCC) treated with percutaneous ethanol injection (PEI). METHODS: PEI was applied to 42 lesions in 31 patients (23 male and eight female) with HCC < 15 mm in diameter, over the past 15 years. RESULTS: Overall survival rate was 74.1% at 3 years, 49.9% at 5 years, 27.2% at 7 years and 14.5% at 10 years. These results are superior to, or at least the same as those for hepatic resection and radiofrequency ablation. Survival was affected only by liver function, but not by sex, age, etiology of Hepatitis B virus or Hepatitis C virus, α-fetoprotein levels, arterial and portal blood flow, histological characteristics, and tumor multiplicity or size. Patients in Child-Pugh class A and B had 5-, 7- and 10-years survival rates of 76.0%, 42.2% and 15.8%, and 17.1%, 8.6% and 0%, respectively (P = 0.025). CONCLUSION: Treatment with PEI is best indicated for patients with HCC < 15 mm in Child-Pugh class A.Miyuki Taniguchi Soo Ryang Kim Susumu Imoto Hirotsugu Ikawa Kenji Ando Keiji Mita Shuichi Fuki Noriko Sasase Toshiyuki Matsuoka Masatoshi Kudo Yoshitake Hayashi 2008World Journal of Gastroenterology2008,14,13:16
4Effect of dietary docosahexaenoic acid on lipogenesis and lipolysis in black sea bream,Acanthopagrus schlegeli显示文摘Hatchery-reared juvenile black sea breams are characterized by a low level of highly unsaturated fatty acids in their bodies, as compared with wild fish. To assess the effect of docosahaxaenoic acid (DHA) on lipogenic and lipolysis enzymes, one-year fish were reared on a casein-based purified diet and a DHA fortified diet (1.5% DHA ethyl ester/kg diet) for 60 d, followed with a period of 55 d for starvation. Dietary DHA was effectively incorporated into the fish body. Fortification of DHA depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancreas and intraperitoneal fat body. Carnitine palmitoyltransferase as lipolysis enzyme in the hepatopancreas was active in the DHA fortified fish. Starvation after feeding experiment induced increased carnitine palmitoyltransferase activity in both control and DHA fortified fish and the activity remained higher in the DHA fortified fish, while the monoenes were selectively consumed prior to highly unsaturated fatty acids. These results indicated that dietary DHA depressed lipogenesis and activated lipolysis.JI Hong OM Ahamd Daud YOSHIMATSU Takao UMINO Testuya NAKAGAWA Heisuke FURUHASHI Makoto SAKAMOTO Shuichi 2007Acta Oceanologica Sinica2007,26,1:13
5Consequences of bullying victimization in childhood and adolescence:A systematic review and meta-analysis显示文摘AIM To identify health and psychosocial problems associated with bullying victimization and conduct a meta-analysis summarizing the causal evidence.METHODS A systematic review was conducted using Pub Med, EMBASE, ERIC and Psyc INFO electronic databases up to 28 February 2015. The study included published longitudinal and cross-sectional articles that examined health and psychosocial consequences of bullying victimization. All meta-analyses were based on qualityeffects models. Evidence for causality was assessed using Bradford Hill criteria and the grading system developed by the World Cancer Research Fund.RESULTS Out of 317 articles assessed for eligibility, 165 satisfied the predetermined inclusion criteria for meta-analysis.Statistically significant associations were observed between bullying victimization and a wide range of adverse health and psychosocial problems. The evidence was strongest for causal associations between bullying victimization and mental health problems such as depression, anxiety, poor general health and suicidal ideation and behaviours. Probable causal associations existed between bullying victimization and tobacco and illicit drug use. CONCLUSION Strong evidence exists for a causal relationship between bullying victimization, mental health problems and substance use. Evidence also exists for associations between bullying victimization and other adverse health and psychosocial problems, however, there is insufficient evidence to conclude causality. The strong evidence that bullying victimization is causative of mental illness highlights the need for schools to implement effective interventions to address bullying behaviours.Sophie E Moore Rosana E Norman Shuichi Suetani Hannah J Thomas Peter D Sly James G Scott 2017World Journal of Psychiatry2017,7,1:12
6Isolation of a Novel Lodging Resistance QTL Gene Involved in Strigolactone Signaling and Its Pyramiding with a QTL Gene Involved in Another Mechanism显示文摘住宿是到达到增加的庄稼生产率的一个主要障碍。在一次尝试到在米饭为灰煤杆力量理解机制,我们孤立一个有效量的特点地点( QTL ),强壮的 CULM3 ( SCM3 ),哪个与米饭 TEOSINTE BRANCHED1 ( OsTB1 )相同的原因的基因,基因以前报导了断然控制发信号的 strigolactone ( SL )。带 SCM3 的一根 near-isogenic 线(无) 证明尽管有期望的减少,提高的灰煤杆力量和增加的小穗状花小穗在 tiller 数字数,显示 SL 也有在提高灰煤杆力量和小穗状花小穗数字的一个积极角色。我们生产了带 SCM3 和 SCM2 的一根节节上升的线,编码涉及圆锥花序开发的 APO1 的另一 QTL。NIL-SCM2+SCM3 比 NIL-SCM2 和 NIL-SCM3 和这些 QTL 的添加剂效果引起的一个增加的小穗状花小穗数字显示出强壮得多的灰煤杆。我们讨论没有为繁殖导致有害特点,利用这些强壮的灰煤杆 QTL 的合适的等位基因的重要性。Kenji Yano Taiichiro Ookawa Koichiro Aya Yusuke Ochiai Tadashi Hirasawa Takeshi Ebitani Takeshi Takarada Masahiro Yano Toshio Yamamoto Shuichi Fukuoka Jianzhong Wu Tsuyu Ando Reynante Lacsamana Ordonio Ko Hirano Makoto Matsuoka 2015Molecular Plant2015,8,2:12
7Hepatitis B virus X protein induces hepatic stem cell-like features in hepatocellular carcinoma by activating KDM5B显示文摘AIM To determine the role of hepatitis B virus X protein(HBx), HBx in regulating hepatic progenitor cell(HPC)-like features in hepatocellular carcinoma(HCC) and the underlying molecular mechanisms.METHODS We used a retrovirus vector to introduce wild type HBx or empty vector into Hep G2 cells. We then used these cells to analyze cell proliferation, senescence, transformation, and stem-like features. Gene expression profiling was carried out on Affymetrix GeneC hip Human U133A2.0 ver.2 arrays according to the manufacturer's protocol. Unsupervised hierarchical clustering analysis and Class Comparison analysis were performed by BRB-Array Tools software Version 4.2.2. A total of 238 hepatitis B virus(HBV)-related HCC patients' array data were used for analyzing clinical features.RESULTS The histone demethylase KDM5 B was significantlyhighly expressed in HBV-related HCC cases(P < 0.01). In HBV proteins, only HBx up-regulated KDM5 B by activating c-myc. Hepatic stem cell(Hp SC) markers(EpC AM, AFP, PROM1, and NANOG) were significantly highly expressed in KDM5B-high HCC cases(P < 0.01). KDM5 B played an important role in maintaining HpS Clike features and was associated with a poor prognosis. Moreover, inhibition of KDM5 B suppressed spheroid formation and cell invasion in vitro.CONCLUSION HBx activates the histone demethylase KDM5 B and induces HPC-like features in HCC. Histone demethylases KDM5 B may be an important therapeutic target against HBV-related HCC cases.Xuyang Wang Naoki Oishi Tetsuro Shimakami Taro Yamashita Masao Honda Seishi Murakami Shuichi Kaneko 2017World Journal of Gastroenterology2017,23,18:10
8Endovascular technique using a snare and suture for retrieving a migrated peripherally inserted central catheter in the left pulmonary artery显示文摘We report a successful endovascular technique using a snare with a suture for retrieving a migrated broken peripherally inserted central catheter(PICC)in a chemotherapy patient.A 62-year-old male received monthly chemotherapy through a central venous port implanted into his right subclavian area.The patient completed chemotherapy without complications 1 mo ago;however,he experienced pain in the right subclavian area during his last chemotherapy session.Computed tomography on that day showed migration of a broken PICC in his left pulmonary artery,for which the patient was admitted to our hospital.We attempted to retrieve the ectopic PICC through the right jugular vein using a gooseneck snare,but were unsuccessful because the catheter was lodged in the pulmonary artery wall.Therefore,a second attempt was made through the right femoral vein using a snare with triple loops,but we could not grasp the migrated PICC.Finally,a string was tied to thetop of the snare,allowing us to curve the snare toward the pulmonary artery by pulling the string.Finally,the catheter body was grasped and retrieved.The endovascular suture technique is occasionally extremely useful and should be considered by interventional cardiologists for retrieving migrated catheters.Hiroki Teragawa Takashi Sueda Yuichi Fujii Hiroaki Takemoto Yasushi Toyota Shuichi Nomura Keigo Nakagawa 2013World Journal of Cardiology2013,5,9:9
9Promising biological therapies for ulcerative colitis: A review of the literature显示文摘Ulcerative colitis(UC) is a chronic lifelong condition characterized by alternating flare-ups and remission. There is no single known unifying cause, and the pathogenesis is multifactorial, with genetics, environmental factors, microbiota, and the immune system all playing roles. Current treatment modalities for UC include 5-aminosalicylates, corticosteroids, immunosuppressants(including purine antimetabolites, cyclosporine, and tacrolimus), and surgery. Therapeutic goals for UC are evolving. Medical treatment aims to induce remission and prevent relapse of disease activity. Infliximab, an anti-tumor necrosis factor(TNF)-α monoclonal antibody, is the first biological agent for the treatment of UC. Over the last decade, infliximab and adalimumab(anti-TNF-α agents) have been used for moderate to severe UC, and have been shown to be effective in inducing and maintaining remission. Recent studies have indicated that golimumab(another anti-TNF-α agent), tofacitinib(a Janus kinase inhibitor), and vedolizumab and etrolizumab(integrin antagonists), achieved good clinical remission and response rates in UC. Recently, golimumab and vedolizumab have been approved for UC by the United States Food and Drug Administration. Vedolizumab may be used as a first-line alternative to anti-TNF-α therapy in patients with an inadequate response to corticosteroids and/or immunosuppressants. Here, we provide updated information on various biological agents in the treatment of UC.Hirotada Akiho Azusa Yokoyama Shuichi Abe Yuichi Nakazono Masatoshi Murakami Yoshihiro Otsuka Kyoko Fukawa Mitsuru Esaki Yusuke Niina Haruei Ogino 2015World Journal of Gastrointestinal Pathophysiology2015,6,4:8
10Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial显示文摘Kazutoshi Fukase Mototsugu Kato Shogo Kikuchi Kazuhiko Inoue Naomi Uemura Shiro Okamoto Shuichi Terao Kenji Amagai Shunji Hayashi Masahiro Asaka 2008The Lancet2008,,9636:7
11正烷基葡糖苷、甘露糖苷和半乳糖苷的表面活性、生物降解性和抗菌性显示文摘各种烷基糖苷都有其重要性,一是因为它们在生态学和医学上的应用潜力,二是因为它们能由天然可再生的原料(脂肪醇和糖)制备。而且,从生态学和能源观点考虑,烷基糖苷将是下一代新型表面活性剂最有希望的品种之一。烷基糖苷的制备和物理性质已有报道,但大都集中讨论烷基葡糖苷,而对其他的烷基糖苷则报道的不多。最近,正辛基β-Shuichi Matsumura 于珍祥 1992日用化学工业译丛1992,,3:7
12Biochemically curative surgery for gastrinoma in multiple endocrine neoplasia type 1 patients显示文摘AIM: To search for the optimal surgery for gastrinoma and duodenopancreatic neuroendocrine tumors in patients with multiple endocrine neoplasia type 1. METHODS: Sixteen patients with genetically confirmed multiple endocrine neoplasia type 1 (MEN 1) and Zollinger-Ellison syndrome (ZES) underwent resection of both gastrinomas and duodenopancreatic neuroendocrine tumors (NETs) between 1991 and 2009. For localization of gastrinoma, selective arterial secretagogue injection test (SASI test) with secretin or calcium solution was performed as well as somatostatin receptor scintigraphy (SRS) and other imaging methods such as computed tomography (CT) or magnetic resonance imaging (MRI). The modus of surgery for gastrinoma has been changed over time, searching for the optimal surgery: pancreaticoduodenectomy (PD) was first performed guided by localization with the SAST test, then local resection of duodenal gastrinomas with dissection of regional lymph nodes (LR), and recently pancreas-preserving total duodenectomy (PPTD) has been performed for multiple duodenal gastrinomas. RESULTS: Among various types of preoperative localizing methods for gastrinoma, the SASI test was the most useful method. Imaging methods such as SRS or CT made it essentially impossible to differentiate functioning gastrinoma among various kinds of NETs. However, recent imaging methods including SRS or CT were useful for detecting both distant metastases and ectopic NETs; therefore they are indispensable for staging of NETs. Biochemical cure of gastrinoma was achieved in 14 of 16 patients (87.5%); that is, 100% in 3 patients who underwent PD, 100% in 6 patients who underwent LR (although in 2 patients (33.3%) second LR was performed for recurrence of duodenal gastrinoma), and 71.4% in 7 patients who underwent PPTD. Pancreatic NETs more than 1 cm in diameter were resected either by distal pancreatectomy or enucleations, and no hepatic metastases have developed postoperatively. Pathological study of the resected specimens revealed co-existence of pancreatic gastrinoma with duodenal gastrinoma in 2 of 16 patients (13%), and G cell hyperplasia and/or microgastrinoma in the duodenal Brunner's gland was revealed in all of 7 duodenal specimens after PPTD. CONCLUSION: Aggressive resection surgery based on accurate localization with the SASI test was useful for biochemical cure of gastrinoma in patients with MEN 1.Masayuki Imamura Izumi Komoto Shuichi Ota Takuya Hiratsuka Shinji Kosugi Ryuichiro Doi Masaaki Awane Naoya Inoue 2011World Journal of Gastroenterology2011,17,10:5
13Phase III study of sorafenib after transarterial chemoembolisation in Japanese and Korean patients with unresectable hepatocellular carcinoma显示文摘Masatoshi Kudo Kazuho Imanaka Nobuyuki Chida Kohei Nakachi Won-Young Tak Tadatoshi Takayama Jung-Hwan Yoon Takeshi Hori Hiromitsu Kumada Norio Hayashi Shuichi Kaneko Hirohito Tsubouchi Dong Jin Suh Junji Furuse Takuji Okusaka Katsuaki Tanaka Osamu Matsui 2011European Journal of Cancer2011,,14:5
14Long-term survival of a case with multiple liver metastases from duodenal gastrointestinal stromal tumor drastically reduced by the treatment with imatinib and hepatectomy显示文摘工具包受体酷氨酸激酶的组成的激活是在胃肠的基质肿瘤(大意) 的致病的一个关键因素。但是几乎没有 imatinib mesylate (IM ) 和外科疗法的联合是否能与 unresectable 在情况中延长幸存的很少信息多重肝转移。我们报导酷氨酸激酶禁止者 IM 和外科疗法对待的大意的手术后的复发的一个案例。对处理的起始的完全的反应(CR ) 为 18 瞬间继续,但是单个肝转移在 IM 处理期间在左肝的脑叶显示出生长。在再发性瘤的部分切除术以后,手术后的路线是平静的,没有复发,病人为 24 瞬间幸存。当前, imatinib 是为 non-resectable 大意的首要的治疗,但是单个代理人治疗经常导致肿瘤抵抗。就算到 imatinib 的忍耐发生, imatinib 和外科疗法的联合能在这里报导了的一些情况中延长幸存。然而,周期性的大意的很多案例上的进一步的研究是必要的评估治疗与外科相结合的 IM 的有效性。Chouhei Sakakura Akeo Hagiwara Koji Soga Koji Miyagawa Susumu Nakashima Tetsuji Yoshikawa Shuichi Kin Yuenn Nakase Nobuki Yamaoka Yoshihiko Sagara Hisakazu Yamagishi 2006World Journal of Gastroenterology2006,12,17:5
15Effects of age on survival and morbidity in gastric cancer patients undergoing gastrectomy显示文摘AIM To evaluate clinicopathological features and surgical outcomes of gastric cancer in elderly and non-elderly patients after inverse probability of treatment weighting(IPTW) method using propensity score.METHODS We enrolled a total of 448 patients with histologically confirmed primary gastric carcinoma who received gastrectomies. Of these, 115 patients were aged > 80 years old(Group A), and 333 patients were aged < 79 years old(Group B). We compared the surgical outcomes and survival of the two groups after IPTW.RESULTS Postoperative complications, especially respiratory complications and hospital deaths, were significantly more common in Group A than in Group B(P < 0.05). Overall survival(OS) was significantly lower in Group A patients than in Group B patients. Among the subset of patients who had pathological Stage Ⅰ?disease, OS was significantly lower in Group A(P < 0.05) than Group B, whereas cause-specific survival was almost equal in the two groups. In multivariate analysis, pathological stage, histology, and extent of lymph node dissection wereindependent prognostic values for OS.CONCLUSION When the gastrectomy was performed in gastric cancer patients, we should recognized high mortality and comorbidities in that of elderly. More extensive lymph node dissection might improve prognoses of elderly gastric cancer patients.Yoshinori Fujiwara Shuichi Fukuda Masanori Tsujie Hajime Ishikawa Kotaro Kitani Keisuke Inoue Masao Yukawa Masatoshi Inoue 2017World Journal of Gastrointestinal Oncology2017,9,6:4
16Ramucirumab plus paclitaxel versus placebo plus paclitaxel in patients with previously treated advanced gastric or gastro-oesophageal junction adenocarcinoma (RAINBOW): a double-blind, randomised phase 3 trial显示文摘Hansjochen Wilke Kei Muro Eric Van Cutsem Sang-Cheul Oh Gy?rgy Bodoky Yasuhiro Shimada Shuichi Hironaka Naotoshi Sugimoto Oleg Lipatov Tae-You Kim David Cunningham Philippe Rougier Yoshito Komatsu Jaffer Ajani Michael Emig Roberto Carlesi David Ferry Kuma 2014Lancet Oncology2014,,11:4
17Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial显示文摘Kazutoshi Fukase Mototsugu Kato Shogo Kikuchi Kazuhiko Inoue Naomi Uemura Shiro Okamoto Shuichi Terao Kenji Amagai Shunji Hayashi Masahiro Asaka 2008The Lancet . 2008 (9636)2008,,:4
18Significance of hyperuricemia as a risk factor for developing ESRD in a screened cohort显示文摘Kunitoshi Iseki Yoshiharu Ikemiya Taku Inoue Chiho Iseki Kozen Kinjo Shuichi Takishita 2004American Journal of Kidney Diseases2004,,4:4
19Multistep hepatocarcinogenesis from a dysplastic nodule to well-differentiated hepatocellular carcinoma in a patient with alcohol-related liver cirrhosis显示文摘We describe a rare case of the transformation of a dysplastic nodule into well-differentiated hepato- cellular carcinoma (HCC) in a 56-year-old man with alcoholrelated liver cirrhosis. Ultrasound (US) disclosed a 10 mm hypoechoic nodule and contrast enhanced US revealed a hypovascular nodule, both in segment seven. US-guided biopsy revealed a high-grade dysplastic nodule characterized by enhanced cellularity with a high N/C ratio, increased cytoplasmic eosinophilia, and slight cell atypia. One year later, the US pattern of the nodule changed from hypoechoic to hyperechoic without any change in size or hypovascularity. US-guided biopsy revealed well-differentiated HCC of the same features as shown in the first biopsy, but with additional pseudoglandular formation and moderate cell atypia. Moreover, immunohistochemical staining of cyclase- associated protein 2, a new molecular marker of well- differentiated HCC, turned positive. This is the first case of multistep hepatocarcinogenesis from a dysplastic nodule to well-differentiated HCC within one year in alcohol-related liver cirrhosis.Soo Ryang Kim Hirotsugu Ikawa Kenji Ando Keiji Mita Shuichi Fuki Michiie Sakamoto Yoshihiro Kanbara Toshiyuki Matsuoka Masatoshi Kudo Yoshitake Hayashi 2007World Journal of Gastroenterology2007,13,8:4
20miR-218 on the genomic loss region of chromosome 4p15.31 functions asa tumor suppressor in bladder cancer显示文摘Shuichi Tatarano Takeshi Chiyomaru Kazumori Kawakami Hideki Enokida Hirofumi Yoshino Hideo Hidaka Takeshi Yamasaki Kazuya Kawahara Kenryu Nishiyama Naohiko Seki Masayuki Nakagawa 2011International Journal of Oncology2011,,1:3
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