| 3 | Lipin基因表达与宫内发育迟缓大鼠肝脏脂肪含量的相关性研究显示文摘目的 探讨宫内发育迟缓(intrauterine growth retardation,IUGR)大鼠肝脏Lipin2基因和内脏脂肪组织Lipin1基因的表达与肝脏脂肪含量的相关性。方法 使用母孕期低蛋白(10%蛋白)饮食法喂养孕鼠制造IUGR仔鼠模型,对照组孕鼠在孕期使用正常蛋白饲料喂养(蛋白含量21%)。分别在两组仔鼠生后1 d、1周、3周、8周和12周时称体重并留取仔鼠的肝脏组织,在生后3周、8周和12周留取两组仔鼠的内脏脂肪组织。采用3.0T氢质子磁共振波谱法检测两组大鼠生后3周、8周、12周时肝脏脂肪含量;采用Real-time PCR法检测两组大鼠各时间点肝脏组织Lipin2、内脏脂肪组织Lipin1基因的mRNA表达水平;采用Western blot法检测两组大鼠肝脏组织Lipin2、内脏脂肪组织Lipin1蛋白表达水平。采用Pearson相关分析Lipin mRNA及其蛋白表达与肝脏脂肪含量的相关性。结果 生后3周、8周、12周时,IUGR组仔鼠内脏脂肪组织Lipin1 mRNA及其蛋白表达水平均高于对照组(P<0.05)。生后1 d时IUGR组肝脏组织Lipin2 mRNA及其蛋白表达水平低于对照组(P<0.05),而生后1周、3周、8周、12周时Lipin2 mRNA及其蛋白表达水平均高于对照组(P<0.05)。生后3周时IUGR仔鼠和对照组肝脏脂肪含量比较差异无统计意义(P>0.05),生后8周、12周时IUGR组仔鼠肝脏脂肪含量显著高于对照组(P<0.05)。Lipin1蛋白和mRNA表达与肝脏脂肪含量呈正相关(分别r=0.628、0.521,P<0.05),Lipin2蛋白和mRNA表达与脂肪含量呈正相关(分别r=0.601、0.524,P<0.05)。结论 IUGR大鼠内脏脂肪组织Lipin1和肝脏组织Lipin2 mRNA及其蛋白表达上调可引起肝脏脂肪含量增加,可能与导致IUGR大鼠成年期肥胖有关。 | 卞京 陈平洋 卞读军 贺晓日 Alpha Kalonda Mutamba 王涛 | 2022 | 中国当代儿科杂志2022，24，4： | 1 |
| 4 | Assessment of Trace Metal Levels of Groundwater in Lubumbashi,Kampemba and Kamalondo Communes of Lubumbashi City,Democratic Republic of Congo显示文摘Trace metal levels of groundwater in Lubumbashi,Kampemba and Kamalondo communes of Lubumbashi city were assessed from October 2016 to February 2017.Two hundred forty water samples collected from twenty-two spade-sunk wells and twelve drilled wells in these three communes of Lubumbashi city were analyzed for their metal contents using ICP-SF-MS(Inductively Coupled Plasma-Sector Field Mass Spectrometry).Twenty trace elements including strontium,molybdenum,cadmium,cesium,barium,tungsten,thallium,lead,bismuth,uranium,aluminum,vanadium,chromium,manganese,iron,cobalt,nickel,copper,zinc and arsenic were recorded at varying concentrations in all the water samples and were compared with the WHO(World Health Organization),US EPA(Environmental Protection Agency)and EU(European Union)drinking water MCLs(Maximum Concentration Limits)for cadmium,barium,thallium,lead,uranium,aluminum,chromium,manganese,iron,nickel,copper,zinc and arsenic.Mean cadmium,lead,aluminum,manganese,iron,nickel,zinc and arsenic levels respectively exceeded the WHO,US EPA and EU drinking water MCLs in 6.66%,3.38%,26.67%,5.02%,30.03%,3.38%,1.64% and 5.02% of the samples with the highest mean levels of 116.89μg/L for cadmium,38.162μg/L for lead,2,712.5μg/L for aluminum,1,242.68μg/L for manganese,17,325.98μg/L for iron,64.647μg/L for nickel,9,900.72μg/L for zinc and 65.458μg/L for arsenic.Mean water pH values ranged from 4.7 to 11.1 with 19.17% of the groundwater samples having mean pH values outside the WHO drinking water pH optimum range values of 6.5-8.5,including 5.02% of the water samples which were acidic(mean pH values ranging from 4.7 to 6.4)and 14.15% which were alkaline(mean pH values ranging from 8.6 to 11.1).With such physicochemical and trace metal contamination status of groundwater in the three communes of Lubumbashi city,there is a high risk to the health of people who use that water to meet their drinking water needs. | Bamba Bukengu Muhaya Sonia Catherine Mulongo Clarisse Zoza Kunyonga Alexis Woot Mpomanga Emery Mutombo Kalonda | 2021 | Journal of Environmental Science and Engineering(A)2021，10，1： | 0 |
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