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| 1 | A review of isolation methods, structure features and bioactivities of polysaccharides from Dendrobium species显示文摘Dendrobium,orchid,is a traditional Chinese herb medicine applied extensively as tonic and precious food for thousands of years recorded in ancient Chinese medical book'Shen Nong’s Materia Medica'.It’s well known that bioactivities are usually related to the ingredients’basis.Based on the previous research,Dendrobium species contain amino acid,sesquiterpenoids,alkaloids and polysaccharides.As the bioactive substances,carbohydrate shows extensive activities in antitumor,antiglycation,immune-enhancing,antivirus,antioxidant,antitumor and etc.Therefore,as the main biologically active substance,the exact structures and latent activities of polysaccharides from Dendrobium species are widely focused on.In this review,we focus on the advancements of extraction methods and diversity of structures and bioactivities of polysaccharides obtained from Dendrobium species. | YUE Han ZENG Hui DING Kan | 2020 | Chinese Journal of Natural Medicines2020，18，1： | 20 |
| 2 | Research advances in the treatment of Alzheimer's disease with polysaccharides from traditional Chinese medicine显示文摘Alzheimer's disease(AD) is a neurodegenerative disorder characterized by the loss of patients' memory and their cognitive abilities and the mechanism is not completely clear. Although a variety of drugs have been approved for the AD treatment, substances which can prevent and cure AD are still in great need. The effect of polysaccharides from traditional Chinese medicine(TCM) on anti-AD has gained great progress and attained more and more attention in recent years. In this review, research advances in TCM-polysaccharides on AD made in this decade are summarized. | LIU Qin WANG Shun-Chun DING Kan | 2017 | Chinese Journal of Natural Medicines2017，15，9： | 12 |
| 3 | Improved corrosion resistance and thermal stability of sintered Nd-Fe-B magnets with holmium substitution显示文摘The effects of Ho substitution for Nd on the microstructure, corrosion resistance and thermal stability of the Nd-Fe-B magnets were investigated. The(Nd,Ho)-O phase was formed with increasing Ho substitution. The results of potentiodynamic polarization and highly accelerated stress test show improved corrosion resistance with increasing Ho substitution. The optimum mass loss 0.29 mg/cm^2 is achieved.Moreover, the average temperature coefficients for remanence and coercivity in the range of 25-150℃are both closer to zero, indicating improved thermal stability. The mechanisms for the improved corrosion resistance and thermal stability are discussed in relation to the microstructure featuring the(Nd,Ho)-O phase. | Jinghui Di Shuai Guo Ling Chen Pengpeng Yi Guangfei Ding Kan Chen Ming Li Don Lee Aru Yan | 2018 | Journal of Rare Earths2018，36，8： | 11 |
| 4 | Cordycepin inhibits pancreatic cancer cell growth in vitro and in vivo via targeting FGFR2 and blocking ERK signaling显示文摘Cordycepin(3′-deoxyadenosine) from Cordyceps militaris has been reported to have anti-tumor effects. However, the molecular target and mechanism underlying cordycepin impeding pancreatic cancer cell growth in vitro and in vivo remain vague. In this study, we reported functional target molecule of cordycepin which inhibited pancreatic cancer cells growth in vitro and in vivo.Cordycepin was confirmed to induce apoptosis by activating caspase-3, caspase-9 and cytochrome c. Further studies suggested that MAPK pathway was blocked by cordycepin via inhibiting the expression of Ras and the phosphorylation of Erk. Moreover, cordycepin caused S-phase arrest and DNA damage associated with activating Chk2(checkpoint kinase 2) pathway and downregulating cyclin A2 and CDK2 phosphorylation. Very interestingly, we showed that cordycepin could bind to FGFR2(KD = 7.77 × 10-9) very potently to inhibit pancreatic cancer cells growth by blocking Ras/Er K pathway. These results suggest that cordycepin could potentially be a leading compound which targeted FGFR2 to inhibit pancreatic cells growth by inducing cell apoptosis and causing cell cycle arrest via blocking FGFR/Ras/ERK signaling for anti-pancreatic cancer new drug development. | LI Xue-Ying TAO Hong JIN Can DU Zhen-Yun LIAO Wen-Feng TANG Qing-Jiu DING Kan | 2020 | Chinese Journal of Natural Medicines2020，18，5： | 10 |
| 5 | 3D C_f/SiBCN composites prepared by an improved polymer infiltration and pyrolysis显示文摘Using liquid poly(methylvinyl)borosilazanes(PMVBSZ)as precursor,carbon fiber reinforced SiBCN matrix composites(C_f/SiBCN)were fabricated by a modified polymer infiltration and pyrolysis(PIP)process.With dicumyl peroxide added as cross-linking agent,the PMVBSZ could be solidified at a low temperature of 120℃,leading to a high ceramic yield of~70%.The cross-linking mechanism and ceramization processes of the precursor were investigated in detail.Moreover,a modified infiltration technology was developed,which improved the efficiency and protected the precursor against moist air during PIP.Consequently,the obtained C_f/SiBCN composites had an oxygen content of around 1.22 wt%.Benefiting from the high ceramic yield and high efficiency of the modified PIP,C_f/SiBCN composites with an open porosity of~10%and uniform microstructure were obtained after only 7 cycles of PIP.The flexural strength and fracture toughness of the derived C_f/SiBCN composites were 371 MPa and 12.9 MPa·m^(1/2),respectively.This work provides a potential route for the fabrication of high performance C_f/SiBCN composites. | Qi DING Dewei NI Zhen WANG Hao ZHONG Hongda WANG Haijun ZHOU Yanmei KAN Shaoming DONG | 2018 | Journal of Advanced Ceramics2018，7，3： | 9 |
| 6 | Diluted ferromagnetic semiconductor(LaCa)(ZnMn)SbO isostructural to “1111” type iron pnictide superconductors显示文摘We report discovery of ferromagnetism in(LaCa)(ZnMn)SbO isostructural to the well-studied iron-based superconductor LaFeAs(O1 xFx).Spin is induced by partial substitution of Mn2+for Zn2+,while charge is induced by substitution of Ca2+for La3+within the parent compound LaZnSbO.Ferromagnetism with Curie temperature(TC)is observed up to 40 K at the spin doping 0.15 by introducing Mn2+into the Zn2+sites for(La0.95Ca0.05)(Zn1 xMnx)SbO.The Hall coefficient measurement indicates p-type carrier for(La0.95Ca0.05)(Zn0.9Mn0.1)SbO with concentration of n^1020cm 3showing anomalous Hall effect below TC. | HAN Wei ZHAO Kan WANG XianCheng LIU QingQing NING FanLong DENG Zheng LIU Ying ZHU JinLong DING Cui MAN HuiYuan JIN ChangQing | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，11： | 8 |
| 7 | Promoted effect of zinc–nickel bimetallic oxides supported on HZSM-5 catalysts in aromatization of methanol显示文摘Zn/ZSM-5(NZ2) and Zn/Ni/ZSM-5(NZ3) as the catalysts for methanol to aromatics(MTA) were synthesized by a simple ultrasonic impregnation. The textural and acid properties of all catalysts were characterized using XRD, HRTEM, NH_3-TPD, Py-IR, XPS, XRF and TG techniques. The XRD and HRTEM results showed that the basic zeolite structures were not affected much with the incorporation of Zn and Ni species. However, great changes have taken place in acid properties. The Py-IR and XPS results indicated that the Zn-Lewis acid sites(ZnOH^+ species), which have stronger interaction with the zeolite framework compared with ZnO species, were generated at the expense of B acid sites with the incorporation of zinc species. Moreover, the product analysis results showed that the incorporation of zinc species promoted the primary aromatization by enhancing the dehydroaromatization and suppressing the cracking and subsequent H-transfer reaction. Furthermore, the addition of Ni species well inhibited the loss of zinc species by converting partial ZnO species to ZnOH^+ species, and thus improved the aromatization activity and catalyst stability. The catalytic performance results showed that the NZ3 possess higher conversion of methanol in a longer time and lower average rate of coke formation compared with NZ2. In addition,the NZ3 also exhibited the highest yield of BTX as the reaction proceeds. | Yanming Jia Junwen Wang Kan Zhang Wei Feng Shibin Liu Chuanmin Ding Ping Liu | 2017 | Journal of Energy Chemistry2017，26，3： | 7 |
| 8 | WSS25, a sulfated polysaccharide, inhibits RANKL-induced mouse osteoclast formation by blocking SMAD/ID1 signaling显示文摘目的: WSS25 是从 Gastrodia elata 双性人的根茎提取的 sulfated 多糖,它被发现绑在骨头形态基因蛋白质 2 (BMP-2 ) 在 hepatocellular 癌症房间。因为 BMP-2 可以调整骨破折和造骨细胞,这里,我们在 ovariectomized 老鼠在 vitro 和骨头损失在 osteoclastogenesis 上调查了 WSS25 的效果。 | Cheng CHEN Yi QIN Jian-ping FANG Xin-yan NI Jian YAO Hai-ying WANG Kan DING | 2015 | Acta Pharmacologica Sinica2015，36，9： | 6 |
| 9 | Effi cacy and safety of low-dose corticosteroids for acute respiratory distress syndrome:A systematic review and meta-analysis显示文摘BACKGROUND:There are confl icting results regarding whether corticosteroids have better effi cacy than placebo in acute respiratory distress syndrome(ARDS)patients.Therefore,we aim to further evaluate the effi cacy and safety of corticosteroids in adult ARDS patients.METHODS:The databases,including Medline,EMBASE,and Cochrane Central Register of Controlled Trials(CENTRAL)in the Cochrane Library,were searched from their inception to May 2,2020.Randomized controlled trials(RCTs)and observational cohort studies were selected to assess the use of corticosteroids in adult ARDS patients.The quality of the results was judged by the Grading of Recommendations Assessment,Development,and Evaluation(GRADE)methodology.The inverse-variance method with random or fixed effects modeling was used to compute pooled odds ratio(OR),standardized mean diff erence(SMD),and their 95%confi dence interval(CI).RESULTS:Eight eligible RCTs and six cohort studies were included.The use of corticosteroids was associated with reduced mortality(OR 0.57,95%CI 0.43-0.76,I2=35.1%,P=0.148)in ARDS patients,and the result was confirmed in the included cohort studies(OR 0.51,95%CI 0.27-0.95,I2=66.7%,P=0.010).The subgroup analysis stratified by the initiation time and duration of corticosteroid use showed that early ARDS and prolonged corticosteroid use had signifi cant survival benefits in the RCTs.The low-dose corticosteroid use was also associated with significantly more ventilator-free days and a reduced rate of new infections in ARDS patients.CONCLUSIONS:The low-dose corticosteroid therapy may be safe and reduce mortality,especially in patients with prolonged treatment and early ARDS. | Yu-qing Cui Xian-fei Ding Huo-yan Liang Dong Wang Xiao-juan Zhang Li-feng Li Quan-cheng Kan Le-xin Wang Tong-wen Sun | 2021 | World Journal of Emergency Medicine2021，12，3： | 6 |
| 10 | Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice显示文摘Polysaccharides are widely present in herbs with multiple activities,especially immunity regulation and metabolic benefits for metabolic disorders.However,the underlying mechanisms are not well under-stood.Functional metabolomics is increasingly used to investigate systemic effects on the host by iden-tifying metabolites with particular functions.This study explores the mechanisms underlying the metabolic benefits of Astragalus polysaccharides(APS)by adopting a functional metabolomics strategy.The effects of APS were determined in eight-week high-fat diet(HFD)-fed obese mice.Then,gas chromatography–time-of-flight mass spectrometry(GC–TOFMS)-based untargeted metabolomics was performed for an analysis of serum and liver tissues,and liquid chromatography–tandem mass spectrom-etry(LC–MS/MS)-based targeted metabolomics was performed.The potential functions of the metabo-lites were tested with in vitro and in vivo models of metabolic disorders.Our results first confirmed the metabolic benefits of APS in obese mice.Then,metabolomics analysis revealed that APS supplemen-tation reversed the HFD-induced metabolic changes,and identified 2-hydroxybutyric acid(2-HB)as a potential functional metabolite for APS activity that was significantly decreased by a HFD and reversed by APS.Further study indicated that 2-HB inhibited oleic acid(OA)-induced triglyceride(TG)accumula-tion.It was also found to stimulate the expression of proteins in lipid degradation in hepatocytes and TG lipolysis in 3T3-L1 cells.Moreover,it was found to reduce serum TG and regulate the proteins involved in lipid degradation in high-fat and high-sucrose(HFHS)-fed mice.In conclusion,our study demonstrates that the metabolic benefits of APS are at least partially due to 2-HB generation,which modulated lipid metabolism both in vitro and in vivo.Our results also highlight that functional metabolomics is practical for investigating the mechanism underlying the systemic benefits of plant polysaccharides. | Bingbing Li Ying Hong Yu Gu Shengjie Ye Kaili Hu Jian Yao Kan Ding Aihua Zhao Wei Jia Houkai Li | 2022 | Engineering2022，8，2： | 6 |
| 11 | A novel small molecule, HK-156, inhibits lipopoly- saccharide-induced activation of NF-KB signaling and improves survival in mouse models of sepsis显示文摘 | Jian-ping FANG Yang LIU Jie LI Wen-feng LIAO You-hong HU Kan DING | 2012 | Acta Pharmacologica Sinica2012，33，9： | 6 |
| 12 | Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS显示文摘In this study,seven alkaloids were detected in Wu-tou decoction using ultra performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS').The aim of this study was to investigate the effect of Fritillariae Cirrhosae Bulbus,Fritillariae Thunbergii Bulbils,Pinelliae Rhizoma in different ratios with Wu-tou decoction(2:1,1:1,1:2) by measuring the therapeutic effects in Wu-tou decoction of main seven alkaloids including benzoylaconitine(BA),benzoylmesaconitine(BM),benzoylhypaconitine(BH),hypaconitine(HA),fuziline(FU),niaolin(NE) and deoxyaconitine(DA).The permeability of aconitum alkaloids extract through a Caco-2 cell monolayer was analyzed in the absence and presence of Fritillariae Cirrhosae Bulbus,Fritillariae Thunbergii Bulbus,and Pinelliae Rhizoma,respectively.The results showed that Pinelliae Rhizoma could reduce the absorption of the alkaloids and increase the excretion of the alkaloids,which would attenuate the therapeutic effects of Wu-tou decoction.Therefore.Pinelliae Rhizoma is an incompatible herb of Wu-tou decoction because of the inhibition of the absorption of alkaloids in the intestine.And that Fritillariae Cirrhosae Bulbus and Fritillariae Thunbergii Bulbus showed the effects to improve the permeability of the alkaloids in Wu-tou decoction.These effects of these two herbs were similar,but the former was stronger than the latter,which most likely is clue to the fact that the compositions of these two traditional Chinese medicines are similar.The in vitro data suggests that the compounds such as fritillary presented in alkaloids in the formula maybe improve the therapeutic function caused by the increased bioavailability of alkaloids in intestine. | Hong Kan Wen-Yue Jiang Ru Ding Zhong-Ying Liu Zi-Feng Pi Zhi-Qiang Liu | 2015 | Chinese Chemical Letters2015，26，5： | 5 |
| 13 | Design and test of 4ZZ-4A2 full-hydraulic self-propelled jujube harvester显示文摘The cultivated area of jujube in Xinjiang has increased rapidly in recent years.While the jujube harvest by hand has the shortage of high labor intensity,low efficiency and high labor cost,in addition,the harvesting machinery applying to dwarf and dense planting mode of jujubes is unavailable in Xinjiang.The 4ZZ-4A2 based on the full-hydraulic self-propelled jujube harvester was designed to solve the above problems.The harvester was mainly composed of a frame,a vibrating device,a jujube collecting and conveying device,an air separation device,a steering system,a hydraulic system and a jujube suction device and was capable of completing vibration,collection,conveying,cleaning and sundries removal work of jujubes through one step.The jujubes dropped on the ground were picked up at the same time.The AMESim simulation software was adopted to perform simulation analysis on the overall hydraulic system.The results showed that the speed of the vibrating motor was stable at about 650 r/min(the corresponding vibration frequency is 10.83 Hz)with the torque of 80 N·m,the speed of the conveyor motor was stable at 77 r/min with the torque of 77 N·m;the speed of the fan motor was stable at 54 r/min with the torque of 53.6 N·m;the speed of the walking motor fluctuated around 100 r/min with the torque of about 1000 N·m;the hydraulic steering system responded rapidly and could satisfy the actual working requirements of the jujube harvester.The jujube garden test results showed that the harvester could reach to the optimum harvesting effect when running at the speed of 0.5 m/s.Under such speed,the ground jujube picking rate was 45.1%,the tree jujube harvesting rate was 93.2%,the loss rate was 2.9%,and the damage rate was 0.9%.This study can provide theoretical basis and technical support for the jujube harvester. | Wei Fu Zhiyuan Zhang Kai Ding Weibin Cao Za Kan Junbing Pan Yudong Liu | 2018 | International Journal of Agricultural and Biological Engineering2018，11，4： | 4 |
| 14 | Outcomes of percutaneous coronary intervention in patients ≥ 75 years: one-center study in a Chinese patient group显示文摘ObjectiveTo 调查临床并且病人 ≥ 的 perioperative 特征;75 经历经皮的冠的干预(一种总线标准) 并且从 2011 年 12 月经历了一种总线标准到 2013 年 11 月的连续题目被划分在这特定的病人 group.Methods1,035 评估与短期的一种总线标准以后的死亡有关的风险因素成四个范畴:(1 ) 有稳定的咽峡炎(SA ) 的病人 ≥75 年(n = 58 ) ;(2 ) 有 SA < 的病人;75 年(n = 218 ) ;(3 ) 有急性冠的症候群(交流) 的病人 ≥75 年(n = 155 ) ;(4 ) 有交流 < 的病人;75 年(n = 604 ) 。multivariable 逻辑回归分析被进行在病人 ≥ 检测六月的死亡的风险因素;75 年经历了一种总线标准。临床的 comorbidities,在里面医院生物化学的指示物, perioperative 数据,在里面医院和六月的结果与更年轻的组,一起在四 groups.ResultsCompared 之中被分析并且比较病人 ≥75 年是更可能的有高血压,中风的历史,长期的妨碍的肺的疾病,外部脉管的疾病,心脏性的吃惊和恶意的心律不齐,并且他们与相对更低的重量,血红素,白朊, triglyceride,更高的 creatinine,尿酸,脲氮和 pro-BNP 进入医院。左主要动脉损害,多容器,钙化的损害,长期完全吸藏也是更可能的在老组被看。Univariate 分析揭示了那年龄 ≥在承认,紧急情况一种总线标准,优先的击和长期的肾疾病的 85 年,心脏性的吃惊或严重心律不齐与在老病人 ≥ 的六月的死亡有关;75 年经历了一种总线标准。Multivariable 逻辑回归证明在承认,长期的肾疾病和优先的击的心脏性的吃惊或严重心律不齐是在老病人 ≥ 预言六月的死亡的独立风险因素;75 年经历了 PCI.ConclusionsOur 数据的人,显示出那与在 75 年下面的病人相比,老病人(≥75 年) 经历了一种总线标准的人,有经常伴有 multi-comorbidities, severer 承认条件和复杂冠的损害的死亡,和更多的相对更高的风险。因素和更多强烈地在乎的风险的更好的评估应该被带去病人 ≥75 年经历了一种总线标准治疗减少复杂并发症。 | Peng-Fei CHEN Dan-Ning WANG Kan CHEN Chun LIANG Yu-Sheng RENG Jing YANG Ru DING Jacob Blackwell De-Ning LIAO | 2015 | Journal of Geriatric Cardiology2015，12，6： | 4 |
| 15 | Polluted lake restoration to promote sustainability in the Yangtze River Basin,China显示文摘China has sought to address water pollution in the last decade by introducing a wide range of laws and regulations(Table S1), which led to nationwide water quality improvement ^([1]). However, recent quantitative assessment of progress toward Sustainable Development Goals (SDGs) in China suggests that some SDGs underpinning goals for water pollution and biodiversity have not been met ^([2]). | Boqiang Qin Yunlin Zhang Jianming Deng Guangwei Zhu Jianguo Liu David P.Hamilton Hans W.Paerl Justin D.Brookes Tingfeng Wu Kai Peng Yizhou Yao Kan Ding Xiaoyan Ji | 2022 | National Science Review2022，9，1： | 4 |
| 16 | The molecular chaperone Hsp90α deficiency causes retinal degeneration by disrupting Golgi organization and vesicle transportation in photoreceptors显示文摘Heat shock protein 90(Hsp90)is an abundant molecular chaperone with two isoforms,Hsp90α and Hsp90p.Hsp90β deficiency causes embryonic lethality,whereas Hsp90α deficiency causes few abnormities except male sterility.In this paper,we reported that Hsp90α was exclusively expressed in the retina,testis,and brain.Its deficiency caused retinitis pigmentosa(RP),a disease leading to blindness.In Hsp90α-deficient mice,the retina was deteriorated and the outer segment of photoreceptor was deformed.Immunofluorescence staining and electron microscopic analysis revealed disintegrated Golgi and aberrant intersegmental vesicle transportation in Hsp90α-deficient photoreceptors.Proteomic analysis identified microtubule-associated protein IB(MAP1B)as an Hsp90α-associated protein in photoreceptors.Hspcx deficiency increased degradation of MAP1B by inducing its ubiquitination,causing a-tubulin deacetylation and microtubule destabilization.Furthermore,the treatment of wild-type mice with 17-DMAG,an Hsp90 inhibitor of geldanamycin derivative,induced the same retinal degeneration as Hsp90α deficiency.Taken together,the microtubule destabilization could be the underlying reason for Hsp90α deficiency-induced RP. | Yuan Wu Xiudan Zheng Yubo Ding Min Zhou Zhuang Wei Tao Liu Kan Liao | 2020 | Journal of Molecular Cell Biology2020，12，3： | 4 |
| 17 | Studies on the intestinal absorption of the alkaloids in the Gancaofuzi decoction in a Caco-2 cell culture system by UPLC–MS/MS analysis显示文摘In this study, the Caco-2 cell monolayer model was used to research the characteristic absorption and efflux of five diterpenoid alkaloids in Gancaofuzi decoction. An ultra performance liquid chromatography coupled with tandem mass spectrometry(UPLC–MS/MS) method was developed for the determination of the simulated intestinal transport of five diterpenoid alkaloids with reserpine as internal standard. The use of the apparent permeability coefficient(Papp) and efflux rate(Er) was instituted to evaluate the intestinal absorption of the alkaloids. Transport of the five alkaloids in Caco-2cell monolayer model was observed to better understand whether the intestinal absorption of alkaloids was influenced by the compatibility of four herbs in Gancaofuzi decoction. The results show that the Papp values of the five diterpenoid alkaloids were all more than 1 * 10^(-6)cm/s, confirming that the processes of permeability were valid. The flux of the alkaloids was time-dependent, and the intestinal absorption mechanism of the five alkaloids was mainly based on passive transport. The compatibility of Heishunpian, Baizhu, Guizhi and Gancao can reduce the intestinal absorption of alkaloids, especially the most toxic hypaconitine, and the attenuated effect of mixed herbal water extracts was better than that of different herbs' water extracts combination. The results prove that compatibility of four herbs in Gancaofuzi decoction is rational. | Ru Ding Yong Jian Hong Kan Zhong-Ying Liu Shu Liu Zhi-Qiang Liu Jun Zhou | 2016 | Chinese Chemical Letters2016，27，6： | 3 |
| 18 | Joint elastic-electrical properties of sediments in the Yellow Sea显示文摘We measured in the laboratory compressional wave velocity and electrical resistivity on 434 sediment samples collected from the Yellow Sea to study the joint elastic-electrical properties of marine sediments. Porosity was found to reduce both elastic velocity and electrical resistivity of the marine sediments in a non-linear fashion; velocity showed an approximate linear increase with increasing logarithm of resistivity. Various effective medium models either implemented or developed were compared with the new dataset. The model results showed that the combined self-consistent approximation and differential effective medium model using critical porosity of 0.6 and 0.5 for velocity and resistivity respectively gave a reasonable description of the joint elastic-electrical behaviors of the marine sediments. The joint elastic-electrical properties of the marine sediments established would be used to estimate resistivity from measured velocity and vice versa, and could also be suitable for detection of gas hydrate or other suitable targets from joint seismic-resistivity surveys. | HAN TongCheng LIU BaoHua KAN GuangMing MENG XiangMei DING ZhongJun | 2012 | Science China Earth Sciences2012，55，1： | 3 |
| 19 | Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse显示文摘在 adipocytes 的类脂化合物微滴用作动物的主要长期的精力存储仓库。正在增加类脂化合物微滴不仅仅是一个静态的中立类脂化合物存储地点,而是事实上有活力、多功能的细胞器的识别。在结构上,类脂化合物微滴由嵌入在或跳了到 phospholipid 层的 phospholipid 单层和蛋白质包围的一个中立类脂化合物核心组成。类脂化合物微滴的表面上的蛋白质对微滴结构和动力学关键。理解类脂化合物有大中央类脂化合物微滴的主要 adipocyte 的联系微滴的 proteome,从 C57BL/6 老鼠的白脂肪质的织物的类脂化合物微滴被孤立。,和由结合的液体层析双人脚踏车团 spectrometry。以前包括 73 的 193 蛋白质的一个总数 unreported 蛋白质被识别。而且,编码同位素的亲密关系标签(ICAT ) 被用来比较类脂化合物的差别在瘦的正常和高脂肪的导致食谱的肥胖的 C57BL/6 老鼠之间的联系微滴的 proteomes。23,蛋白质由 ICAT 分析确定了, 3 蛋白质是起来调整的, 4 蛋白质在从肥胖的老鼠的脂肪质的织物的类脂化合物微滴是下面调整的。重要地,类脂化合物微滴, perilipin A 和 vimentin 的二结构的蛋白质,极大地从肥胖的老鼠在脂肪质的织物的类脂化合物微滴被减少,为类脂化合物的含有的减少的蛋白质机械微滴稳定性。 | Yubo Ding Yibo Wu Rong Zeng Kan Liao | 2012 | Acta Biochimica et Biophysica Sinica2012，44，5： | 3 |
| 20 | The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability显示文摘Hsp90 is an abundant and special molecular chaperone considered to be the regulator of many transcription factors and signaling kinases. Its high abundance is indicative of its involvement in some more fundamental processes. In this study, we provide evidence that Hsp90 is required for microtubule stabilization, Golgi organization, and vesicular trafficking. We showed that Hsp90 is bound to microtubule-associated protein 4 (MAP4), which is essential for maintaining microtubule acetylation and stabilization. Hsp90 depletion led to the decrease in MAP4, causing microtubule deacetylation and destabilization. Furthermore, in Hsp90-depleted cells, the Golgi apparatus was fragmented and anterograde vesicle trafficking was impaired, with phenotypes similar to those induced by silencing MAP4. These disruptive effects of Hsp90 depletion could be rescued by the expression of exogenous MAP4 or the treatment of trichostatin A that increases microtubule acetylation as well as stability. Thus, microtubule stability is an essential cellular event regulated by Hsp90. | Yuan Wu Yubo Ding Xiudan Zheng Kan Liao | 2020 | Journal of Molecular Cell Biology2020，12，6： | 3 |
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