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您的检索式：作者名="Kashan Memon"
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| 1 | A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes显示文摘Investigation of oocyte membrane permeability plays a crucial role in fertility preservation,reproductive medicine,and reproductive pharmacology.However,the commonly used methods have disadvantages such as high time consumption,low efficiency,and cumbersome data processing.In addition,the developmental potential of oocytes after measurement has not been fully validated in previous studies.Moreover,oocytes can only maintain their best status in vitro within a very limited time.To address these limitations,we developed a novel multichannel microfluidic chip with newly designed micropillars that provide feasible and repeatable oocyte capture.The osmotic responses of three oocytes at different or the same cryoprotectant(CPA)concentrations were measured simultaneously,which greatly improved the measurement efficiency.Importantly,the CPA concentration dependence of mouse oocyte membrane permeability was found.Moreover,a neural network algorithm was employed to improve the efficiency and accuracy of data processing.Furthermore,analysis of fertilization and embryo transfer after perfusion indicated that the microfluidic approach does not damage the developmental potential of oocytes.In brief,we report a new method based on a multichannel microfluidic chip that enables synchronous and nondestructive measurement of the permeability of multiple oocytes. | Zhongrong Chen Kashan Memon Yunxia Cao Gang Zhao | 2020 | Microsystems & Nanoengineering2020，6，1： | 2 |
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