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| 1 | Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production显示文摘AIM: To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes. METHODS: Cytokine production in human peripheral blood mononuclear cells (PBMC) in response to stimulation with eleven different potentially probiotic bacterial strains from Streptococcus , Lactobacillus , Bifidobacterium , Lactococcus , Leuconostoc and Propionibacterium genera was analysed. Production and mRNA expression of TNF-α, IL-12, IFN-γ and IL-10 were determined by ELISA and Northern blotting, respectively. RESULTS: All tested bacteria induced TNF-α production. The best inducers of Th1 type cytokines IL-12 and IFN-γ were Streptococcus and Leuconostoc strains. All Bifidobacterium and Propionibacterium strains induced higher IL-10 production than other studied bacteria. Stimulation of PBMC with any bacterial combinations did not result in enhanced cytokine production suggesting that different bacteria whether gram-positive or gram- negative compete with each other during host cell interactions.CONCLUSION: The probiotic S. thermophilus and Leuconostoc strains are more potent inducers of Th1 type cytokines IL-12 and IFN-γ than the probiotic Lactobacillus strains. Bacterial combinations did not result in enhanced cytokine production. | Riina A Kekkonen Elina Kajasto Minja Miettinen Ville Veckman Riitta Korpela Ilkka Julkunen | 2008 | World Journal of Gastroenterology2008，14，8： | 9 |
| 2 | Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome显示文摘 | Mirjana Rajili?–Stojanovi? Elena Biagi Hans G.H.J. Heilig Kajsa Kajander Riina A. Kekkonen Sebastian Tims Willem M. de Vos | 2011 | Gastroenterology2011，，5： | 8 |
| 3 | Potentially probiotic bacteria induce efficient maturation but differential cytokine production in human monocyte-derived dendritic cells显示文摘AIM: To analyze the ability of nine different potentially probiotic bacteria to induce maturation and cytokine production in human monocyte-derived dendritic cells (moDCs). METHODS: Cytokine production and maturation of moDCs in response to bacterial stimulation was analyzed with enzyme-linked immunosorbent assay (ELISA) and flow cytometric analysis (FACS), respectively. The kinetics of mRNA expression of cytokine genes was determined by Northern blotting. The involvement of different signaling pathways in cytokine gene expression was studied using specific pharmacological signaling inhibitors. RESULTS: All studied bacteria induced the maturation of moDCs in a dose-dependent manner. More detailed analysis with S. thermophilus THS, B. breve Bb99, and L. lactis subsp. cremoris ARH74 indicated that these bacteria induced the expression of moDC maturationmarkers HLA class Ⅱ and CD86 as efficiently as pathogenic bacteria. However, these bacteria differed in their ability to induce moDC cytokine gene expression. S. thermophilus induced the expression of pro-inflammatory (TNF-α, IL-12, IL-6, and CCL20) and Th1 type (IL-12 and IFN-γ) cytokines, while B. breve and L. lactis were also potent inducers of anti- inflammatory IL-10. Mitogen-activated protein kinase (MAPK) p38, phosphatidylinositol 3 (PI3) kinase, and nuclear factor-kappa B (NF-κB) signaling pathways were shown to be involved in bacteria-induced cytokine production. CONCLUSION: Our results indicate that potentially probiotic bacteria are able to induce moDC maturation, but their ability to induce cytokine gene expression varies significantly from one bacterial strain to another. | Sinikka Latvala Taija E Pietil Ville Veckman Riina A Kekkonen Soile Tynkkynen Riitta Korpela Ilkka Julkunen | 2008 | World Journal of Gastroenterology2008，14，36： | 8 |
| 4 | Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults显示文摘AIM: To evaluate the effects of three potentially anti- inflammatory probiotic bacteria from three different genera on immune variables in healthy adults in a clinical setting based on previous in vitro characterization of cytokine responses. METHODS: A total of 62 volunteers participated in this randomized, double-blind and placebo-controlled parallel group intervention study. The volunteers were randomized to receive a milk-based drink containing either Lactobacillus rhamnosus GG (LGG), Bifidobacterium animalis ssp. lactis Bb12 (Bb12), or Propionibacterium freudenreichii ssp. shermanii JS (PJS) or a placebo drink for 3 wk. Venous blood and saliva samples were taken at baseline and on d 1, 7 and 21. Fecal samples were collected at baseline and at the end of intervention. RESULTS: The serum hsCRP expressed as the median AUC0-21 (minus baseline) was 0.018 mg/L in the placebo group, -0.240 mg/L in the LGG group, 0.090 mg/L in the Bb12 group and -0.085 mg/L in the PJS group (P = 0.014). In vitro production of TNF-α from in vitro cultured peripheral blood mononuclear cells (PBMC) was significantly lower in subjects receiving LGG vs placebo. IL-2 production from PBMC in the Bb12 group was significantly lower compared with the other groups. CONCLUSION: In conclusion, probiotic bacteria have strain-specific anti-inflammatory effects in healthy adults. | Riina A Kekkonen Netta Lummela Heli Karjalainen Sinikka Latvala Soile Tynkkynen Salme Jrvenp Hannu Kautiainen Ilkka Julkunen Heikki Vapaatalo Riitta Korpela | 2008 | World Journal of Gastroenterology2008，14，13： | 6 |
| 5 | Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells显示文摘AIM:To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS:Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L.rhamnosus) GG (LGG),L.rhamnosus Lc705 (Lc705),Propionibacterium freudenreichii ssp.shermanii JS (PJS) and Bifidobacterium animalis ssp.lactis Bb12 (Bb12) and their combination for 3 or 24 h,and were subjected to global microarray analysis using an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS:LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G,respectively) and histamine H4 receptor.LGG,Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation,and on several genes that encoded proteins with a pro-inflammatory impact,such as interleukin (IL)-8 and tumour necrosis factor alpha.Also genes that encoded proteins with anti-inflammatory functions,such as IL-10,were upregulated.CONCLUSION:Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes,and by inducing a pro-inflammatory response. | Anna Oksaharju Matti Kankainen Riina A Kekkonen Ken A Lindstedtt Petri T Kovanen Riitta Korpela Minja Miettinen | 2011 | World Journal of Gastroenterology2011，17，6： | 3 |
| 6 | The impacts of EU CO2 emission trading on electricity markets and electricity consumers in Finland 显示文摘 | Kara M Syri S Lehtila A Helynen S Kekkonen V Ruska M Forsstrom J | 2006 | Energy Economics2006，2，30： | 1 |
| 7 | Formation, adsorption and interactions of poly - dadmac/pectic acid complexes 显示文摘 | Kekkonen J Lattu H Stenius P | 2002 | Journal of Pulp and Paper Science2002，28，1： | 1 |
| 8 | Pharmacoki-netics of mifepristone after low oral doses显示文摘 | Kekkonen R Heikinheimo O Mandelin E | 1996 | Contraception1996，54，4： | 1 |
| 9 | The formation of deposits on polymer surface in paper machine wet end显示文摘 | Kallio T Kekkonen J Stenius P | 2004 | J Adhesion2004，80，： | 1 |
| 10 | Nurse Empower- ment, Job--related Satisfaction, and Organizational Com- rnitment 显示文摘 | Kekkonen Leino--Kilpi H Katajisto J | 2003 | Nurse Care Qual2003，18，3： | 1 |
| 11 | Effect of mineralogy and reducing agent on reduction of saprolitic nickel ores显示文摘 | BUNJAKU A KEKKONEN M PIETILA K TASKINEN P | 2012 | Mineral Processing and Extractive Metallurgy2012，121，3： | 1 |
| 12 | Lactobacillus rhamnosus GG and Streptococcus thermophilus induce suppressor of cytokine signalling 3 (SOCS3) gene expression directly and indirectly via interleukin-10 in human primary macrophagse显示文摘 | Latvala S Miettinen M Kekkonen RA | | 0，，01： | 1 |
| 13 | Alcohol use among adolescents,aggressive behaviour,and internalizing problems显示文摘 | Kivimaki P Kekkonen V Valtonen H | 2014 | Journal of adolescence2014，37，6： | 1 |
| 14 | Fouling in the paper machine wet end显示文摘 | Timo Kallio Juha Kekkonen | 2005 | TAPPI Journal2005，4，10： | 1 |
| 15 | Formation,adsorption and interactions of poly-dadmac/pectic acid complexes显示文摘 | Lattu H Stenius P | 2002 | Journal of Pulp and Paper Science2002，28，1： | 1 |
| 16 | The impacts of EU CO 2 emissions trading on electricity markets and electricity consumers in Finland显示文摘 | M. Kara S. Syri A. Lehtil? S. Helynen V. Kekkonen M. Ruska J. Forsstr?m | 2006 | Energy Economics2006，，2： | 1 |
| 17 | The pharmacokinetics of mifepristonc in humans reveal insights into differential mechanisms of antiprogestin action显示文摘 | Heikinheimo O Kekkonen R Lahteenmaki P | 2005 | Contraception2005，68，6： | 1 |
| 18 | Immunomodulatory Effects of Probiotic 显示文摘 | KEKKONEN R A | 2009 | Aus- tralian Journal of Dairy Technology2009，64，： | 1 |
| 19 | Lactobacillus rhamno-sus GG and Streptococcus thermophilus induce suppressor of cyto-kine signalling 3 ( SOCS3 ) gene expression directly and indirectlyvia interleukin-10 in human primary macrophages 显示文摘 | Latvala S Miettinen M Kekkonen RA | 2011 | Clin ExpImmunol2011，165，1： | 1 |
| 20 | DNA barcode-based delineation of putative species:efficient start for taxonomic workflows显示文摘 | Kekkonen M Hebert PD | 2014 | Mol Ecol Resour2014，14，4： | 1 |
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