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| 1 | Microwave activation of enzymatic catalysis 显示文摘 | Young D D Nichols J Kelly R M | 2008 | Journal of the American ChemicalSociety2008，130，10： | 1 |
| 2 | Microwave aetiva tion of enzymatic catalysis显示文摘 | YOUNG D D NICHOLS J KELLY R M | 2008 | Journal of the American Chemical Society2008，130，10： | 1 |
| 3 | Patient-Reported Symptoms in Dry Dye Disease显示文摘 | Kelly K. Nichols | 2006 | The Ocular Surface2006，，3： | 1 |
| 4 | Growth and physiological response of six Australian rainforest tree species to a light gradient 显示文摘 | KELLY J JOSE S NICHOLS J D | 2009 | Forest Ecology and Management2009，257，1： | 1 |
| 5 | Performance and Repeatability of the NEI-VFQ-25 in Patients With Dry Eye显示文摘 | Kelly K. Nichols G. Lynn Mitchell Karla Zadnik | 2002 | Cornea2002，，6： | 1 |
| 6 | The Repeatability of Clinical Measurements of Dry Eye显示文摘 | Kelly K. Nichols G. Lynn Mitchell Karla Zadnik | 2004 | Cornea2004，，3： | 1 |
| 7 | The lack of association between signs and symptoms in patients with dry eye disease显示文摘 | Kelly K Nichols J Jason J | 2004 | Cornea2004，23，8： | 1 |
| 8 | Microwave activation of enzymatic catalysis显示文摘 | Yang Douglas D Nichols Jason Kelly Robert M | 2008 | J Am Chern Soc2008，130，10： | 1 |
| 9 | Impact of post-ruminally infused macronutrients on bovine mammary gland expression of genes involved in fatty acid synthesis, energy metabolism, and protein synthesis measured in RNA isolated from milk fat显示文摘Background: Characterising the regulation of milk component synthesis in response to macronutrient supply is critical for understanding the implications of nutritional interventions on milk production. Gene expression in mammary gland secretory cells was measured using RNA isolated from milk fat globules from 6 Holstein-Friesian cows receiving 5-d abomasal infusions of saline, essential amino acids(AA), or glucose(GG) or palm olein(LG)without(LAA) or with(HAA) essential AA, according to a 6 × 6 Latin square design. RNA was isolated from milk fat samples collected on d 5 of infusion and subjected to real-time quantitative PCR. We hypothesised that m RNA expression of genes involved in de novo milk fatty acid(FA) synthesis would be differently affected by GG and LG,and that expression of genes regulating transfer of tricarboxylic acid cycle intermediates would increase at the HAA level. We also hypothesised that the HAA level would affect genes regulating endoplasmic reticulum(ER)homeostasis but would not affect genes related to the mechanistic target of rapamycin complex 1(m TORC1) or the integrated stress response(ISR) network.Results: Infusion of GG did not affect de novo milk FA yield but decreased expression of FA synthase(FASN).Infusion of LG decreased de novo FA yield and tended to decrease expression of acetyl-Co A carboxylase 1(ACC1).The HAA level increased both de novo FA yield and expression of ACC1, and tended to decrease expression of mitochondrial phosphoenolpyruvate carboxykinase(PCK2). m RNA expression of m TORC1 signaling participants was not affected by GG, LG, or AA level. Expression of the ε subunit of the ISR constituent eukaryotic translation initiation factor 2 B(EIF2 B5) tended to increase at the HAA level, but only in the presence of LG. X-box binding protein 1(XBP1) m RNA was activated in response to LG and the HAA level.Conclusions: Results show that expression of genes involved in de novo FA synthesis responded to glucogenic,lipogenic, and aminogenic substrates, whereas genes regulating intermediate flux through the tricarboxylic acid cycle were not majorly affected. Results also suggest that after 5 d of AA supplementation, milk protein synthesis is supported by enhanced ER biogenesis instead of signaling through the mTORC1 or ISR networks. | Kelly Nichols AndréBannink Jurgen van Baal Jan Dijkstra | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 0 |
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