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| 1 | Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood,bone marrow and peritoneal lavage of pancreatic carcinoma patients显示文摘AIM: To evaluate the diagnostic potential of cytokeratin-19 (CK-19) mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS: Sixty-eight patients with pancreatic cancer (n = 37), chronic pancreatitis (n = 16), and non-pan- creatic benign surgical diseases (n = 15, control group) were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS: CK-19 mRNA expression was increased in 24 (64%) blood samples and 11 (30%) of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 (40%) of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors (G2/G3). Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancerpatients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION: Disseminated tumor cells can be detected in patients with pancreatic ductal adenocar- cinoma by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers. | Katrin Hoffmann Christiane Kerner Wolfgang Wilfert Marc Mueller Joachim Thiery Johann Hauss Helmut Witzigmann | 2007 | World Journal of Gastroenterology2007，13，2： | 20 |
| 2 | The cellular and molecular basis of hyperthermia显示文摘 | Bert Hildebrandt Peter Wust Olaf Ahlers Annette Dieing Geetha Sreenivasa Thoralf Kerner Roland Felix Hanno Riess | 2002 | Critical Reviews in Oncology and Hematology2002，，： | 3 |
| 3 | Late stent thrombosis after implantation of a sirolimus-eluting stent显示文摘 | Kerner A Gruberg L Kapeliovich M | 2003 | Catheter Cardiovasc Interv2003，60，4： | 1 |
| 4 | Characterization of Environmental Estrogens in River Water Using a Three Pronged Approach:Ac- tive and Passive Water Sampling and the Analysis of Accumulated Estrogens in the bile of Caged Fish 显示文摘 | Vermeirssen E L Kerner O Schenenberger R | 2005 | Environ Sci Technol2005，39，21： | 1 |
| 5 | A novel standardized algorithm for evaluating patients with painful total knee arthroplasty using combined single photon emission tomography and conventional computerized tomography显示文摘 | Michael T. Hirschmann Farhad Iranpour Praveen Konala Anna Kerner Helmut Rasch Justin P. Cobb Niklaus F. Friederich | 2010 | Knee Surgery Sports Traumatology Arthroscopy2010，，7： | 1 |
| 6 | Three phrase traffic theory and high- way capacity显示文摘 | KERNER B S | 2004 | Physical A: Statistical Mechanics and its Applications2004，333，： | 1 |
| 7 | Early and late of plasma DNA assay for risk assessment in blunt multiple trauma显示文摘 | Spielmann S Kerner T Ahlers O | 2003 | Clin Chem2003，49，8： | 1 |
| 8 | Troponin Positibe, CK - MB negative acute myocardial infarction: clinical, electrocardiographic and angiographic characteristics 显示文摘 | Gruberg L Sudarsky D Kerner A | 2005 | J Invasive Cardiol2005，20，3： | 1 |
| 9 | Tunneling, temperature, and Taub-NUT black holes显示文摘 | Ryan Kerner Mann R B | 2006 | Phys Rev D2006，73，10： | 1 |
| 10 | Immediate ambula- tion after embryo transfer: a prospective study 显示文摘 | Bar - Hava I Kerner R Yneli R | 2005 | Fertil Steri12005，83，3： | 1 |
| 11 | Local cluster effect in different traffic flow models显示文摘 | Herrmann M Kerner B S | 1998 | Physica A1998，，255： | 1 |
| 12 | The elastic and thermo-elastic properties of composite media 显示文摘 | KERNER E H | 1956 | Proc Phys Soc B1956，69，： | 1 |
| 13 | Experimental features of the emer- gence of moving jams in free traffic flow显示文摘 | KERNER B S | 2000 | Journal of Physics A: Mathematical and General2000，33，26： | 1 |
| 14 | Early detection of increased tumour necrosis factor alpha (TNFalpha) and soluble TNF receptor protein plasma levels after trauma reveals associations with the clinical course显示文摘 | Spielmann S Kerner T Ahlers O | | 0，，： | 1 |
| 15 | Accurate and efficient seismic modeling in random media 显示文摘 | KNEIB G KERNER C | 1993 | Geophysics1993，58，4： | 1 |
| 16 | Early detection of in- creased tumor necrosis factor alpha(TNFalpha) and soluble TNF receptor protein plasma levels after trauma reveals associations with the clinical course 显示文摘 | Spielmann S Kerner T Ahlers O | 2001 | Acta Anaesthesiol Scand2001，45，3： | 1 |
| 17 | Experimental Properties of Complexity in Traffic Flow显示文摘 | KERNER B S REHBORN H | 1996 | Physical Review E1996，53，5： | 1 |
| 18 | Early detection of increased tumour necrosis factor alpha(TNF alpha)and soluble TNF receptor protein plasma levels after trauma reveals associations with the clini-cal course显示文摘 | Spielmann S Kerner T Ahlers O | 2001 | Acta Anaesthesiol scand2001，45，3： | 1 |
| 19 | Cluster effect in initially homogeneous traffic flow 显示文摘 | Kerner B S Konhuser P | 1993 | Phy Rev E1993，48，： | 1 |
| 20 | Three phase Traffic Theory and Highway Capacity显示文摘 | KERNER B S | 2004 | Physica A : Statistical Mechanics and Its Applications2004，333，： | 1 |
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