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| 1 | Construction of DNA Fingerprint Using SSR Marker for Hybrid Rice Cultivars Approved by Hunan Province显示文摘[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved by Hunan Province. [Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the obtained data could identify different hybrid rice cultivars. [Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification. | Qingcai ZHAN Zifa XIAO Keyong ZHU Zhixi LIU Zhaohua LI Shuzhen ZENG | 2012 | Agricultural Biotechnology2012，1，4： | 1 |
| 2 | 以正丁胺为扩链剂的水性聚氨酯酡合成及表征显示文摘以异氟二酮二异氰酸酯(IPDI)、聚四氢呋喃醚(PTMG)、二羟甲基丙酸(DMPA)为单体,用正丁胺、乙二醇、乙二胺为扩链剂合成不同类的聚氨酯皮革涂饰剂。干燥成膜后用FTIR、DSC和吸水率表征其化学结构和性能。结果表明,用正丁胺为扩链剂的聚氨酯膜的性能比其他两种扩链剂的性能要好。断裂伸长率最高可以达到14.54%;玻璃化转变温度(Tg)能达到-82℃;吸水率也相对较低。 | Keyong Tang Bing Zhu Xuejng Zheng Dennis C.Shelly 王梓丞(译) | 2010 | 北京皮革(中外鞋讯)(下)2010，，8： | 0 |
| 3 | Semantics Analytics of Origin-Destination Flows from Crowd Sensed Big Data显示文摘Monitoring,understanding and predicting Origin-destination(OD)flows in a city is an important problem for city planning and human activity.Taxi-GPS traces,acted as one kind of typical crowd sensed data,it can be used to mine the semantics of OD flows.In this paper,we firstly construct and analyze a complex network of OD flows based on large-scale GPS taxi traces of a city in China.The spatiotemporal analysis for the OD flows complex network showed that there were distinctive patterns in OD flows.Then based on a novel complex network model,a semantics mining method of OD flows is proposed through compounding Points of Interests(POI)network and public transport network to the OD flows network.The propose method would offer a novel way to predict the location characteristic and future traffic conditions accurately. | Ning Cao Shengfang Li Keyong Shen Sheng Bin Gengxin Sun Dongjie Zhu Xiuli Han Guangsheng Cao Abraham Campbell | 2019 | Computers, Materials & Continua2019，，7： | 0 |
| 4 | Effect of Pb, Hg and Cd in soil on scavenging system of activated oxygen in tobacco leaves显示文摘Pb, Hg and Cd will affect human health by vegetative chain. In recent years, people more and more are concerned about heavy metal pollution of the soil-plant system. Many researchers studied the effect of single heavy metal on growth, photosynthesis, respiration and quality of plants, but few systematicstudies of the effect of heavy metals on the scavenging system of activated oxygen of plants. This note systematically studies the effect of Pb, Hg and Cd in soil on the scavenging system of activated oxygen in tobacco leaves. 1 Material and methods Material: Tobacco (Nicoticana tabacum L). Methods: Cultivating and measuring methods were according to reference. 2 Results Results of this study indicate that the common effect of Pb, Hg and Cd on activity of CAT is obvi- | YAN Chongling~(1,2), HONG Yetang~1, WANG Shijie~1, LIN Peng~3, YANG Xianke~2, FU Shunzhen~2, ZHU Keyong~2 and WU Shanqi~2 1. State Key Laboratory of Environmental Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China 2. Guizhou Institute of Environmental Protection, Guiyang 550002, China 3. Department of Biology, Xiamen University, Xiamen 361005, China | 1999 | Chinese Science Bulletin1999，44，S2： | 0 |
| 5 | Characteristics of rare earth elements in soils in Guizhou carbonate region显示文摘IN recent years, rare earth element (REE) being used in agriculture has gained greater progress and madeus reach some common views. The centent of REE (especially soluble REE) in soils has significantcorrelation with that of REE plant absorbed from soil. Therefore, it is very necessary to clarify contentvariation and background values of REE (especially soluble REE ) from the angles of relation of supplyand demand between soil and plants, for reasonably using REE and formulating relevant health standardin the region, avoiding excessive using REE and taking up soil environmental capacity of REE to result insoil or water-body polluted and sometime for researching rock weathering, soil-forming process and soilclassification in the region. This note deals with characteristics of content and distribution of REE andsoluble REE in soils of Guizhou carbonate region. | YAN Chongling~(1,2), HONG Yetang~1, WANG Shijie~1, FU Shunzhen~2, ZHU Keyong~2 and WU Shanqi~2 1. State Key Laboratory of Environmental Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China 2. Guizhou Institute of Environmental Protection, Guiyang 550002, China | 1999 | Chinese Science Bulletin1999，44，S2： | 0 |
| 6 | Translocation of telomerase reverse transcriptase coincided with ATP release in postnatal cochlear supporting cells显示文摘The spontaneous bursts of electrical activity in the developing auditory system are derived from the periodic release of adenosine triphosphate(ATP)by supporting cells in the Kölliker’s organ.However,the mechanisms responsible for initiating spontaneous ATP release have not been determined.Our previous study revealed that telomerase reverse transcriptase(TERT)is expressed in the basilar membrane during the first postnatal week.Its role in cochlear development remains unclear.In this study,we investigated the expression and role of TERT in postnatal cochlea supporting cells.Our results revealed that in postnatal cochlear Kölliker’s organ supporting cells,TERT shifts from the nucleus into the cytoplasm over time.We found that the TERT translocation tendency in postnatal cochlear supporting cells in vitro coincided with that observed in vivo.Further analysis showed that TERT in the cytoplasm was mainly located in mitochondria in the absence of oxidative stress or apoptosis,suggesting that TERT in mitochondria plays roles other than antioxidant or anti-apoptotic functions.We observed increased ATP synthesis,release and activation of purine signaling systems in supporting cells during the first 10 postnatal days.The phenomenon that TERT translocation coincided with changes in ATP synthesis,release and activation of the purine signaling system in postnatal cochlear supporting cells suggested that TERT may be involved in regulating ATP release and activation of the purine signaling system.Our study provides a new research direction for exploring the spontaneous electrical activity of the cochlea during the early postnatal period. | Yukai Zhang Keyong Tian Wei Wei Wenjuan Mi Fei Lu Zhenzhen Liu Qingwen Zhu Xinyu Zhang Panling Geng Jianhua Qiu Yongli Song Dingjun Zha | 2024 | Neural Regeneration Research2024，19，5： | 0 |
| 7 | Extraction Process and Antioxidant Activity of Polysaccharides from Cyathula Capitata( Wall. ) Moq.显示文摘[Objectives]To optimize the ultrasonic-assisted extraction process of polysaccharides from Cyathula Capitata( Wall.) Moq. and evaluate its antioxidant capacity in vitro. [Methods] The single factor orthogonal design was applied to explore the effects of ultrasonic time,solid-to-liquid ratio,extraction time and ethanol precipitation concentration on the extraction rate of crude polysaccharides from C. Capitata,to obtain the optimal extraction process; DPPH and ABTS^+ free radical scavenging ability was detected to evaluate its in vitro antioxidant activity.[Results]The optimal extraction process of C. Capitata crude polysaccharide was as follows: solid-to-liquid ratio was 40∶ 1,the extraction temperature was 50℃,the ultrasonic time was 80 min. Under such conditions,the extraction rate of C. Capitata crude polysaccharide was 10. 51%,and the purity was 56. 9%; C. Capitata crude polysaccharide has good scavenging ability to DPPH and ABTS^+ free radicals,and its IC_(50) values are 0. 468 and 0. 231 mg/mL,respectively. [Conclusions]The optimized process is simple and feasible,and the C. Capitata polysaccharide has good antioxidant activity in vitro. This experiment provides a basis for the further development and utilization of the C. Capitata polysaccharide. | Jingxia WANG Keyong ZHU Yuanhong SHANG Yuan LIU | 2018 | Medicinal Plant2018，9，5： | 0 |
| 8 | Characteristics,classification and KNN-based evaluation of paleokarst carbonate reservoirs:A case study of Feixianguan Formation in northeastern Sichuan Basin,China显示文摘The Feixianguan Formation reservoirs in northeastern Sichuan are mainly a suite of carbonate platform deposits.The reservoir types are diverse with high heterogeneity and complex genetic mechanisms.Pores,vugs and fractures of different genetic mechanisms and scales are often developed in association,and it is difficult to classify reservoir types merely based on static data such as outcrop observation,and cores and logging data.In the study,the reservoirs in the Feixianguan Formation are grouped into five types by combining dynamic and static data,that is,karst breccia-residual vuggy type,solution-enhanced vuggy type,fractured-vuggy type,fractured type and matrix type(non-reservoir).Based on conventional logging data,core data and formation microscanner image(FMI)data of the Qilibei block,northeastern Sichuan Basin,the reservoirs are classified in accordance with fracture-vug matching relationship.Based on the principle of cluster analysis,K-Nearest Neighbor(KNN)classification templates are established,and the applicability of the model is verified by using the reservoir data from wells uninvolved in modeling.Following the analysis of the results of reservoir type discrimination and the production of corresponding reservoir intervals,the contributions of various reservoir types to production are evaluated and the reliability of reservoir type classification is verified.The results show that the solution-enhanced vuggy type is of high-quality sweet spot reservoir in the study area with good physical property and high gas production,followed by the fractured-vuggy type,and the fractured and karst breccia-residual vuggy types are the least promising. | Yang Ren Wei Wei Peng Zhu Xiuming Zhang Keyong Chen Yisheng Liu | 2023 | Energy Geoscience2023，4，3： | 0 |
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