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| 1 | Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation显示文摘AIM:To investigate large tumor suppressor 1 (LATS1 ) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).METHODS:RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis. RESULTS:Decreased LATS1 expression was found in 127/142 (89.4%) CRC cases with the average ratio of the LATS1 level 10.33 ± 32.64 in CRC patients vs 32.85 ± 33.56 in healthy controls. The lowest expression was found in Dukes' B stage tumors and G1 (welldifferentiated) cells. Hypermethylation of the LATS1 promoter was present in 25/44 (57%) CRC cases analyzed. LATS1 promoter hypermethylation was strongly associated with decreased gene expression; methylated cases showed 162× lower expression of LATS1 than unmethylated cases. Although high-grade MSI (mutation in all three markers) was found in 14/84 (17%) cases and low-grade MSI (mutation in 1-2 markers) was found in 30/84 (36%) cases, we found no association with LATS1 expression. CONCLUSION:Decreased expression of LATS1 in CRC was associated with promoter hypermethylation, but not MSI status. Such reduced expression may promote progression of CRC. | Piotr M Wierzbicki Krystian Adrych Dorota Kartanowicz Marcin Stanislawowski Anna Kowalczyk Janusz Godlewski Iwona Skwierz-Bogdanska Krzysztof Celinski Tomasz Gach Jan Kulig Bartlomiej Korybalski Zbigniew Kmiec | 2013 | World Journal of Gastroenterology2013，19，27： | 8 |
| 2 | Evaluation of enterochromaffin cells and melatonin secretion exponents in ulcerative colitis显示文摘AIM: To study an assessment of the number of enterochromaffin cells and expression of hydroxyindole-Omethyltransferase in colonic mucosa and urine excretion of 6-sulfatoxymelatonin in patients with ulcerative colitis. METHODS: The study included 30 healthy subjects (groupⅠ-C), 30 patients with ulcerative proctitis [group Ⅱ-ulcerative proctitis (UP)] and 30 patients with ulcerative colitis [group Ⅲ-ulcerative colitis (UC)] in acute phases of these diseases. The number of enterochromaffin cells (EC) was estimated in rectal and colonic mucosa. Bioptates were assembled from many different parts of the large intestine. Immunorective cells collected from various parts of the colon were counted according to the Eurovision DAKO (Dako A/S, Copenhagen, Denmark) System in the range of 10 fields in each bioptate at × 200 magnification. The level of mRNA expression of hydroxyindole-O-methyltransferase (HIOMT) in colonic mucosa was estimated with RT-PCR. Urine 6-sulfatoxymelatonin (6-HMS) excretion was determined immunoenzymatically using an IBL (IBL International GmbH, Hamburg, Germany) kit (RE 54031). RESULTS: The number of EC cells in healthy subjects (C) was 132.40 ± 31.26. In patients of group Ⅱ (UP) and group Ⅲ (UC) the number of these cells was higher 225.40 ± 37.35 (P < 0.001) and 225.24 ± 40.50 (P < 0.001) respectively. Similar differences were related to HIOMT expression, which was 1.04 ± 0.36 in group C, 1.56 ± 0.56 (P < 0.01) in group UP and 2.00 ± 0.35 (P < 0.001) in group UC. Twenty-four hour 6-HMS urinary excretion was as follows: C 16.32 ± 4.95 μg/24 h, UP 26.30 ± 7.29 μg/24 h (P < 0.01), UC 42.30 ± 12.56 μg/24h (P < 0.001). A correlation between number of EC cells and 6-HMS excretion was noted in all groups: r = 0.766 in patients with UP, r = 0.703 with UC and r = 0.8551 in the control group; the correlation between the results is statistically significant. CONCLUSION: In the acute phases of both UP and UC, proliferation of EC cells and high expression of HIOMT and urine excretion of 6-HMS is noted. These changes may represent a beneficial response in the anti-inflammatory and defense mechanism. | Cezary Chojnacki Maria Wisniewska-Jarosińska Grazyna Kulig Ireneusz Majsterek Russel J Reiter Jan Chojnacki | 2013 | World Journal of Gastroenterology2013，19，23： | 5 |
| 3 | T-regulatory lymphocytes in peripheral blood of gastric and colorectal cancer patients显示文摘AIM: To assess the absolute number of T-regulatory cells (Tregs; CD4+CD25+Foxp3+) in the peripheral blood of gastric and colorectal cancer patients. METHODS: We enrolled 70 cancer patients (33 gastric cancer, 37 colorectal cancer) and 17 healthy volunteers. The CD3+CD4+ lymphocytes and CD4+CD25+Foxp3+ Tregs in the peripheral blood were analyzed with flow cytometry. The absolute numbers of Tregs were calculated based on the CD4+CD25+Foxp3+ cells percent-age of CD3+CD4+ cells and the absolute numbers of CD3+CD4+ cells per microliter. RESULTS: The mean number of CD4+CD25+Foxp3+ cells per microliter in colorectal cancer patients was 15.7 (SD: 21.8), for gastric cancer patients 12.2 (SD: 14.3), and for controls 17.5 (SD: 11.4). The absolute number of Tregs was significantly lower in gastric cancer patients than in controls (P = 0.026). There was no statistically significant difference for gastric vs colorectal cancer or colorectal cancer vs controls. The absolute number of Tregs was also significantly depressed in N+ vs Ncancer patients [22.0 (27.7) vs 10.1 (9.0), P = 0.013], and in the subgroup of gastric cancer patients [30.3 (27.6) vs 9.6 (8.0), P = 0.003]. No statistical difference was observed in the proportion of Tregs in the CD4+ population between the groups. CONCLUSION: The absolute number of Tregs in peripheral blood of gastric cancer but not colorectal cancer patients was significantly decreased in comparison with that in healthy controls. | Antoni M Szczepanik Maciej Siedlar Marek Sierzega Dominika Goroszeniuk Karolina Bukowska-Strakova Antoni Czupryna Jan Kulig | 2011 | World Journal of Gastroenterology2011，17，3： | 5 |
| 4 | Design and synthesis by redox polymerization of a bio-based carboxylic elastomer for green tire显示文摘Poly(dibutyl itaconate-co-isoprene-co-methacrylic acid)(PDIM) elastomer was designed and synthesized by redox emulsion polymerization under mild conditions. PDIM has high molecular weight, relatively high yield, and low glass transition temperature(Tg). The structure of PDIM was determined by FTIR and NMR, and the carboxyl content was obtained by titration in a non-proton solvent. Tensile strength and elongation at break increased with increasing carboxyl content. In addition, the interaction between PDIM and silica was elucidated by rubber process analyzer(RPA) and TEM, and the results showed that the silica-PDIM interaction was strong, but the silica-silica interaction was weak. | Xinxin Zhou Runguo Wang Weiwei Lei He Qiao Haoling Ji Liqun Zhang Kuo-Chih Hua Joseph Kulig | 2015 | Science China Chemistry2015，58，10： | 4 |
| 5 | The immunomodulating enteral nutrition in malnourished surgical patients – A prospective, randomized, double-blind clinical trial显示文摘 | Stanislaw Klek Marek Sierzega Piotr Szybinski Kinga Szczepanek Lucyna Scislo Elzbieta Walewska Jan Kulig | 2010 | Clinical Nutrition2010，，3： | 2 |
| 6 | Glucagon-like peptide-1 receptor imaging with [Lys 40 (Ahx-HYNIC- 99m Tc/EDDA)NH 2 ]-exendin-4 for the detection of insulinoma显示文摘 | Anna Sowa-Staszczak Dorota Pach Renata Miko?ajczak Helmut M?cke Agata Jabrocka-Hybel Agnieszka Stefańska Monika Tomaszuk Barbara Janota Aleksandra Gilis-Januszewska Maciej Ma?ecki Grzegorz Kamiński Aldona Kowalska Jan Kulig Andrzej Matyja Czes?aw Osuch Al | 2013 | European Journal of Nuclear Medicine and Molecular Imaging2013，，4： | 2 |
| 7 | Regulation of the release and function of tumor cell-derived soluble CD44 显示文摘 | Cichy J Kulig P Pure E | 2005 | Biochim Biophys Acta2005，1745，1： | 1 |
| 8 | The role of structure activity relationship studies in the search for new GABA uptake inhibitors显示文摘 | Kulig K Szwaczkiewicz M | 2008 | Mini Rev Med Chem2008，8，12： | 1 |
| 9 | An evidence-based review of important issues concerning neonatal hyperbilirubinemia显示文摘 | Ip S Chung M Kulig J O'Brien Sege R Glicken S | 2004 | Pediatrics2004，114，1： | 1 |
| 10 | Intraoperative ultrasonography in detecting and assessment of eolorectal liver metastases 显示文摘 | Kulig J Popiela T Klek S | 2007 | Stand J Surg2007，96，1： | 1 |
| 11 | Transforming growth factor beta, transforming growth factor beta receptor II , and p27Kip 1 expression in nontumorous and neoplastic human pituitaries显示文摘 | Jin L Qian X Kulig E | 1997 | AmJ Pathol1997，151，2： | 1 |
| 12 | Evaluation of adjuvant chemo-therapy irinotecan + 5 -fluorouracil + leucovorine in advanced colorectal cancer显示文摘 | Kulig J Pop iela T Richter P | 2007 | Acta Chir Belg2007，107，3： | 1 |
| 13 | Usefulness of soluble ICAM-1measurements for the evaluation of the disease activity and efficiency of thera-py in patients w ith infiltrative Graves’ophthalmopathy显示文摘 | Kulig G Pilarska K Kulig J Krzyzanow ska-Sw iniarska B Robac-zyk M Baraniak A | 2002 | Pol Arch Med Wew n2002，108，6： | 1 |
| 14 | Liver cell therapy and tissue engineering for transplantation显示文摘 | Vacanti JP Kulig KM | 2014 | Semin Pediatr Surg2014，23，3： | 1 |
| 15 | Development of seasonal allege rhinitis during the first 7 years of life显示文摘 | Kulig M Klettke U Wuhn V | 2000 | J Allergy Clin Immunol2000，106，5： | 1 |
| 16 | The value of imaging techniques in the staging of pancreatic cancer显示文摘 | Kulig J Popiela T Zajac A | 2005 | Surg Endosc2005，19，3： | 1 |
| 17 | Risk factors for erosive esophagitis:a multivariate analysis based on the proGERD study initiative显示文摘 | Labellz J Jaspersen D Kulig M | 2004 | Am J Gastroenterology2004，99，3： | 1 |
| 18 | Long-term results of surgery for early gastric cancer显示文摘 | Popiela T Kulig J Kolodziejczyk P | 2002 | Br J Surg2002，89，8： | 1 |
| 19 | Adjuvant chemotherapy with etoposide, adriamycin and cisplatin compared with surgery alone in the treatment of gastric cancer: a phase III randomized, multicenter, clinical crial 显示文摘 | Kulig J Kolodziejczyk P Sierzega M | 2010 | Oncology2010，78，3： | 1 |
| 20 | Chemerin activation by serine proteases of the coagulation,fibrinolytic,and inflammatory cascades显示文摘 | Zabel BA Allen SJ Kulig P | 2005 | J Biol Chem2005，280，34： | 1 |
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