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| 1 | Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage显示文摘To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat(SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centi Morgan(c M) of the cotton AD genome, with an average marker interval of 2.23 c M. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci(QTLs) for lint percentage(LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality. | Yuzhen Shi Wentan Li Aiguo Li Ruihua Ge Baocai Zhang Junzhi Li Guangping Liu Junwen Li Aiying Liu Haihong Shang Juwu Gong Wankui Gong Zemao Yang Feiyü Tang Zhi Liu Weiping Zhu Jianxiong Jiang Xiaonan Yu Tao Wang Wei Wang Tingting Chen Kunbo Wang Zhengsheng Zhang Youlu Yuan | 2015 | Journal of Integrative Plant Biology2015，57，5： | 7 |
| 2 | Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum显示文摘Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of salinity.Soil salt-alkalization leads to damage in cotton growth and a decrease in yields.Hyperosmolality-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration.Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants.However,the molecular nature of the corresponding calcium ion channels remains unclearly.In this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse genetics.Result:A total of 35,21 and 22 OSCA genes were identified in G.hirsutum,G.arboreum,and G.raimondii genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic relationship.Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) domain.Moreover,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress.Furthermore,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants.Conclusion:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton.These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes,thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions. | YANG Xiu XU Yanchao YANG Fangfang MAGWANGA Richard Odongo CAI Xiaoyan WANG Xingxing WANG Yuhong HOU Yuqing WANG Kunbo LIU Fang ZHOU Zhongli | 2019 | Journal of Cotton Research2019，2，2： | 7 |
| 3 | Designations for individual genomes and chromosomes in Gossypium显示文摘Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics,and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression. | WANG Kunbo WENDEL Jonathan F. HUA Jinping | 2018 | Journal of Cotton Research2018，1，1： | 6 |
| 4 | Component characteristics of organic matter in hydrothermal barnacle shells from Southwest Indian Ridge显示文摘In 2008–2009,hydrothermal barnacle and sediment samples were collected from the Southwest Indian Ridge during a survey of the China Ocean Mineral Resources R&D Association(COMRA).Samples were analyzed by gas chromatography-mass spectrometer(GC-MS),revealing the main organic constituents of hydrothermal barnacle and sediment to be fatty acids and alkylbenzenes.N-alkanes which possessed obvious even carbon advantage were also detected in hydrothermal sediment.The high concentrations of aromatic compounds might be the result of macromolecular thermal alteration.Microorganism in the submarine hydrothermal ecosystem,especially those related to sulfur metabolism,might be the source of the high concentrations of fatty acids detected in these samples.In high temperature and high pressure hydrothermal environments,n-alkanes which possessed obvious even carbon advantage might originate from thermal alteration of carboxylic acids and other lipid compounds. | HUANG Xin ZENG Zhigang CHEN Shuai YIN Xuebo WANG Xiaoyuan ZHAO Huijing YANG Baoju RONG Kunbo MA Yao | 2013 | Acta Oceanologica Sinica2013，32，12： | 6 |
| 5 | Abundance and Distribution of Fatty Acids in Sediments of the South Mid-Atlantic Ridge显示文摘Sediment samples obtained from the South Mid-Atlantic Ridge were studies by gas chromatography-mass spectrometer(GC-MS) for the abundance and distributions of total fatty acids(TFAs). Approximately 34 fatty acids were identified, with the chain-lengths ranging from C12 to C30. The total concentrations of TFAs(∑TFA) ranged from 7.15 to 30.09 μg g-1 dry sediment, and ∑TFA was weakly correlated with bitumen content(R2 = 0.69). The ∑TFA of samples around hydrothermal areas were significantly higher than that of samples away from hydrothermal areas, indicating intense primary production and large biomass in the hydrothermal areas, and suggesting a close relationship between hydrothermal activity and ∑TFA of samples. The characteristics of the TFA composition in the present study are rich in monounsaturated fatty acids and lacking in polyunsaturated fatty acids, and the ratios between the concentrations of monounsaturated fatty acids and ΣTFAs in samples close to the hydrothermal areas, are about 0.8, but for samples far from the hydrothermal areas, they are only about 0.5. Several fatty acids(e.g., a/i C15:0 and C16:1ω7), which are signature biomarkers for sulfur-metabolizing bacteria, show the same distribution trend as ∑TFA of samples, further highlighting the close relationship between fatty acid content and hydrothermal activity and/or hydrothermal communities. The metabolic activities of hydrothermal communities, especially those of microorganisms, are likely the main source of fatty acids in samples. | HUANG Xin ZENG Zhigang CHEN Shuai YIN Xuebo WANG Xiaoyuan MA Yao YANG Baoju RONG Kunbo SHU Yunchao JIANG Tao | 2015 | Journal of Ocean University of China2015，14，2： | 4 |
| 6 | Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH显示文摘Gossypium mustelinum ((广告) 4 ) 是在 Gossypium 的五 disomic 种之一。三 45S ribosomal DNA (rDNA ) loci 被检测在(广告) 4 作为探针与 Gossypium D 染色体种类的 genomic DNA (gDNA ) 作为探查,和三个更明亮的信号与 45S rDNA 被检测。尺寸和这些更明亮的信号的地点与作为探查与 45S rDNA 检测的那些一样,并且被称为 GISH -- 也不。他们之一是超级专业的,它说明了因为在染色体 3,和另外的二点关于它在中期的染色体的一个一半被定位的事实在染色体次要、定位 5 和 9 分别地。所有 GISH 也不位于一个亚染色体染色体,从另外的四 allopolyploid 棉花种分开。GISH -- 也没作为探查,然而并非 A 与 D 染色体种类被检测。极大地反常尺寸和地点(广告) 4 NORs 或 GISH 也不为列在后面的 45S rDNA 多样化(广告) 显示可能的机制 4 种形成。GISH 紧张和 GISH 的比较 -- 有 gDNA 的生产也不在 A 和 D 染色体表演之间探查更好的关系(广告) 4 与一个染色体。最短 G 的一个亚染色体的二个染色体。mustelinum 比 D 亚染色体染色体的最长的染色体短。因此,最长, tetraploid 棉花的 13 个染色体作为一个亚染色体正在被分类,当时更突然, 13 个染色体作为 D 亚染色体正在被分类在传统细胞发生并且 karyotype 分析不能是完全正确的。 | Qiong Wu Fang Liu Shaohui Li Guoli Song Chunying Wang Xiangdi Zhang Yuhong Wang David Stelly Kunbo Wang | 2013 | Journal of Integrative Plant Biology2013，55，7： | 4 |
| 7 | Smectite formation in metalliferous sediments near the East Pacific Rise at 13°N显示文摘A 43 cm long E271 sediment core collected near the East Pacific Rise(EPR) at 13°N were studied to investigate the origin of smectite for understanding better the geochemical behavior of hydrothermal material after deposition.E271 sediments are typical metalliferous sediments. After removal of organic matter, carbonate, biogenic opal,and Fe-Mn oxide by a series of chemical procedures, clay minerals(<2 μm) were investigated by X-ray diffraction,chemical analysis and Si isotope analysis. Due to the influence of seafloor hydrothermal activity and close to continent, the sources of clay minerals are complex. Illite, chlorite and kaolinite are suggested to be transported from either North or Central America by rivers or winds, but smectite is authigenic. It is enriched in iron, and its contents are highest in clay minerals. Data show that smectite is most likely formed by the reaction of hydrothermal Fe-oxyhydroxide with silica and seawater in metalliferous sediments. The Si that participates in this reaction may be derived from siliceous microfossils(diatoms or radiolarians), hydrothermal fluids, or detrital mineral phases. And their δ30 Si values are higher than those of authigenic smectites, which implies that a Si isotope fractionation occurs during the formation because of the selective absorption of light Si isotopes onto Feoxyhydroxides. Sm/Fe mass ratios(a proxy for overall REE/Fe ratio) in E271 clay minerals are lower than those in metalliferous sediments, as well as distal hydrothermal plume particles and terrigenous clay minerals. This result suggests that some REE are lost during the smectite formation, perhaps because their large ionic radii of REE scavenged by Fe-oxyhydroxides preclude substitution in either tetrahedral or octahedral lattice sites of this mineral structure, which decreases the value of metalliferous sediments as a potential resource for REE. | RONG Kunbo ZENG Zhigang YIN Xuebo CHEN Shuai WANG Xiaoyuan QI Haiyan MA Yao | 2018 | Acta Oceanologica Sinica2018，37，9： | 4 |
| 8 | Identification of QTLs and candidate genes for physiological traits associated with drought tolerance in cotton显示文摘Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its cultivation.Effects of drought stress are estimated to affect more than 50%of the cotton growing regions.To elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from G.tomentosum,a drought tolerant donor parent and G.hirsutum which is highly susceptible to drought stress.Results:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 populations.The map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,respectively.Thirty stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At subgenome.Eighty-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll content.The genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined genes.The genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in plants.Through RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in cotton.Wild cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton cultivars.The detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,G.tomentosum,showed the significant genes harbored by the wild progenitors which can be exploited in developing more robust cotton genotypes with diverse tolerance levels to various environmental stresses.Conclusion:This was the first study involving genome wide association mapping for drought tolerance traits in semi wild cotton genotypes.It offers an opportunity for future exploration of these genes in developing highly tolerant cotton cultivars to boost cotton production. | MAGWANGA Richard Odongo LU Pu KIRUNGU Joy Nyangasi CAI Xiaoyan ZHOU Zhongli AGONG Stephen Gaya WANG Kunbo LIU Fang | 2020 | Journal of Cotton Research2020，3，1： | 2 |
| 9 | Component characteristics of polycyclic aromatic compounds in sediments of the South Mid-Atlantic Ridge显示文摘10 samples of sediments obtained from the South Mid-Atlantic Ridge were measured for the abundances and distributions of polycyclic aromatic compounds(PAHs). The total concentrations of PAHs(∑PAHs) ranged from 2.768 to 9.826 μg/g dry sediment. The ∑PAHs was higher in sample 22V-TVG10 and sample 26V-TVG05 which were close to hydrothermal fields, with the lowest value in sample 22V-TVG14 which was farthest from hydrothermal fields, suggesting a probable hydrothermal origin of ∑PAHs of samples. Approximately nine kinds of PAHs were identified, and low molecular mass tricyclic and tetracyclic aromatic compounds were predominant in the samples. The concentrations of fluoranthene which were typical as hydrothermal alteration compounds were the highest among PAHs with dry weight between 0.913–3.157 μg/g. The phenanthrene homologue was most abundant in the samples, and the ratios between parent phenanthrene and methylphenanthrene which probably reflected the degree of hydrothermal alteration ranged from 0.097 to 1.602. The sample 22V-TVG10 possessing a maximum ratio value showed the intense influence of the hydrothermal alteration on this sample, which might further imply that PAHs in sediments were mainly derived from the hydrothermal alteration. | HUANG Xin ZENG Zhigang CHEN Shuai YIN Xuebo WANG Xiaoyuan MA Yao YANG Baoju RONG Kunbo SHU Yunchao JIANG Tao | 2014 | Acta Oceanologica Sinica2014，33，11： | 2 |
| 10 | Mesomorphic behavior of new benzothiazole liquid crystals having Schiff base linker and terminal methyl group显示文摘heterocycles 的一个相应系列, 6-methyl-2-(4-alkoxybenzylidenamino ) benzothiazoles,被综合并且描绘了使用英尺红外, 1H 和 13C NMR 和集体度谱的分析。二形互变的 nematic 阶段为更矮的成员被观察。近晶的 A 阶段仅仅向前从 octyloxy 衍生物出现了。在 benzothiazole 碎片和 Schiff 基础连接的终端甲基组影响了现在的系列的具有由中胚叶发达的行为。 | Sie Tiong Ha Kok Leei Foo Hong Cheu Lin Masato M.Ito Kazuma Abe Kenji Kunbo S.Sreehari Sastry | 2012 | Chinese Chemical Letters2012，23，7： | 2 |
| 11 | Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum显示文摘Gossypium herbaceum var. africanum is the only wild cotton species within the cultivated diploid G. herbaceum. As the A sub-genome donor of tetraploid cotton, it is characterized by its resistance to insects, diseases, and other adversities. We have constructed the first bacterial artificial chromosome library (BAC) for G. herbaceum var. africanum. With high quality and broad coverage, this library includes 75000 clones, with an average insert size of about 115 kb and fewer than 4% empty clones. Our library is approximately five-fold the size of the A-genome (1667 Mb) and it provides 99.3% probability for isolating genes of interest or their sequences. Using nine SSR markers that are located on five different chromosomes and linked with resistance to Verticillium wilt, seven of nine could amplify the 40 superpools and got 1-14 hits. Because of its moderate wide coverage and relative large insert size, this library will be an important genomic resource for classifying and analyzing the evolution of cotton species, as well as for isolating disease-resistance genes and control elements. | GAO HaiYan WANG XingFen LIU Fang PENG RenHai ZHANG Yan CHENG Hua MA ZhiYing WANG KunBo | 2013 | Chinese Science Bulletin2013，58，26： | 1 |
| 12 | The draft genome of a diploid cotton Gossypium mimondii显示文摘 | Wang Kunbo Wang Zhiwen Li Fuguang | 2012 | Nature2012，44，： | 1 |
| 13 | The draftgenome of a diploid cotton gossypium raimondii 显示文摘 | Kunbo Wang Zhiwen Wang Fuguang Li Wuwei Ye Junyi Wang Guoli Song Zhen Yue Lin Cong Haihong Shang Shilin Zhu Changsong Zou Qin Li Youlu Yuan Cairui Lu Hengling Wei Caiyun Gou Zequn Zheng Ye Yin Xueyan Zhang Kun Liu Bo Wang Chi Song Nan Shi Russell J Kohel Richard G Percy John Z Yu Yu-xian Zhu Jun Wang Shuxun Yu | 2012 | Nat Genet2012，，44： | 1 |
| 14 | Genome se- quence of the cultivated cotton Gossypium arboreum显示文摘 | Li Fuguang Fan Guangyi Wang Kunbo | 2014 | Nature2014，46，6： | 1 |
| 15 | Analysis of chemical components in oolong tea in relation to perceived quality 显示文摘 | Kunbo WANG Fang LIU Zhonghua LIU | 2010 | Interna- tional Journal of Food Science and Technology2010，45，： | 1 |
| 16 | Analysis of chemical components in oolong tea in relation to perceived quality 显示文摘 | Wang Kunbo Liu Fang Liu Zhonghua | 2010 | Int J Food Sci Teeh2010，45，5： | 1 |
| 17 | The draft genome of a diploid cotton Gossypium raimondii显示文摘 | Wang Kunbo Wang Zhiwen Li Fuguang | 2012 | Nature Genetics2012，44，10： | 1 |
| 18 | The draft genome of a diploid cotton Gossypium raimondii显示文摘 | Kunbo Wang Zhiwen Wang Fuguang Li | | 0，，10： | 1 |
| 19 | Differential mRNA fingerprinting by preferential amplification of coding sequences 显示文摘 | Bruno Fuchs Kunbo Zhang Mark E | 2000 | Gene2000，，258： | 1 |
| 20 | The draft genome of a diploid cotton Gossypium raimondii显示文摘 | Wang Kunbo Wang Zhiwen Li Fuguang | 2012 | Nature Genetics2012，44，10： | 1 |
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