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| 1 | Loss of ARID1A Promotes Hepatocellular Carcinoma Progression via Up-regulation of MYC Transcription显示文摘Background and Aims:AT-rich interactive domain-containing protein 1A(ARID1A)is frequently mutated or deficient in hepatocellular carcinoma(HCC).However,the role of ARID1A in HCC remains unclear.Therefore,the biological role of ARID1A in HCC was evaluated and a potential mechanism was investigated.Methods:Arid1a was knocked out in the livers of mice using the CRISPR/Cas9 system delivered by hydrodynamic tail vein injection.The development of HCC was observed in different mouse models.The correlation of ARID1A and prognosis in patients with HCC was analyzed using cBioPortal.The effect of ARID1A on cell proliferation was assessed by MTT assay following the manipulation of candidate genes.Results:ARID1A deficiency alone did not cause HCC in mice,but knockout of ARID1A accelerated liver tumorigenesis in response to diethylnitrosamine(DEN)or when a combination knockout of phosphatase and tensin homolog(Pten)plus tumor protein P53(p53)was introduced.ARID1A mutations were associated with a poorer prognosis in HCC patients.The mRNA level of MYC was significantly higher in patients with an ARID1A mutation compared to those without a mutation.Ectopic expression of ARID1A inhibited HCC cell proliferation.ARID1A knockout increased HCC cell growth and resulted in disruptions to DNA damage repair and apoptosis following radiation stress.Furthermore,mechanistic studies revealed that ARID1A inhibited the proliferation of HCC cells via transcriptional down-regulation of MYC.Conclusions:These results describe ARID1A as a tumor suppressor in the liver.A deficiency in ARID1A predicts worse survival in HCC patients and promotes HCC progression via up-regulation of MYC transcription. | Yao Xiao Guodong Liu Xiwu Ouyang Denggao Zai Jixiang Zhou Xiaoli Li Qi Zhang Jie Zhao | 2021 | Journal of Clinical and Translational Hepatology2021，9，4： | 2 |
| 2 | Curve modeling with constrained B-spline wavelets 显示文摘 | Li Denggao Qin Kaihuai Sun Hanqiu | 2005 | Computer Aided Geometric2005，22，1： | 1 |
| 3 | Curve modeling with constrained B-spline wavelets显示文摘 | Li Denggao Qin Kaihuai Sun Hanqiu | 2005 | Computer Aided Geometric Design2005，22，1： | 1 |
| 4 | Curve modeling with constrained B-spline wavelets显示文摘 | LI DENGGAO QIN KAIHUAI SUN HANQIU | 2005 | Computer Aided Geometric Design2005，22，1： | 1 |
| 5 | NO_(x) and H_(2)S formation in the reductive zone of air-staged combustion of pulverized blended coals显示文摘Low NO_(x) combustion of blended coals is widely used in coal-fired boilers in China to control NO_(x) emission;thus,it is necessary to understand the formation mechanism of NO_(x) and H2S during the combustion of blended coals.This paper focused on the investigation of reductive gases in the formation of NO_(x) and H2S in the reductive zone of blended coals during combustion.Experiments with Zhundong(ZD)and Commercial(GE)coal and their blends with different mixing ratios were conducted in a drop tube furnace at 1200℃–1400℃with an excessive air ratio of 0.6–1.2.The coal conversion and formation characteristics of CO,H_(2)S,and NO_(x) in the fuelrich zone were carefully studied under different experimental conditions for different blend ratios.Blending ZD into GE was found to increase not only the coal conversion but also the concentrations of CO and H2S as NO reduction accelerated.Both the CO and H2S concentrations inblended coal combustion increase with an increase in the combustion temperature and a decrease in the excessive air ratio.Based on accumulated experimental data,one interesting finding was that NO and H2S from blended coal combustion were almost directly dependent on the CO concentration,and the CO concentration of the blended coal combustion depended on the single char gasification conversion.Thus,CO,NO_(x),and H2S formation characteristics from blended coal combustion can be well predicted by single char gasification kinetics. | Jinzhi CAI Dan LI Denggao CHEN Zhenshan LI | 2021 | Frontiers in Energy2021，15，1： | 0 |
| 6 | Bayesian parameter estimation of SST model for shock wave-boundary layer interaction flows with different strengths显示文摘The Shock Wave-Boundary Layer Interaction(SWBLI)flow generated by compression corner widely occurs in engineering.As one of the primary methods in engineering,the Reynolds Averaged Navier-Stokes(RANS)methods usually cannot correctly predict strong SWBLI flows.In addition to the defects of the eddy viscosity assumption,the uncertainty of the closure coeffi-cients in RANS models often significantly impacts the simulation results.This study performs para-metric sensitivity analysis and Bayesian calibration on the closure coefficients of the Menter k-x Shear-Stress Transport(SST)model based on the SWBLI with different strengths.Firstly,the para-metric sensitivity on prediction results is analyzed using the Sobol index.The results indicate that the Sobol indices of wall pressure and skin friction exhibited opposite fluctuation trends with the increase of SWBLI strength.Then,the Bayesian uncertainty quantification method is adopted to obtain the posterior probability distributions and Maximum A Posteriori(MAP)estimates of the closure coefficients and the posterior uncertainty of the Quantities of Interests(QoIs).The results indicate that the prediction ability for strong SWBLI of the SST model is significantly improved by using the MAP estimates,and the relative errors of QoIs are reduced dramatically. | Denggao TANG Jinping LI Fanzhi ZENG Yao LI Chao YAN | 2023 | Chinese Journal of Aeronautics2023，36，4： | 0 |
| 7 | 5-Demethylnobiletin and its major metabolites:efficient preparation and mechanism of their anti-proliferation activity in HepG2 cells显示文摘5-Demethylnobile tin(5-DMN),a hydroxylated polymethoxyflavone(OH-PMF)identified in aged citrus peels,has demonstrated health benefiting effects in previous studies.5-DMN undergoes biotransformation in vivo,yielding 5,3’-didemethylnobiletin(5,3’-DDMN),5,4’-didemethylnobiletin(5,4’-DDMN)and5,3’,4’-tridemethylnobiletin(5,3’,4’-TDMN).However,the anti-cancer effects of 5-DMN and its in vivo metabolites against HepG2 cells remain unclear.In this study,an efficient chemical synthetic method was developed to obtain 5-DMN and its 3 metabolites,and their molecular structures were confirmed by;H NMR and LC-MS.Cytotoxicity,cell cycle arrestment,apoptosis and caspase-3 expression were investigated to evaluate the anti-liver cancer effects of these OH-PMFs on HepG2 cells.The results showed that all 4 compounds inhibited the proliferation of HepG2 cells in a concentration-dependent manner.Their anti-proliferative activity was exerted through inducing G2/M phase arrestment,cell apoptosis and promoting expression of a key apoptotic protein called cleaved caspase-3.Our results indicated that 5,3’-DDMN and5,3’,4’-TDMN showed a stronger inhibitory activity on cell proliferation than 5-DMN,followed by 5,4’-DDMN.The expression of cleaved caspase-3 was the highest in cells treated with 5,4’-DDMN,implying that the apoptosis induced by other OH-PMFs might be mediated by other apoptotic execution proteins.Our research reveals the application potential and scientific evidence for the production and functionality of OH-PMFs. | Yanping Xin Ting Zheng Man Zhang Ruiqiang Zhang Siyue Zhu Dongli Li Denggao Zhao Yanyan Ma Chi-Tang Ho Qingrong Huang | 2022 | Food Science and Human Wellness2022，11，5： | 0 |
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