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| 1 | Function of apoptosis and expression of the proteins Bcl-2,p53 and C-myc in the development of gastric cancer显示文摘INTRODUCTIONIn China ,the incidence and mortality of gastric cancer rank the second among all cancers. Recent development of cancer [1-20].The aim of this study was investigat the insight of apoptosis and bcl-2, p53 and C-myc protein expression in the development of gastric cancer . | An Gao Xu Shao Guang Li Ji Hong Liu Ai Hua Gan Research Laboratory of Digestive Disease,Huizhou Central People’s Hospital,Huizhou 516001,Guangdong Province,ChinaDr.An Gao Xu graduated from Guangdong Medical College in 1984.He is an associate physician-in-chief,specializing in the research and treatment of gastrointestinal and liver tumors.He has published 24 papers and 1 book. | 2001 | World Journal of Gastroenterology2001，7，3： | 91 |
| 2 | Changes of NF-kB,p53,Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells:role of reactive oxygen species显示文摘AIM: To identify whether JTE-522 can induce apoptosis inAGS cells and ROS also involved in the process, and toinvestigate the changes in NF-kB, p53, bcl-2 and caspase in the apoptosis process.METHODS: Cell culture, MTT, Electromicroscopy, agarosegel electrophoresis, lucigenin, Western blot andelectrophoretic mobility shift assay (EMSA) analysis wereemployed to investigate the effect of JTE-522 on cellproliferation and apoptosis in AGS cells and relatedmolecular mechanisms.RESULTS: JTE-522 inhibited the growth of AGS cells andinduced the apoptosis. Lucigenin assay showed the generationof ROS in cells under incubation with JTE-522. The inc eareasedROS generation might contribute to the induction of AGS cellsto apoptosis. EMSA and Westem blot revealed that NF-kBactivity was almost completely inhibited by preventing thedegradation of IkBα. Additionally, by using Western blot weconfirmed that the level of bcl-2 was decreased, whereas p53showed a great increase following JTE-522 treatment. Theirchanges were in a dose-dependent manner.CONCLUSION: These findings suggest that reactive oxygenspecies, NF-kB, p53, bcl-2 and caspase-3 may play animportant role in the induction of apoptosis in AGS cellsafter treatment with JTE-522. | Hong-Liang Li Xiao-Hong Li Yan-Qing L Chun-Ling Ye Xian-Da Ren,Department of Pharmacology,Jinan University Pharmacy College,Guangzhou 510632,Guangdong,China Dan-Dan Chen,Department of Cardiology,First Affiliated Hospital,Zhongshan University,Guangzhou 510089,Guangdong,China Hai-Wei Zhang,Department of Pathology,Jinan University Medical College,Guangzhou 510632,Guangdong,China | 2002 | World Journal of Gastroenterology2002，8，3： | 58 |
| 3 | Changes in the level of serum liver enzymes after laparoscopic surgery显示文摘AIM: The purpose of this study was to investigate the effectof laparoscopic surgery on liver function in humans and thepossible mechanisms behind such effect.METHODS: Blood samples from 286 patients whounderwent laparoscopic cholecystectomy (LC) and 40patients who underwent open cholecystectomy (OC) weretested for liver function by measuring the level of serumalanine aminotrasferase (ALT) and aspartate aminotrasferase(AST) before and after the operations. The same tests werealso applied to 18 laparoscopic colorectal cancer resection(LCR) patients and 23 open colorectal cancer resection (OCR)patients to determine whether CO2 pneumoperitoneum couldalter the serum liver enzymes.RESULTS: The level of serum ALT and AST increasedsignificantly during the first 48 hours post operations in bothLC and LCR patients. However, no significant change of theserum liver enzymes was detected in both OC and OCRpatients. As a result, there was statistically significantdifference in change of both ALT and AST levels betweenLC and OC patients and LCR and OCR patients, respectively.By the 7th day post operation, the level of both enzymesreturned to normal values in LC, OC and OCR patients exceptLCR patients whose enzymes remained at a higher level.CONCLUSION: Transient elevation of hepatic transaminasesoccurred after laparoscopic surgery. The major causativefactor seemed to be the CO2 pneumoperitoneum. In mostof the laparoscopic surgery patients, the transient elevationof serum liver enzymes showed no apparent clinicalimplications. However, if preoperative liver function was verypoor, laparoscopic surgery may not be the best choice forthe treatment of patients with certain abdominal diseases. | Min Tan Feng-Feng Xu Jun-Shen Peng Dong-Ming Li Liu-Hua Chen Bao-Jun Lv Zhen-Xian Zhao Chen Huang Chao-Xu Zheng Department of General Surgery, the First Affiliated Hospital of Zhong Shah University, 58 Zhongshan 2 Road Guangzhou 510080, Guangdong Province China | 2003 | World Journal of Gastroenterology2003，9，2： | 42 |
| 4 | Treatment outcomes for different subgroups of nasopharyngeal carcinoma patients treated with intensity-modulated radiation therapy显示文摘Although many studies have investigated intensity-modulated radiation therapy (IMRT) for nasopharyngeal carcinoma (NPC),sample sizes in the reported studies are usually small and different in outcomes in different T and N subgroups are seldom analyzed.Herein,we evaluated the outcomes of NPC patients treated with IMRT and further explored treatment strategy to improve such outcome.We collected clinical data of 865 NPC patients treated with IMRT alone or in combination with chemotherapy,and classified all cases into the following prognostic categories according to different TNM stages:early stage group (T1-2N0-1M0),advanced local disease group (T3-4N0-1M0),advanced nodal disease group (T1-2N2-3M0),and advanced locoregional disease group (T3-4N2-3M0).The 5-year overall survival (OS),local relapse-free survival (LRFS),and distant metastases-free survival (DMFS) were 83.0%,90.4%,and 84.0%,respectively.The early disease group had the lowest treatment failure rate,with a 5-year OS of 95.6%.The advanced local disease group and advanced nodal disease group had similar failure pattern and treatment outcomes as well as similar hazard ratios for death (4.230 and 4.625,respectively).The advanced locoregional disease group had the highest incidence of relapse and death,with a 5-year DMFS and OS of 62.3% and 62.2%,respectively,and a hazard ratio for death of 10.402.Comparing with IMRT alone,IMRT in combination with chemotherapy provided no significant benefit to locoregionally advanced NPC.Our results suggest that the decision of treatment strategy for NPC patients should consider combinations of T and N stages,and that IMRT alone for early stage NPC patients can produce satisfactory results.However,for advanced local,nodal,and locoregional disease groups,a combination of chemotherapy and radiotherapy is recommended. | Sheng-Fa Su 1,2,3,Fei Han 1,2,Chong Zhao 1,2,Ying Huang 1,2,Chun-Yan Chen 1,2,Wei-Wei Xiao 1,2,Jia-Xin Li 1,2 and Tai-Xiang Lu 1,2 1 State Key Laboratory of Oncology in South China,Guangzhou,Guangdong 510060,P.R.China 2 Department of Radiation Oncology,Sun Yat-sen University Cancer Center,Guangzhou,Guangdong 510060,P.R.China 3 Department of Oncology,Guiyang Medical College Hospital,Tumor Hospital of Guizhou,Guiyang,Guizhou 550003,P.R.China. | 2011 | Chinese Journal of Cancer2011，30，8： | 40 |
| 5 | Treatment of large lumbar disc herniation with percutaneous ozone injection via the posterior-lateral route and inner margin of the facet joint显示文摘AIM:To evaluate the effects of percutaneous ozone injection via the posterior-lateral route and inner margin of the facet joint in the treatment of large lumbar disc herniation.METHODS:Fifty-eight patients with large lumbar disc herniation were treated with percutaneous injection of ozone via the posterior-lateral route and inner margin of the facet joint under digital subtraction angiography.Second injections were performed 5 d after the initial injection.All patients were followed up for 6-18 mo.A modified Macnab method was used for assessing clinical outcomes after oxygen-ozone therapy.RESULTS:Successful puncture was obtained in all patients.The overall efficacy was 91.4;the outcome was the excellent in 37 cases(63.8),good in 16 cases(27.6) and fair/poor in 5 cases(8.6) according to the Macnab criteria.No severe complications were found throughout this study.CONCLUSION:Percutaneous intradiscal ozone injection via the posterior-lateral route and inner margin of the facet joint is effective and safe for treatment of large lumbar disc herniation. | Wei Lu,Yan-Hao Li,Xiao-Feng He,Department of Interventional Radiology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong Province,China | 2010 | World Journal of Radiology2010，2，3： | 31 |
| 6 | (-)-Epigallocatechin-3-gallate inhibits growth of gastric cancer by reducing VEGF production and angiogenesis显示文摘AIM: To investigate the effect of (-)-epigallocatechin- 3-gallate (EGCG) on growth of gastric cancer and its possible mechanism. METHODS: Heterotopic tumors were induced by subcutaneously injection of SGC-7901 cells in nude mice. Tumor growth was measured by calipers in two dimensions. Tumor angiogenesis was determined with tumor microvessel density (MVD) by immunohistology. Vascular endothelial growth factor (VEGF) protein level and activation of signal transducer and activator of transcription 3 (Stat3) were examined by Western blotting. VEGF mRNA expression was determined by RT-PCR and VEGF release in tumor culture medium by ELISA. VEGF-induced cell proliferation was studied by MTT assay, cell migration by gelatin modified Boyden chamber (Transwell) and in vitro angiogenesis by endothelial tube formation in Matrigel. RESULTS: Intraperitoneal injection of EGCG inhibited the growth of gastric cancer by 60.4%. MVD in tumor tissues treated with EGCG was markedly reduced. EGCG treatment reduced VEGF protein level in vitro and in vivo. Secretion and mRNA expression of VEGF in tumor cells were also suppressed by EGCG in a dose-dependent manner. This inhibitory effect was associated with reduced activation of Stat3, but EGCG treatment did not change the total Stat3 expression. EGCG also inhibited VEGF-induced endothelial cell proliferation, migration and tube formation. CONCLUSION: EGCG inhibits the growth of gastriccancer by reducing VEGF production and angiogenesis, and is a promising candidate for anti-angiogenic treatment of gastric cancer. | Bao-He Zhu, Wen-Hua Zhan, Zheng-Rong Li, Zhao Wang, Yu-Long He, Jun-Sheng Peng, Shi-Rong Cai, Jin-Ping Ma, Chang-Hua Zhang, Department of Gastrointestinal & Pancreatic Surgery, First Affiliated Hospital, Sun Yat-Sen University Gastric Center of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China | 2007 | World Journal of Gastroenterology2007，13，8： | 30 |
| 7 | 交互联合上的国际进步和评估在都市化和 eco 环境之间完成显示文摘全球都市化正在施加严重应力并且在 eco 环境上有影响深远的影响,并且还在那里存在在二之间的一种复杂非线性的联合关系。关于在都市化和 eco 环境之间的交互联合效果的研究将在下一 10 年里在国际地球系统科学和可持续性科学是学习和边疆的一个流行区域,当也是到国际组织的特别兴趣的一个高优先级的研究话题时。这篇论文系统地对照并且总结在关于交互联合理论的研究取得的国际进步,联合机制,联合法律,联合阀值,联合模型并且联合优化决定支持系统,联合关系。研究显示出交互联合理论包括的那都市化和 eco 环境 Kuznets 曲线理论, telecoupling 理论,行星的边界理论,脚印家庭理论和城市的新陈代谢理论;关于交互联合关系的大多数研究关心单身者 -- 元素联合关系,例如那些在都市化和水之间,土地,空气,气候变化,生态系统和生物多样性;关于交互联合机制和法律的研究的多数集中于五个研究范例,包括联合的人和自然开发和 eco 环境限制包围的系统,复杂聚会生态的系统,城市的生态系统, social-economic-natural 建筑群生态系统,和都市化;交互联合模拟使用 STIRPAT 的多数当模特儿,联合城市的计算机建模的度模型,多代理人系统模型和大数据;并且研究在联合阀值,强制风险和最佳的决定支持的都市化和 eco 环境上被执行了系统。在关于在都市化和 eco 环境之间的交互联合的国际研究的进步的客观评估建议有缺乏的焦点的点和六个区域研究的六主要研究:很多研究在宏观的联合效果上存在,与关于联合效果的城市的凝块和规模的很少研究;可观的研究在单个尺寸的联合效果上存在,与多重尺寸的联合效果上的很少;很多存在在上一对一双 -- 元素联合效果,与 many-to-many 上的很少,多重元素的联合完成;很多在在联合效果的否定反馈上联合效果,和很少的积极反馈上存在;很多在实验联合效果上存在,并且理论联合上的很少完成;很多在使用综合模拟方法上在简单量的方法,和很少的使用上存在。未来研究应该集中于在城市的凝块和 eco 环境联合在都市化之间的效果,空间规模联合效果,多维的联合效果, telecoupling 效果,一对多并且 many-to-many 元素联合效果,和积极、否定的反馈联合效果。也有需要为多元素, -scale,-scenario, 模块和代理人加强动态模型的开发和应用程序综合空间与时间的联合系统并且进一步在联合效果研究改进理论革新并且集成并且形成完全、多样的联合理论系统。 | FANG Chuanglin LIU Haimeng LI Guangdong | 2016 | Journal of Geographical Sciences2016，26，8： | 28 |
| 8 | Fatigue reliability assessment for bridge welded details using long-term monitoring data显示文摘A reliability assessment method of fatigue life based on the long-term monitoring data is developed for welded details in steel box girder,and the application research is presented with examples of welded rib-to-deck details in Runyang Bridges. Firstly the fatigue damage limit-state function is established based on S-N curves and Miner's rule,and the probability distribution characteristics of the coefficients in the function are discussed in detail. The uncertainties in fatigue loading effects are mainly studied based on long-term monitoring data. In the traditional studies,only the uncertainty of equivalent stress range is considered in fatigue reliability assessment. However,stress cycle number is also treated as a random variable in this paper because we know traffic flow every day differs in a thousand ways. Then the optimization method is employed to calculate the fatigue reliability. After studying the changing law of the reliability indices with time and the effect of the randomness of stress cycle number on reliability,the effect of the traffic growth on the reliability is studied. This study shows that the uncertainty in the fatigue life of the welded details can be well studied based on structural health monitoring,so it is necessary to carry out long-term strain monitoring of the welded details for accurate fatigue reliability assessment during the whole service period. | DENG Yang DING YouLiang LI AiQun ZHOU GuangDong | 2011 | Science China(Technological Sciences)2011，54，12： | 24 |
| 9 | Transcription factor EGR-1 inhibits growth of hepatocellular carcinoma and esophageal carcinoma cell lines显示文摘瞄准:抄写因素 EGR-1 (早生长反应 gene-1 ) 在房间生长,区别和开发起一个重要作用。它鉴别了 EGR-1 在一些瘤举办重要转变抑制活动,例如纤维肉瘤,胸癌。这个实验被设计在 hepatocellular 癌(HCC ) 和食道的癌(EC ) 的癌的过程调查 egr-1 的角色,然后在这些肿瘤细胞的生长上估价 EGR-1 的效果。方法:第一,在 HCC 和 EC 的 egr-1 的抄写和表达式,帕拉癌的纸巾和他们的正常对应物部分被检测由在 situ 杂交和免疫组织化学,与正常的人的胸和是的鼠标大脑纸巾积极控制。Egr-1 基因当时是进 HCC 的 transfected (HHCC, SMMC7721 ) 并且没有 egr-1 抄写和表示是在场的 EC (ECa109 ) 房间在线。在裸体老鼠的房间生长速度, FCM 房间周期,板克隆形成和 tumorigenicity 被观察,控制仅仅是有向量的房间线 transfected。结果:很少或没有 egr-1 抄写和表示在 HCC, EC 和正常的肝纸巾被检测。egr-1 的表示在 hepatocellular 帕拉被发现更高癌的织物(抄写水平 P=0.000;表示水平 P=0.143,可能因为在盒子的数字的少数) 并且食道的癌症的 dysplastic 织物(抄写水平 P=0.000;表示水平 P=0.001 ) 。egr-1-transfected HHCC (HCC 细胞线) 的生长率细胞和 ECa109 (EC 细胞线) 细胞比控制的慢得多。S 阶段房间,克隆形成和 tumorigenicity 的比例控制的是比这些显著地低的(减少 45.5% 在 HHCC 房间并且 34.1% 在 ECa109 房间;46.6% 和 41.8% ;80.4% 和 72.6% 分别地) 。关于上述项目 SMMC7721 (HCC ) egr-1-transfected 房间和控制之间没有明显的差别。结论:egr-1 的减少的表示可能在 HCC 和 EC 的癌的过程在正常生长的 dysregulation 起一个作用。transfected HHCC 和 ECa109 细胞的 Egr-1 基因显示出细胞生长和在 SMMC7721 (HCC 细胞线) 的恶意的显型,而是没有抑制细胞的明显的抑制。 | Miao-Wang Hao Li Liu,Department of Internal Medicine,Tangdu Hospital,Xi’an 710038,Shaanxi Province,China Ying-Rui Liang Ming-Yao Wu Huan-Xing Yang,Department of Pathology,Medical College of Shantou University,Shantou 515031,Guangdong Province,China Yan-Fang Liu,Department of Pathology,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 24 |
| 10 | Telomere and telomerase in the initial stage of immortalization of esophageal epithelial cell显示文摘AIM: To search for the biomarker of cellular immortalization,the telomere length, telomerase activity and its subunits incultured epithelial cells of human fetal esophagus in theprocess of immortalization.METHODS: The transgenic cell line of human fetalesophageal epithelium (SHEE) was established with E6 E7genes of bt man papillomavirus (HPV) type 18 in ourlaboratory. Morphological phenotype of cultured SHEE cellsfrom the 6th to 30th passages, was examined by phasecontrast microscopy, the telomere length was assayed bySouthern blot method, and the activity of telomerase wasanalyzed by telomeric repeat amplification protocol (TRAP).Expressions of subunits of telomerase, hTR and hTERT,were assessed by RT-PCR. DNA content in cell cycle wasdetected by flow cytometry. The cell apoptosis wasexamined by electron microscopy (EM) and TUNEL label.RESULTS: SHEE cells from the 6 th to 10 th passagesshowed cellular proliferation with a good differentiation.From the 12 th to the 16 th passages, many senescent andapoptotic cells appeared, and the telomere length sharplyshortened from 23 kb to 17 kb without expression of hTERTand telornerase activity. At the 20 th passage, SHEE cellsovercame the senescence and apoptosis and restored theirproliferative activity with expression of telomerase andhTERT at low levels, but the telomere length shortenedcontinuously to the lowest of 3 kb. After the 30 th passagecells proliferation was restored by increment of cells at S andG2M phase in the cell cycle end telomerase activity expressedat high levels and with maintenance of telomere length.CONCLUSION: At the early stage of SHEE cells, telomeresare shortened without expression of telomerase and hTERTcausing cellular senescence and cell death. From the 20 thto the 30 th passages, the activation of telomerase andmaintenance of telomere length show a progressive processfor immortalization of esophageal epithelial cells. Theexpression of telomerase may constitute a biomarker fordetection of immortalization of cells. | Zhong-Ying Shen Li-Yan Xu Wei-Jia Cai Min-Hua Chen Jian Shen,Department of Tumor Pathology,Medical College of Shantou University,Shantou 515031,Guangdong Province,China En-Min Li,Department of Biochemistry and Molecular Biology,Medical College of Shantou University,Shantou 515031,Guangdong Province,China Yi Zeng,Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China | 2002 | World Journal of Gastroenterology2002，8，2： | 21 |
| 11 | Effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of neural stem cells derived from the cerebral cortex of embryonic mice显示文摘Salvianolic acid B is isolated from Salvia miltiorrhiza,the root of which is widely used as a traditional Chinese medicine to treat stroke.However,little is known about how salvianolic acid B influences growth characteristics of neural stem cells (NSCs).The purpose of the present study was to evaluate the effects of salvianolic acid B on proliferation,neurite outgrowth and differentiation of NSCs derived from the cerebral cortex of embryonic mice using MTT,flow cytometry,immunofluorescence and RT-PCR.It was found that 20 μg mL·1 and 40 μg mL·1 salvianolic acid B had similar effects on proliferation of NSCs,and a suitable concentration of salvianolic acid B increased the number of NSCs and their derivative neurospheres.The growth-promoting activity of salvianolic acid B was dependent on and associated with an accumulation in the G2/S-phase cell population.Salvianolic acid B also promoted the neurite outgrowth of NSCs and their differentiation into neurons.The mRNA for tau,GFAP and nestin were present in differentiating neurospheres induced by salvianolic acid B.However,high-level expression of tau mRNA and low-level expression of GFAP mRNA was detected in differentiated cells,in contrast to the control conditions.This collective evidence indicates that exogenous salvianolic acid B is capable of promoting proliferation of neurospheres and differentiation towards the neuronal lineage in vitro and may act in the proliferation of NSCs and may promote NSC differentiation into neuronal cells. | GUO GuoQing1,LI Bin1,WANG YuanYuan1,SHAN AiJing1,SHEN WeiZai1,YUAN Lin2 & ZHONG ShiZhen2 1Department of Anatomy,Medical College of Jinan University,Guangzhou 510630,China 2Institute of Clinical Anatomy,Key Laboratory of Tissue Construction and Detection of Guangdong Province,Southern Medical University,Guangzhou 510150,China | 2010 | Science China(Life Sciences)2010，53，6： | 21 |
| 12 | Antitumor immunopreventive and immunotherapeutic effect in mice induced by hybrid vaccine of dendritic cells and hepatocarcinoma in vivo显示文摘AIM: To develop atumor vaccine by fusion of H22 hepatocarcinoma cells and DC, and to study its protective and therapeutical effect against H22 cell.METHODS: H22-DC vaccine was produced by PEG fusion of H22 and DC induced by cytokine released from splenic mononuclear cells, sorted by CD11c magnetic microbead marker. It was injected through the tail vein of the mice and the H22-DC oncogenesis was detected in the liver, spleen and lung. In order to study the therapeutical and protective effect of H22-DC against tumor H22, two groups were divided:immune group and therapeutic group. Immune group was further divided into P, D, HD and H subgroups, immunized by PBS, DC, H22-DC and inactivated H22, respectively, and attacked by H22 cell. The tumor size, tumor weight, mice survival time and tumor latent period were recorded and statistically analyzed; Therapeutical group was divided into three subgroups of P, D and HD, and attacked by H22, then treated with PBS, DC, and H22-DC, respectively. Pathology and flow cytometry were also applied to study the mechanism how the H22-DC vaccine attacked on the H22 cell.RESULTS: 1. No oncogenesis was found in spleen, lung and liver after H22-DC injection. 2. Hybrid vaccine immunized mice had strongest CTL activity. 3. In the immune group,latent period was longer in HD subgroup than that in P, H and D subgroup; and tumor size and weight were smaller in HD subgroup than that in P, H and D subgroup. 4. In therapeutic group, tumor size was smaller in HD subgroup than that in P, D subgroup.CONCLUSION: 1. H22-DC tumor vaccine is safe without oncogenesis in vivo. 2. Hybrid vaccine can stimulate potent specific CTL activity against H22.3. H22-DC vaccine has distinctive prophylatic effect on tumor H22 and can inhibit the tumor growth. | Jin-Kun Zhang Jun Li Juan Zhang Hai-Bin Chen Su-Biao Chen Cancer Pathology Laboratory, Shantou University Medical College, Shantou 515031, Guangdong Province, China | 2003 | World Journal of Gastroenterology2003，9，3： | 21 |
| 13 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 14 | Antitumor activities of human dendritic cells derived from peripheral and cord blood显示文摘AIM: To observe the biological specialization of humanperipheral blood dendritic cells (DC) and cord blood derivedDC and its effects on effector cells killing humanhepatocarcinoma cell line BEL-7402 in vitro.METHODS: The DC biological characteristics were detectedwith immunohistochemical and MTT assay. Two antitumorexperimental groups are: peripheral blood DC and cordblood DC groups. Peripheral blood DC groups used LAKcells as the effector cells and BEL-7402 as target cells, whilecord blood DC groups used CTL induced by tumor antigentwice pulsed DC as effector cells and BEL-7402 as targetcells, additional peripheral blood DC and cord blood DC areadded to observe its stimulating activities to effector cells.The effector's cytotoxicity to tumor cells were detected withneutral red colorimetric assay at two effector/target ratios of5:1 and 10: 1.RESULTS: Peripheral blood DC and cord blood DC highlyexpressed HLA-ABC, HLA-DR, HLA-DQ, CD54 and S-100protein. The stimulating activities to lymphocyteproliferation were compared between experimental groups(DC added) and control group (no DC added). In sixexperiment subgroups, the DC/lymphocyte ratio wassequentially 0.25: 100, 0.5: 100, 1: 100, 2: 100, 4: 100 and 8:100, A values(x± s) were 0.75396± 0.009, 0.84916± 0.010,0.90894± 0.012, 0.98371 ± 0.007, 1.01299 ± 0.006 and 1.20384± 0.006 in peripheral blood DC groups and 0.77650 ± 0.005,0.83008± 0.007, 0.92725 ± 0.007, 1.05990 ± 0.010, 1.15583 ±0.011, 1.22983 ± 0.011 in cord blood DC groups. A value was0.59517 ± 0.005 in control group. The stimulating activitieswere higher in experimental groups than in control group ( P< 0.01 ), which were increased when the DC concentrationwas enlarged ( P < 0.01 ). Two differently derived DCs hadthe same phenotypes and similar stimulating activities ( P >0.05). In peripheral blood DC groups, the cytotoxicity (x ±s) of the LD groups (experimental groups) and L groups(control group) was 58.16% ± 2.03% (5: 1), 46.18% ±2.25% (10:l) and 38.13% ± 1.29% (5: 1) and 65.40% ±1.56% (10: 1), respectively; in cord blood DC groups, TDgroups (experimental groups ) and T groups (controlgroups) were 69.71% ± 2.33% (5: 1 ), 77.64% ± 1.94% ( 10:1) a. nd 56.89% ± 1.82% (5:1) and 60.99% ± 1.42% (10:1),respectively. The cytotoxicity activities were enhanced withincreased effector/target ratio ( P < 0.01 ). At the sameeffector/target ratio, the cytotoxicity of experimental groupswas higher than that of control groups ( P < 0.01 ). Thecytotoxicity of cord blood DC groups were higher than ofperipheral blood DC groups ( P < 0.01 ).CONCLUSION: Peripheral blood DC and cord blood DC aremature DCs in morphology and function, both can enhancethe effector cell killing activities to hepetocarcinoma cells.DC pulsed with tumor antigen can induce ligher specific CTLactivity than unpulsed DC. | Jin-Kun Zhang Jun Li Hai-Bin Chen Jin-Lun Sun Yao-Juan Qu Juan-Juan Lu Cancer Pathology Laboratory,Shantou University,Medical College,Shantou 515031,Guangdong Province,China | 2002 | World Journal of Gastroenterology2002，8，1： | 18 |
| 15 | Applications of functionalized ionic liquids显示文摘Recent developments of the synthesis and applications of functionalized ionic liquids (including dual-functionalized ionic liquids) have been highlighted in this review. Ionic liquids are at-tracting attention as alternative solvents in green chemistry, but as more functionalized ILs are pre-pared, a greater number of applications in increasingly diverse fields are found. | LI Xuehui1, ZHAO Dongbin2,3, FEI Zhaofu2 & WANG Lefu1 1. Department of Chemical Engineering, Guangdong Provincial Laboratory of Green Chemical Technology, South China Uni-versity of Technology, Guangzhou 510641, China 2. Swiss Federal Institute of Technology, Lausanne, EPFL, CH-1015 Lausanne, Switzerland 3. PKU Green Chemistry Center, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China | 2006 | Science China Chemistry2006，49，5： | 18 |
| 16 | JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release,membrane translocation of Bax and loss of mitochondrial membrane potential显示文摘瞄准:在 JTE-522-induced apoptosis 调查 mitochondrial 小径的角色并且调查在细胞色素 C 版本, caspase 活动和 mitochondrial 膜潜力(Deltapsim ) 的损失之间的关系。方法:房间文化,数的房间, ELISA 试金, TUNEL,流动 cytometry,西方的污点和 fluorometric 试金被采用在 AGS 房间和相关分子的机制在房间增长和 apoptosis 上调查 JTE-522 的效果。结果:JTE-522 禁止了 AGS 细胞的生长并且导致了 apoptosis。Caspases 8 并且 9 在从 procaspase 和 caspase 活动由劈开产品的外观判定了劈开特定的 fluorogenic 底层的 apoptosis 期间被激活。阐明是否 caspases 的激活 8 和 9 为 apoptosis 正式就职被要求,我们在 apoptosis 上检验了 caspase 特定的禁止者的效果。结果证明 caspase 禁止者显著地禁止了 JTE-522 导致的 apoptosis。另外,与 Rhodamin 123 的举起的减少伴随的细胞色素 C 的 Bax 和 cytosolic 版本的膜 translocation,在 apoptosis 的一个早阶段被检测。而且, Bax translocation,细胞色素 C 版本,和 caspase 9 激活被 Z-VAD.fmk 和 Z-IETD-CHO 堵住。结论:现在的数据显示在 caspases 的激活之间的一个关键协会 8, 9,细胞色素 C 版本, Bax 的膜 translocation, Deltapsim 的损失和在 AGS 房间的 JTE-522-induced apoptosis。 | Hong-Liang Li Xiao-Hong Li Jun-Hua Lü Xian-Da Ren,Department of Pharmacology,Jinan University Pharmacy College,Guangzhou 510632,Guangdong Province,China Dan-Dan Chen,Department of Cardiology,First Affiliated Hospital,Zhongshan University,Guangzhou 510089,Guangdong Province,China Hai-Wei Zhang,Department of Pathology,Jinan University Medical College,Guangzhou 510632,Guangdong Province,China Cun-Chuan Wang,Department of laparoscopic surgery,First Affiliated Hospital,Jinan University Medical College,Guangzhou 510632,Guangdong Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 16 |
| 17 | Influence of whole peptidoglycan of bifidobacterium on cytotoxic effectors produced by mouse peritoneal macrophages显示文摘INTRODUCTIONBifidobacteria are physiologically beneficial bacteria which are perdominant in human intestine ,and possess the most important functions .They play an important role in maintaining microbial balance of the intestine .Furthermore , their presence is thought to be an important indication of health of the body [1-4].Whole peptidoglycan ( WPG) is the major component in the cell wall of bifidobacterium ,which is also a biological responsemodifier with nontoxic side dffcets. | Li Sheng Wang~1 Hui Ming Zhu~1 Dian Yuan Zhou~2 Yu Lin Wang~1 Wan Dai Zhang~2 ~1Departrnent of Gastroenterology,Shenzhen Municipal People’s Hospital,Jinan University of Medical Sciences,Shenzhen 518020,Guangdong Province,China ~2Chinese PLA Institute of Digestion,the First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaLi Sheng Wang graduated and obtained Ph.D,from the First Military Medical University in 1998,now working at Department of Gastroenterology,Shenzhen Municipal People’s Hospital.Jinan University of Medical Sciences.having 35 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 15 |
| 18 | Evaluation of ecosystem services of Chinese pine forests in China显示文摘Evaluation of forest ecosystem services is a hot topic,both in China and at abroad,but it has not yet obtained a consistency of evaluation indicator systems and evaluation methods.Under the framework of evaluation criteria to be implemented for forest ecosystem services,years of consecutive observation data from Long Term Ecological Research Stations affiliated to Chinese Forest Ecosystem Research Network(CFERN),forest resource inventory and public data were applied to carry out a detailed and dynamic evaluation on the physical quantity and value of ecosystem services of Chinese pine forests in China.The results showed that the above services had the total value and unit value of 1144.9640 billion(1.1449640×10 12 )RMB and 52.074 thousand RMB per hectare per year,respectively during the 9th Five-year Plan(1996―2000),and of 1190.5461 billion RMB and 52.101 thousand RMB per hectare per year,respectively,during the 10th Five-year Plan(2001―2005).For Chinese pine forests,water conservation was 40.40 hundred million cubic meters annually,soil conservation was 67 million tons and C fixation 9 million tons annually,production of healthful negative ions was 1.96×10 20 , absorption of SO2 was 5.02 hundred million kilograms and dust-catching was 759.10 hundred million kilograms. Among the 15 provinces of China with Chinese pine forests,the biggest beneficiary from ecosystem services was Liaoning Province;while Hunan Province was the smallest beneficiary between the 9th Five-year Plan. | GUO Hao ,WANG Bing,MA XiangQian,ZHAO GuangDong&LI ShaoNing Research Institute of Forest Ecology Environment and Protection of CAF,Key Laboratory of Forest Ecological Environment of State Forestry Administration,Beijing 100091,China | 2008 | Science China(Life Sciences)2008，51，7： | 14 |
| 19 | Roux-en-Y gastric bypass promotes expression of PDX-1 and regeneration of β-cells in Goto-Kakizaki rats显示文摘AIM:To study the effects of Roux-en-Y gastric bypass(RYGB) on the expression of pancreatic duodenal homeobox-1(PDX-1) and pancreatic β-cell regeneration/neogenesis,and their possible mechanisms in diabetics.METHODS:Three groups of randomly selected nonobese diabetic Goto-Kakizaki(GK) rats were subjected to RYGB,sham-RYGB and sham-operation(sham-op) surgery,respectively.The rats were euthanized at postoperative 1,2,4 and 12 wk.Their pancreases were resected and analyzed using reverse transcription polymerase chain reaction to detect the mRNA of PDX-1.Anti-PDX-1 immunohistochemical(IHC) staining and Western blotting were used to detect the protein of PDX-1.Double IHC staining of anti-Brdu and-insulin was performed to detect regenerated β-cells.The index of double Brdu and insulin positive cells was calculated.RESULTS:In comparison with sham-RYGB and sham-op groups,a significant increase in the expressions of PDX-1 mRNA in RYGB group was observed at all experimental time points(1 wk:0.378 ± 0.013 vs 0.120 ± 0.010,0.100 ± 0.010,F = 727.717,P < 0.001;2 wk:0.318 ± 0.013 vs 0.110 ± 0.010,0.143 ± 0.015,F = 301.509,P < 0.001;4 wk:0.172 ± 0.011 vs 0.107 ± 0.012,0.090 ± 0.010,F = 64.297,P < 0.001;12 wk:0.140 ± 0.007 vs 0.120 ± 0.010,0.097 ± 0.015,F = 16.392,P < 0.001);PDX-1 protein in RYGB group was also increased significantly(1 wk:0.61 ± 0.01 vs 0.21 ± 0.01,0.15 ± 0.01,F = 3031.127,P < 0.001;2 wk:0.55 ± 0.00 vs 0.15 ± 0.01,0.17 ± 0.01,F = 3426.455,P < 0.001;4 wk:0.39 ± 0.01 vs 0.18 ± 0.01,0.22 ± 0.01,F = 882.909,P < 0.001;12 wk:0.41 ± 0.01 vs 0.20 ± 0.01,0.18 ± 0.01,F = 515.833,P < 0.001).PDX-1 mRNA and PDX-1 protein production showed no statistical significance between the two sham groups.Many PDX-1 positive cells could be found in the pancreatic islets of the rats in RYGB group at all time points.In addition,the percentage of Brdu-insulin double staining positive cells was higher in RYGB group than in the other two groups(1 wk:0.22 ± 0.13 vs 0.03 ± 0.06,0.03 ± 0.06,P < 0.05;2 wk:0.28 ± 0.08 vs 0.00 ± 0.00,0.03 ± 0.06,P < 0.05;4 wk:0.24 ± 0.11 vs 0.07 ± 0.06,0.00 ± 0.00,P < 0.001;12 wk:0.20 ± 0.07 vs 0.03 ± 0.06,0.00 ± 0.00,P < 0.05).CONCLUSION:RYGB can increase the expression of pancreatic PDX-1 and induce the regeneration of β-cells in GK rats.The associated regeneration of islet cells may be a possible mechanism that how RYGB could improve type 2 diabetes mellitus. | Zhen Li,Dong-Fei Li,Jing-Xing Dai,Yu Bai,Lin Yuan,Southern Medical University,Institute of Basic Medical Anatomy National Key Disciplines,Guangzhou 510515,Guangdong Province,China Hong-Ya Zhang,Lu-Xian Lv,Wen-Qiang Li,Second Affiliated Hospital,Xinxiang Medical College,Henan Province Key Laboratory of Biological Psychiatry,Xinxiang 453002,Henan Province,China Ou Sha,Department of Anatomy,Faculty of Medicine,Shenzhen University,Shenzhen 518060,Guangdong Province,China | 2010 | World Journal of Gastroenterology2010，16，18： | 14 |
| 20 | Gastrin,somatostatin,and experimental disturbance of the gastrointestinal tract in rats显示文摘INTRODUCTIONThe field of gastrointestinal hormones has expanded at a dizzying rate[1-4].Gastrointestinal hormones as regulatory peptides that appear to be major components of bodily integration and have important regulatory actions on physioligical function of the gastrointestinal tract .The successful isolation of some gastrointestinal hormones and the development of sensitive methods for their detection have led to the unexpected finding that they also exist in the brain . | Yong Li Yao~1 Bo Xu~2 Wan Dai Zhang~1 Yu Gang Song~1 ~1Institute of Gastrointestinal Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2Department of Orthopedics Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaYong Li Yao graduated from First Military Mcdical University with a master degree in 1998.She now works in the Institute of Gastrointestinal Diseases,Nanfang Hospital as a doctoral candidate majoring gastrointestinal diseases and gastrointestinal hormones,she has published 8 papers. | 2001 | World Journal of Gastroenterology2001，7，3： | 12 |
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