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| 1 | Pyroptosis is driven by non-selective gasdermin-D pore and its morphology is different from MLKL channel-mediated necroptosis显示文摘Necroptosis 和 pyroptosis 是有血浆膜破裂的一个普通特征的规划房间死亡的二种形式。这里,我们与 necroptosis 比较学习了 pyroptosis 的形态学和机制。与 necroptosis 不同, pyroptosis 经历膜 blebbing 并且生产 apoptotic 在血浆膜以前的像身体的房间伸出(称为的 pyroptotic 身体) 破裂。在 necroptosis 的破裂是像爆炸的,而在 pyroptosis 它导致房间变平。necroptosis 的实行被混合的系 kinase 调停,这被知道像域(MLKL ) 在血浆膜的 oligomers,而 gasdermin-D (GSDMD ) 调停在它由 caspase-1 或 caspase-11 的劈开以后的 pyroptosis。我们显示出那 N 终端碎片 caspase 劈开产生的 GSDMD (GSDMD-N ) 也形成 oligomer 并且移居到血浆膜杀死房间。MLKL 和 GSDMD-N 是脂性的,两蛋白质的 N 终端序列为他们的 oligomerization 和血浆膜 translocation 是重要的。不同于在血浆膜上形成隧道的 MLKL,那导致渗透地胀大的选择离子的流入冲破的房间, GSDMD-N 形成非选择的毛孔并且不依靠增加的 osmolarity 破坏房间。我们的学习表明 GSDMD 的形成毛孔的活动和 MLKL 的形成隧道的活动在 pyroptosis 和 necroptosis 决定血浆膜破裂的不同方法。 | Xin Chen Wan-ting He Lichen Hu Jingxian Li Yuan Fang Xin Wang Xiaozheng Xu Zhuo Wang Kai Huang Jiahuai Han | 2016 | Cell Research2016，26，9： | 110 |
| 2 | Photodynamic therapy-triggered on-demand drug release from ROS-responsive core-cross-linked micelles toward synergistic anti-cancer treatment显示文摘Polymeric micelles have demonstrated wide utility for chemodrug delivery,which however,still suffer from shortcomings such as undesired drug loading,disassembly upon dilution,pre-leakage of drug cargoes during systemic circulation,and lack of cancer-selective drug release.Herein,a poly(ethylene glycol)(PEG)-polyphosphoester-based,reactive oxygen species (ROS)-responsive,core-cross-linked (CCL) micellar system was developed to encapsulate both chemodrug (doxorubicin,Dox) and photosensitizer (chlorin e6,Ce6).The hydrophobic core of the micelles was cross-linked via a thioketal (TK)-containing linker,which notably enhanced the drug loading and micelle stability.In tumor cells,far-red light irradiation of Ce6 generated ROS to cleave the TK linkers and disrupt the micelle cores.As such,micelles were destabilized and Dox release was promoted,which thereafter imparted synergistic anti-cancer effect with ROS-mediated photodynamic therapy.This study provides an effective approach to realize the precise control over drug loading,formulation stability,and cancer-selective drug release using polymeric micelles,and would render promising utilities for the programmed anti-cancer combination therapy. | Yongjuan Li Jian Hu Xun Liu Yong Liu Shixian Lv Juanjuan Dang Yong Ji Jinlin He Lichen Yin | 2019 | Nano Research2019，12，5： | 5 |
| 3 | Preparation and analysis of immunocompetence of recombinant fusion protein of the immunodominant region in chlamydial protease-like activity factor from Chlamydophila pneumoniae and its application in serodiagnosis显示文摘To clone the gene coding the immunodominant region in the chlamydial protease-like activity factor(CPAF)from Chlamydophila pneumoniae,to analyze immunocompetence of the expressed protein, and to evaluate its value in serodiagnosis,the CPAF immunodominant region gene was amplified,ligated into a pGEX6p-2 vector,and then the expressed recombinant protein was purified with glutathione S- transferase(GST)agarose gel FF after renaturation,then identified by SDS-PAGE and Western blot.A new indirect ELISA was developed with the purified protein as coating antigen.The immunogenicity of the recombinant protein was evaluated by immunization to New Zealand rabbits,and its immunoreactivity was analyzed by reacting with anti-C,pneumoniae antibody.300 clinical sera samples were respectively de- tected by microimmunofluorescence(MIF)as reference method and the indirect ELISA,and the differ- ence between the two methods was analyzed.Cross-reactivity against Chlamydia trachomatis was investi- gated with the indirect ELISA to detect anti-C,trachomatis positive antisera.The results indicated that a 51.3 kDa recombinant protein was obtained.Western blot assay proved that the recombinant protein could merely specifically react with human anti-C.pneumoniae antisera.The titers of the specific IgG an- tibodies in the immunized New Zealand rabbits were above 1:16 000.Anti-C.pneumoniae IgG positive and negative reference sere were detected with the indirect ELISA,and the concordance rate of negative and positive results were both 100%(40/40).The sensitivity and specificity of the indirect ELISA in comparison with MIF were 93.8%(45/48)and 100%(252/252)separately by detecting 300 clinical sera samples,and the concordance rate between the two methods was 99.0%.No cross reaction against C.trachomatis was found with the indirect ELISA to detect anti-C,trachomatis positive antisera.In con- clusion,the prepared recombinant protein of the CPAF immunodominant region shows excellent immuno- competence and can be used to develop a new indirect ELISA as a method to detect anti-C.pneumoniae antibody for diagnosis of C.pneumoniae infection. | JIANG HUA ZHENG YI MOU WU TAO DING LI LICHEN JIA QIANG LIU SHUANG QUAN LIU | 2007 | Journal of Microbiology and Immunology2007，5，2： | 2 |
| 4 | Synthesis of hypoallergenic derivatives of the major allergen Fag t 1 from tartary buckwheat via sequence restructuring显示文摘 | YANG Zhenhuang LI Yuying LIChen | 2012 | Food and Chemical Toxocology2012，50，8： | 1 |
| 5 | Synthesis of sandwich-like TiO 2 @C composite hollow spheres with high rate capability and stability for lithium-ion batteries显示文摘 | Lichen Liu Qi Fan Chuanzhi Sun Xianrui Gu Hao Li Fei Gao Yanfeng Chen Lin Dong | 2013 | Journal of Power Sources2013，，： | 1 |
| 6 | A Multi-Agent Based Equipment Bidding System on the Web显示文摘This paper puts forward a new framework of multi-agent based equipment bidding system on the Web(MAEBSW). The purpose of the paper is to make the process of enterprise equipment bidding, especially the decisionprocess of enterprise equipment bidding, more intelligent. The system discussed in this paper is an efficient method forthe enterprises doing business electronically. We also present our opinion of the approaches to defining agents. We em-phasize the importance of agent being able to provide explanation about its behavior for the user or other agents. Based onthis opinion we design a new common architecture of most agents in MAEBSW. | Hu Lichen, Liu Li, Dong Yu & Ding Yuzhan(University of Science and Technology, Beijing 100083, P. R. China China North Vehicle Institute, Beijing 100072, P. R. China) | 2002 | Journal of Systems Engineering and Electronics2002，13，4： | 1 |
| 7 | A formal two-phase method for decomposition of complex design problems 显示文摘 | LIChen DING Zhendong LI Simon | 2005 | Journal of Mechanical Design2005，127，3： | 1 |
| 8 | Exposure assessment of phthalates in non-occupational populations in China显示文摘 | LICHEN ZHAO YAN LI LUXI | 2012 | Science of the Total Environment2012，，427428： | 1 |
| 9 | Exposure assessment of phthalates in non -occupational populations in China 显示文摘 | LIChen ZHAO Yah LI Luxi | 2012 | Sci Total Environ2012，428，： | 1 |
| 10 | Volcanic Debris-avalanche Deposits of the Laoheishan Volcano and Huoshaoshan Volcano in Wudalianchi显示文摘Large amounts of volcanic debris-avalanche deposits, which take the shape of hummocks, are distributed around the peripheries of the Laoheishan volcano and Huoshaoshan volcano in Wudalianchi World Geopark. In earlier times, they were called 'satellite volcanoes', namely, freestanding volcanoes. This paper points out that these deposits actually came from the collapse of the cones of these two volcanoes. When the lava flow spilled out at the base of the slope of the cones, the slope broke up and collapsed under the action of gravity. Later, ravines were formed on the slope. Caved slope clastics, accompanying lava flow, accumulated at the rims of the volcano cones. Although some accumulations may form very large cones, they are not volcanoes, but deposits of volcanic debris avalanches. | Chen Hongzhou Yang Jinshan Li Tianxiang Wei Qinghai Zhang Lichen Guo Deming | 2009 | Earthquake Research in China2009，23，3： | 0 |
| 11 | Evaluation of GOFP over four forest plots using RAMI and UAV measurements显示文摘Comparison and validation of canopy reflectance(CR)models are two important steps to ensure their reliability.Pure forest plantations are an ideal type of forest for validating CR models because of their simple background and the low variance in the crown structures which are usually assumed to be identical in most CR models.A Geometric Optical Model for Forest Plantations(GOFP)was compared using dataset in two radiation transfer model intercomparison exercise(RAMI)stands and validated using in situ dataset of detailed optical and structural data of two forest plantations in the Saihanba Forestry Center,China.The results show that(1)the tree distributions in stands described by the hypergeometric model in GOFP show good consistencies with the dataset in the two RAMI stands and measurements from the two Saihanba forest stands;and(2)the CRs simulated with GOFP are also compared well in the two RAMI stands and validated with measurements collected with unmanned aerial vehicles in the two Saihanba stands.GOFP shows a better consistency with the CR measurements than those from CR models for natual forestsbecause the tree distribution in forest plantations is described more reasonably in GOFP. | Jun Geng Qian Zhang Feng Qiu J.M.Chen Yongguang Zhang Weiliang Fan Lili Tu Jianwei Huang Shaoteng Wang Lichen Xu Jinchao Li | 2021 | International Journal of Digital Earth2021，14，10： | 0 |
| 12 | Total pelvic exenteration and a new model of diversion for giant primitive neuroectodermal tumor of prostate: A case report and review of the literature显示文摘The present study reports a rare primitive neuroectodermal tumor (PNET) of prostate.A 27-year-old male was admitted to Harbin Medical University Cancer Hospital (Harbin,China) for dysuria and dyschezia. Magnetic resonance imaging (MRI) revealed a large mass thatmay involve the bladder and rectum next to the prostate. Histopathological analysis of biopsyof prostate indicated mesenchymal origin tumor, and immunohistochemistric stainingconfirmed diagnosis of PNET of prostate. En bloc total pelvic exenteration and double barrelsigmoidostomy were performed. Double stomas in the skin incision were used for fecal andurinary diversion, respectively. Short-term outcome is satisfactory, while long-term efficacyremains to be poor. Clinical features of PNET of prostate should be paid much more attentionand radical surgery and adjuvant chemotherapy should be recommended. | Lichen Teng Liangjun Wei Li Li Yongpeng Xu Yongsheng Chen Yan Cao Wentao Wang Changfu Li | 2020 | Asian Journal of Urology2020，7，2： | 0 |
| 13 | Dependence of Plastic Stability on 3D Interface Layer in Nanolaminated Materials显示文摘The nanolaminated materials generally exhibit poor plasticity due to the fast onset of shear instability.Engineering interface structure is an eff ective approach for enhancing plasticity via postponing or suppressing the shear instability.Here,we introduce 4 nm thick CuNb 3D amorphous interface layers and Nb 3D crystalline interface layers in Cu nanolaminated materials,respectively.In situ micro-pillar compression tests show that samples with crystalline interface layers exhibit shear instability,while the samples with amorphous interface layers display uniform deformation.Since the plastic deformation of the singlecrystal crystalline interface layer is anisotropic,except for well-aligned slip systems,dislocations on other slip systems have a poor ability to transmit the 3D crystalline interface layer,leading to localized dislocations pileups and shear instability.In contrast,the amorphous interface layer which is plastically isotropic accommodates dislocations from arbitrary slip systems of the matrix,which can alleviate the stress concentrations at the interface,and thus suppresses the shear instability. | Shuimiao Jiang Lichen Bai Qi An Zhe Yan Weiming Li Kaisheng Ming Shijian Zheng | 2022 | Acta Metallurgica Sinica(English Letters)2022，35，11： | 0 |
| 14 | Optimization-Based Fragmental Transmission Method for Video Data in Opportunistic Networks显示文摘Multimedia data have become popularly transmitted content in opportunistic networks. A large amount of video data easily leads to a low delivery ratio. Breaking up these big data into small pieces or fragments is a reasonable option. The size of the fragments is critical to transmission efficiency and should be adaptable to the communication capability of a network. We propose a novel communication capacity calculation model of opportunistic network based on the classical random direction mobile model, define the restrain facts model of overhead, and present an optimal fragment size algorithm. We also design and evaluate the methods and algorithms with video data fragments disseminated in a simulated environment. Experiment results verified the effectiveness of the network capability and the optimal fragment methods. | Peng Li Xiaoming Wang Junling Lu Lichen Zhang Zaobo He | 2017 | Tsinghua Science and Technology2017，22，4： | 0 |
| 15 | Effect of CYP3A5 Polymorphism on Tacrolimus Concentration during Early Period after Kidney Transplantation in Recipients显示文摘 | Lichen Teng changxi Wang Lizhong Chen Jiali Li Jiguang Fei Suxiong Deng Jiang Qiu Jun Li Guodong Chen | 2009 | 器官移植内科学杂志2009，4，2： | 0 |
| 16 | Preparation and characterization of core-shell Al@PFHP with improving the combustion and ignition properties of aluminum powder显示文摘The dense alumina shell on the surface of aluminum powder will hinder the combustion of aluminum powder and increase its ignition temperature.In this study,the aluminum oxide shell layer on the surface of aluminum powder was removed with hydrofluoric acid by one-pot method,and 3-Perfluorohexyl-1,2-epoxypropane(PFHP)(F3C(CF2)5CH2C2H3O)was coated to form a uniform and controllable core-shell Al@PFHP.The core-shell AI@PFHP showed better thermal reaction and ignition performance.The exothermic enthalpy of Al@0.15 PFHP was increased by about 1.9 times,with lower ignition temperature(reduced by about 140℃)and longer burning duration(increased by about 1.5 times)after coating with PFHP,compared with raw aluminum powder.In addition,the formation of PFHP coating shell can effectively improve the hydrophobicity and corrosion resistance of aluminum powder. | Lichen Zhang Shuo Wang Xing Su Xiaodong Li Meishuai Zou | 2023 | Particuology2023，，6： | 0 |
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