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| 1 | The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance显示文摘DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance. | Pengxiang Chen Mingjia Yan Lei Li Jieqiang He Shuangxi Zhou Zhongxing Li Chundong Niu Chana Bao Fang Zhi Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 7 |
| 2 | Functional assembly of root-associated microbial consortia improves nutrient efficiency and yield in soybean显示文摘Root-associated microbes are critical for plant growth and nutrient acquisition. However, scant information exists on optimizing communities of beneficial root-associated microbes or the mechanisms underlying their interactions with host plants. In this report, we demonstrate that rootassociated microbes are critical influencers of host plant growth and nutrient acquisition. Three synthetic communities(SynComs) were constructed based on functional screening of 1,893 microbial strains isolated from root-associated compartments of soybean plants. Functional assemblage of SynComs promoted significant plant growth and nutrient acquisition under both N/P nutrient deficiency and sufficiency conditions.Field trials further revealed that application of SynComs stably and significantly promoted plant growth, facilitated N and P acquisition, and subsequently increased soybean yield. Among the tested communities, SynCom1 exhibited the greatest promotion effect, with yield increases of up to 36.1% observed in two field sites. Further RNA-seq implied that SynCom application systemically regulates N and P signaling networks at the transcriptional level, which leads to increased representation of important growth pathways, especially those related to auxin responses. Overall,this study details a promising strategy for constructing SynComs based on functional screening,which are capable of enhancing nutrient acquisition and crop yield through the activities of beneficial root-associated microbes. | Cunhu Wang Yanjun Li Mingjia Li Kefei Zhang Wenjing Ma Lei Zheng Hanyu Xu Baofeng Cui Ran Liu Yongqing Yang Yongjia Zhong Hong Liao | 2021 | Journal of Integrative Plant Biology2021，63，6： | 5 |
| 3 | Modeling of microstructure evolution of AZ80 magnesium alloy during hot working process using a unified internal state variable method显示文摘In this paper, a unified internal state variable(ISV) model for predicting microstructure evolution during hot working process of AZ80 magnesium alloy was developed. A novel aspect of the proposed model is that the interactive effects of material hardening, recovery and dynamic recrystallization(DRX) on the characteristic deformation behavior were considered by incorporating the evolution laws of viscoplastic flow, dislocation activities, DRX nucleation and boundary migration in a coupled manner. The model parameters were calibrated based on the experimental data analysis and genetic algorithm(GA) based objective optimization. The predicted flow stress, DRX fraction and average grain size match well with experimental results. The proposed model was embedded in the finite element(FE) software DEFORM-3 D via user defined subroutine to simulate the hot compression and equal channel angular extrusion(ECAE) processes. The heterogeneous microstructure distributions at different deformation zones and the dislocation density evolution with competitive deformation mechanisms were captured.This study can provide a theoretical solution for the hot working problems of magnesium alloy. | Zexing Su Chaoyang Sun Mingjia Wang Lingyun Qian Xintong Li | 2022 | Journal of Magnesium and Alloys2022，10，1： | 3 |
| 4 | A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.显示文摘Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned genotypes.However,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish genotypes.The R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome 2.The amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of radish.The overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural genes.RsPAP2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid assay.The promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and RsTT8.These findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish. | Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong Liwang Liu | 2020 | Horticulture Research2020，7，1： | 2 |
| 5 | Effects of Deformation and Phase Transformation Microstructures on Springback Behavior and Biocompatibility in β-Type Ti-15Mo Alloy显示文摘This study examined the mechanical properties, springback behavior from three-point bending loading–unloading tests and biocompatibility from human osteoblast cell adhesion and proliferation experiments in Ti-15Mo alloy with different microstructures. The springback ratio increased after the appearance of deformation microstructures including {332} < 113 > twins and dislocations, due to the increased bending strength and unchanged Young’s modulus. By contrast, the change in springback ratio was dependent on the competing effect of the simultaneous increase in bending strength and Young’s modulus after phase transformation, namely, the isothermal ω-phase formation. Good cell adhesion and proliferation were observed on the alloy surface, and they were not significantly affected by the deformation twins, dislocations and isothermal ω-phase.The diversity of deformation and phase transformation microstructures made it possible to control the springback behavior effectively while keeping the biocompatibility of the alloy as an implant rod used for spinal fixation devices. | Sujie Zhang Xiaohua Min Yada Li Weiqiang Wang Ping Li Mingjia Li | 2022 | Acta Metallurgica Sinica(English Letters)2022，35，4： | 2 |
| 6 | A review on artificial intelligence in high-speed rail显示文摘High-speed rail(HSR)has brought a number of social and economic benefits,such as shorter trip times for journeys of between one and five hours;safety,security,comfort and on-time commuting for passengers;energy saving and environmental protection;job creation;and encouraging sustainable use of renewable energy and land.The recent development in HSR has seen the pervasive applications of artificial intelligence(AI).This paper first briefly reviews the related disciplines in HSR where AI may play an important role,such as civil engineering,mechanical engineering,electrical engineering and signalling and control.Then,an overview of current AI techniques is presented in the context of smart planning,intelligent control and intelligent maintenance of HSR systems.Finally,a framework of future HSR systems where AI is expected to play a key role is provided. | Mingjia Yin Kang Li Xiaoqing Cheng | 2020 | Transportation Safety and Environment2020，2，4： | 2 |
| 7 | Genome-wide identification of droughtresponsive microRNAs in two sets of Malus from interspecific hybrid progenies显示文摘Drought stress can negatively impact apple fruit quality and yield.Apple microRNAs(miRNAs)participate in apple tree and fruit development,as well as in biotic stress tolerance;however,it is largely unknown whether these molecules are involved in the drought response.To identify drought-responsive miRNAs in Malus,we first examined the drought stress tolerance of ten F1 progenies of R3(M.×domestica)×M.sieversii.We performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive plants.The sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought conditions.We identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought conditions.Under drought stress,61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants,respectively.We determined the expression levels of seven out of eight miRNAs by stem-loop qPCR analysis.We also predicted the target genes of all differentially expressed miRNAs and identified the expression of some genes.Gene Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic processes.Finally,we confirmed roles of two miRNAs in apple response to mannitol.Our results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species,thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress. | Chundong Niu Haiyan Li Lijuan Jiang Mingjia Yan Cuiying Li Dali Geng Yinpeng Xie Yan Yan Xiaoxia Shen Pengxiang Chen Jun Dong Fengwang Ma Qingmei Guan | 2019 | Horticulture Research2019，6，1： | 2 |
| 8 | A reduced graphene oxide/Co3O4 composite for supercapacitor electrode显示文摘 | Xiang Chengcheng Li Ming Zhi Mingjia | 2013 | Journal of Power Source2013，226，： | 1 |
| 9 | Effect of rare emlh elements on the thermal eracking resistance of high speed steel rolls显示文摘 | Wang Mingjia Li Yanmei Wang Zixi | 2011 | Jou ''nal of rare earths2011，29，5： | 1 |
| 10 | Parametric optimiza-tion ofregenerative Organic Rankine Cycle (ORC) for lowgrade waste heat recovery using genetic algorithm显示文摘 | Huan Xi Mingjia Li Chao Xu | 2013 | En-ergy2013，58，： | 1 |
| 11 | Life Cycle Assessment Analysis and Comparison of 1000 MW S-CO_(2)Coal Fired Power Plant and 1000 MW USC Water-Steam Coal-Fired Power Plant显示文摘The objective of this paper is to understand the benefits that one can achieve for large-scale supercritical CO_(2)(S-CO_(2))coal-fired power plants.The aspects of energy environment and economy of 1000 MW S-CO_(2)coal-fired power generation system and 1000 MW ultra-supercritical(USC)water-steam Rankine cycle coal-fired power generation system are analyzed and compared at the similar main vapor parameters,by adopting the neural network genetic algorithm and life cycle assessment(LCA)methodology.Multi-objective optimization of the 1000 MW S-CO_(2)coal-fired power generation system is further carried out.The power generation efficiency,environmental impact load,and investment recovery period are adopted as the objective functions.The main vapor parameters of temperature and pressure are set as the decision variables.The results are concluded as follows.First,the total energy consumption of the S-CO_(2)coal-fired power generation system is 10.48 MJ/k Wh and the energy payback ratio is 34.37%.The performance is superior to the USC coal-fired power generation system.Second,the resource depletion index of the S-CO_(2)coal-fired power generation system is 4.38μPRchina,90,which is lower than that of the USC coal-fired power generation system,and the resource consumption is less.Third,the environmental impact load of the S-CO_(2)coal-fired power generation system is 0.742 m PEchina,90,which is less than that of the USC coal-fired power generation system,0.783 m PEchina,90.Among all environmental impact types,human toxicity potential HTP and global warming potential GWP account for the most environmental impact.Finally,the investment cost of the S-CO_(2)coal-fired power generation system is generally less than that of the USC coal-fired power generation system because the cost of the S-CO_(2)turbine is only half of the cost of the steam turbine.The optimal turbine inlet temperature T_(5)becomes smaller,and the optimal turbine inlet pressure is unchanged at 622.082°C/30 MPa. | LI Mingjia WANG Ge XU Jinliang NI Jingwei SUN Enhui | 2022 | Journal of Thermal Science2022，31，2： | 1 |
| 12 | Fluid-Thermal-Mechanical Coupled Analysis and Optimized Design of Printed Circuit Heat Exchanger with Airfoil Fins of S-CO_(2) Brayton Cycle显示文摘Printed Circuit Heat Exchanger(PCHE) with high-efficiency and compact structure has great application prospect in the supercritical carbon dioxide(S-CO_(2)) power systems for the next generation of high-temperature concentrated solar and advanced nuclear energy. However, the high operating temperature and pressure require PCHE to maintain good heat transfer performance, as well as reliable mechanical performance at the same time. It is necessary to carry out the fluid-thermal-mechanical coupled analysis of PCHE for the safe and efficient operation of the S-CO_(2) cycle. In this paper, a three-dimensional fluid-structure coupled numerical model was established to study the fluid-thermal-mechanical coupled characteristics of PCHE under different airfoil fin arrangements. The stress distribution of the single airfoil fin was studied, and a better airfoil arrangement that comprehensively considers heat transfer characteristics and stress distribution was obtained. Aiming at the high stress caused by the stress concentration at both ends of the airfoil fin, an optimized configuration combining straight channel and airfoil channel was proposed. The results show that the difference between the flow and heat transfer performance of the two optimized structures and the reference structure is only within 1.5%, but the maximum stresses of the two optimized structures are respectively reduced by 69.4% and 70.0% compared with that of the reference structure, which significantly reduces the stress intensity of PCHE. The result provides a new method to develop the airfoil PCHE with uniform stress distribution and good thermo-hydraulic performance. | JIANG Tao LI Mingjia WANG Wenqi LI Dong LIU Zhanbin | 2022 | Journal of Thermal Science2022，31，6： | 1 |
| 13 | A reduced graphene oxide/Co304 composite for supercapacitor electrode显示文摘 | Xiang Chengcheng Li Ming Zhi Mingjia | 2013 | J Power Sources2013，226，： | 1 |
| 14 | Generic amyloid fibrillation of TMEM106B in patient with Parkinson’s disease dementia and normal elders显示文摘Dear Editor,Protein amyloid aggregation is a histological hallmark of neurodegenerative diseases(NDs).1,2 In synucleinopathies including Parkinson’s disease(PD),Parkinson’s disease dementia(PDD),dementia with Lewy body and multiple system atrophy,αsynuclein(α-syn)is commonly characterized to form amyloid aggregates presenting distinct pathological activities in diseased brains.3,4 Structural characterization ofα-syn amyloid fibrils formed in different diseases could provide mechanistic understanding of heterogeneousα-syn pathologies. | Yun Fan Qinyue Zhao Wencheng Xia Youqi Tao Wenbo Yu Mingjia Chen Yiqi Liu Jue Zhao Yan Shen Yunpeng Sun Chenfang Si Shenqing Zhang Yaoyang Zhang Wensheng Li Cong Liu Jian Wang Dan Li | 2022 | Cell Research2022，32，6： | 1 |
| 15 | Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1显示文摘The primary cilium is a microtubule-based sensory organelle.The molecular mechanism that regulates ciliary dynamics remains elusive.Here,we report an unexpected finding that MLN4924,a small molecule inhibitor of NEDD8-activating enzyme(NAE),blocks primary ciliary formation by inhibiting synthesis/assembly and promoting disassembly.This is mainly mediated by MLN4924-induced phosphorylation of AKT1 at Ser473 under serum-starved,ciliary-promoting conditions.Indeed,pharmaceutical inhibition(by MK2206)or genetic depletion(via siRNA)of AKT1 rescues MLN4924 effect,indicating its causai role.Interestingly,pAKT 1-Ser473 activity regulates both ciliary synthesis/assembly and disassembly in a MLN4924 dependent manner,whereas pAKT-Thr308 determines the ciliary length in MLN4924-independent but VHL-dependent manner.Finally,MLN4924 inhibits mouse hair regrowth,a process requires ciliogenesis?Collectively,our study dem on strates an unexpected role of a neddylation inhibitor in regulation of ciliogenesis via AKT1,and pro?vides a proof-of-concept for potential utility of MLN4924 in the treatment of human diseases associated with abnormal ciliogenesis. | Hongmei Mao Zaiming Tang Hua Li Bo Sun Mingjia Tan Shaohua Fan Yuan Zhu Yi Sun | 2019 | Protein & Cell2019，10，10： | 1 |
| 16 | Photon-counting distributed free-space spectroscopy显示文摘Spectroscopy is a well-established nonintrusive tool that has played an important role in identifying and quantifying substances,from quantum descriptions to chemical and biomedical diagnostics.Challenges exist in accurate spectrum analysis in free space,which hinders us from understanding the composition of multiple gases and the chemical processes in the atmosphere.A photon-counting distributed free-space spectroscopy is proposed and demonstrated using lidar technique,incorporating a comb-referenced frequency-scanning laser and a superconducting nanowire single-photon detector.It is suitable for remote spectrum analysis with a range resolution over a wide band.As an example,a continuous field experiment is carried out over 72 h to obtain the spectra of carbon dioxide(CO_(2))and semi-heavy water(HDO,isotopic water vapor)in 6 km,with a range resolution of 60 m and a time resolution of 10 min.Compared to the methods that obtain only column-integrated spectra over kilometer-scale,the range resolution is improved by 2-3 orders of magnitude in this work.The CO_(2)and HDO concentrations are retrieved from the spectra acquired with uncertainties as low as±1.2%and±14.3%,respectively.This method holds much promise for increasing knowledge of atmospheric environment and chemistry researches,especially in terms of the evolution of complex molecular spectra in open areas. | Saifen Yu Zhen Zhang Haiyun Xia Xiankang Dou Tengfei Wu Yihua Hu Manyi Li Mingjia Shangguan Tianwen Wei Lijie Zhao Lu Wang Pu Jiang Chengjun Zhang Lixing You Leigang Tao Jiawei Qiu | 2021 | Light(Science & Applications)2021，10，11： | 1 |
| 17 | Lattice Boltzmann Method for Conduction and Radiation Heat Transfer in Composite Materials显示文摘In this work,the utilization of lattice Boltzmann method(LBM)in the simulation of coupled conduction and radiation heat transfer in composite materials is studied.The novel D3Q30-LBM and D3Q38-LBM models are proposed for the simulation of radiative transfer equation(RTE).The LBM-LBM model,coupled finite volume method(FVM)and LBM are compared with the coupled FVM and discrete ordinate method(DOM)for 2D and 3D simulations.The results show that the original D3Q26-LBM is insufficient for the simulation of radiation,and both the D3Q30-LBM and D3Q38-LBM are close to the DOM for the RTE.The LBM can have large errors in the simulation of heat conduction when the relaxation time is large.Thus,its application in the composite materials is limited when the ratio between thermal conductivities of different components is large.The models with LBM for RTE can be more efficient than the FVM-DOM for the simulation of conduction-radiation heat transfer in composite materials.The FVM-D3Q30-LBM model is suggested because of its accuracy and efficiency. | TONG Zixiang LI Mingjia XIE Tao GU Zhaolin | 2022 | Journal of Thermal Science2022，31，3： | 1 |
| 18 | Study of Graphdiyne-based Magnetic Materials显示文摘Carbon-based magnetic semiconductors are easy to be modified with low cost and low power consumption.While they can demonstrate robust long-range magnetic ordering and show great potential for application after introducing magnetic moments.Graphdiyne(GDY),as an allotrope of carbon,exhibits intrinsic semiconductor properties and paramagnetic properties due to its unique structure and the presence of sp carbon.To improve the magnetic properties of GDY and prepare excellent magnetic semiconductor materials,scientists have done a lot of related research work.The most direct and effective method to introduce magnetism is heteroatom doping.In this review,we have entirely described the latest GDY magnetism introduction methods,effects,and theoretical calculations,etc.Doping methods include post-doping and molecular design doping.The doping elements have covered non-metallic elements(N,H,F,Cl,S),metallic elements(Fe),and functional groups.The magnetic properties of the modified GDY materials were studied by experimental analysis and theoretical calculations.This review provides a sufficient basis and direction for related researches. | LI Ru ZHANG Mingjia LI Xiaodong MA Xiaodi HUANG Changshui | 2021 | Chemical Research in Chinese Universities2021，37，6： | 0 |
| 19 | High-throughput screening of room temperature active Peltier cooling materials in Heusler compounds显示文摘Active Peltier cooling enables Peltier heat transfer in addition to the traditional Fourier thermal conductance,which is useful in some special applications,such as the microthermostats.From the material wise,however,the study on the active Peltier cooling materials is rare.We carried out a high-throughput workflow to screen out 5 room-temperature active Peltier cooling materials,GaSbLi_(2),HgPbCa_(2),SnTiRu_(2),GeYbLi_(2),and GeTiFe_(2),from 2958 Heusler materials.All the five materials are semimetals or very narrow band gap systems with high electrical conductivity.Some of these materials have relatively large Seebeck coefficients due to the band asymmetry.Their effective thermal conductivityκ_(eff)s,which are the summation of active Peltier thermal conductivity and passive thermal conductivity,are all greater than Cu at the room temperature andΔT=1 K.The present work gives a possible way to search active cooling Peltier materials for the applications of precise temperature control. | Huifang Luo Xin Li Yuxiang Wang Yeqing Jin Mingjia Yao Jiong Yang | 2022 | npj Computational Materials2022，，1： | 0 |
| 20 | Melatonin-induced DNA demethylation of metal transporters and antioxidant genes alleviates lead stress in radish plants显示文摘Melatonin(MT)is a tryptophan-derived natural product that plays a vital role in plant response to abiotic stresses,including heavy metals(HMs).However,it remains elusive how exogenous MT mediates lead(Pb)accumulation and detoxification at the methylation and transcriptional levels in radish.In this study,decreased Pb accumulation and increased antioxidant enzyme activity were detected under MT treatment in radish.Single-base resolution maps of DNA methylation under Pb stress(Pb200)and Pb plus MT treatment(Pb_50MT)were first generated.The genome-wide methylation level was increased under Pb stress,while an overall loss of DNA methylation was observed under MT treatment.The differentially methylated region(DMR)-associated genes between Pb_50MT and Pb200 were uniquely enriched in ion binding terms,including cation binding,iron ion binding,and transition metal ion binding.Hyper-DMRs between Pb200 and Control exhibited a decreasing trend of methylation under Pb_50MT treatment.A few critical upregulated antioxidant genes(e.g.,RsAPX2,RsPOD52 and RsGST)exhibited decreased methylation levels under MT treatment,which enabled the radish plants to scavenge lead-induced reactive oxygen species(ROS)and decrease oxidative stress.Notably,several MT-induced HM transporter genes with low methylation(e.g.,RsABCF5,RsYSL7 and RsHMT)and transcription factors(e.g.,RsWRKY41 and RsMYB2)were involved in reducing Pb accumulation in radish roots.These findings could facilitate comprehensive elucidation of the molecular mechanism underlying MT-mediated Pb accumulation and detoxification in radish and other root vegetable crops. | Mingjia Tang Liang Xu Yan Wang Junhui Dong Xiaoli Zhang Kai Wang Jiali Ying Cui Li Liwang Liu | 2021 | Horticulture Research2021，8，1： | 0 |
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