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| 1 | Effect of Nimesulide on proliferation and apoptosis of human hepatoma SMMC-7721 cells显示文摘AIM: Cyclooxygenase-2 (COX-2) has been suggested to beassociated with carcinogenesis. We sought to investigatethe effect of the selective COX-2 inhibitor, Nimesulide onproliferation and apoptosis of SMMC-7721 human hepatomacells.METHODS: This study was carried out on the culture ofhepatic carcinoma SMMC-7721 cell line. Variousconcentrations of Nimesulide (0、200 μmol/L、300 μmol/L、400μmol/L) were added and incubated. Cell proliferation wasdetected with MTT colorimetric assay, cell apoptosis byelectron microscopy, flow cytometry and TUNEL.RESULTS: Nimesulide could significantly inhibit SMMC-7721cells proliferation dose-dependent and in a dependentmanner compared with that of the control group. Theduration lowest inhibition rate produced by Nimesulide inSMMC-7721 cells was 19.06 %, the highest inhibition ratewas 58.49 %. After incubation with Nimesulide for 72 h, themost highest apoptosis rate and apoptosis index of SMMC-7721 cells comparing with those of the control were 21. 20 %+1.62% vs2.24% +0.26% and21.23+ 1.78 vs2.01+0.23(p<0.05).CONCLUSION: The selective COX-2 inhibitor, Nimesulide caninhibit the proliferation of SMMC-7721 cells and increaseapoptssis rate and apoptosis index of SMMC-7721 cells. Theapoptoois rate and the apoptosis index are dose-dependent.Under electron microscope SMMC-7721 cells incubated with 300μmol and 400 μmol Nimesulide show apoptotic characteristics.With the clarification of the mechanism of selective COX-2inhibitors, These COX-2 selective inhibitors can become thechoice of prevention and treatment of cancers. | Geng Tian Jie-Ping Yu He-Sheng Luo Bao-Ping Yu Hui Yue Jian-Ying Li Oiao Mei,Gastroenterology department,Renmin hospital of Wuhan university,Wuhan 430060,Hubei Province,China | 2002 | World Journal of Gastroenterology2002，8，3： | 51 |
| 2 | Expression of TFF2 and Helicobacter pylori infection in carcinogenesis of gastric mucosa显示文摘AIM: To investigate the expression of TFF2 and Helicobacter pyloriinfection in carcinogenesis of gastric mucosa.METHODS: The expression of TFF2 was immunohistochemically analyzed in paraffin-embedded samples from 119 patients with endoscopic biopsy and subtotal gastrectomy specimens of gastric mucosal lesions, including 16 cases of chronic superficial gastritis (CSG), 20 chronic atrophic gastritis (CAG),35 intestinal metaplasia (IN), 23 gastric epithelial dysplasia (GED) and 25 gastric carcinoma (CA), and Helicobacter pylori infection was detected by Warthin-Starry staining.RESULTS: 1:TFF2 was located in the cytoplasm of gastrk mucous neck cell. The expression of TFF2 was 100 %,100 %, 0, 56.5 % and 0 in CSGs, CAGs, INs, GEDs and CAs, respectively. 2: The value of TFF2 positive cell density in CSG with Helicobacter pyloriinfection was higher than that without Helicobacter pyloriinfection. (52.89±7.27vs46.49±13.04, P>0.05); But the value of TFF2 positive cell density in CAG and GED with Helicobacter pyloriinfection was significantly lower than that without Helicobacter pylori infection (18.17±4.09 vs 37.93±13.80, P<0.01 and 14.44±9.32 vs 24.84±10.22, P<0.05).CONCLUSION: Increase of TFF2 expression in CSG is perhaps associated with the protective mechanism after gastric mucosal injury. Decrease of TFF2 expression in CAG possibly attributes to the decrease in the number of gastric gland cell expressing TFF2. Re-expression of TFF2 in gastric epithelial dysplasia implies that TFF2 possibly contributes to the initiation of gastric carcinoma. The effect of Helicobacter pylori on the expression of TFF2 depends on the status of gastric mucosa. | Guo-Yong Hu Bao-Ping Yu Wei-Guo Dong Mu-Qi Li Jie-Ping Yu He-Sheng Luo Zong-Xue Rang Gastroenterology Department,Renmin Hospital of Wuhan University,Wuhan 430060,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 10 |
| 3 | Preventive effect of hydrotalcite on gastric mucosal injury in rats induced by taurocholate显示文摘AIM: To study the preventive effect of hydrotalcite on gastric mucosal injury in rat induced by taurocholate, and to investigate the relationship between the protective mechanism of hydrotalcite and the expression of trefoil factor family 2 (TFF2) mRNA and c-fos protein.METHODS: Forty five male Wistar rats were randomly divided into hydrotalcite group, ranitidine group and control group. Gastric mucosal injury was induced by introgastric acidified taurocholate. OD value of TFF2 mRNA expression in gastric mucous cells was determined by hybridization and computer image analysis system. OD value of c-fos protein expression in gastric mucous cells was measured by immunohistochemistry and computer image analysis system.RESULTS: The gross mucosal injury index in hydrotalcite group was significantly lower than that in ranitidine group and control group (8.60±2.20 vs 16.32±4.27, 29.53±5.39;P<0.05, P<0.01). The expression level of TFF2 mRNA in hydrotalcite group was markedly higher than that in ranitidine group and control group (0.56±0.09 vs 0.30±0.05, 0.28±0.03,P<0.05). The OD value of c-fos protein in hydrotalcite group was higher than that in ranitidine group and control group (0.52±0.07 vs 0.31±0.04, 0.32±0.05, P<0.05).CONCLUSION: Hydrotalcite can protect gastric mucosal injury in rats induced by taurocholate, which may be related to the increased expression of TFF2 and c-fos protein. | Bao-Ping Yu Jun Sun Mu-Qi Li He-Sheng Luo Jie-Ping Yu Department of Gastroenterology,Renmin Hospital of Wuhan University Wuhan 430060 Huhei Province China. | 2003 | World Journal of Gastroenterology2003，9，7： | 8 |
| 4 | Tea polyphenols inhibit expressions of iNOS and TNF-a and prevent lipopolysaccharide-induced liver injury in rats显示文摘BACKGROUND: Tea polyphenols have been shown to protect against carbon tetrachloride ( CCl4) -induced liver injury, liver fibrosis, hepatic ischemia-reperfusion injury. In this study, we examined the effect of tea polyphenols on lipopolysaccharide ( LPS ) -induced liver injury, and explored its mechanisms. METHODS: Sprague-Dawley rats received tea polyphenols (100 mg · kg-1·d-1) or vehicle (water) intragastrically by gavage for 14 days, followed by LPS (5 mg/kg) or saline injection intraperitoneally. Liver injury was assessed by biochemical assay and pathological analysis. Serum tumor necrosis factor-α (TNF-α) levels and liver malondialdehyde (MOA) contents were determined. Inducible nitric oxide synthase (iNOS) protein and TNF-α, iNOS and en-dothelial nitric oxide synthase (eNOS) mRNA expressions in the liver were detected by immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR), respectively. RESULTS: Administration of LPS resulted in liver injury in rats, evidenced by elevated activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), hepatocellular necrosis, and neutrophil infiltration in the liver. These responses were associated with increased serum TNF-α levels, induced iNOS protein, expressions of TNF-α, iNOS mRNA in the liver and elevated lipid peroxidation at 90 minutes or 6 hours after LPS injection. Pretreatment with tea polyphenols attenuated LPS-induced liver injury, and blunted the rises of serum TNF-α levels and lipid peroxidation and the induction of expressions of TNF-α, iNOS in the liver. CONCLUSION: Tea polyphenols prevent LPS-induced liver injury, and the mechanisms may involve the reduction of serum TNF-α levels and lipid peroxidation and the suppression of TNF-α, iNOS expressions in the liver. | Guang-Jin Yuan, Zuo-Jiong Gong, Xiao-Mei Sun, Shi-Hua Zheng and Xi Li Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 5 |
| 5 | Vertical distribution and storage of soil organic and inorganic carbon in a typical inland river basin,Northwest China显示文摘Knowledge of soil carbon(C) distribution and its relationship with the environment can improve our understanding of its biogeochemical cycling and help to establish sound regional models of C cycling. However, such knowledge is limited in environments with complex landscape configurations. In this study, we investigated the vertical distribution and storage of soil organic carbon(SOC) and soil inorganic carbon(SIC) in the 10 representative landscapes(alpine meadow, subalpine shrub and meadow, mountain grassland, mountain forest, typical steppe, desert steppe, Hexi Corridor oases cropland, Ruoshui River delta desert, Alxa Gobi desert, and sandy desert) with contrasting bioclimatic regimes in the Heihe River Basin, Northwest China. We also measured the 87 Sr/86 Sr ratio in soil carbonate to understand the sources of SIC because the ratio can be used as a proxy in calculating the contribution of pedogenic inorganic carbon(PIC) to total SIC. Our results showed that SOC contents generally decreased with increasing soil depth in all landscapes, while SIC contents exhibited more complicated variations along soil profiles in relation to pedogenic processes and parent materials at the various landscapes. There were significant differences of C stocks in the top meter among different landscapes, with SOC storage ranging from 0.82 kg C/m^2 in sandy desert to 50.48 kg C/m^2 in mountain forest and SIC storage ranging from 0.19 kg C/m^2 in alpine meadow to 21.91 kg C/m^2 in desert steppe. SIC contributed more than 75% of total C pool when SOC storage was lower than 10 kg C/m^2, and the proportion of PIC to SIC was greater than 70% as calculated from Sr isotopic ratio, suggesting the critical role of PIC in the C budget of this region. The considerable variations of SOC and SIC in different landscapes were attributed to different pedogenic environments resulted from contrasting climatic regimes, parent materials and vegetation types. This study provides an evidence for a general trade-off pattern between SOC and SIC, showing the compensatory effects of environmental conditions(especially climate) on SOC and SIC formation in these landscapes. This is largely attributed to the fact that the overall decrease in temperature and increase in precipitation from arid deserts to alpine mountains simultaneously facilitate the accumulation of SOC and depletion of SIC. | YANG Fan HUANG Laiming YANG Renmin YANG Fei LI Decheng ZHAO Yuguo YANG Jinling LIU Feng ZHANG Ganlin | 2018 | Journal of Arid Land2018，10，2： | 4 |
| 6 | Ruin probability of the renewal model with risky investment and large claims显示文摘The ruin probability of the renewal risk model with investment strategy for a capital market index is investigated in this paper.For claim sizes with common distribution of extended regular variation,we study the asymptotic behaviour of the ruin probability.As a corollary,we establish a simple asymptotic formula for the ruin probability for the case of Pareto-like claims. | WEI Li School of Finance,Renmin University of China,Beijing 100872,China | 2009 | Science China Mathematics2009，52，7： | 4 |
| 7 | Approximation for a scheduling problem with application in wireless networks显示文摘A network of many sensors and a base station that are deployed over a region is considered.Each sensor has a transmission range,an interference range and a carrier sensing range,which are r,αr and βr,respectively.In this paper,we study the minimum latency conflict-aware many-to-one data aggregation scheduling problem:Given locations of sensors along with a base station,a subset of all sensors,and parameters r,α and β,to find a schedule in which the data of each sensor in the subset can be transmitted to the base station with no conflicts,such that the latency is minimized.We designe an algorithm based on maximal independent sets,which has a latency bound of(a+19b)R + Δb-a + 5 time slots,where a and b are two constant integers relying on α and β,Δ is the maximum degree of network topology,and R is the trivial lower bound of latency.Here Δ contributes to an additive factor instead of a multiplicative one,thus our algorithm is nearly a constant(a+19b)-ratio. | ZHU QingHua & LI DeYing Key Laboratory of Data Engineering and Knowledge Engineering,MOE,School of Information,Renmin University of China,Beijing 100872,China | 2010 | Science China Mathematics2010，53，6： | 4 |
| 8 | Mapping Soil Texture Based on Field Soil Moisture Observations at a High Temporal Resolution in an Oasis Agricultural Area显示文摘Due to the almost homogeneous topography in low relief areas, it is usually difficult to make accurate predictions of soil properties using topographic covariates. In this study, we examined how time series of field soil moisture observations can be used to estimate soil texture in an oasis agricultural area with low relief in the semi-arid region of northwest China. Time series of field-observed soil moisture variations were recorded for 132 h beginning at the end of an irrigation event during which the surface soil was saturated.Spatial correlation between two time-adjacent soil moisture conditions was used to select the factors for fuzzy c-means clustering. In each of the ten generated clusters, soil texture of the soil sample with the maximum fuzzy membership value was taken as the cluster centroid. Finally, a linearly weighted average was used to predict soil texture from the centroids. The results showed that soil moisture increased with the increase of clay and silt contents, but decreased with the increase of sand content. The spatial patterns of soil moisture changed during the entire soil drying phase. We assumed that these changes were mainly caused by spatial heterogeneity of soil texture. A total of 64 independent samples were used to evaluate the prediction accuracy. The root mean square error(RMSE)values of clay, silt and sand were 1.63, 2.81 and 3.71, respectively. The mean relative error(RE) values were 9.57% for clay, 3.77% for silt and 12.83% for sand. It could be concluded that the method used in this study was effective for soil texture mapping in the low-relief oasis agricultural area and could be applicable in other similar irrigation agricultural areas. | YANG Renmin LIU Feng ZHANG Ganlin ZHAO Yuguo LI Decheng YANG Jinling YANG Fei YANG Fan | 2016 | Pedosphere2016，26，5： | 3 |
| 9 | Liver cold preservation induce lung surfactant changes and acute lung injury in rat liver transplantation显示文摘AIM:To investigate the relationship between donor liver cold preservation,lung surfactant (LS) changes and acute lung injury (ALI) after liver transplantation.METHODS:Liver transplantation models were estab-lished using male Wistar rats.Donor livers were pre-served in University of Wisconsin solution at 4 ℃ for different lengths of time.The effect of ammonium pyr-rolidinedithiocarbamate (PDTC) on ALI was also detect-ed.All samples were harvested after 3 h reperfusion.The severity of ALI was evaluated by lung weight/body weight ratio,lung histopathological score,serum nitric oxide (NO) and endothelin (ET)-1 levels,lung tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels.Lung surfactants (LSs) were determined by micellar electrokinetic capillary chromatography.RESULTS:With extended donor liver cold preservation time (CPT),lung histopathological scores,serum ET-1 levels,lung weight/body weight ratio and the level of TNF-α and IL-1β in lung were increased significantly in the 180-min group compared with the sham group (3.16 ± 0.28 vs 1.12 ± 0.21,P < 0.001;343.59 ± 53.97 vs 141.53 ± 48.48,P < 0.001;0.00687 ± 0.00037 vs 0.00557 ± 0.00056,P < 0.001;17.5 ± 3.0 vs 1.3 ± 0.3,P < 0.001;10.8 ± 2.3 vs 1.8 ± 0.4,P < 0.001),but se-rum NO levels decreased remarkably (74.67 ± 10.01 vs 24.97 ± 3.18,P < 0.001).The expression of lung phos-phatidylcholine (PC),phosphatidylethanolamine (PE),phosphatidylinositol (PI) and phosphatidylserine (PS) increased when CPT was < 120 min,and decreased when CPT was > 180 min (PC:1318.89 ± 54.79 vs 1011.18 ± 59.99,P < 0.001;PE:1504.45 ± 119.96 vs 1340.80 ± 76.39,P=0.0019;PI:201.23 ± 34.82 vs 185.88 ± 17.04,P=0.2265;PS:300.43 ± 32.95 vs 286.55 ± 55.55,P=0.5054).All these ALI-associated indexes could be partially reversed by PDTC treatment.CONCLUSION:Prolonged CPT could induce or inhibit the expression of LSs at the compensation or decom-pensation stage,and some antioxidants (e.g.,PDTC) may reverse the pathological process partially. | An Jiang,Chang Liu,Liang Yu,Yi Lv,Department of Hepatobiliary Surgery,the First Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710061,Shannxi Province,China An Jiang,Department of VIP Clinic,The Second Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710004,Shannxi Province,China Feng Liu,Department of Liver Transplantation,Qianfoshan Hospital,Jinan 250014,Shandong Province,China Yu-Long Song,Department of Anesthesiology,Renmin Hospital of Shannxi Province,Xi’an 710061,Shannxi Province,China Quan-Yuan Li,Department of Transplantation,Dongfeng Hospital,Shiyan 442008,Hubei Province,China | 2012 | World Journal of Gastroenterology2012，18，4： | 2 |
| 10 | Electrodif- fusive transport in charged porous media: from the parti- cle-level scale to the macroscopic scale using volume av- eraging显示文摘 | Pivonka P Narsilio G A Li Renmin | 2009 | Journal of Porous Media2009，12，2： | 1 |
| 11 | A quick iso- lation method for mutants with high lipid yield in oleagi- nous yeast 显示文摘 | WANG Jufang LI Renmin DONG Lu | 2009 | World J Microbiol Biotechnol2009，25，5： | 1 |
| 12 | Anion coordination complex [ClPt(bpt)_4]Cl (bpt= N,N'-bis(3-pyridylmethyl)-2-thiourea)显示文摘The anion coordination complex,[ClPt(bpt)4]Cl (bpt=N,N'-bis(3-pyridylmethyl)-2-thiourea),was synthesized and studied by X-ray crystal structure analysis,NMR and FAB mass spectra. In the solid state,the Pt(bpt)4 anion receptor adopts a cone conformation to bind the chloride anion through hydrogen bonds and electrostatic interaction in which the four branches of the thiourea ligands bind the chloride anion to form N-H…Cl-hydrogen bonds (3.49-3.81). The entraped chloride anion is situated above the Pt(Ⅱ) center at 3.52 . Further second-sphere coordination assemby from the Pt(bpt)4 core with 8 zinc(Ⅱ) tetraphenylporphyrins (ZnPr) is discussed. | LI Xin1,LI Hui2,YU ShuYan1,4 & LI YiZhi3 1 Laboratory for Self-Assembly Chemistry,Department of Chemistry,Renmin University of China,Beijing 100872,China 2 School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237,China 3 State Key Laboratory of Coordination Chemistry,Coordination Chemistry Institute,Nanjing University,Nanjing 210093,China 4 State Key Laboratory of Structural Chemistry,Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences,Fuzhou 350002,China | 2009 | Science China Chemistry2009，52，4： | 1 |
| 13 | Reparative isolation and purification of hydroxyanthraquinones and cinnamic acidfrom the Chinese medicinal herb Rheum officinale baill, by high-speed countercurrent chromatography 显示文摘 | Liu Renmin Li Aifeng Sun Ailing | 2004 | Journal of Chromatography A2004，1052，： | 1 |
| 14 | Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography显示文摘 | Renmin Liu Aifeng Li Ailing Sun Lingyi Kong | 2004 | Journal of Chromatography A2004，，1： | 1 |
| 15 | Fluid Origin and Structural Enhancement During Mineralization of the Jinshan Orogenic Gold Deposit, South China显示文摘 | Li Xiaofeng Wang Chunzeng Hua Renmin | 2010 | Mineralium Deposita2010，45，6： | 1 |
| 16 | Geochemical evolution and Metamorphic development of the Early Precambrian in Eastern Hebei,China显示文摘 | Wang Renmin Chen Zhenzhen Li Pingfan | 1985 | Precambrian Research1985，27，： | 1 |
| 17 | Removal of cationic dyes from aqueous solution by adsorption on peanut hull 显示文摘 | GONG Renmin LI Mei YANG Chao | 2005 | J Hazard Materi2005，121，： | 1 |
| 18 | Separation and Purifica- tion of Rutin and Acaciin from the Chinese Medicinal Herb Her- ba Cirsii by Combination of Macroporous Absorption Resin and High - Speed Counter - Current Chromatography 显示文摘 | Li Meng Renmin Liu Ailing Sun | 2009 | Journal of Chromatographic Science2009，47，： | 1 |
| 19 | Removal of cationic dyes from aqueous solution by adsorption on peanut hull显示文摘 | Renmin Gong Mei Li Chao Yang Yingzhi Sun Jian Chen | 2005 | Journal of Hazardous Materials2005，，1： | 1 |
| 20 | Defining hirsutism in Chinese women: a cross-sectional study显示文摘 | Xiaomiao Zhao Renmin Ni Lin Li Yaqin Mo Jia Huang Meifeng Huang Ricardo Azziz Dongzi Yang | 2011 | Fertility and Sterility2011，，3： | 1 |
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