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| 1 | Identification of Differentially Expressed Genes in Grape Skin at Veraison and Maturity and Construction of Co-expression Network显示文摘The ripening process of grape is an important stage during grape growth and development. During this process, color of grape skin is the most obvious change. The molecular mechanism for the ripening of grape(a non-climacteric fruit, which ripens without ethylene and respiration bursts) is still unclear. Although numerous studies have been done on the changes in the contents of metabolites during grape ripening, the differentially expressed genes at veraison and maturity stages have not been systematically analyzed. In this study, 1 524 genes that are significantly differentially expressed in grape(Pinot Noir) skin at veraison and maturity stages were identified, and a co-expression network of these genes was built. Some of the eight co-expression modules we identified may be closely related to the synthesis or metabolism of anthocyanins, sugar acids, and other flavor substances. The transcription factor families WRKY, b ZIP, HSF and WOX may play an important role in the regulation of anthocyanin synthesis or metabolism. The results provide a foundation for further study of the molecular mechanism of grape ripening. | Pengfei WANG Xilong JIANG Xinying WU Ling SU Lei GONG Hongmei SHI Fengshan REN Yongmei WANG | 2017 | Agricultural Science & Technology2017，18，11： | 2 |
| 2 | Organocatalytic cascade 1,6-conjugate addition/annulation/tautomerization of functionalized para-quinone methides: Access to chiral 2-amino-4-aryl-4H-chromenes显示文摘A novel organocatalytic cascade process initiated by 1,6-conjugated addition has been successfully developed. A range of pharmaceutically active 2-amino-4-aryl-4H-chromenes were readily obtained in high yields(88%-99%) and excellent enantiopurities(86%-99% ee). The functionalized para-Quinone methides(p-OMs) could be facilely obtained. | Cong Duan Ling Ye Wenqin Xu Xinying Li Feng Chen Zhigang Zhao Xuefeng Li | 2018 | Chinese Chemical Letters2018，29，8： | 0 |
| 3 | Advances in the Role of DNA Methylation and miRNAs in Plant Defense显示文摘DNA methylation and miRNAs are two important epigenetic molecular mechanisms in plants. RNA-directed DNA methylation( RdD M) pathway is considered as the most important mechanism of DNA methylation. A microRNA( miRNA) is processed from a 70-90-nucleotide-long single-stranded RNA precursor that forms a hairpin structure by Dicer enzyme,which plays an important role in plant development,regulation of plant gene expression and silencing of transposons. In recent years,a large number of studies have confirmed that DNA methylation and miRNAs play an important role in disease resistance mechanisms of plants. In this paper,recent progress on the role of DNA methylation and miRNAs in plant defense was summarized,which provided reference for revealing the relationship between disease resistance factors and epigenetic mechanisms in plants. | Pengfei WANG Xilong JIANG Xinying WU Ling SU Fengshan REN Yongmei WANG | 2017 | Agricultural Biotechnology2017，6，3： | 0 |
| 4 | Isotope implications of groundwater recharge,residence time and hydrogeochemical evolution of the Longdong Loess Basin,Northwest China显示文摘Groundwater plays a dominant role in the eco-environmental protection of arid and semi-arid regions.Understanding the sources and mechanisms of groundwater recharge,the interactions between groundwater and surface water and the hydrogeochemical evolution and transport processes of groundwater in the Longdong Loess Basin,Northwest China,is of importance for water resources management in this ecologically sensitive area.In this study,71 groundwater samples(mainly distributed at the Dongzhi Tableland and along the Malian River)and 8 surface water samples from the Malian River were collected,and analysis of the aquifer system and hydrological conditions,together with hydrogeochemical and isotopic techniques were used to investigate groundwater sources,residence time and their associated recharge processes.Results show that the middle and lower reaches of the Malian River receive water mainly from groundwater discharge on both sides of valley,while the source of the Malian River mainly comes from local precipitation.Groundwater of the Dongzhi Tableland is of a HCO3-Ca-Na type with low salinity.The reverse hydrogeochemical simulation suggests that the dissolution of carbonate minerals and cation exchange between Ca^(2+),Mg^(2+)and Na+are the main water-rock interactions in the groundwater system of the Dongzhi Tableland.Theδ^(18)O(from-11.70‰to-8.52‰)andδ2H(from-86.15‰to-65.75‰)values of groundwater are lower than the annual weighted average value of precipitation but closer to summer-autumn precipitation and soil water in the unsaturated zone,suggesting that possible recharge comes from the summer-autumn monsoonal heavy precipitation in the recent past(≤220 a).The corrected 14C ages of groundwater range from 3,000 to 25,000 a old,indicating that groundwater was mainly from precipitation during the humid and cold Late Pleistocene and Holocene periods.Groundwater flows deeper from the groundwater table and from the center to the east,south and west of the Dongzhi Tableland with estimated migration rate of 1.29-1.43 m/a.The oldest groundwater in the Quaternary Loess Aquifer in the Dongzhi Tableland is approximately 32,000 a old with poor renewability.Based on theδ^(18)O temperature indicator of groundwater,we speculate that temperature of the Last Glacial Maximum in the Longdong Loess Basin was 2.4℃-6.0℃ colder than the present.The results could provide us the valuable information on groundwater recharge and evolution under thick loess layer,which would be significative for the scientific water resources management in semi-arid regions. | LING Xinying MA Jinzhu CHEN Peiyuan LIU Changjie Juske HORITA | 2022 | Journal of Arid Land2022，14，1： | 0 |
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