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| 1 | Spatiotemporal microRNA profile in peripheral nerve regeneration:miR-138 targets vimentin and inhibits Schwann cell migration and proliferation显示文摘While the peripheral nervous system has regenerative ability,restoration of sufficient function remains a challenge.Vimentin has been shown to be localized in axonal growth fronts and associated with nerve regeneration,including myelination,neuroplasticity,kinase signaling in nerve axoplasm,and cell migration;however,the mechanisms regulating its expression within Schwann cell(SC) remain unexplored.The aim of this study was to profile the spatial and temporal expression profile of micro RNA(mi RNA) in a regenerating rat sciatic nerve after transection,and explore the potential role of mi R-138-5 p targeting vimentin in SC proliferation and migration.A rat sciatic nerve transection model,utilizing a polyethylene nerve guide,was used to investigate mi RNA expression at 7,14,30,60,and 90 days during nerve regeneration.Relative levels of mi RNA expression were determined using microarray analysis and subsequently validated with quantitative real-time polymerase chain reaction.In vitro assays were conducted with cultured Schwann cells transfected with mi RNA mimics and assessed for migratory and proliferative potential.The top seven dysregulated mi RNAs reported in this study have been implicated in cell migration elsewhere,and GO and KEGG analyses predicted activities essential to wound healing.Transfection of one of these,mi RNA-138-5 p,into SCs reduced cell migration and proliferation.mi R-138-5 p has been shown to directly target vimentin in cancer cells,and the luciferase assay performed here in rat Schwann cells confirmed it.These results detail a role of mi R-138-5 p in rat peripheral nerve regeneration and expand on reports of it as an important regulator in the peripheral nervous system. | Travis B.Sullivan Litchfield C.Robert Patrick A.Teebagy Shannon E.Morgan Evan W.Beatty Bryan J.Cicuto Peter K.Nowd Kimberly M.Rieger-Christ David J.Bryan | 2018 | Neural Regeneration Research2018，13，7： | 6 |
| 2 | 新西兰2016年凯库拉M_W7.8地震地表破裂带特征初析显示文摘新西兰2016年凯库拉M_W7.8地震地表破裂带分布在1个长约170km、宽35km的范围内,总体呈NE-SW走向,至少有12条断裂产生了m量级的地表位错,跨过了2个活动方式与活动强度存在明显差异的地震构造区。地震地表断裂大致可分为NE—NEE向和NNW—近SN向2组,NE—NEE向断裂之间的贯通性差,最大相隔距离为25~30km,即使首尾相连,走向上也有约30°的差异,运动性质以右旋走滑为主,最大位错量10~12m;NNW—近SN向断裂近于平行分布,相距可达40~50km,以逆断裂活动性质为主,最大垂直位错量5~6m。走滑类地表破裂带的组合特征非常复杂,主要表现为3种形式:雁列、分叉和平行分布。其中,雁列地表破裂(段)既可以表现为数m至数十m尺度上的张剪性破裂与鼓包、挤压剪切破裂组合,也可表现为百余m长的左阶斜列张剪性破裂组合;地表破裂段之间阶区规模差异明显,可以是数十m、数百m到数km不等。平行的地表破裂(段)可以相距数m、数十m至数km。凯库拉地震地表破裂带对已知活动断裂分布格局的突破也是1种比较显著的特点,既可以是在原先认为不活动的断裂上或没有活动断裂的位置上产生了地表破裂带,也可以是在走向或横向上突破了先前认识到的活动断裂分布范围。对凯库拉地震地表破裂带发育特征的初步分析结果,对于理解地表活动断裂与深部发震构造之间复杂的对应关系,以及跨活动断裂的抗震设防等问题具有一定的借鉴意义。 | 韩竹军 Nicola Litchfield 冉洪流 袁仁茂 郭鹏 Robert M Langridge Russ J Van Dissen | 2017 | 地震地质2017，39，4： | 6 |
| 3 | Randomised controlled trial of combined spinal epidural vs. spinal anaesthesia for elective caesarean section: vasopressor requirements and cardiovascular changes显示文摘 | Alan JR Macfarlane Artur Pryn Kerry N Litchfield Fiona Bryden Steven Young Christopher Weir Elizabeth M McGrady | 2009 | European Journal of Anaesthesiology2009，，1： | 2 |
| 4 | Relationship of the internal solute composition to the salt tolerance of Halomonas elongata 显示文摘 | Vreeland R H Mierau B D Litchfield C D | 1983 | Can J Microbiol1983，29，： | 1 |
| 5 | BMC Family Practice论文摘要汇编--全科医生对基层医疗机构中应用患者状况自评工具的看法:一项横断面调查与定性访谈分析显示文摘背景患者状况自评工具被日益广泛地用于评估疾病和治疗对患者生活质量和症状的影响。然而,其在基层医疗机构中的应用却尚未形成体系。本文旨在调查患者状况自评工具目前在基层医疗机构中的使用情况和影响其应用的有利和不利因素,以及如何优化其应用。方法本研究基于对英国全科医生进行的横断面调查和半结构化访谈。通过在线医生社区平台,向100例全科医生发放自填式电子调查问卷,问卷内容涉及患者状况自评工具在基层医疗机构中的应用情况、不利于其应用的因素以及能促进其有效利用的方法。另外对25例全科医生进行半结构化访谈并对结果进行定性分析。结果 77%(77/100)接受问卷调查的全科医生称其在诊疗过程中使用了至少一种患者状况自评工具,主要是用于辅助临床管理(n=66)或筛查/诊断(n=62)。全科医生在访谈中强调了其在鉴别和选择患者状况自评工具时遇到的挑战。不过部分全科医生认为,这些工具在医患共同决策以及指引患者谈论病情时有重要意义。访谈发现,全科医生认为应用这些工具的不利因素为:(1)诊疗过程中使用这些工具进行评估的时间有限。(2)相关应用知识不足。(3)工具未能和临床系统集成为一体。(4)政策层面鼓励应用这些工具,但是却未咨询医生或对医生做出解释,具有强制性质。如果要促进这些工具在基层医疗机构中的接受和应用程度,需要给出有益处的证据。结论在基层医疗机构中应用患者状况自评工具需要与临床系统集成为一体。在集成二者时,需要对患者和全科医生进行有意义的咨询以进行工具的选择和系统设计;在使用时,还要对其做好应用相关的培训/支持。 | TURNER G M LITCHFIELD I FINNIKIN S 本刊编辑部(编译) | 2020 | 中国全科医学2020，23，11： | 1 |
| 6 | Regula- tion of chemotactic networks by ' atypical' receptors 显示文摘 | Comerford I Litchfield W Harata-Lee Y | 2007 | BioEssays2007，29，3： | 1 |
| 7 | Mechanism of autoxidative glycosylation:identification of glyoxal and arabinose as intermediates in the autoxidative modification of proteins by glucose显示文摘 | Wells-Knecht KJ Zyzak DV Litchfield JE | 1995 | Biochemistry1995，34，11： | 1 |
| 8 | A re- view and comparative study of release coatings for optimised abhesion in resin transfer moulding applications显示文摘 | Critchlow G W Litchfield R E Sutherland I | 2006 | Int J Adhesion Adhesives2006，26，8： | 1 |
| 9 | THE TREATMENT OF UNSTABLE FRACTURES OF THE ANKLE IN THE ELDERLY显示文摘 | Litchfield J C | 1987 | INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED1987，18，2： | 1 |
| 10 | Functional specialization of CK2 isoforms and characterization of isoform-specific binding partners显示文摘 | Litchfield DW Bosc DG Canton DA | 2001 | Mol Cell Biochem2001，227，12： | 1 |
| 11 | Metabolites in safety testing assessment in early clinical development: a case study with a glucokinase activator 显示文摘 | Sharma R Litchfield J Atkinson K | 2014 | Drug Metab Dispos2014，42，11： | 1 |
| 12 | Tot} much of a good thing : the role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2显示文摘 | Duncan JS Litchfield DW | 2008 | Biochim Biophys Acta2008，1784，： | 1 |
| 13 | Partitioning soybean yield components 显示文摘 | Herbert S J Litchfield G V | 1982 | Crop Science1982，22，： | 1 |
| 14 | Computer image analysis for detection of maize and soybean kernel quality factors显示文摘 | Paulsen MR Wigger WD Litchfield JB | 1989 | Journal of Agricultural Engineering Research1989，43，2： | 1 |
| 15 | A randomized trialofarthroscopic surgery for osteoarthritis of the knee显示文摘 | Kirkley A Bim ingham TB Litchfield RB | 2008 | N Engl J Med2008，395，11： | 1 |
| 16 | Nuclear Magnetic Resonance Imaging of Transient 3-Dimensional Moisture Distribution in an Ear of Corn During Drying显示文摘 | Song HP Litchfield JB | 1990 | Cereal Chemistry1990，67，： | 1 |
| 17 | Discovery of( S)-6-( 3-cyclopentyl-2-( 4-( trifluoromethyl)-i H- imidazol-l-yl)propanamido)nicotinic acid as a hepatoselective glu- cokinase activator clinical candidate for treating type 2 diabetes mellitus显示文摘 | PFEFFERKORN J A GUZMAN-PEREZ A LITCHFIELD J | 2012 | Journal of Medicinal Chemistry2012，55，3： | 1 |
| 18 | Agricultural work related injury and Ill-health and the economic cost显示文摘 | Melville H. Litchfield | 1999 | Environmental Science and Pollution Research1999，，3： | 1 |
| 19 | Protein kinase CK2: structure, regulation and role in cellular decisions of life and death 显示文摘 | Litchfield DW | 2003 | Biochem J2003，369，1： | 1 |
| 20 | Physiologically based modeling of pravastatin transporter-mediated hepatobiliary disposition and drug-drug interactions 显示文摘 | Varma MV Lai Y Feng B Litchfield J Goosen TC Bergman A | 2012 | Pharm Res2012，29，10： | 1 |
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