| 1 | F-Actin reassembly during focal adhesion impacts single cell mechanics and nanoscale membrane structure显示文摘Focal adhesions play an important role in cell spreading,migration,and overall mechanical integrity.The relationship of cell structural and mechanical properties was investigated in the context of focal adhesion processes.Combined atomic force microscopy(AFM) and laser scanning confocal microscopy(LSCM) was utilized to measure single cell mechanics,in correlation with cellular morphology and membrane structures at a nanometer scale.Characteristic stages of focal adhesion were verified via confocal fluorescent studies,which confirmed three representative F-actin assemblies,actin dot,filaments network,and long and aligned fibrous bundles at cytoskeleton.Force-deformation profiles of living cells were measured at the single cell level,and displayed as a function of height deformation,relative height deformation and relative volume deformation.As focal adhesion progresses,single cell compression profiles indicate that both membrane and cytoskeleton stiffen,while spreading increases especially from focal complex to focal adhesion.Correspondingly,AFM imaging reveals morphological geometries of spherical cap,spreading with polygon boundaries,and elongated or polarized spreading.Membrane features are dominated by protrusions of 41-207 nm tall,short rods with 1-6 μm in length and 10.2-80.0 nm in height,and long fibrous features of 31-246 nm tall,respectively.The protrusion is attributed to local membrane folding,and the rod and fibrous features are consistent with bilayer decorating over the F-actin assemblies.Taken collectively,the reassembly of F-actin during focal adhesion formation is most likely responsible for the changes in cellular mechanics,spreading morphology,and membrane structural features. | ZIMMER Christopher C SHI LiFang SHIH YiPing LI JieRen JIN LeeWay LO SuHao LIU GangYu | 2012 | Science China Chemistry2012，55，9： | 1 |
| 3 | Characteristics of Surface Micro-discharge Excited by Pulsed Voltage in Open Air显示文摘The surface micro-discharge(SMD)excited by pulsed voltage in open air is experimentally studied by measuring its voltage,current,emission image,emission spectrum,and ultraviolet(UV)absorption spectrum.It is found that the SMD occurs intermittently in each cycle,and that it consists of many filaments on the surface of grounded electrode.While the applied voltage’s peak value Up increases from 1.75 kV to 7.25 kV,the discharge area keeps expanding until Up≈6 kV,when the discharge occupies all the available area.The emission spectrum of nitrogen metastable N2(C-B)and the density of ozone increase almost linearly.The ozone density decreases hyperbolically downstream of the grounded electrode due to the diffusion in open air.The characteristics of pulsed SMD are presented and analyzed as well,which may be referred by further investigations. | LI Dong BAI Chuangfei WANG Xiaohua KONG Gangyu LIU Dingxin | 2013 | 高电压技术2013，39，9： | 0 |
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