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| 1 | Effects of icariin on cGMP-specific PDE5 and cAMP-specific PDE4 activities显示文摘Aim:To clarify the mechanism of the therapeutic action of icariin on erectlile dysfunction(ED).Methods:PDE5 was isolated from the human platelet and PDE4 form the rat liver tissue using the FPLC system (Pharmacia,Milton Keynes,UK)and the Mono Q column.The inhibitory effects of icariin on PDE5 and PDE4 activities were investigated by the two-step radioisotope procedure with [^3H]-c GMP/[^3H]-cAMP.Papaverine served as the control drug.Results:Icariin and papaverine showed dose-dependent inhibitory effects on PDE5 and PDE4 activities.The IC50 of Icariin and papaverine on PDE5 were 0.432μ mol/L and 0.680μmol/L,respectively and those on PDE4,73.50μmol/L and 3.07μmol/L,respectively.The potencies of selectivity of icariin and papaverine on PDE5(PDE4/PDE5 of IC50)were 167.67 times and 4.54 times,respectively.Conclusion:Icariin is a cGMP-specific PDE5 inhibitor that may be developed into an oral effective agent for the treatment of ED. | ZC Xin EK Kim CS Lin WJ Liu L Tian YM Yuan J Fu | 2003 | Asian Journal of Andrology2003，5，1： | 38 |
| 2 | Recognition and implication of eclogite in the western Altun Mountains, Xinjiang显示文摘SINCE high-pressure metapelitic rocks have been discovered in the Beiketan area of eastern Al-tun Mountains,a set of eclogite has been recognized in south of Jianggesayi,western Altun.The eclogite,associated with quartz-garnet-clinopyroxenite and metamorphosed retrogradelyto garnet-amphibolite partially,is exposed in the Proterozoic meso- to hypometamorphic rocksand can be traced eastward to northwest of Mangya asbestos deposit,for about 200 | Liu, L Che, ZC Luo, JH Wang, Y Gao, ZJ | 1997 | Chinese Science Bulletin1997，42，11： | 33 |
| 3 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 4 | Intraspinal transplantation of CD34+ human umbilical cord blood cells after spinal cord hemisection injury improves functional recovery in adult rats显示文摘 | Zhao,ZM Li,HJ Liu,HY Lu,SH Yang,RC Zhang,QJ Han,ZC | 2005 | 中国生物学文摘2005，19，3： | 2 |
| 5 | Circulating Th1 and Th2 cytokines in patients infected with hepatitis C virus 显示文摘 | Fan XG Liu WE Wang ZC | 1998 | Med Inflamm1998，7，： | 2 |
| 6 | Hemangiopoietin, a novel human growth factor for the primitive cells of both hematopoietic and endothelial cell lineages显示文摘 | Liu,YJ Lu,SH Xu,B Yang,RC Ren,Q Liu,B Lu,M Yan,FY Han,ZB Han,ZC | 2005 | 中国生物学文摘2005，19，3： | 2 |
| 7 | Increased umbilical cord plasma interleukin-1 beta levels was correlated with adverse outcomes of neonatal hypoxic-ischemic encephalopathy显示文摘 | Liu J Feng ZC | 2010 | J Trop Pediatr2010，56，3： | 1 |
| 8 | Tumor necrosis factor-like weak in- ducer of apoptosis (TWEAK) mediates p38 mitogen-activated pro- tein kinase activation and signal transduction in peripheral blood mononuclear cells from patients with lupus nephritis 显示文摘 | Liu ZC Zhou QL Liu XZ | 2012 | Inflamma- tion2012，35，3： | 1 |
| 9 | In vitro study of the relationship between the structure of ginsenoside and its antioxidative or prooxidative activity in free radical induced hemolysis of human erythrocytes显示文摘 | Luo XY Liu GZ Chen YP Wang ZC Sun YX | 2003 | J Agric Food Chem2003，51，： | 1 |
| 10 | Effect of the cord pre- tension of the Dynesys dynamic stabilization system on the biomechanics of the lumbar spine: a finite element analysis显示文摘 | Liu CL Zhong ZC Hsu HW | 2011 | Eur Spine J2011，20，11： | 1 |
| 11 | Influence of Dynesys system screw profile on adjacent segment and screw显示文摘 | Liu CL Zhong ZC Shih SL | 2010 | J Spinal Disord Tech2010，23，6： | 1 |
| 12 | The incidence rate of premature rupture of membranes and its influence on fetal, neonatal health:a report from mainland China显示文摘 | Liu J Feng ZC Wu J | | J Trop Pediatr0，56，1： | 1 |
| 13 | The significance of changes in serum tumor necrosis factor (TNF) activity in severely burned patients显示文摘 | Liu XS Lou ZH Yang ZC | 1994 | Burns1994，20，1： | 1 |
| 14 | Exercise reduces adipose tissue via cannabinoid receptor type 1 which is regulated by peroxisome proliferator-activated receptor-delta 显示文摘 | Yah ZC Liu DY Zhang LL | 2007 | Biochem Biophys Res Com mun2007，354，2： | 1 |
| 15 | Effects of shade treatments on the photosynthetic capacity, chlorophyll fluoreeeence, and chlorophyll content of Tetrastigma hemsleyanum Dies et Gilg 显示文摘 | Dai YJ Shen ZC Liu Y | 2009 | Enviromental and Experimental Botany2009，65，17： | 1 |
| 16 | Advanced glycation end-products activate the reninangiotensinsystem through the RAGE/PI3-K signaling pathwayin podocytes显示文摘 | Cheng CL Tang Y Zheng Z Liu X Ye ZC Wang C Lou TQ | 2012 | Clin Invest Med2012，35，5： | 1 |
| 17 | Changes in pulmonary arterial pressure in term-infants with hypoxic-ischemic encephalopathy 显示文摘 | Liu J Feng ZC | 2009 | Pediair Int2009，51，6： | 1 |
| 18 | Involvement of Caspase-3 activation in squamocin induced apoptosis in leukemia cell line HL-60显示文摘 | Zhu XF Liu ZC Xie BF | 2002 | Life Sci2002，70，： | 1 |
| 19 | High-flow pria pism:Superselec-tive caverno us artery embolization with mieroeoils显示文摘 | Liu BX Xin ZC Zou YH | 2008 | Urology2008，72，3： | 1 |
| 20 | Functional studies of a novel cncogene TGM3 in human esophageal squaraous cell carcinoma显示文摘 | Liu W Yu ZC Cao WF | | 0，，24： | 1 |
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