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| 1 | Total mercury and methylmercury and selenium in mercury polluted areas in Guizhou Province,China显示文摘 | Horvat M Nolde N Fajon V Jereb V Logar M Lojen S Jacimovic R Falnoga I Qu Liya Faganeli J DrobneD | 2003 | Science of Total Environment2003，304，： | 1 |
| 2 | Effects of drinking water supplementation with Lactobacillus reuteri,and a mixture of reuterin and microcin J25 on the growth performance,caecal microbiota and selected metabolites of broiler chickens显示文摘Background:Since the overuse of antibiotics in animal production has led to a selection of antibiotic-resistant pathogens that affect humans and animals as well.Scientists are therefore searching for novel natural alternatives to antibiotics.In this study Lactobacillus reuteri and a combination of reuterin and microcin J25(RJ)were evaluated as promoters of growth and modulators of the cecal microbiota and metabolite profiles in broiler chickens.Oneday-old Cobb 500 male broilers were distributed to 8 treatments:negative control(without antibiotic),positive control(bacitracin),three concentrations of RJ and three doses of L.reuteri plus glycerol.The birds(2176,34 per pen,8 pens per treatment)were reared for 35 d.Results:The body weight of the bacitracin and 5 mmol/L reuterin combined with 0.08μmol/L microcin J25(10RJ)treatment group was significantly higher than that of the negative control group(P<0.05).L.reuteri had no significant effect on broiler growth.MiSeq high-throughput sequencing of 16S rRNA showed clustering of cecal microbial operational taxonomic unit diversity according to treatment.The influence of bacitracin and 10RJ on bacterial community overall structure was similar.They promoted Ruminococcaceae,Lachnospiraceae and Lactobacillaceae,increased the relative abundance of Faecalibacterium and decreased the abundance of Bacteroides and Alistipes,while the negative control condition favored Bacteroidaceae and Rikenellaceae.Furthermore,10RJ increased the concentration of short-chain fatty acid in the cecum and changed the metabolome overall.Conclusions:These overall suggest that 10RJ can promote a host-friendly gut environment by changing the cecal microbiome and metabolome.This combination of natural antimicrobial agents in the drinking water had a positive effect on broiler growth and may be suitable as an alternative to antibiotic growth promoters. | Liya Zhang Laila Ben Said Nadège Hervé Séverine Zirah Moussa Sory Diarra Ismail Fliss | 2022 | Journal of Animal Science and Biotechnology2022，13，4： | 1 |
| 3 | Thermal shock resistance of the porous Al2O3/ZrO2 ceramics prepared by gelcasting显示文摘 | LIYA S MINGJIAN L Xiaozhen L | 2007 | Mater Res Bull2007，42，12： | 1 |
| 4 | Detection of simian T cell leukemia virus type Ⅰ infection in seronegative macaques 显示文摘 | Vladimir L Patricia N Liya S | 1997 | AIDS Rearch and Human Retroviruses1997，13，13： | 1 |
| 5 | Effects of fruit ellagitannin extracts, ellagic acid, and their colonic metabolite, urolithin A, on wnt signaling 显示文摘 | Meenakshi S Liya L Jeremy C | 2010 | J Agric Food Chem2010，58，： | 1 |
| 6 | Structure and morphology transition of CeP O4coatings on alumina fibers显示文摘 | Bo L Liya S Xiaozhen L | 2000 | Journal of materials science letters2000，19，4： | 1 |
| 7 | NADPH oxidase and aging drive microglial activation,oxidative stress and dopaminergic neurodegeneration following systemic LPS administration显示文摘 | QIN Liya LIU Yuxin HONG J S | 2013 | Glia2013，61，6： | 1 |
| 8 | Nanocrystalline ultra-high surface area magnesium oxide as a selective base catalyst显示文摘 | Ryan Richards Ravichandra S Mulukutla liya Mishakov | 2001 | Scripat Mater2001，44，： | 1 |
| 9 | Different Sensitivities to Apoptotic Induction by Camptothecin between Normal and Senescent Lens Epithelial Cells显示文摘Purpose: To investigate whether normal and senescent lens epithelial cells have different defense abilities to apoptotic induction factor in vitro.Methods: Rabbit lens epithelial cells were cultured, passed. When reaching confluence, cells from the first and seventh passage were stained by x-gal staining to detect cell senescence. Cell apoptosis was detected by TUNEL(Roche).10μmol/L camptothecin was used to induce cell apoptosis from the lens epithelial cells of the first and seventh passage to distinguish different sensitivities to apoptotic induction factor between normal and senescent cells.Results: The senescent cells (41.17% ± 5.24% ) were detected in the lens epithelial cell culture of the seventh passage, which are higher than those of the first passage (0.98% ±0. 39% ). There was no apoptotic cell detected in the cell cultures undisturbed. Exposure of the first passage cells to camptothecin resulted in death of approximately 23.87% ± 3.45% of the cells during a 36 hour exposure period. In contrast, significantly more lens epithelial cells died through the apoptosis (38.29% ±4. 01% ) from the seventh passage.Conclusion: Senescent cells increased with cell passage. Senescence lens epithelial cells do not undergo apoptosis if they were not disturbed. But the vulnerabilities to apoptotic induction between health and senescence cells were different. | Haike Guo, Haiying Jin, Liya Wang, Hongyang Zhang, Xin YangDepartment of Ophthalmology, Guangdong Provincial People’s Hospital, Guangzhou 510080, ChinaThe First Affiliated Hospital of Medical College, Jinan University, Guangzhou 510632, ChinaHenan Institute of Ophthalmology, Zhengzhou 450003, China | 2002 | 眼科学报2002，18，3： | 1 |
| 10 | Determination of bisphenol A and naphthols in river water samples by capillary zone eleetrophoresis af- ter cloud point extraction 显示文摘 | Zhong Shuxian Tan S N Ge Liya | 2011 | Talanta2011，85，1： | 1 |
| 11 | Connective tissue growth factor differentially binds to members of the cystine knot superfamily and potentiates platelet-derived growth factor-B signaling in rabbit corneal fibroblast cells显示文摘AIM:To study the binding of connective tissue growth factor(CTGF) to cystine knot-containing ligands and how this impacts platelet-derived growth factor(PDGF)-B signaling. METHODS:The binding strengths of CTGF to cystine knot-containing growth factors including vascular en-dothelial growth factor(VEGF)-A,PDGF-B,bone morphogenetic protein(BMP)-4,and transforming growth factor(TGF)-β1 were compared using the LexA-based yeast two-hybrid system. EYG48 reporter strain that carried a wild-type LEU2 gene under the control of Lex A operators and a lac Z reporter plasmid(p80plac Z) containing eight high affinity Lex A binding sites were used in the yeast two-hybrid analysis. Interactions between CTGF and the tested growth factors were evaluated based on growth of transformed yeast cells on selective media and colorimetric detection in a liquid β-galactosidase activity assay. Dissociation constants of CTGF to VEGF-A isoform 165 or PDGF-BB homo-dimer were measured in surface plasma resonance(SPR) analysis. CTGF regulation in PDGF-B presentation to the PDGF receptor β(PDGFRβ) was also quantitatively assessed by the SPR analysis. Combinational effects of CTGF protein and PDGF-BB on activation of PDGFRβ and downstream signaling molecules ERK1/2 and AKT were assessed in rabbit corneal fibroblast cells by Western analysis. RESULTS:In the LexA-based yeast two-hybrid system,cystine knot motifs of tested growth factors were fused to the activation domain of the transcriptional factor GAL4 while CTGF was fused to the DNA binding domain of the bacterial repressor protein Lex A. Yeast cotransformants containing corresponding fusion proteins for CTGF and all four tested cystine knot motifs survived on selective medium containing galactose and raffinose but lacking histidine,tryptophan,and uracil. In liquid β-galactosidase assays,CTGF expressing cells that were co-transformed with the cystine knot of VEGF-A had the highest activity,at 29.88 ± 0.91 fold above controls(P < 0.01). Cells containing the cystine knot of BMP-4 expressed the second most activity,with a 24.77 ± 0.47 fold increase(P < 0.01). Cells that contained the cystine knot of TGF-β1 had a 3.80 ± 0.66 fold increase(P < 0.05) and the ones with the cystine knot of PDGF-B had a 2.64 ± 0.33 fold increase of β-galactosidase activity(P < 0.01). Further SPR analysis showed that the association rate between VEGF-A 165 and CTGF was faster than PDGF-BB and CTGF. The calculated dissociation constant(KD) of CTGF to VEGF165 and PDGF-BB was 1.8 and 43 nmol/L respectively. PDGFBB ligand and PDGFRβ receptor formed a stable complex with a low dissociation constant 1.4 nmol/L. Increasing the concentration of CTGF up to 263.2 nmol/L significantly the ligand/receptor binding. In addition,CTGF potentiated phosphorylation of PDGFRβ and AKT in rabbit corneal fibroblast cells stimulated by PDGFBB in tissue culture condition. In contrast,CTGF did not affect PDGF-B induced phosphorylation of ERK1/2.CONCLUSION:CTGF has a differential binding affinity to VEGF-A,PDGF-B,BMP-4,and TGF-β. Its weak association with PDGF-B may represent a novel mechanism to enhance PDGF-B signaling. | Liya Pi Pei-Yu Chung Sriniwas Sriram Masmudur M Rahman Wen-Yuan Song Edward W Scott Bryon E Petersen Gregory S Schultz | 2015 | World Journal of Biological Chemistry2015，6，4： | 0 |
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