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| 1 | Construction of Dual-Biofunctionalized Chitosan/Collagen Scaffolds for Simultaneous Neovascularization and Nerve Regeneration显示文摘Biofunctionalization of artificial nerve implants by incorporation of specific bioactive factors has greatly enhanced the success of grafting procedures for peripheral nerve regeneration.However,most studies on novel biofunctionalized implants have emphasized the promotion of neuronal and axonal repair over vascularization,a process critical for long-term functional restoration.We constructed a dual-biofunctionalized chitosan/collagen composite scaffold with Ile-Lys-Val-Ala-Val(IKVAV)and vascular endothelial growth factor(VEGF)by combining solution blending,in situ lyophilization,and surface biomodification.Immobilization of VEGF and IKVAV on the scaffolds was confirmed both qualitatively by staining and quantitatively by ELISA.Various single-and dual-biofunctionalized scaffolds were compared for the promotion of endothelial cell(EC)and Schwann cell(SC)proliferation as well as the induction of angiogenic and neuroregeneration-associated genes by these cells in culture.The efficacy of these scaffolds for vascularization was evaluated by implantation in chicken embryos,while functional repair capacity in vivo was assessed in rats subjected to a 10mm sciatic nerve injury.Dual-biofunctionalized scaffolds supported robust EC and SC proliferation and upregulated the expression levels of multiple genes and proteins related to neuroregeneration and vascularization.Dual-biofunctionalized scaffolds demonstrated superior vascularization induction in embryos and greater promotion of vascularization,myelination,and functional recovery in rats.These findings support the clinical potential of VEGF/IKVAV dual-biofunctionalized chitosan/collagen composite scaffolds for facilitating peripheral nerve regeneration,making it an attractive candidate for repairing critical nerve defect.The study may provide a critical experimental and theoretical basis for the development and design of new artificial nerve implants with excellent biological performance. | Guicai Li Qi Han Panjian Lu Liling Zhang Yuezhou Zhang Shiyu Chen Ping Zhang Luzhong Zhang Wenguo Cui Hongkui Wang Hongbo Zhang | 2020 | Research2020，，1： | 2 |
| 2 | Dispersion of the Mechanical Parts Performance Indicators Based on the Concept of Random Vector显示文摘To solve the precision and reliability problem of various machinery equipments and military vehicles,some military organisations,the industrial sector and the academia at home and abroad begin to pay attention to the statistical distribution of machining dimensions,material properties and service loads,and the system reliability optimization design with constraints and reliability optimization design of various mechanical parts is studied in this way.However,the above researches focus on solving the strength and the life problem,and no studies have been done on the discrete degree and discrete pattern of other performance indicators.The concept of using a random vector to describe the mechanical parts performance indicators is presented;characteristics between the value of the vector variance matrix determinant and the sum of the diagonal covariance matrix in describing the performance indicators of vector dispersion are studied and compared.A clutch diaphragm spring is set as an example,the geometric dimension indicator is described with random vector,and the applicability of using variance matrix determinant and variance matrix trace of geometric dimension vector to describe discrete degree of random vector is studied by using Monte-Carlo simulation method and component discrete degree perturbation method.Also,the effects of different components of diaphragm spring geometric dimension vector on the value of covariance matrix determinant and the sum of covariance matrix diagonal of diaphragm spring performance indicators vector are analyzed.The present study shows that the impacts of the dispersion of diaphragm spring cone angle on every performance dispersion are all ranked first,and far exceed that of other dimension dispersion.So it must be strictly controlled in the production process.The result of the research work provides a reference for the design of diaphragm spring,and also it presents a proper method for researching the performance of other mechanical parts. | XIA Changgao ZHU Pei ZHANG Meng GAO Xiang LU Liling | 2012 | Chinese Journal of Mechanical Engineering2012，25，1： | 1 |
| 3 | Titanate nanotubes: preparation, characterization, and application in the detection of dopamine显示文摘 | Liling Niu Mingwang Shao Sheng Wang Lei Lu Huazhong Gao Jun Wang | 2008 | Journal of Materials Science2008，，5： | 1 |
| 4 | Lactate Dehydrogenase as a Biomarker for Prediction of Refractory Mycoplasma pneumoniae Pneumonia in Children显示文摘 | Aizhen Lu Chuankai Wang Xiaobo Zhang Libo Wang Liling Qian | 2015 | Respiratory Care2015，，10： | 1 |
| 5 | A comprehensive profile of TCF1^(+)progenitor and TCF1−terminally exhausted PD-1^(+)CD8^(+)T cells in head and neck squamous cell carcinoma:implications for prognosis and immunotherapy显示文摘The heterogeneity of exhausted T cells(Tex)is a critical determinant of immune checkpoint blockade therapy efficacy.However,few studies have explored exhausted T cell subpopulations in human cancers.In the present study,we examined samples from two cohorts of 175 patients with head and neck squamous cell cancer(HNSCC)by multiplex immunohistochemistry(mIHC)to investigate two subsets of Tex,CD8+PD1+TCF1+progenitor exhausted T cells(TCF1+Texprog)and CD8+PD1+TCF1−terminally exhausted T cells(TCF1−Texterm).Moreover,fresh tumor samples from 34 patients with HNSCC were examined by flow cytometry and immunohistochemistry to further investigate their properties and cytotoxic capabilities and their correlation with regulatory T cells(Tregs)in the tumor immune microenvironment(TIME).mIHC and flow cytometry analysis showed that TCF1−Texterm represented a greater proportion of CD8+PD1+Tex than TCF1+Texprog in most patients.TCF1+Texprog produced abundant TNFα,while TCF1−Texterm expressed higher levels of CD103,TIM-3,CTLA-4,and TIGIT.TCF1−Texterm exhibited a polyfunctional TNFα+GZMB+IFNγ+phenotype;and were associated with better overall survival and recurrence-free survival.The results also indicated that larger proportions of TCF1−Texterm were accompanied by an increase in the proportion of Tregs.Therefore,it was concluded that TCF1−Texterm was the major CD8+PD1+Tex subset in the HNSCC TIME and that these cells favor patient survival.A high proportion of TCF1−Texterm was associated with greater Treg abundance. | Dikan Wang Juan Fang Shuqiong Wen Qunxing Li Jinming Wang Lisa Yang Wenxiao Dai Huanzi Lu Junyi Guo Zhongyan Shan Wenqiang Xie Xiangqi Liu Liling Wen Jie Shen Anxun Wang Qianming Chen Zhi Wang | 2022 | International Journal of Oral Science2022，14，1： | 0 |
| 6 | B effects on crystallization and magnetic properties of Pr_(9.44)Fe_(90.56-x)B_x(x=7.16, 4.76) nanocomposite magnets显示文摘Magnetic properties, phase evolution and microstructures of Pr9.44Fe90.56?xBx (x=7.16 and 4.76) nanocomposite magnets have been investigated. Both as-spun ribbons exhibit two-step demagnetization curves, indicating the feature of partial crystallization. By using di?erential scanning calorimeter (DSC) measurements, remained amorphous phase of ribbon with x=7.16 shows a higher crystallization temperature. At optimum annealing temperature of 650 ?C for 10 min, better magnetic properties, Mr=1.01 T, jHc=796 kA/m and (BH)max=136 kJ/m3 were obtained in the sample with x=4.76. Transmission electron microscopy (TEM) observation confirms that the sample with x=4.76 possesses much finer grain size, which causing a better exchange coupling between soft and hard magnetic grains. | Caiyin YOU Na TIAN Zhengxin LU Liling GE Xiaotian JING | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，5： | 0 |
| 7 | Influence of Organic Cations on the Crystal and Electronic Structures of Two-dimensional Lead Iodide Perovskites显示文摘The crystal structures and electronic structures(including band gap,project density of states,partial charge density,effective mass and electron localization function)of the 2D lead iodide perovskites hybrids with different organic spacer cations of 4-fluorophenylethanaminium(4F-PEA^(+)),ethanolamine(EA^(+)),thienylethylamine(TEA^(+))were investigated using first-principles calculations.It was found the higher dipole moment,the stronger the hydrogen bonding between the organic amino and iodide in the inorganic layer,and the larger the[PbI_(6)]^(4-)octahedral distortions in these crystal structure.Further quantifying the degree of the distortions using OctaDist software showed that the distortion of adjacent[PbI_(6)]^(4-)octahedra had a decisive effect on the band gap.Specifically,the greater deviation of Pb-I-Pb bond angles from 180°,together with the larger distortion of multiple[PbI_(6)]^(4-)octahedron resulted in a wider band gap,which was verified by calculated band gap using different DFT methods.The results outlined the relationships of hydrogen bonding,ocathedra distortion and band structure in 2D perovskites,highlighting the importance of the cations on the structural tuning and optoelectronic properties. | 杜定谨 甘小燕 LU Shun ZHENG Wei GUO Liling LIU Hanxing | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，3： | 0 |
| 8 | 能量匮乏条件下Snf1-Mec1-Atg1通过控制线粒体呼吸作用调控细胞自噬的发生显示文摘文章简介m TOR和AMPK两个信号分子在细胞自噬的发生过程中扮演着非常重要的调控作用。近年来,人们对于m TOR信号分子如何调控细胞自噬的研究比较清楚,但是对于在能量匮乏条件下,AMPK信号分子如何调控细胞自噬的发生尚不清楚。 | Cong Yi Jingjing Tong Puzhong Lu Yizheng Wang Jinxie Zhang Chen Sun Kangning Yuan Renyu Xue Bing Zou Nianzhong Li Shuhua Xiao Chong Dai Yuwei Huang Liling Xu Lin Li She Chen Di Miao Haiteng Deng Hongliang Li 俞立 | 2018 | 科学新闻2018，0，4： | 0 |
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