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| 1 | New gene therapy strategies for hepatic fibrosis显示文摘The liver is the largest internal organ of the body, which may suffer acute or chronic injury induced by many factors, leading to cirrhosis and hepatocarcinoma. Cirrhosis is the irreversible end result of fibrous scarring and hepatocellular regeneration, characterized by diffuse disorganization of the normal hepatic structure, regenerative nodules and fibrotic tissue. Cirrhosis is associated with a high co-morbidity and mortality without effective treatment, and much research has been aimed at developing new therapeutic strategies to guarantee recovery. Liver-based gene therapy has been used to downregulate specific genes, to block the expression of deleterious genes, to delivery therapeutic genes, to prevent allograft rejection and to augment liver regeneration. Viral and non-viral vectors have been used, with viral vectors proving to be more efficient. This review provides an overview of the main strategies used in liver-gene therapy represented by non-viral vectors, viral vectors, novel administration methods like hydrodynamic injection, hybrids of two viral vectors and blocking molecules, with the hope of translating findings from the laboratory to the patient′s bed-side. | Adriana M Salazar-Montes Luis D Hernández-Ortega Martha S Lucano-Landeros Juan Armendariz-Borunda | 2015 | World Journal of Gastroenterology2015，21，13： | 12 |
| 2 | Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma显示文摘AIM: To evaluate the efficacy and the safety of azathioprine (AZA) and buthionine sulfoximine (BSO) bylocalized application into HepG2 tumor in vivo.METHODS: Different hepatoma and colon carcinoma cell lines (HepG2, HuH7, Chang liver, LoVo, RKO, SW-48, SW-480) were grown in minimal essencial medium supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution and maintained in a humidified 37 ℃ incubator with 5% CO2. These cells were pretreated with BSO for 24 h and then with AZA for different times. We examined the effects of this combination on some proteins and on cellular death. We also studied the eff icacy and the safety of AZA (6 mg/kg per day) and BSO (90 mg/kg per day) in HepG2 tumor growth in vivo using athymic mice. We measured safety by serological markers such as aminotransferases and creatine kinase.RESULTS: The in vitro studies revealed a new mechanism of action for the AZA plus BSO combination in the cancer cells compared with other thiopurines (6-mercaptopurine, 6-methylmercaptopurine, 6-thioguanine and 6-methylthioguanine) in combination with BSO. The cytotoxic effect of AZA plus BSO in HepG2 cells resulted from necroptosis induction in a mitochondrial-dependent manner. From kinetic studies we suggest that glutathione (GSH) depletion stimulates c-Jun amino-terminal kinase and Bax translocation in HepG2 cells with subsequent deregulation of mitochondria (cytochrome c release, loss of membrane potential), and proteolysis activation leading to loss of membrane integrity, release of lactate dehydrogenase and DNA degradation. Some of this biochemical and cellular changes could be reversed by N-acetylcysteine (a GSH replenisher). In vivo studies showed that HepG2 tumor growth was inhibited when AZA was combined with BSO.CONCLUSION: Our studies suggest that a combination of AZA plus BSO could be useful for localizedtreatment of hepatocellular carcinoma as in the currently used transarterial chemoembolization method. | Borja Hernández-Breijo Jorge Monserrat Sara Ramírez-Rubio Eva P Cuevas Diana Vara Inés Díaz-Laviada M Dolores Fernández-Moreno Irene D Román Javier P Gisbert Luis G Guijarro | 2011 | World Journal of Gastroenterology2011，17，34： | 2 |
| 3 | 14-day triple, 5-day concomitant, and 10-day sequential therapies for Helicobacter pylori infection in seven Latin American sites: a randomised trial显示文摘 | E Robert Greenberg Garnet L Anderson Douglas R Morgan Javier Torres William D Chey Luis Eduardo Bravo Ricardo L Dominguez Catterina Ferreccio Rolando Herrero Eduardo C Lazcano-Ponce María Mercedes Meza-Montenegro Rodolfo Pe?a Edgar M Pe?a Eduardo Salazar- | 2011 | The Lancet2011，，9790： | 2 |
| 4 | Cartilage regeneration using principles of tissue engineering显示文摘 | LUIS A SOLCHAGA P D VICTOR M et al | 2001 | Clin Orthop Res2001，，： | 1 |
| 5 | Automatic bandwidth selection of fuzzy membership fnctions显示文摘 | Cheng H D Lui Y M | 1997 | Informations Science1997，103，： | 1 |
| 6 | Chromenes of polyketide oridin from Peperomia villipetiola 显示文摘 | Karina J M S Guillermo E D P Luis R L | 2005 | Phytochemistry2005，66，： | 1 |
| 7 | Caspase-9, caspase-3 and easpase-7 have distinct roles during in- trinsic apoptosis显示文摘 | MATTHEW B LUIS R M REBEKA L D G | 2013 | BMC Cell Biology2013，，14： | 1 |
| 8 | A wireless controller to enhance dynamic performance of parallel inverters in distributed generation system显示文摘 | GUERRERO J M LUIS GARCIA D E V JOSE M | 2004 | IEEE Transactions on Power Electronics2004，19，5： | 1 |
| 9 | A novel approach to microcalcification detection using fuzzy logic technique显示文摘 | Cheng H D Lui Y M Freimanis R I | 1998 | IEEE Transactions on Medical Imaging1998，17，3： | 1 |
| 10 | Improving passive filter compensation performance with active techniques显示文摘 | Darwin R Luis M Juan W D | 2003 | IEEE trans on industrial electronics2003，50，1： | 1 |
| 11 | Epoxy networks containing large mass fractions of a monofunctional polyhedral oligomeric silsesquioxane (POSS)显示文摘 | Abad J M Luis B Fasce P D | 2003 | Macromolecules2003，36，9： | 1 |
| 12 | DCAR: distributed coding-aware routing in wireless networks显示文摘 | LE J L LUI J C S CHIU D M | 2008 | IEEE Transantion on Mobile Computing2008，9，4： | 1 |
| 13 | Total homocysteine levels relation with chronic complications of diabetes,body composition,and other cardiovascular risk factors in a population of patients with diabetes mellitus type 2显示文摘 | de Luis D A Fernandez N Arranz M L | | 0，，01： | 1 |
| 14 | An analysis of productive efficiency and innovation activity using DEA: an application to spain' s wood - based industry 显示文摘 | Luis D B Casimiro A H Margarita M Jacinto G P | 2006 | Forest Policy and Economics2006，，8： | 1 |
| 15 | 显示文摘 | Luis da S Z Ronaldo D M Rogerio | 2002 | Vacuum2002，65，： | 1 |
| 16 | Evaluation of the mini-nutritional assessment short-form (MNA-SF) among institutionalized older patients in Spain 显示文摘 | LUIS D A D MONGIL R L SAGRADO M G | 2011 | Nutr Hosp2011，26，6： | 1 |
| 17 | Role of scirpus laucustris in bacteria and nutrient removal from wastewater 显示文摘 | Solt F Garcia M Luis D | 1999 | Wat Sci Tech1999，40，3： | 1 |
| 18 | Response ofpseudomonasputida F1 cultures to fluctuating toluene loads and operational failures in suspended growth bioreaetors显示文摘 | Luis F D Sergio B | 2008 | Biodegradation2008，19，： | 1 |
| 19 | An analysis of productive efficiency and innovation activity using DEA: An application to Spain's wood-based industry 显示文摘 | LUIS D B CASIMIRO H A MARGARITA M | 2006 | Forest Policy and Economics2006，8，7： | 1 |
| 20 | Seasonal dynamics of wood formation in Pinus halepensis from dry and semi-arid ecosystems in Spain显示文摘 | LUIS M D GRICAR J CUFAR K | 2007 | IAWA Journal2007，28，： | 1 |
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