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| 1 | Highly efficient derivation of ventricular cardiomyocytes from induced pluripotent stem cells with a distinct epigenetic signature显示文摘从 pluripotent 干细胞导出的 Cardiomyocytes 能在药测试,疾病建模和基于房间的治疗被使用。没有 procardiogenic 生长因素,然而,从 pluripotent 干细胞的 cardiomyogenesis 的效率通常是低的,产生 cardiomyocyte 人口是异构的。这里,我们证明导致的 pluripotent 干细胞( iPSCs )能从鼠科的室的 myocytes ( VM )被导出,并且与从各种各样的体的房间类型导出的 iPSCs 的另外的报告一致,自发地作为与遗传上匹配的胚胎的干细胞(转换字符)或 iPSCs 相比区分 cardiomyocytes 进跳动的显著地更高的倾向从尾巴尖端成纤维细胞导出的 导出VM 的 iPSCs ( ViPSCs )展览。惊人地,导出 ViPSC 的 cardiomyocytes 显示的多数室的显型。在 ViPSCs 的提高的室的 myogenesis 在区别的早阶段经由心血管的祖先的增加的数字被调停。以便从 ViPSCs 调查提高的室的 myogenesis 的机制,我们执行了全球基因表示和 DNA methylation 分析,它揭示了可以涉及在 pluripotent 干细胞指定 VM 命运的不同 epigenetic 签名。 | Huansheng Xu B Alexander Yi Hao Wu Christoph Bock Hongcang Gu Kathy O Lui Joo-Hye C Park Ying Shao Alyssa K Riley Ibrahim J Domian Erding Hu Robert Willette John Lepore Alexander Meissner Zhong Wang Kenneth R Chien | 2012 | Cell Research2012，22，1： | 7 |
| 2 | pRB expression in esophageal mucosa of individuals at high risk for squamous cell carcinoma of the esophagus显示文摘AIM: To investigate the pRb expression in a large group of patients with history of chronic exposure to the main risk factors for development of squamous cell carcinoma of the esophagus. METHODS: One hundred and seventy asymptomatic individuals at high risk for esophageal squamous cell carcinoma (consumption of more than 80 g of ethanol and 10 cigarettes/d for at least 10 years) underwent upper gastrointestinal endoscopy with biopsies of the esophageal mucosa. As a control group, specimens of esophageal mucosa obtained from 20 healthy subjects were also studied. Immunohistochemical assessment of the tissues was performed using a monoclonal antibody anti-pRB protein. RESULTS: Absence of the pRB staining, indicating loss of RB function, was observed in 33 (19.4%) of the individuals at risk for esophageal cancer, but in none of the healthy controls (P < 0.02). Loss of pRb expression increased in a stepwise fashion according to the severity of the histological findings (P < 0.005): normal mucosa (11/97 or 11.3%), chronic esophagitis (17/60 or 28.3%), low-grade dysplasia (3/10 or 30%), high-grade dysplasia 1/2 or 50%) and squamous cell carcinoma (1/1 or 100%). CONCLUSION: Our findings suggest that abnormal expression of the pRB protein may be implicated in the process of esophageal carcinogenesis. Additional studies are warranted to define the role of the pRBprotein as a biomarker for development of esophageal squamous cell carcinoma in individuals at high risk for this malignancy. | Simone S Contu Paulo C Contu Daniel C Damin Renato B Fagundes Fabiano Bevilacqua Aline S Rosa Joo C Prolla Luis F Moreira | 2007 | World Journal of Gastroenterology2007，13，11： | 5 |
| 3 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 4 | Patterns of Known and Novel Small RNAs in Human Cervical Cancer 显示文摘 | LUI W O POURMAND N PATYERSON B K | 2007 | Cancer Research2007，67，13： | 1 |
| 5 | Torque Ripple Minimization in a Switched Reluctance Drive by Neuro-fuzzy Compensation 显示文摘 | Luis O A P Henriques Luis G B Rolim Walter I Suemitsu etal | 2000 | IEEE Transactions on magnetics2000，36，5： | 1 |
| 6 | A new generation- recombination model for device simulation including the Poole-Frenkel effect and phonon assisted 显示文摘 | LUI O K B MIGLIORATO P | 1997 | Sol Sta Elec1997，41，4： | 1 |
| 7 | Inequality and deprivation within and between groups:An illustration of European Union countries显示文摘 | Elena B M Luis I O | | 0，，05： | 1 |
| 8 | Torque Ripple Minimization in a Switched Reluctance Drive by Neuro- fuzzy Compensation 显示文摘 | Luis O A P Henriques Lugs G B Rolina Waiter I Suemit su | 2000 | IEEE Transactions on magnetics2000，36，5： | 1 |
| 9 | Sistemas transcurrentes de escala litosferica en el Sur del Peru显示文摘 | Thierry S Javier J Victor C William M Conrado B Michel F Pierrick R Harmuth A Jorge A Luis C Alexander F Ivanoff I Omar L Mirian M Paola M Francis O Yovani O Adan P and Rildo R | | 0，，05： | 1 |
| 10 | On the dynamic allocation of resources using linear prediction of aggregate network traffic显示文摘 | Miguel L G Jose R G Luis O B | | 0，，03： | 1 |
| 11 | Comparative of the inlastic response of base isolated buildings显示文摘 | DANIEL O DORA F LUIS B | 2003 | Earthquake Engineering and Structural Dynamics2003，32，： | 1 |
| 12 | Enzymatic activ-ity of lipase in post - metamorphic phase bullfrogs 显示文摘 | LUIS G T B MARIA G A O WILLIAM C L | 2006 | Sci Agric2006，163，5： | 1 |
| 13 | Inhibition of Pluripotency Networks by the Rb Tumor Suppressor Restricts Reprogramming and Tumorigenesis显示文摘 | Michael S. Kareta Laura L. Gorges Sana Hafeez Bérénice A. Benayoun Samuele Marro Anne-Flore Zmoos Matthew J. Cecchini Damek Spacek Luis F.Z. Batista Megan O’Brien Yi-Han Ng Cheen Euong Ang Dedeepya Vaka Steven E. Artandi Frederick A. Dick Anne Brunet Juli | 2014 | Cell Stem Cell2014，，： | 1 |
| 14 | Glottal source biometricalsignature for voice pathology detection显示文摘 | PEDRO G V ROBERTO F B VICTORIA R B VICTOR N L AGUSTIN A M LUIS M M F RAFAEL M O JUAN I G L | 2009 | Speech Communication2009，51，9： | 1 |
| 15 | Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lake comparison by microscopy and denaturing gradient gel electrophoresis显示文摘 | EMILIO O C HENDRIK S LUIS B | 2000 | Appl Environ Microbiol2000，66，2： | 1 |
| 16 | Performance an alysis of wireless ATM/AAL2 over a burst error channel 显示文摘 | SOPHIA T LUIS O B | 2001 | Electrical and Computer Engineering Canadian Conference2001，1，1： | 1 |
| 17 | Fast-scan cyclic voltammetryscanning electrochemical microscopy显示文摘 | LUIS D B MARIO A A DAVID O | 2007 | Journal of Electroanalytical Chemistry2007，1，1： | 1 |
| 18 | Torque Ripple Minimization in a Switched Reluctance Drive by Neuro-fuzzy Compensa- tion显示文摘 | LUIS O A P HENRIQUES LUIS G B Rolim Walter I Suemit su | 2000 | IEEE Transactions on magnetics2000，36，5： | 1 |
| 19 | Transcription factors in plant defense and stress responses显示文摘 | Karam B Singh Rhonda C Foley Luis O?ate-Sánchez | 2002 | Current Opinion in Plant Biology2002，，5： | 1 |
| 20 | Torque Ripple Minimization in a Switched Reluctance Drive by Neuro-fuzzy Compensation显示文摘 | Luis O A P Henriques Luis G B Rolim Walter I Suemitsu | 2000 | IEEE Transactions on magnetics2000，36，5： | 1 |
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