| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014,20,12: | 3 |
| 12 | Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of Cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday显示文摘Following a large-scale phylogenetic study of the lichenized genus Cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 years.Based on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been sequenced.Species of Cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is provided.The new species are:Cora accipiter Moncada,Madrin˜a´n&Lücking spec.nov.,C.applanata Moncada,Soto-Medina&Lücking spec.nov.,C.arachnodavidea Moncada,Dal Forno&Lücking spec.nov.,C.arborescens Dal Forno,Chaves&Lücking spec.nov.,C.arcabucana Moncada,C.Rodrı´guez&Lücking spec.nov.,C.aturucoa Lücking,Moncada&C.Vargas spec.nov.,C.auriculeslia Moncada,Ya´nez-Ayabaca&Lücking spec.nov.,C.barbifera Moncada,Patin˜o&Lücking spec.nov.,C.boleslia Lücking,E.Morales&Dal Forno spec.nov.,C.caliginosa Holgado,Rivas Plata&Perlmutter spec.nov.,C.campestris Dal Forno,Eliasaro&Spielmann spec.nov.,C.canari Nugra,Dal Forno&Lücking spec.nov.,C.caraana Lücking,Martins&Lucheta spec.nov.,C.casasolana Moncada,R.-E.Pe´rez&Lücking spec.nov.,C.caucensis Moncada,M.Gut.&Lücking spec.nov.,C.celestinoa Moncada,CabreraAmaya&Lücking spec.nov.,C.comaltepeca Moncada,R.-E.Pe´rez&Herrera-Camp.spec.nov.,C.corani Lücking,E.Morales&Dal Forno spec.nov.,C.corelleslia Moncada,A.Sua´rez-Corredor&Lücking spec.nov.,C.crispoleslia Moncada,J.Molina&Lücking spec.nov.,C.cuzcoensis Holgado,Rivas Plata&Perlmutter spec.nov.,C.dalehana Moncada,Madrin˜a´n&Lücking spec.nov.,C.davibogotana Lücking,Moncada&Coca spec.nov.,C.davicrinita Moncada,Madrin˜a´n&Lücking spec.nov.,C.davidia Moncada,L.Vargas&Lücking spec.nov.,C.dewisanti Moncada,A.Sua´rez-Corredor&Lücking spec.nov.,C.dulcis Moncada,R.-E.Pe´rez&Lücking spec.nov.,C.elephas Lücking,Moncada&L.Vargas spec.nov.,C.fuscodavidiana Lücking,Moncada&L.Vargas spec.nov.,C.garagoa Simijaca,Moncada&Lücking spec.nov.,C.gigantea Lücking,Moncada&Coca spec.nov.,C.gomeziana Dal Forno,Chaves&Lücking spec.nov.,C.guajalitensis Lücking,Robayo&Dal Forno spec.nov.,C.hafecesweorthensis Moncada,Lücking&R.Pela´ez spec.nov.,C.haledana Dal Forno,Chaves&Lücking spec.nov.,C.hawksworthiana Dal Forno,P.Nelson&Lücking spec.nov.,C.hochesuordensis Lücking,E.Morales&Dal Forno spec.nov.,C.hymenocarpa Lücking,Chaves&Lawrey spec.nov.,C.imi Lücking,Chaves&Lawrey spec.nov.,C.itabaiana Dal Forno,Aptroot&M.Ca´ceres spec.nov.,C.leslactuca nov.,C.maxima Wilk,Dal Forno&Lücking spec.nov.,C.minutula Lücking,Moncada&Ya´nez-Ayabaca spec.nov.,C.palaeotropica Weerakoon,Aptroot&Lücking spec.nov.,C.palustris Dal Forno,Chaves&Lücking spec.nov.,C.parabovei Dal Forno,Kukwa&Lücking spec.nov.,C.paraciferrii Lücking,Moncada&J.E.Hern.spec.nov.,C.paraminor Dal Forno,Chaves&Lücking spec.nov.,C.pastorum Moncada,Patin˜o&Lücking spec.nov.,C.pichinchensis Paredes,Jonitz&Dal Forno spec.nov.,C.pikynasa J.-M.Torres,Moncada&Lücking spec.nov.,C.pseudobovei Wilk,Dal Forno&Lücking spec.nov.,C.pseudocorani Lücking,E.Morales&Dal Forno spec.nov.,C.putumayensis L.J.Arias,Moncada&Lücking spec.nov.,C.quillacinga Moncada,F.Ortega&Lücking spec.nov.,C.rothesiorum Moncada,Madrin˜a´n&Lücking spec.nov.,C.rubrosanguinea Nugra,Moncada&Lücking spec.nov.,C.santacruzensis Dal Forno,Bungartz&Ya´nezAyabaca,spec.nov.,C.schizophylloides Moncada,C.Rodrı´guez&Lücking spec.nov.,C.smaragdina Lücking,Rivas Plata&Chaves spec.nov.,C.soredavidia Dal Forno,Marcelli&Lücking spec.nov.,C.subdavicrinita Moncada,J.Molina&Lücking spec.nov.,C.suturifera Nugra,Besal&Lücking spec.nov.,C.terrestris Dal Forno,Chaves&Lücking spec.nov.,C.terricoleslia Wilk,Dal Forno&Lücking spec.nov.,C.udebeceana Moncada,R.Pela´ez&Lücking,Moncada&R.Pela´ez spec.Lücking spec.nov.,C.urceolata Moncada,Coca&Lücking spec.nov.,C.verjonensis Lücking,Moncada&Dal Forno spec.nov.,C.viliewoa Lücking,Chaves&Soto-Medina spec.nov.,and C.yukiboa Mercado-Dı´az,Moncada&Lücking spec.nov.Furthermore,the taxonomic status of the recently described or recognized species C.arachnoidea,C.aspera,C.ciferrii,and C.reticulifera,is revised. | Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando Coca Leidy Yasmín Vargas-Mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio Cabrera-Amaya Marcela E.S.Cáceres José Luis Chaves Sionara Eliasaro Martha Cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-Campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta Santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo Camilo Rodríguez Diego Fernando Simijaca Edier Soto-Medina Adriano Afonso Spielmann Alejandra Suárez-Corredor Jean-Marc Torres Carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela Celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh Sikaroodi James D.Lawrey | 2017 | Fungal Diversity2017,,3: | 0 |