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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Geochemistry and petrology of some Protero- zoic banded iron-formations of the Quadrilatero Ferrifero, Minas Gerais, Brazil显示文摘 | Klein C Ladeira E A | 2000 | Economical Geology2000，95，： | 1 |
| 3 | Advanced lung adenocarcinoma in an EGFR-positive patient treated with Erlotinib for 52 months显示文摘 | Ivone Gon?alves Inês Ladeira Ana Castro Ana Antunes Ana Barroso Bárbara Parente | 2013 | Respiratory Medicine Case Reports2013，，： | 1 |
| 4 | Adsorption and desorption of arsenic on an oxisol and its constituents显示文摘 | LADEIRA A C Q CIMINELLI V S T | 2004 | Water Research2004，38，20： | 1 |
| 5 | Analysis of the ge- netic variability of PvMSP-3c among Plasmodium vivax in Brazil- ian field isolates 显示文摘 | Ribeiro RS Ladeira L Rezende AM | 2011 | Mena I Oswaldo Cruz2011，106，1： | 1 |
| 6 | Adsorption and desorption of arsenic on an oxisol and its constituents显示文摘 | LADEIRA A C Q CIMINELLI V S T | 2004 | Water Res2004，38，： | 1 |
| 7 | Genotoxic effects in occupational exposure to formaldehyde: a study in anatomy and pathology laboratories and formaldehyde-resins production显示文摘 | Viegas S Ladeira C Nunes C | 2010 | J Occup Med Toxicol2010，5，： | 1 |
| 8 | Ephed- rine versus phenylephrine:prevention of hypotension during spinal block for cesarean section and effects on the fetus 显示文摘 | Magalhaes E Goveia CS Araujo Ladeira LC | 2009 | Rev Bras Anestesiol2009，59，1： | 1 |
| 9 | Theape- linergic system:the role played in human physiology and pathology and potential therapeutic applications 显示文摘 | Ladeiras - Lopes R Ferreira - Martins J Leite - Moreira AF | 2008 | ArqBras Cardiol2008，90，5： | 1 |
| 10 | Relationship among oxidative DNA damage, gastric mucosal density and the relevan ceof cagA, vacA and iceA genotypes of Helicobacter pylori显示文摘 | Ladeira MS Bueno RC Dos Santos BF | 2008 | Dig Dis Sci2008，53，12： | 1 |
| 11 | Analy- sis of DNA damage induced by aflatoxin BI in dunkin-hart- ley guinea pigs显示文摘 | Miranda D D C Arcari D P Ladeira M S P | 2007 | Mycopathologia2007，163，： | 1 |
| 12 | EpKedrineversus phenylephrine:prevention of hypotension during spinalblock for cesarean section and effects on the fetus显示文摘 | Magalhaes E Goveia CS de Araujo Ladeira LC | 2009 | Rev BrasAnestesiol2009，，： | 1 |
| 13 | Influence of the confinement, soil density and displacement rate on soil-geogrid interaction 显示文摘 | LOPES M L LADEIRA M | 1996 | Geotextiles and Geomembranes1996，14，10： | 1 |
| 14 | Relationship between fracture spacing and bed thickness显示文摘 | Ladeira F L Price N J | 1981 | Journal of Structural Geology1981，3，12： | 1 |
| 15 | Reactivity, performance,color and tenderness of meat from Zehu cattle finished in feedlot显示文摘 | Ribeiro J S Goncalves T M Ladeira M M | 2012 | Revista Brasileira de Zootecnia2012，41，4： | 1 |
| 16 | The apelinergic system : the role played in human physiology and pathology and potential ther- apeutic applications显示文摘 | Ladeiras LR Ferreira MJ Leite MA | 2008 | Arq Bras Cardiol2008，90，： | 1 |
| 17 | Studies on Dieffenbachia picta Schott:Toxic effects in Guinea pigs显示文摘 | Ladeira (A) M Anrade S O Sawaya P | | 0，，03： | 1 |
| 18 | Influence of the confinement,soil density and displacement rate on soil-geogrid interaction显示文摘 | Lopes M L Ladeira M | 1996 | Geotextiles and Geomembranes1996，14，10： | 1 |
| 19 | Non-invasive positive pressure ventilation (CPAP or bilevel NPPV) for cardio- genic pulmonary oedema显示文摘 | Vital FM Ladeira MT Atallah AN | 2013 | Cochrane Database Syst Rev2013，5，00： | 1 |
| 20 | Mechanism of anion retention from EXAFS and density functional calculations:arsenic (V) adsorbed on gibbsite显示文摘 | Ladeira A C Q Ciminelli V S T Duarte H A Alves M C M Ramos A Y | 2001 | Geochemistry at Cosmetic-chemistry Acta2001，65，8： | 1 |
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