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| 1 | Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability,migration and proliferation in vitro显示文摘AIM: To analyze the concentration-dependent effects of autologous serum(AS) and fetal bovine serum(FBS) on human corneal epithelial cell(HCEC) viability, migration and proliferation.METHODS: AS was prepared from 13 patients with nonhealing epithelial defects Dulbecco's modified eagle medium/Ham's F12(DMEM/F12) with 5% FBS, 0.5% dimethyl sulphoxide(DMSO), 10 ng/m L human epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in serum media: DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS or FBS. HCEC viability was analyzed using cell proliferation kit XTT, migration using a wound healing assay, proliferation by the cell proliferation enzyme-linked immunosorbent assay(ELISA) Brd U kit. Statistical analysis was performed using the generalized linear model, the values at 30% AS or 30% FBS were used as the baselines.RESULTS: HCEC viability was the highest at 30% AS or 15% FBS and the lowest at 10% AS or 30% FBS application. HCEC migration was the quickest through 30% AS or 30% FBS and the slowest through 5% AS or 5% FBS concentrations. Proliferation was the most increased through 15% AS or 5% FBS and the least increased through 30% AS or 30% FBS concentrations. HCEC viability at 10% and 15% AS was significantly worse(P=0.001, P=0.023) compared to baseline and significantly better at 15% FBS(P=0.003) concentrations. HCEC migration was significantly worse(P≤0.007) and HCEC proliferation significantly better(P<0.001) in all concentration groups compared to baseline.CONCLUSION: For the best viability of HCEC 30% AS or 15% FBS, for HCEC migration 30% AS or 30% FBS, for proliferation 15% AS or 5% FBS should be used. Therefore, we suggest the use of 30% AS in clinical practice. | Ming-Feng Wu Tanja Stachon Berthold Seitz Achim Langenbucher Nóra Szentmáry | 2017 | International Journal of Ophthalmology(English edition)2017，10，6： | 7 |
| 2 | 圆锥角膜穿透性角膜移植术后缝线相关的角膜规则程度的动态变化显示文摘目的分析圆锥角膜(KC)手术前后角膜规则程度的变化,以及角膜缝线拆除对角膜规则程度的影响,对激光穿透性角膜移植手术(PK)在KC治疗中的作用进行评价。方法回顾分析了236例圆锥角膜使用激光环钻技术进行的穿透性角膜移植手术手术,使用角膜曲率计(Ophthalmometer,Type-H,190071Zeiss,Jena,Germany)对手术前后的角膜规则程度进行检测,并结合所测出的角膜曲率(K)值和角膜映光图像分成4组:(0)规则组,K〈50D;(1)轻度不规则组,K〈60;(2)中度不规则组,K≥60;(3)重度不规则组,K值不能读出,共4组。手术后第12、18个月依次拆除连续缝线,并同时随访记录各组别角膜规则程度,动态分析以上三个时间点角膜规则程度的变化特点。结果在三个时间点,规则组比例的递增趋势为4%-37%-61%轻度不规则组的变化趋势为12%-51%-33‰中度不规则组的递减趋势为38%-10%-4%;重度不规则组的变化趋势为46%-2%-2%。结论使用激光环钻和16针双线连续缝合的圆锥角膜手术病例中,穿透性角膜移植手术能有效改善病人的角膜表面规则程度,与缝线拆除相关的角膜规则程度呈动态变化,该变化主要发生在轻、中度组,缝线拆除本身并不会导致术后角膜规则程度下降。 | 刘毅 Berthold Seitz Achim Langenbucher Nhung X.Nguyen Gottfried O.H.Naumann | 2007 | 中国实用眼科杂志2007，25，4： | 3 |
| 3 | Handling of computational in vitro/In vivo correlation problems by Microsoft Excel: Ⅲ, Convolution and deconvolution 显示文摘 | LANGENBUCHER F | 2003 | Eur J Pharm Biopharm2003，56，3： | 1 |
| 4 | A comparison of rebound tonometer(ICARE) with tonometer and GAT 显示文摘 | Schreiber W Vorwerk CK Langenbucher A | 2007 | Ophthalmologe2007，104，4： | 1 |
| 5 | Changes in corneal power and refraction due to sequential suture removal following nonmechanical penetrating keratoplasty in eyes with keratoconus显示文摘 | Langenbucher A Seitz B | | 0，，： | 1 |
| 6 | Underestimation of intraocular lens power for cataract sur- gery after myopic photorefractive keratectomy 显示文摘 | SEITZ B LANGENBUCHER A NGUYEN NX KUS MM KUCHI M | 1999 | Ophthal- mology1999，106，4： | 1 |
| 7 | Implantation of a new accommodative posterior chamber intraocular lens显示文摘 | Küchle M Nguyen NX Langenbucher A | 2002 | J Refract Surg2002，18，： | 1 |
| 8 | Evaluating the eye's rotational stability during standard photography:effect on determining the axial orientation of toric intraocular lenses显示文摘 | Viestenz A Seitz B Langenbucher A | | 0，，03： | 1 |
| 9 | Comparison of 6-month results of implantation of the 1CU accommodative intraocular lens with conventional intraocular lenses显示文摘 | Kuchle M Seitz B Langenbucher A | 2004 | Ophthalmology2004，111，2： | 1 |
| 10 | Posterior corneal curvature changes after myopic laser in situ keratomileusis显示文摘 | Seitz B Torres F Langenbucher A | 2001 | Ophthalmology2001，108，4： | 1 |
| 11 | Measurement of accommodation after implantation of an accommodating posterior chamber introcular lens显示文摘 | Langenbucher A Huber S Nguyen XN | 2003 | J Cataract Refract Surg2003，29，4： | 1 |
| 12 | Long-term results of photo-therapeutic keratectomy with 193-nm excimer laser for macularcomeal dystrophy 显示文摘 | Hafner A Langenbucher A Seitz B | 2005 | Am J Ophthalmol2005，140，3： | 1 |
| 13 | Posterior corneal curvature changes after myopic laser in situ keratomileusis显示文摘 | Seitz B Torres F Langenbucher A | 2001 | Ophthalmology2001，108，： | 1 |
| 14 | Acid-base regulatory ability of the cephalopod (Sepia o f f cinalis) in response to environmental hypercapnia显示文摘 | Gutowska M A Melzner F Langenbuch M | | 0，，03： | 1 |
| 15 | Underestimation of intraocular lens power for cataract surgery after myopic photorefractive keratectomy 显示文摘 | Seitz B Langenbucher A Nguyen NX | 1999 | Ophthalmology1999，106，4： | 1 |
| 16 | High sensitivity to chronically elevated CO2 levels in a eurybathie marine sipunculid显示文摘 | Langenbuch M Portner H O | 2004 | Aquatic Toxicology2004，70，： | 1 |
| 17 | Intraocular lena power calculation in eyes after corneal reftactive surgery显示文摘 | Seitz B Langenbucher A | 2000 | Refract Surg2000，16，： | 1 |
| 18 | Posterior corneal curvature changes after myopic laser in situ keratomileusis显示文摘 | Seitz B Torres F Langenbucher A | 2001 | Ophthoalmology2001，108，： | 1 |
| 19 | Posterior corneal curvature changes after myopic laser in situ keratomileusis 显示文摘 | Seitz B Torres F Langenbucher A | 2001 | Ophthalmology2001，108，4： | 1 |
| 20 | Measurement of accommodation after implantation of an accommodation posterior chamber intraocular lens 显示文摘 | Langenbucher A Huber S Nguyen NX et al | 2005 | Cataract Refract Surg2005，29，4： | 1 |
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