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| 1 | Sensing carboxylesterase 1 in living systems by a practical and isoformspecific fluorescent probe显示文摘Carboxylesterase 1(CES1), one of the most abundant serine hydrolases in mammals, has drawn much attentions in recent years, owing to this enzyme involves in many physiological processes via hydrolysis of both endogenous esters and xenobiotic esters. Herein, to real-time monitor the activities of CES1 in various biological systems, a practical and iso form-specific fluorescent probe was developed on the basis of the substrate preference of CES1, as well as the structural and optical properties of BODIPY dyes. After screening of a panel of BODIPY ester derivatives, probe 1 displayed the best combination of specificity,sensitivity, enzymatic kinetics and applicability for monitoring CES1 activities in real samples. This probe was successfully used to detect CESl activities in several biological systems including tissue preparations,living cells, tissue slices and zebrafish. Furthermore, the biomedical applications of probe 1 for screening of CES1 inhibitors were also demonstrated using tissue preparations or living cells as enzyme sources. In summary, a practical and broadly applicable tool for real-time monitoring CES1 in biological systems was developed and well-characterized, which held great promise for further investigations on CES1-associated drug discovery, clinical practice and fundamental research. | Lele Ding Zhenhao Tian Jie Hou Tongyi Dou Qiang Jin Dandan Wang Liwei Zou Yadi Zhu Yunqing Song Jingnan Cui Guangbo Ge | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 2 | Cylindrical cavity expansion responses in anisotropic unsaturated soils under plane stress condition显示文摘In this paper,an anisotropic critical state model for saturated soils was extended to unsaturated conditions by introducing suction into its yield function.Combining this model with soil-water characteristic curves related to porosity ratio was employed to characterize the coupled hydromechanical behavior of unsaturated anisotropic soil.Based on the plane stress condition,the problem of the cylindrical cavity expansion in unsaturated anisotropic soils was transformed into first-order differential equations using the Lagrangian description.The equations were solved as an initial value problem using the Runge-Kutta algorithm,which can reflect the soil-water retention behavior during cavity expansion.Parametric analyses were conducted to investigate the influences of overconsolidation ratio(OCR),suction,and degree of saturation on the expansion responses of a cylindrical cavity in unsaturated anisotropic soil under plane stress condition.The results show that the above factors have obvious influences on the cavity responses,and the plane strain solution tends to overestimate expansion pressure and degree of saturation but underestimates suction around the cavity compared to the proposed plane stress solution.The theoretical model proposed in this paper provides a reasonable and effective method for simulating pile installation and soil pressure gauge tests near the ground surface of the unsaturated soils. | Haohua Chen Xiaolin Weng Lele Hou Dean Sun | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，6： | 0 |
| 3 | Advances in Mathematical Modeling of NFκB Signal Transduction in Mammals显示文摘NFκB is a basic transcription factor of cell signal transduction in mammals. Mathematical modeling can be used to simulate NFκB signal transduction and investigate the molecular mechanism. The initial IκB-NFκB signal transduction model emphasized the effects of negative feedback on NFκB temporal dynamics and gene expression control,while subsequent studies are concentrated on other feedback loops,input-output behaviors of modules,crosslinking of several NFκB activation paths and NFκB oscillation. Computer technology will continue to contribute to studies of NFκB signaling pathway. Finally,several potential development trends were discussed in this paper. | Jinshan ZHAO Ke JIANG Zhenghao DAI Xiaoli REN Yumei WANG Yongping JIANG Mingtuan LIU Yonglian DAI Lele HOU Hegang LI | 2013 | Agricultural Biotechnology2013，2，4： | 0 |
| 4 | LLG2/3作为复合体的共受体参与调控花粉管破裂显示文摘文章简介在被子植物的有性生殖过程中,花粉管是精细胞的运输载体,它经历与雌方多种组织和细胞的相互作用,最终将精细胞送至雌配子体进行双受精。为了鉴定参与花粉管细胞完整性调控的其他因子,课题组在系统地分析了拟南芥中的GPI-AP基因之后,发现了两个与LRE/LLG1相似、相互之间序列又非常相似的GPI-AP基因LLG2和LLG3,这两个基因在花粉中大量表达。 | Zengxiang Ge Yuling Zhao Ming-Che Liu Liang-Zi Zhou Lele Wang Sheng Zhong Saiying Hou Jiahao Jiang Tianxu Liu Qingpei Huang Junyu Xiao Hongya Gu Hen-Ming Wu Juan Dong Thomas Dresselhaus Alice Y.Cheung 瞿礼嘉 | 2020 | 科学新闻2020，，2： | 0 |
| 5 | Application of Artificially Induced Double-strand Breaks (DSB) and Triplex-forming Oligonucleotides (TFO) in the Improvement of Gene Targeting Efficiency显示文摘Gene targeting technology is an important means to investigate gene functions,but its efficiency of gene targeting is very low,especially for somatic cell targeting. Artificially induced double-strand breaks (DSB) and triplex forming oligonucleotide (TFO) are currently developed methods to improve the targeting efficiency. This paper summarized the basic principles,design ideas and application in gene targeting efficiency improvement of these two methods,analyzed and compared their characteristics,and finally proposed prospects for their future development. | Hegang LI Wenke CHENG Ke JIANG Xiaoli REN Yongping JIANG Lele HOU Xiaojing HAO Jinshan ZHAO | 2013 | Agricultural Biotechnology2013，2，1： | 0 |
| 6 | Undrained semi-analytical solution for cylindrical cavity expansion in anisotropic soils under biaxial stress conditions显示文摘This paper presents an undrained semi-analytical elastoplastic solution for cylindrical cavity expansion in anisotropic soil under the biaxial stress conditions.The advanced simplified SANICLAY model is used to simulate the elastoplastic behavior of soil.The cavity expansion is treated as an initial value problem and solved as a system of eight first-order ordinary differential equations including four stress components and four anisotropic parameters.The results are validated by comparing the new solutions with existing ones.The distributions of stress components and anisotropic parameters around the cavity wall,the expansion process,the stress yield trajectory of a soil element and the shape and size of elastoplastic boundary are further investigated to explore the cavity expansion response of soils under biaxial in situ stresses.The results of extensive parameters analysis demonstrate that the circumferential position of the soil element and the anisotropy of the soils have noticeable impacts on the expansion response under biaxial in situ stresses.Since the present solution not only considers the anisotropy and anisotropy evolution of natural soil,but also eliminates the conventional assumption of uniform radial pressure,the solution is better than other theoretical solutions to explain the pressure test and pile installation effect of shallow saturated soil. | Lele Hou Xiaolin Weng Jibo Hu Rongming Zhou | 2023 | Journal of Rock Mechanics and Geotechnical Engineering2023，15，5： | 0 |
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