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| 1 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 2 | Risk factors and natural history of breast cancer in younger Chinese women显示文摘AIM: To investigate the age differences in the risk factors, clinicopathological characteristics and patterns of treatment of female breast cancer patients. METHODS: Seven thousand one hundred and fiftytwo women with primary breast cancer from the Hong Kong Breast Cancer Registry were recruited after receiving patients' consent, they were asked to complete standardized questionnaires which captured their sociodemographic characteristics and risk factors associated with breast cancer development. Among them, clinicopathological data and patterns of treatment were further collected from medical records of 5523 patients with invasive breast cancers. Patients were divided into two groups according to the age at diagnosis: younger(< 40 years old) vs older patients(≥ 40 years old) for subsequent analyses.RESULTS: Analysis on the sociodemographic characteristics and exposure to risk factors were performed on 7152 women with primary breast cancer and the results revealed that younger patients were more likely to have unhealthy lifestyles; these include a lack of exercise(85.4% vs 73.2%, P < 0.001), having high stress in life(46.1% vs 35.5%, P < 0.001), having dairy/meat-rich diets(20.2% vs 12.9%, P < 0.001),having alcohol drinking habit(7.7% vs 5.2%, P = 0.002). Younger patients were also more likely to have hormone-related risk factors including nulliparity(43.3% vs 17.8%, P < 0.001) and an early age at menarche(20.7% vs 13.2%, P < 0.001). Analyses on clinicopathological characteristics and patterns of treatment were performed on 5523 women diagnosed with invasive breast cancer. The invasive tumours in younger patients showed more aggressive pathological features such as having a higher percentage of grade 3 histology(45.7% vs 36.5%, P < 0.001), having a higher proportion of tumours with lymphovascular invasion(39.6% vs 33.2%, P = 0.003), and having multifocal disease(15.7% vs 10.3%, P < 0.001); they received different patterns of treatment than their older counterparts.CONCLUSION: Younger patients in Hong Kong are more likely to encounter risk factors associated with breast cancer development and have more aggressive tumours than their older counterparts. | Winnie Yeo Hang-Mei Lee Amy Chan Emily YY Chan Miranda CM Chan Keeng-Wai Chan Sharon WW Chan Foon-Yiu Cheung Polly SY Cheung Peter HK Choi Josette SY Chor William WL Foo Wing-Hong Kwan Stephen CK Law Lawrence PK Li Janice WH Tsang Yuk Tung Lorna LS Wong Ting-Ting Wong Chun-Chung Yau Tsz-Kok Yau Benny CY Zee | 2014 | World Journal of Clinical Oncology2014，5，5： | 4 |
| 3 | Time-intensity based volumetric flow measurements:an in vitro study显示文摘 | Li PC Yeh CK Wang SW | 2002 | Ultrasound Med Biol2002，28，3： | 1 |
| 4 | Characterization of bone and soft-tissue tumors with in vivo 1 H-MR spectroscopy:initial results显示文摘 | Wang CK Li CW Hsieh TJ | 2004 | Radiology2004，232，： | 1 |
| 5 | Elevated production of interleukin-18 is associated with renal disease in patients with systemic lupus erythematosus显示文摘 | Wong CK Ho CY Li EK | 2002 | Clin Exp Immunol2002，130，2： | 1 |
| 6 | Soluble human p55 and p75 tumor necrosis factor receptors reverse spontaneous arthritis in transgenie mice expressing transmembrane tumor necrosis factor alpha显示文摘 | Edwards CK Bendele AM Reznikov LI | 2006 | Arthritis Rheum2006，54，9： | 1 |
| 7 | Protein kinase PKR and RNA adenosine deaminase ADAR1 : new roles for old players as modu|ators of the interferon response 显示文摘 | Pfaller CK Li Z George CX | 2011 | Curr Opin Immunol2011，23，5： | 1 |
| 8 | Elevation of proinflammatory cytokine (IL-18, IL-17, IL-12) and Th2 eytokine (IL-4) concentrations in patients with systemic lupus erythematous 显示文摘 | Wong CK Ho CY Li EK | 2000 | Lupus2000，9，： | 1 |
| 9 | Elevated plasma concentrations of nitric oxide, soluble thrombomodul in and soluble vascular cell adhesion molecule- 1 in patients with systemic lupus erythematosus显示文摘 | Ho CY Wong CK Li EK | 2003 | Rheumatology (Oxford)2003，42，1： | 1 |
| 10 | Safety and efficacy of leflunomide in the treatment of lupus nephritis refractory or intolerant to traditional immunosuppressive therapy: an open label trial 显示文摘 | Tam IS Li EK Wong CK | 2006 | Ann Rheum Dis2006，65，3： | 1 |
| 11 | Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis显示文摘 | Li N Mruk DD Wong CK | 2015 | FASEB J2015，29，9： | 1 |
| 12 | 显示文摘 | Ho CY Wong CK Li EK | 2001 | Immunol Invest2001，30，3： | 1 |
| 13 | A Chinese adolescent girl with Fechtner-like syndrome显示文摘 | Leung TF Tsoi WC Li CK | 1998 | Acta Paediatr1998，87，： | 1 |
| 14 | The cytokines IL-21 and GM-CSF have opposing regulatory roles in the apoptosis of conventional dendritic cells 显示文摘 | Wan CK Oh J Li P | 2013 | Immunity2013，38，3： | 1 |
| 15 | Immunomodulatory effect of the anti-asthma Chinese herbal formula,MSSM-002 on Th2 cells显示文摘 | Srivastava K Teper AA Zhang TF Li S Walsh MJ Huang CK Kattan M Schofield BH Sampson HA Li XM | | 0，，02： | 1 |
| 16 | Single vs twice daily G-CSF dose for peripheral blood stem cells harvest in normal donors and chil- dren with non-malignant diseases 显示文摘 | Lee V Li CK Shing MM | 2000 | Bone Marrow Transplant2000，25，9： | 1 |
| 17 | The chinese herbal medicine formula MSSM-0 02 suppresses allergic airway hyperreactivity and modulates TH1/TH2 responses in a murine model of allergic asthma显示文摘 | Li XM Huang CK Zhang TF | 2000 | J Allergy Clin Immunol2000，106，4： | 1 |
| 18 | Oxidized LDL reduces monocyte CCR2 expression through pathways involving peroxisome proliferator-activated receptor gamma显示文摘 | Han KH Chang MK Boullier A Green SR Li A Glass CK | 2000 | J Clin Invest2000，106，6： | 1 |
| 19 | Sphingosine 1-phosphate SIP2 and S1 P3 receptor-mediated Akt activation protects against in vivo myocardial ischemia-reperfusion injury 显示文摘 | Means CK Xiao CY Li Z | 2007 | Am J Physiol Heart Circ Physiol2007，292，： | 1 |
| 20 | Evaluation of the eficacy of treat-ment of Kawasaki disease before day 5 of illness显示文摘 | Fong NC Hui Yw Li CK | 2004 | Pediatri Cardiol2004，25，1： | 1 |
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