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| 1 | Long-term Traffic Volume Prediction Based on K-means Gaussian Interval Type-2 Fuzzy Sets显示文摘This paper uses Gaussian interval type-2 fuzzy se theory on historical traffic volume data processing to obtain a 24-hour prediction of traffic volume with high precision. A K-means clustering method is used in this paper to get 5 minutes traffic volume variation as input data for the Gaussian interval type-2 fuzzy sets which can reflect the distribution of historical traffic volume in one statistical period. Moreover, the cluster with the largest collection of data obtained by K-means clustering method is calculated to get the key parameters of type-2 fuzzy sets, mean and standard deviation of the Gaussian membership function.Using the range of data as the input of Gaussian interval type-2 fuzzy sets leads to the range of traffic volume forecasting output with the ability of describing the possible range of the traffic volume as well as the traffic volume prediction data with high accuracy. The simulation results show that the average relative error is reduced to 8% based on the combined K-means Gaussian interval type-2 fuzzy sets forecasting method. The fluctuation range in terms of an upper and a lower forecasting traffic volume completely envelopes the actual traffic volume and reproduces the fluctuation range of traffic flow. | Runmei Li Yinfeng Huang Jian Wang | 2019 | IEEE/CAA Journal of Automatica Sinica2019，6，6： | 8 |
| 2 | Characterization of glucose-6-phosphate dehydrogenase deficiency and identification of a novel haplotype 487G>A/IVS5-612(G>C) in the Achang population of southwestern China显示文摘The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its gene mutations were studied in the Achang population from Lianghe County in Southwestern China. We found that 7.31% (19 of 260) males and 4.35% (10 of 230) females had G6PD deficiency. The molecular analysis of G6PD gene exons 2―13 was performed by a PCR-DHPLC-Sequencing or PCR-Sequencing. Sixteen inde-pendent subjects with G6PD Mahidol (487G>A) and the new polymorphism IVS5-612 (G>C), which combined into a novel haplotype, were identified accounting for 84.2% (16/19). And 100% Achang G6PD Mahidol were linked to the IVS5-612 C. The percentage of G6PD Mahidol in the Achang group is close to that in the Myanmar population (91.3% 73/80), which implies that there are some gene flows between Achang and Myanmar populations. Interestingly, G6PD Canton (1376G>T) and G6PD Kaiping (1388G>A), which were the most common G6PD variants from other ethnic groups in China, were not found in this Achang group, suggesting that there are different G6PD mutation profiles in the Achang group and other ethnic groups in China. Our findings appear to be the first documented report on the G6PD genetics of the AChang people, which will provide important clues to the Achang ethnic group origin and will help prevention and treatment of malaria in this area. | YANG YinFeng, ZHU YueChun, LI DanYi, LI ZhiGang, Lü HuiRu, WU Jing, TANG Jing & TONG ShuFen Department of Biochemistry, Faculty of Basic Medicine, Kunming University of Medical Sciences, Kunming 650031, China These authors contributed equally to this work | 2007 | Science China(Life Sciences)2007，50，4： | 6 |
| 3 | A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus显示文摘Amphibian metamorphosis provides a wonderful model to study the thyroid hormone(TH)signaling disrupting activity of environmental chemicals, with Xenopus laevis as the most commonly used species. This study aimed to establish a rapid and sensitive screening assay based on TH-response gene expression analysis using Pelophylax nigromaculatus, a native frog species distributed widely in East Asia, especially in China. To achieve this, five candidate TH-response genes that were sensitive to T3 induction were chosen as molecular markers, and T3 induction was determined as 0.2 nmol/L T3 exposure for 48 hr. The developed assay can detect the agonistic activity of T3 with a lowest observed effective concentration of 0.001 nmol/L and EC50 at around 0.118–1.229 nmol/L, exhibiting comparable or higher sensitivity than previously reported assays. We further validated the efficiency of the developed assay by detecting the TH signaling disrupting activity of tetrabromobisphenol A(TBBPA), a known TH signaling disruptor. In accordance with previous reports, we found a weak TH agonistic activity for TBBPA in the absence of T3,whereas a TH antagonistic activity was found for TBBPA at higher concentrations in the presence of T3, showing that the P. nigromaculatus assay is effective for detecting TH signaling disrupting activity. Importantly, we observed non-monotonic dose-dependent disrupting activity of TBBPA in the presence of T3, which is difficult to detect with in vitro reporter gene assays. Overall, the developed P. nigromaculatus assay can be used to screen TH signaling disrupting activity of environmental chemicals with high sensitivity. | Yinfeng Zhang Yuanyuan Li Zhanfen Qin Huili Wang Jianzhong Li | 2015 | Journal of Environmental Sciences2015，27，8： | 4 |
| 4 | Optimization of the T3-induced Xenopus metamorphosis assay for detecting thyroid hormone signaling disruption of chemicals显示文摘T3-induced Xenopus metamorphosis is an ideal model for detecting thyroid hormone(TH)signaling disruption of chemicals. To optimize the T3-induced Xenopus assay and improve its sensitivity and reproducibility, we intend to develop quantitatively morphological endpoints and choose appropriate concentrations and exposure durations for T3 induction.Xenopus laevis at stage 52 were exposed to series of concentrations of T3(0.31–2.5 nmol/L)for 6 days. By comparing morphological changes induced by T3, we propose head area,mouth width, unilateral brain width/brain length, and hindlimb length/snout-vent length as quantitative parameters for characterizing T3-induced morphological changes, with body weight as a parameter for indicating integrated changes. By analyzing time-response curves, we found that following 4-day exposure, T3-induced grossly morphological changes displayed linear concentration–response curves, with moderate morphological changes resulting from 1.25 nmol/L T3 exposure. When using grossly morphological endpoints to detect TH signaling disruption, we propose 4 days as exposure duration of T3, with concentrations close to 1.25 nmol/L as induction concentrations. However, it is appropriate to examine morphological and molecular changes of the intestine on day 2 due to their early response to T3. The quantitative endpoints and T3 induction concentrations and durations we determined would improve the sensitivity and the reproducibility of the T3-induced Xenopus metamorphosis assay. | Xiaofang Yao Xiaoying Chen Yinfeng Zhang Yuanyuan Li Yao Wang Zongming Zheng Zhanfen Qin Qingdong Zhang | 2017 | Journal of Environmental Sciences2017，29，2： | 2 |
| 5 | Expression of active recombinant human lactoferrin in the milk of transgenic goats显示文摘 | ZHANG Jie LI Lin CAI Yinfeng | 2008 | Protein Expr Purif2008，57，： | 1 |
| 6 | Preparation and Formation Process of Ni2+Fe3+CO32- LDHs Materials with High Crystallinity and Well-Defined Hexagonal Shapes显示文摘 | Han Yinfeng Li Hongjuan Ma Xiangrong | 2009 | Solid State Sciences2009，11，12： | 1 |
| 7 | Journal of the Chinese Rare Earth Society 显示文摘 | Sun Yinfeng Li Guodong Zhang Changzai | 2006 | 24 ( zl ) : 152- 1552006，24，： | 1 |
| 8 | Nutrient Removal from Polluted River Water by Using Constructed Wetlands显示文摘 | Jin Shunren Lin Yinfeng Li Deyuan | 2011 | Bioresource Technology2011，，76： | 1 |
| 9 | On the on-line rent-or-buy problem in probabilistic environments显示文摘 | Xu Yinfeng Xu Weijun Li Hongyi | | 0，，1： | 1 |
| 10 | Electrochemical behavior of codeine and its sensitive determination on graphene-based modified electrode 显示文摘 | Yinfeng Li Kunjing Li Ge Song | 2013 | Sensors and Actua- tors B2013，182，： | 1 |
| 11 | A Mediator- free Phenol Biosensor Basedon Immobilizing Tyrosinase to ZnO Nanoparticles显示文摘 | Li Yinfeng Liu Zhimin Liu Yanli | 2006 | Anal Biochem2006，349，1： | 1 |
| 12 | A multiscale latent dirichlet allocation model for object-oriented clustering of VHR panchromatic satellite images显示文摘 | Tang Hong Shen Li Qi Yinfeng | 2013 | IEEE Trans on Geos- cience and Remote Sensing2013，51，3： | 1 |
| 13 | Core - shell magnetic nanoparticles applied for immobilization of an- tibody on carbon paste electrode and amperometric im- munosensing 显示文摘 | Liu Zhimin Yang Haifeng Li Yinfeng | 2006 | Sens Aetutor B2006，113，： | 1 |
| 14 | Expression of active recombinant human lactoferrin in the milk of transgenic goats显示文摘 | Zhang Jie Li Lin Cai Yinfeng | | 0，，： | 1 |
| 15 | Complication following gastric pull-up reconstruction for advanced hypopharyngeal or cervical esophageal carcinoma: a 20-year review in a Chinese institute显示文摘 | He Shuangba Sun Jingwu Wang Yinfeng Hu Yanming Lv Qiuping Li Xianguang Xu Weiqing Wang Shengjun Yu Zhenkun | 2011 | American Journal of Otolaryngology--Head and Neck Medicine and Surgery2011，，4： | 1 |
| 16 | On consisten cy measures of linguistic preference relations显示文摘 | Dong Yucheng Xu Yinfeng Li Hongyi | 2008 | Euro pean Journal of Operational Research2008，189，2： | 1 |
| 17 | On consistency measures of linguistic preference relations 显示文摘 | Dong Yucheng Xu Yinfeng Li Hongyi | 2008 | European Journal of Operational Research2008，189，43： | 1 |
| 18 | On the on-line rent-or- buy problem in probabilistic environments 显示文摘 | Xu Yinfeng Xu Weijun Li Hongyi | 2007 | Journal of Global Optimization2007，38，1： | 1 |
| 19 | Preparation and re- search on performance of fluorine rubber/metal composite seal- ing boards显示文摘 | Zhang Ailing Feng Yinfeng Li Sanxi | 2012 | Advanced Engineering Materials I12012，535，： | 1 |
| 20 | On consistency measures of linguistic preference relations显示文摘 | Yucheng Dong Yinfeng Xu Hongyi Li | 2008 | European Journal of Operational Research2008，189，2： | 1 |
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