| 1 | Over-expression of an Arabi-dopsisd δ-OAT gene enhances salt and drought tolerance in transgenic rice显示文摘d-OAT, ornithine--aminotransferase, is the key enzyme involved in proline biosynthesis. In this study the Arabidopsis d -OAT gene was transferred into rice (Oryza sativa L. ssp japonica cv. Zhongzuo 321), whose successful integration was demonstrated by PCR and Southern blot analysis. The over-expression of the gene in transgenic rice was also confirmed. Biochemical analysis showed that, under salt or drought stress conditions, proline contents in the leaves and roots in transgenic rice plants were 5- to 15-fold of those in non-transgenic controls. Under stress conditions, germinating rate of transgenic lines is higher than that of controls. Although the growth of rice plants tested were more and more retarded with the increasing of NaCl concentration, the transgenic plants grow faster compared to the controls under the same stress condition. Meanwhile, the resistance to KCl and MgSO4 stresses was also found enhanced in trans-genic rice. Furthermore, the over-expression of d -OAT also improved the yield of transgenic plants under stress condi-tions. The average yield per plant of transgenic lines in-creases about 12%—41% more than that of control lines under 0.1 mol/L NaCl stress. These data indicated that the over-expression of d -OAT, with the accumulation of proline, resulted in the enhancement of salt and drought tolerance and an increase of rice yield, which is of significance in agri-culture. | WU Liangqi, FAN Zhanmin, GUO Lei, LI Yongqing, ZHANG Wenjing, QU Li-Jia & CHEN Zhangliang The National Laboratory of Protein Engineering and Plant Genetic En-gineering, College of Life Sciences, Peking University, Beijing 100871, China Correspondence should be addressed to Qu Li-Jia (e-mail: qulj@pku. edu.cn) | 2003 | Chinese Science Bulletin2003，48，23： | 11 |
| 2 | Recent advances in photodynamic therapy based on emerging two-dimensional layered nanomaterials显示文摘Photodynamic therapy(PDT)is a promising non-invasive therapy approach for various diseases including malignant tumor.The process of PDT involves three interrelated aspects,namely photosensitizer(PS),light source,and oxygen,among which PS is the decisive factor that determines its anticancer efficiency.There exist some defects in currently applied PDT,such as inadequate production of reactive oxygen species(ROS),poor penetration of exciting light,insufficient oxygen supply,and nonselective distribution of PS.With unique physicochemical and optical properties,two-dimensional nanomaterials(2DNMs)have aroused great interest in biomedical fields.2DNMs-based PDT is promising to significantly improve antitumor efficacy compared to conventional PDT.In this review,we will firstly introduce the underlying mechanism of PDT and how 2DNMs are absorbed and distribute inside tumor cells.After that,we will not only illustrate how 2DNMs-based PDT can enhance tumor-killing efficacy and minimize side-effects through conquering the above-mentioned defects of conventional PDT and the preparation process of 2DNMs,but also elaborate recent advances about 2DNMs-based PDT.Lastly,we will summarize the challenges and future prospects of 2DNMs-based PDT. | Xinqiang Wu Xiaofeng Jiang Taojian Fan Zhiwei Zheng Zhaoyuan Liu Yubin Chen Liangqi Cao Zhongjian Xie Dawei Zhang Jiaqi Zhao Qiwen Wang Zhenhui Huang Zhijian Chen Ping Xue Han Zhang | 2020 | Nano Research2020，13，6： | 6 |
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