| 1 | Over-expression of an Arabi-dopsisd δ-OAT gene enhances salt and drought tolerance in transgenic rice显示文摘d-OAT, ornithine--aminotransferase, is the key enzyme involved in proline biosynthesis. In this study the Arabidopsis d -OAT gene was transferred into rice (Oryza sativa L. ssp japonica cv. Zhongzuo 321), whose successful integration was demonstrated by PCR and Southern blot analysis. The over-expression of the gene in transgenic rice was also confirmed. Biochemical analysis showed that, under salt or drought stress conditions, proline contents in the leaves and roots in transgenic rice plants were 5- to 15-fold of those in non-transgenic controls. Under stress conditions, germinating rate of transgenic lines is higher than that of controls. Although the growth of rice plants tested were more and more retarded with the increasing of NaCl concentration, the transgenic plants grow faster compared to the controls under the same stress condition. Meanwhile, the resistance to KCl and MgSO4 stresses was also found enhanced in trans-genic rice. Furthermore, the over-expression of d -OAT also improved the yield of transgenic plants under stress condi-tions. The average yield per plant of transgenic lines in-creases about 12%—41% more than that of control lines under 0.1 mol/L NaCl stress. These data indicated that the over-expression of d -OAT, with the accumulation of proline, resulted in the enhancement of salt and drought tolerance and an increase of rice yield, which is of significance in agri-culture. | WU Liangqi, FAN Zhanmin, GUO Lei, LI Yongqing, ZHANG Wenjing, QU Li-Jia & CHEN Zhangliang The National Laboratory of Protein Engineering and Plant Genetic En-gineering, College of Life Sciences, Peking University, Beijing 100871, China Correspondence should be addressed to Qu Li-Jia (e-mail: qulj@pku. edu.cn) | 2003 | Chinese Science Bulletin2003，48，23： | 11 |
| 3 | Damage Symptoms and Damage Degree of Main Diseases and Pests in Foxtail Millet in Chengde Area显示文摘The species of main diseases and pests in foxtail millet and their damage status were investigated in 15 survey points in Longhua, We-ichang, Chengde, Fengning and Luanping counties, Chengde City from 2015 to 2017. It was preliminarily cleared that there were 11 species of main diseases and 8 species of pests in continuous cropping millet field. The diseases damaged severely were millet downy mildew and kernel smut, and pests were yellow-legged lema, millet shoot fly and soil insects. The results provided a scientific basis for green control of millet pests and diseases and safe production in Chengde area. | Fan Qingjie Zhang Yingying Shi Aili Xing Zhanmin Kang Liang | 2018 | Plant Diseases and Pests2018，9，1： | 0 |
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