| 1 | Low-frequency electrical stimulation improves neurite outgrowth of dorsal root ganglion neurons in vitro via upregulating Ca^(2+)-mediated brain-derived neurotrophic factor expression显示文摘Short-term, low-frequency electrical stimulation of neural tissues significantly enhances axonal regeneration of peripheral nerves following injury. However, little is known about the mechanisms of electrical stimulation to induce neurite outgrowth. In the present study, short-term, low-frequency electrical stimulation, using identical stimulation parameters of in vivo experiments, was administered to in vitro dorsal root ganglion (DRG) neurons. Enhanced neurite outgrowth, as well as synthesis and release of brain-derived neurotrophic factor (BDNF), were examined in electrical stimulation-treated DRG neuronal cultures. Because the effects of electrical stimulation on neuronal intracellular signaling molecules are less reported, classic calcium intracellular signals are directly or indirectly involved in electrical stimulation effects on neurons. Cultured DRG neurons were pretreated with the calcium channel blocker nifedipine, followed by electrical stimulation. Results suggested that electrical stimulation not only promoted in vitro neurite outgrowth, but also enhanced BDNF expression. However, nifedipine reduced electrical stimulation-enhanced neurite outgrowth and BDNF biosynthesis. These results suggest that the promoting effects of electrical stimulation on DRG neurite outgrowth could be associated with altered calcium influx, which is involved induction of neuronal BDNF expression and secretion. | Lidan Wan Rong Xia Wenlong Ding | 2010 | Neural Regeneration Research2010，5，16： | 1 |
| 2 | Propolis inspired sunscreens for efficient UV-protection and skin barrier maintenance显示文摘The direct use of naturally occurring,small molecular ingredients in bioinspired sunscreens has raised several concerns due to the instability,photocytotoxicity,and potential blood toxicity of those ingredients.In this work,we have employed natural ultraviolet(UV)-blocking molecule caffeic acid phenethyl ester(CAPE)from propolis to prepare poly(CAPE)nanoparticles(NPs)as the main bioactive ingredient to fabricate propolis-inspired hydrogel sunscreens.Compared with small molecular CAPE,poly(CAPE)NPs exhibited better dispersion and stability in water,as well as lower physiological toxicity and skin permeability.And the resulting composite hydrogels demonstrated promising properties including water-resistant whereas can be easily erased by warm water as well as safety when interacting with skin.More importantly,the hydrogel sunscreens showed excellent UV protection properties both in vitro and in vivo,and the positive effects in maintaining skin barrier functions.This work provides new strategies towards the facile construction of nature-inspired robust sunscreens in the future. | Yu Fu Ruoyu Wan Lei Yang Lidan Xiong Junfei Hu Jie Tang Hailun He Zhipeng Gu Li Li Yiwen Li | 2022 | Nano Research2022，15，9： | 0 |
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